OBJECTIVE:To establish RP-HPLC method for the determination of berberine hydrochloride in rabbit plasma.METHODS:RP-HPLC internal standard method was adopted in which Dikma diamonsil TM C 18 was used as chro-matographic column,the mobile phase was composed of acetonitrile-0.1mol/L potassium dihydrogen phosphate solution-sodium dodecyl sulphate(50∶50∶0.06)with flow rate at1ml/min,and the detection wavelength was345nm,the column temperature was40℃with paradimethylaminobenzal dehyde taken as the internal standard.RESULTS:Good linear relation-ship was achieved when the detection concentration of berberine hydrochloride was within the range of20.5～656ng/ml(r=0.9999),the average recovery rate was99.14%,and the mean RSD was3.78%.CONCLUSION:The method has high sen-sitivity and strong specificity,which can be used for the study of pharmacokinetic of berberine hydrochloride.

0.05 ) . For diagnosis of intracranial aneurysms by VR , sensitivity , specificity, accuracy, positive predictive value and negative predictive value were 96.8%, 92.9%, 95.9%, 97.8% and 89.7% respectively,while sensitivity , specificity, accuracy, positive predictive value and negative predictive value of DSA was 97.9%, 96.3%, 97.5%, 98.9%, 92.9% respectively. Of all 94 aneurysms, 3D spacial relationship of neck, body and parent artery of aneurysms could well demonstrated by VR in 89,while by DSA only in 57(60.6%). Conclusion MS 3D-CTA VR is a very quick ,economical and effective method in diagnosis of intracranial aneurysms and has very high sensitivity , specificity and accuracy.

OBJECTIVE To observe the effect of nasal and oral disinfection with iodophor on lower respiratory tract infection after nasotracheal intubations.METHODS Totally 360 patients receiving trachea intubation under anesthesia were divided into three groups as follows: oral intubation group,nasal intubation group and nasal disinfection group pretreated with iodophor.Postoperative lower respiratory tract infection was investigated respectively.RESULTS The incidence of lower respiratory tract infection was higher in nasal intubation group(4.17%) and significantly higher than in oral intubation group(1.67%)(P

OBJECTIVE To observe the effect of levofloxacin used by intratracheal administration on lower respiratory tract infection after general anesthesia.METHODS Totally 300 patients receiving general anesthesia were divided into test group(n=150)and control group(n=150).Levofloxacin was used intratracheally twice during general anesthesia in test group.Postoperative lower respiratory tract infection was investigated.RESULTS The incidence of lower respiratory tract infection in control group(4.67%,n=7) was higher than in test group(0.67%,n=1) significantly(P

Animals ; Chronic Disease ; drug therapy ; Drug Combinations ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; International Cooperation ; Medicine, Chinese Traditional ; trends ; Primary Prevention ; methods

Humans ; Laryngeal Neoplasms ; surgery ; Laryngectomy ; standards ; Reference Standards

Objective To summarize imaging features of Kallmann syndrome (KS) in children and to improve diagnostic level of the disease.Methods The imaging manifestations of 13 patients with clinically proved KS were retrospectively studied.MRI of rhinencephalon and left wrist X-ray examinations were performed in all the 13 children.Ultrasound of abdomen and pelvis was implemented in the 8 of 13 patients.Results All the patients had abnormalities of olfactory system.Bilateral olfactory bulbs and tracts were absent in 9 patients.Unilateral olfactory bulbs and tracts were absent in 4 patients,in which contralateral olfactory bulbs and tracts were hypoplastic.These patients presented 2 aplastic,17 hypoplastic and 7 normal olfactory sulci.Anterior pituitary was hypoplastic in 3 patients.Bone age showed retardation in 6 of 13 patients.Bilateral testicles were small in all the 8 cases by ultrasound.Conclusions Aplastic or hypoplastic olfactory bulbs and tracts and sulci are seen in children with KS.Anterior pituitary dysplasia is present in part of patients and bone age backward happens in approximate half of the children.

Adult ; Dust ; Female ; Glass ; Humans ; Male ; Middle Aged ; Occupational Diseases ; epidemiology ; Prevalence ; Skin Diseases ; epidemiology ; Young Adult

Objective To analyze the relationship between carotid intima-media thickness (CIMT) and metabolic syn?drome (MS), and explore risk factors for CIMT incrassation in Tianjin policemen. Methods Fifty MS patients (MS group) and 50 healthy people (control group) in health examination of Tianjin policemen were enrolled in this study. The question?naire survey(family history,past medical history,present history,living habits and occupational stress factors) were recorded in two groups of patients. The blood indicators (fasting plasma glucose, triglycerides, total cholesterol, high density lipopro?tein cholesterol, low density lipoprotein cholesterol, homocysteine) were detected. The color Doppler was used to measure CIMT incrassation, and incrassation ratio was calculated. The relative indicators were compared between two groups. The lo?gistic regression model was used to analyse the influencing factors of carotid artery intima-media thickness. Results CIMT and CIMT incrassation ratio (communis, internal and external carotid) were significantly higher in MS group than those of control group (P<0.01). The risk factors for CIMT included family history of hypertension, chronic stress, smoking, family history of diabetes, high fat diet and high homocysteine levels, and dairy products were protective factors for CIMT incrassa?tion. Conclusion The reason of carotid artery intima-media thickness is diversity. The healthy lifestyle and clinical inter?vention to retard CIMT are advocate, which are conducive to the control of atherosclerosis.

Objective To explore the diagnostic value of diffusion weighted imaging (DWI) and CT perfusion imaging (CTPI) in diagnosis of liver fibrosis. Methods Fifty-seven hepatic fibrosis patients and 23 normal controls received DWI (b=500 s/mm2), ADC value of different fibrosis stages was measured, while 35 patients and all 23 normal controls received CTPI. The parameters of CTPI including blood flow (BF), blood volume (BV), mean transit time (MTT), hepatic arterial fraction (HAF) and permeability surface (PS) were measured. Analysis of variances was performed to compare the difference among the groups in both examinations. ROC curve was used to analyze the sensitivity and specificity of DWI and CTPI. Results The difference of ADC value between control group and S1 group was not significant, but between control group and S2, S3, S4 group and among group S2, group S3, group S4 was significant. In the parameters of CTPI, only the difference of HAF between control group and S3-S4 group was significant. Sensitivity and specificity of DWI and CTPI was 78.90%, 82.60% and 66.67%, 73.91%, respectively. Conclusion DWI is superior to CTPI for early diagnosis and degrading of liver fibrosis.