Objective: To investigate the effect of oxidized low-density lipoprotein (ox-LDL) on expression of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1), transforming growth factor beta 1 (TGF-β 1), and secretion of extracelluar matrix (ECM) in cultured rat glomerular mesangial cells (GMCs), and to investigate the influence of LOX-1 inhibitor polyinosinic acid (PIA) on the effect of ox-LDL. Methods: Rat glomerular masengial cells were cultured in vitro. RT-PCR was employed to determine the LOX-1 mRNA expression in GMCs incubated with different concentrations of ox-LDL (0, 25, 50, 100 μg/ ml). The expression of LOX-1 and TGF-β1, mRNA was also determined by RT-PCR in the blank control group, ox-LDL (50 μg/ml) group and PIA(50 μg/ml ox-LDL+250 μg/ml PIA) group. The contents of TGF-β1 fibronectin (FN), and collagen IV (Col IV) in the supernatants of the above 3 groups were determined by ELISA. Results: RT-PCR showed that LOX-1 mRNA expression in 25, 50 and 100 μg/ml ox - LDL groups was significantly higher than that of blank control group(P<0.05), with the highest expression found in the 50 μg/ml ox-LDL group; the expression of LOX-I and TGF-β 1 mRNA was significantly higher in 50 μg/ml ox-LDL group than that in the other 2 groups (P< 0.01). ELISA results demonstrated that the supernatant contents of TGF-β1, FN and Col IV were significantly higher in 50 μg/ml ox-LDL group than those in the other 2 groups (P< 0.05). Conclusion: Ox-LDL can upregulate the expression of LOX-1 and TGF-β1, mRNA and the secretion of extracelluar matrix in GMCs. Polyinosinic acid can antagonize the above effect of ox-LDL, suggesting that LOX-1 may participate in ox-LDL-induced GMCs damage and is involved in the development and progression of glomerulosclerosis.

Objective: To explore the effect of silent information regulator 1(SIRT1) on high glucose-induced nuclear factor-κB (NF-κB) p65 subunit acetylation and monocyte chemoattractant protein 1 (MCP-1) expression in rat mesangial cells (RMCs). Methods: The lentiviral shRNA plasmid pTRC-shSIRT1 was constructed for interference of SIRT1 gene and was identified. The RMCs were divided into high glucose group (treated with high glucose culture medium), resveratrol (SIRT1 activator)+high glucose group (treated with low glucose culture medium containing 1 μmol/L resveratrol for 24 h, and then with high glucose culture medium), SIRT1 RNAi group (4 h after viral pTRC-shSIRT1 infection, and then treated with low-glucose culture medium), SIRT1 RNAi + high glucose group (4 h after viral pTRC-shSIRT1 infection, and then treated with high glucose culture medium); we also established normal control group and hypertonic mannitol control group. The mRNA expression of SIRT1 and MCP-1 gene was analyzed by real-time quantitative PCR; the protein expression of SIRT1 and the acetylation of NF-κB p65 subunit were observed by Western blotting analysis. The protein level of MCP-1 in the supernatants was detected by ELISA. Results: DNA sequencing confirmed the successful construction of the plasmid pTRC-shSIRT1, which could knocked down SIRT1 mRNA expression(P<0.01). High glucose decreased SIRT1 expression and promoted acetylation of NF-κB p65 subunit, and increased MCP-1 mRNA and protein expression. Resveratrol, an activator of SIRT1, could reverse the above changes induced by high glucose. Conversely, silencing SIRT1 gene significantly accelerated the high glucose-induced acetylation of NF-κB p65 subunit and MCP-1 expression at both mRNA and protein levels(P<0.01 or P<0.05). Conclusion: SIRT1 can inhibit high glucose-induced MCP-1 mRNA and protein expression in RMCs, which may involve NF-κB p65 deacetylation.

Objective To investigate the diagnostic value and significance of myocardial injury markers in neonates.Methods A retrospective analysis of 838 cases of myocardial injury markers in neonates,the total positive rate of hs -cTnI and the positive rates of hs -cTnI(hypersensitivity cardiac troponin I)in different kinds of neonatal disease were calculated,the levels of abnormalities consistent rate in hs -cTnI and CK -MBmass(creatine kinase MB mass)were compared with hs -cTnI and MYO(Myoglobin).Results The total positive rate of hs -cTnI was 40.33% in the 838 neonates.The highest incidence of myocardial injury was neonatal sepsis (57.14%),followed by neonatal pulmonary hemorrhage (55.56%)and neonatal convulsions (54.55%).The abnormalities consistent rate of hs -cTnI and CK -MBmass was better than hs -cTnI and MYO (85.50% vs 28.11%,χ2 =226.9,P <0.05). Conclusion Neonatal hospitalized children often complicated by myocardial injury;As a biochemical myocardial injury marker,the hs -cTnI detection is important for early detection of myocardial injury,it should be recommend as routine test items;CK -MBmass has better correlation with hs -cTnI than MYO,which can provide guide for doctors to interpret the data of myocardial injury markers.

