OBJECTIVE:To establish RP-HPLC method for the determination of berberine hydrochloride in rabbit plasma.METHODS:RP-HPLC internal standard method was adopted in which Dikma diamonsil TM C 18 was used as chro-matographic column,the mobile phase was composed of acetonitrile-0.1mol/L potassium dihydrogen phosphate solution-sodium dodecyl sulphate(50∶50∶0.06)with flow rate at1ml/min,and the detection wavelength was345nm,the column temperature was40℃with paradimethylaminobenzal dehyde taken as the internal standard.RESULTS:Good linear relation-ship was achieved when the detection concentration of berberine hydrochloride was within the range of20.5～656ng/ml(r=0.9999),the average recovery rate was99.14%,and the mean RSD was3.78%.CONCLUSION:The method has high sen-sitivity and strong specificity,which can be used for the study of pharmacokinetic of berberine hydrochloride.

OBJECTIVE To observe the effect of nasal and oral disinfection with iodophor on lower respiratory tract infection after nasotracheal intubations.METHODS Totally 360 patients receiving trachea intubation under anesthesia were divided into three groups as follows: oral intubation group,nasal intubation group and nasal disinfection group pretreated with iodophor.Postoperative lower respiratory tract infection was investigated respectively.RESULTS The incidence of lower respiratory tract infection was higher in nasal intubation group(4.17%) and significantly higher than in oral intubation group(1.67%)(P

OBJECTIVE To observe the effect of levofloxacin used by intratracheal administration on lower respiratory tract infection after general anesthesia.METHODS Totally 300 patients receiving general anesthesia were divided into test group(n=150)and control group(n=150).Levofloxacin was used intratracheally twice during general anesthesia in test group.Postoperative lower respiratory tract infection was investigated.RESULTS The incidence of lower respiratory tract infection in control group(4.67%,n=7) was higher than in test group(0.67%,n=1) significantly(P

0.05 ) . For diagnosis of intracranial aneurysms by VR , sensitivity , specificity, accuracy, positive predictive value and negative predictive value were 96.8%, 92.9%, 95.9%, 97.8% and 89.7% respectively,while sensitivity , specificity, accuracy, positive predictive value and negative predictive value of DSA was 97.9%, 96.3%, 97.5%, 98.9%, 92.9% respectively. Of all 94 aneurysms, 3D spacial relationship of neck, body and parent artery of aneurysms could well demonstrated by VR in 89,while by DSA only in 57(60.6%). Conclusion MS 3D-CTA VR is a very quick ,economical and effective method in diagnosis of intracranial aneurysms and has very high sensitivity , specificity and accuracy.

Animals ; Chronic Disease ; drug therapy ; Drug Combinations ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; International Cooperation ; Medicine, Chinese Traditional ; trends ; Primary Prevention ; methods

Humans ; Laryngeal Neoplasms ; surgery ; Laryngectomy ; standards ; Reference Standards

Objective To summarize imaging features of Kallmann syndrome (KS) in children and to improve diagnostic level of the disease.Methods The imaging manifestations of 13 patients with clinically proved KS were retrospectively studied.MRI of rhinencephalon and left wrist X-ray examinations were performed in all the 13 children.Ultrasound of abdomen and pelvis was implemented in the 8 of 13 patients.Results All the patients had abnormalities of olfactory system.Bilateral olfactory bulbs and tracts were absent in 9 patients.Unilateral olfactory bulbs and tracts were absent in 4 patients,in which contralateral olfactory bulbs and tracts were hypoplastic.These patients presented 2 aplastic,17 hypoplastic and 7 normal olfactory sulci.Anterior pituitary was hypoplastic in 3 patients.Bone age showed retardation in 6 of 13 patients.Bilateral testicles were small in all the 8 cases by ultrasound.Conclusions Aplastic or hypoplastic olfactory bulbs and tracts and sulci are seen in children with KS.Anterior pituitary dysplasia is present in part of patients and bone age backward happens in approximate half of the children.

Adult ; Dust ; Female ; Glass ; Humans ; Male ; Middle Aged ; Occupational Diseases ; epidemiology ; Prevalence ; Skin Diseases ; epidemiology ; Young Adult

Objective:To evaluate the clinical efficacy and safety of repaglinide and gliclazide in the treatment of elderly type 2 di-abetes mellitus (T2DM). Methods:A total of 225 T2DM patients with the age over 60 were enrolled and randomly divided into repa-glinide group (n=112) and gliclazide group (n=113). The repaglinide group was given repaglinide 0. 5~2 mg, po, tid, while the gliclazide group was given gliclazide modified release tablets 30~120 mg, po, qd,both with the treatment course of 12 weeks. The level of FPG, 2h PG, HbAlC, TC and TG and the side effects were observed and the clinical efficacy was compared between the two groups. Results:The level of FPG, 2h PG, HbAlc and TC in the two groups showed significant decrease after the treatment (P<0. 01 or P<0. 05), and the level of 2h PG was decreased more significantly in the repaglinide group (P<0. 05). There were no significant differ-ences in the total effective rate (91. 8% vs 86. 2%), hypoglycemia incidence (4. 5% vs 8. 2%) and ADR incidence (6. 4% vs 10. 1%) in the two groups (P>0. 05). Conclusion:Both repaglinide and gliclazide show safe and reliable efficacy in the treatment of elderly T2DM.

Adjuvants, Immunologic ; therapeutic use ; Astragalus Plant ; Child ; Child, Preschool ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Infant ; Male ; Nucleic Acids ; therapeutic use ; Phytotherapy ; Pneumonia ; drug therapy ; immunology ; Recurrence ; Respiratory Tract Infections ; drug therapy ; immunology ; Tyrosine ; therapeutic use