OBJECTIVE: To establish an HPLC method for the determination of diprophylline in diprophylline tablets. METHODS: The analytical column was C18 and the mobile phase consisted of methanol-water (30∶ 70) at a flow rate of 1.0 mL?min-1. The detection wavelength was 273 nm and the sample size was 20 ?L. RESULTS: The linear range of diprophylline was 8.1～81 ?g?mL-1 (r=0.999 8) with an average recovery rate of 100.01% (RSD=0.21%). CONCLUSION: The method is simple, accurate and precise, and it can be used for the quality control of diprophylline tablets.

Objective To investigate the application effect of indwelling needle puncture in the axillary vein of newborn infants.Methods From September 2015 to February 2016,the venous indwelling needle puncture was given for neonatal transfusion 180 cases,including 90 cases of the axillary vein indwelling needle infusion in the experimental group,and 90 cases of puncture of extremity vein infusion in the control group.The application effect of the 2 groups was compared.Results There were significant differences in terms of indwelling needle shedding,phlebitis,drug extravasation,lien time between the experimental group and the control group (P ＜ 0.05) Conclusions During neonatal indwelling needle puncture infusion process,the axillary vein indwelling needle infusion can prevent the indwelling needle shedding,decrease the incidence of phlebitis and reduce the incidence of drug extravasation,and can prolong the indwelling time.Puncture in the simple operation easy,is easy to learn and no need for special facilities and equipment,only conventional intravenous infusion required material,plus 24g indwelling needle,aseptic transparent dressing,paper tape,economical and practical,suitable for application in neonatal ward.

Objective To investigate the application effect of the puncture method of indwelling needle by single hand in clinical attendance of pediatrics. Methods From March 2015 to September 2015,578 patients in our pediatrics were randomly and equally divided into two groups. The experiment group were treated with the puncture method of indwelling needle by single hand and the control group received traditional puncturing method. Results There were statistical significances in the success rate of first puncture,degree of comfort,sense of pain,dropping of the indwelling needle,phlebitis,exosmosis of drugs and indwelling time of patients between the two groups(P< 0.05). Conclusion In the process of using indwelling needle in pediatrics,the application of the puncture method of indwelling needle by single hand could improve the success rate of first puncture of venous retention needle,enhance the comfort degree of neonates,relieve the sense of pain,reduce the dropping of the indwelling needle,incidence of phlebitis and exosmosis of drugs,and prolong the indwelling time. The operation of this puncture is simple and easy to do and to learn,without any special equipment. In the venous puncture,the only necessities are 24G retention needle,3M indwelling needle and adhesive tape. Obviously,it is economical and practical,and is suitable for application in clinic.

The development of anti-epidermal growth factor receptor monoclonal antibodies (anti-EGFR McAbs) marked a significant milestone in metastatic colorectal cancer (mCRC) treatment. The addition of anti-EGFR McAb can greatly improve quality of life of mCRC patients and mCRC prognosis and markedly increases the overall survival rate from 6 months to nearly 30 months. KRAS and NRAS mutations contribute to the primary resistance to anti-EGFR therapy and can serve as well-established predictive markers for pa-tient selection. Apart from the RAS family, other molecular alteration in EGFR signaling pathway may also compromise the efficacy of anti-EGFR treatment. In addition, patients who responded to anti-EGFR treatment eventually develop acquired drug resistance within 13 and 18 months. In this review, the mechanisms underlying the primary and secondary resistance to anti-EGFR therapy are summa-rized, and a possible strategy to circumvent drug resistance is proposed. We hope this review can provide compelling evidence, deeper insights, and reasonable guidance to facilitate the precise molecular targeted therapy of mCRC.

Objective To introduce a new purchase management model of hospital.Methods Through a narration and a deep analysis on current status of hospital purchase,some disadvantages were summed up and new suggestions and solutions were put forward so as to reform purchase model.According to the characteristics of military hospitals,commercial bribery in national medical industry was eliminated and medical costs were reduced.The broad masses of ordinary people could benefit from it.Results The facts of the new purchase management model of hospital were clarified and analyzed in an unique perspective,which were proved to be worthy of researching.Conclusion More detailed work should be done to implement the model.

Factors that may influence vitamin D nutritional status include latitude,season,age,sex,race,skin color,clothing,cultural practices,dietary nutrition,and living conditions.This article reviews the effects of these factors on vitamin D status and recommends that any high-risk people of vitamin D deficiency should take vitamin D supplementation.

Objective To evaluated the sexual psychology changes and marriage quality for ovarian cancer patients after operation and supply selective psychological nursing intervention for patients and their family members.Methods Self-designed questionnaire was used to investigated ovarian cancer patients under 60 years old one month after operation to understand the sexual psychology changes and its related factors.Nursing intervention was adopted according to different conditions of patients.Results Factors that influenced the sexual psychology of patients included:age,education degree,chemotherapy process,clinical symptom,anxiety and depression status,marital status and degree of nursing intervention.Conclusions Sexual psychology Was influenced by many factors.We should adopt various kinds of nursing intervention to promote the physical and mental health of patients,facihtate the recovery of sexual function and improve their life quality.

Afferent connections to the nucleus tegmenti dorsalis were studied by means of retrograde axonal transport method of horseradish peroxidase (HRP). HRP was depositted stereotaxically via three approaches: vertical, oblique through the cerebellum or oblique through the cerebrum. The serial sections were treated by 3,3'-diamino-benzidine, benzidine dihydrochloride, diaminobenzidine-cobalt dichloride or tetrame-thylbenzidine respectively.Labeled neurons were observed in following nuclei: nucleus lateralis habenulae,nucleus interpeduncularis, nucleus mammillaris lateralis, nucleus posterior hypothalami, nucleus lateralis hypothalami, substantia grisea centralis mesencephali, nucleus dorsalis raphes, nucleus tractus solitarii, nucleus prepositus hypoglossi, nucleus parafascicularis and nucleus of Darkschewitsch.Then HRP was introduced into nucleus parafascicularis or nucleus prepositus hypoglossi, labeled terminals were also observed within the nucleus tegmenti dorselis.

Objective To synthesize a marine natural product, 24-methylenecholesterol. Methods The target molecule was synthesized in 7 steps using hyodeoxycholic acid as the starting material, 3?-(tetrahydropyran-2-yloxy)-cholest-5-ene-24-ketone as the key intermediate and isopropylation as the key step. Results The target molecule was synthesized with a total yield of 66% and confirmed by MS, IR, 1HNMR and 13CNMR. Conclusion Our synthesis of 24-methylenecholesterol is characterized by easily acquired starting material, short synthetic route, high yield and atom-economic.