OBJECTIVETo investigate the effects of Tongfu Huoxue decoction on experimental intracelebral hemorrhage and the associated machenisms.

METHODThe cerebral hemorrhage model in rats was induced by local injection of type VII collagenase and they were randomly divided into four groups. The treated groups were treated with Naoxuekang and Tongfu Huoxue decoction. The control groups were only treated with water. The changes of neurological defect were observed. The content of brain water, MDA, NO and the activity of SOD were measured.

RESULTThe cerebral hemorrhage rats showed hemiplegia, and the hemorrhage brains showed celebral edema, higher quotient of brain and content of brain water, suggesting the hemorrhage model was established successfully. After the treatment of Tongfu Huoxue decoction, the hemorrhage rats showed smaller hemorrhage volume, the brain tissue from the hemorrhage rats had lower MDA content and the quotient of brain, and also had higher activity of SOD and content of NO.

CONCLUSIONTongfu Huoxue decoction has treatment effects on cerebral hemorrhage.

Animals ; Arctium ; chemistry ; Brain ; metabolism ; pathology ; Cerebral Hemorrhage ; chemically induced ; drug therapy ; metabolism ; pathology ; Collagenases ; Drug Combinations ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; therapeutic use ; Fallopia japonica ; chemistry ; Male ; Malondialdehyde ; metabolism ; Nitric Oxide ; metabolism ; Phytotherapy ; Plants, Medicinal ; chemistry ; Prunus ; chemistry ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Superoxide Dismutase ; metabolism

OBJECTIVETo search for the possible relation between tortilcollis and partial chromosome 13q trisomy.

METHODSFluorescence in situ hybridization (FISH) technique combined with chromosome banding was performed to determine the karyotype of two patients with typical clinical features of partial 13q trisomy syndrome, then their manifestations were compared with those of the literatures published previously.

RESULTSThe two cases were partial trisomy of 13q14--> ter with a different second derivative chromosome, in spite of this difference, both of them had tortilcollis.

CONCLUSIONIt is suggested that a potential site for tortilcollis may locate on the long arm of chromosome 13. With reference to a report previously published, the more precise candidate related region may be 13q32--> qter.

Child ; Chromosome Banding ; Chromosome Deletion ; Chromosomes, Human, Pair 13 ; genetics ; Cytogenetics ; Humans ; In Situ Hybridization, Fluorescence ; Karyotyping ; Male ; Torticollis ; genetics ; Trisomy ; genetics

AIM:To observe the effect of Tangshenfang(TS)on the liver protection and the levels of silent in-formation regulator 1(SIRT1)and peroxisom proliferator-activated receptor γcoactivator-1α(PGC-1α)in the liver tissue. METHODS:The rat model of diabetes mellitus(DM)was established by intravenous injection of streptozotocin(STZ;30 mg/kg)after having the high fat/high glucose diets for 1 month.The diabetic rats were randomly divided into DM group,DM with high-dose TS(TSHi)group, medium-dose TS(TSMed)group and low-dose TS(TSLow)group.The normal rats were served as control group.There were 8 rats in each group.After treatment with TS for 12 weeks,the serum biochemical indi-ces including fasting blood glucose(FBG), triglyceride(TG), alanine aminotransferase(ALT)and aspartate aminotrans-ferase(AST)were tested.Fasting insulin(FINS)was also detected by radioimmunoassay,and homeostatic model assess-ment for insulin resistance(HOMA-IR)was calculated.The serum levels of tumor necrosis factor-α(TNF-α)and interleu-kin-1(IL-1)were measured by ELISA.The activity of SOD and content of MDA in the liver tissues were measured by the methods of hydroxylamine and thiobarbituric acid.The liver pathological changes were observed under light microscope with HE and Masson staining.The protein expression of SIRT1and PGC-1αin the liver tissues was determined by Western blot. RESULTS:In DM group,serum FBG,TG,ALT,AST,FINS,HOMA-IR,TNF-αand IL-1 were obviously increased com-pared with the control group(P<0.01).The fatty changes,local necrosis,inflammation and fibrosis in the liver tissues were observed.The content of MDA in liver increased,while the activity of SOD decreased markedly.The protein expression of SIRT1 and PGC-1αwas decreased(P<0.05).In TS treatment groups,all these changes in DM rats were markedly reversed by TS,and the protein expression of SIRT1 and PGC-1αin the liver tissues was markedly increased.CONCLUSION:TS may protect the rats from diabetic liver injury by increasing the expression of SIRT 1 and PGC-1α,and thereby improving in-sulin resistance and oxidative stress.

Objective:To investigate the intervention effect of panax notoginseng saponins (PNS) on epithelial-mesenchymal transition (EMT) of rat renal tubular epithelial cells (NRK-52E) induced by transforming growth factor-β₁(TGF-β₁), and analyze the mechanism based on the silent information regulation 2 homolog 1(SIRT1)/TGF-β₁/Smad signaling pathway. Method:NRK-52E were cultured in DMEM medium with 10% fetal bovine serum, and divided into normal control group, TGF-β₁ group (5 μg·L^-1), resveratrol (RSV) group (50 mg·L^-1), EX527 group (10 μmol·L^-1), Panax notoginseng saponins (PNS) group (100 mg·L^-1), and EX527+ PNS group (10 μmol·L^-1+100 mg·L^-1). Then cells were collected after drug intervention for 48 h. The expressions of α-SMA,E-cadherin,SIRT1,TGF-β₁,Smad3,Smad4 mRNA in each group were detected by Real-time PCR. The protein expressions of α-SMA, E-cadherin,SIRT1 and TGF-β₁ were detected by Western blot. Result:Compared with normal group, mRNA and protein expressions of α-SMA increased obviously(P<0.05), but E-cadherin decreased significantly(P<0.01)in TGF-β₁ group. Compared with TGF-β₁ group, mRNA and protein expressions of α-SMA decreased significantly(P<0.01), while E-cadherin increased(P<0.01)in resveratrol and PNS groups, and EMT was inhibited. Meanwhile, mRNA and protein expressions of SIRT1 increased significantly(P<0.01), while mRNA expressions of TGF-β₁,Smad3,and Smad4 decreased(P<0.01). Under the intervention of SIRT1 blocker EX527, PNS could not play a significant inhibitory effect on the cells. Conclusion:PNS can prevent the occurrence of EMT of renal tubular epithelial cells induced by TGF-β₁, and the mechanism may be related to active SIRT1 to inhibit TGF-β₁/Smad pathway.

Objective To determine the prevalence of overweight and obesity among children and adolescents in Shenyang and the relationship between the birth weight to overweight and obesity in the childhood aged 7 to 17 years.Methods A stratified cluster representative sample of 5800 children and adolescents aged 7 to 17 years from 27 schools among 3 regions in Shenyang was selected.The study was carried out by using questionnaire about birth weight and physical examination including weight and height.Overweight and obesity were defined according to body mass index cutpoint.The body mass index classification criteria recommended by the Chinese Working Group on Obesity for Children(WGOC) was adopted,and the relationship between birth weight and overweight or obesity in children and adolescents were analyzed by collecting the information associated with overweight or obesity.Results The overall combined prevalence of overweight and obesity was 21.13％ with obesity as 7.12％ based on the WGOC criteria,and children of 10 to 12 years old were high-risk population groups.The risk of a male becoming overweight or obesity was 2.01 times higher than the risk of a female.The prevalence of overweight and obesity in Shenyang were level Ⅱ,especially for males the incidence of overweight and obesity of high birth weight babies was 1.73 (95％ CI:1.51-2.87) times higher than that of normal birth weight babies,while the incidences of overweight and obesity between low birth weight babies and normal birth weight babies were not statistically different.Conclusions The study indicated that the prevalence of overweight or obesity children and adolescents are level Ⅱ in Shenyang.There is high risk to be overweight and obesity in adolescences and especially for males.To decrease overweight and obesity in children and adolescents we should pay greater attention to fetus period.

Objective To explore the associations of body mass index (BMI), waist circumference (WC), waist height ratio (WHtR) and the prevalence of hypertension in elderly residents over 60 years in Baodi district, Tianjin. Methods Residents over 60 who underwent medical examinations in the Koudong Health Center, Baodi district, Tianjin, were all invited to participate in the study from April to May, 2018. Participants were asked to fill out structured questionnaires and undergo physical examinations. Stratified analysis and logistic regression analysis were applied to examine joint effects and interactions of BMI and WC (or WHtR) on the risk of hypertension. Results A total of 1 417 residents (83.75%) out of 1 692 residents participated in the study. The prevalence of hypertension in the participants was 46.36%. 66.50% of the participants were BMI overweight or obese. Participants with central obesity accounted for 74.66% (measured by the WC) and 75.38% (by the WHtR). Compared to the normal weight measured by the BMI or the WC, BMI overweight (OR=1.65, 95%CI: 1.19-2.30) or obesity (OR=3.41, 95%CI: 2.23-5.20) and WC central obesity (OR: 1.49, 95%CI: 1.00-2.23) were associated with increased risk of hypertension. The joint effects of BMI and WC (OR=2.49, 95%CI: 1.78-3.46), or BMI and WHtR (WHtR overweight: OR=2.05, 95%CI: 1.41-2.99; WHtR obesity: OR=2.37, 95%CI: 1.50-3.76) were greater than the single effect of the latter (WC overweight/obesity: OR=1.39, 95%CI: 0.90-2.15; WHtR overweight: OR=1.02, 95%CI: 0.62-1.66; WHtR obesity: OR=1.44, 95%CI：0.55-3.81). Conclusions Of the three indices, BMI is strongly correlated with the risk of hypertension. BMI overweight or obesity has enhanced the association of WC or WHtR and the risk of hypertension, suggesting that weight control in the normal range, especially measured by the BMI index, may prevent and control hypertension.

Diabetes Mellitus, Type 1 ; Humans ; Infant, Newborn ; Infant, Newborn, Diseases ; Insulin ; Mutation