Objective:to study perspective of college students on counseling and psychotherapy Method:440 college students were asked to fill up a mental health knowledge questionnaire made by the authors Result:most of college students attached importance to and had a positive attitude towards counseling and psychotherapy, however they misunderstood some concepts and were not satisfied with the service available Conclusion:counseling and psychotherapy are in the early development stage in China

Objective:To investigate college students' perspective of mental health Method:440 college students were asked to fill up SCL-90 and a mental health knowledge investigation questionnaire made by the authors Result:Most students concerned about their mental health and liked to learn the knowledge of mental health However, there were still some aspects they kept incorrect ideas The results also showed that there were some difference in the attitude toward mental health, as well as the comprehension of the knowledge of mental health between male and female; among freshman, sophomore, junior and senior; between students in different universities, and between students who had visited counselors or psychotherapists and others who had not Conclusion:Pearson correlation analysis showed that college students' knowledge of and attitude to mental health related to their status of mental heath significantly

Retinoblastoma is the most common intraoeular malignant tumor in children.In its treatment,drug therapy is one of the most effective and irreplaceable methods.To reduce the toxicity of drugs,enhance the therapeutic effect and lessen drug resistance, some new drug deliver systems and new medicines for retinoblastoma are coming into being.Ophthalmic arterial infusion,fibrin sealant and iontophoresis are newly-found drug deliver systems.And the latest drugs under research include nutlins,phenoxazine derivative Phx-1,combretastatin A4 phosphate,2-deoxy-d-glucose and histone deacetylase inhibitors,etc.The following text is focused on the two aspects of the drug therapy for retinoblastoma.

Objective To discuss the influences of programmed death-1 (PD-1) factor on osteosarcoma cells MG-63.Methods Osteosarcoma stem cells were sovted and identified through osteosarcoma cell strain MG-63 cells.The influence of PD-1 signal on T cells proliferation were detected by MTT. The expression of PD-1 mRNA was detected by RT-PCR.Results Cancer cells had an obvious proliferation within one week, which showed the strong ability of proliferation and aggressivity.The formation of tumor cells spheres depended on the support of serum nutrition.The number of MG-63 cells proliferation in serum culture medium was significantly higher than the osteosarcoma cells spheres in serum-free suspension culture ( P<0.05 ) .Pluripotent stem cell marks that the expression of CD133 in cancer cell sphere was significantly higher than that of MG-63(P<0.05).RT-PCR results showed the PD-1 expression level of cancer cell sphere and MG-63 were increased significantly.Conclusion MG-63 cell line has the character istics of osteosarcoma stem cells.MG-63 cell line can express the corresponding cell markers.The expression of PD-1 also increase significantly which can reduce immune function of patients and is closely related with the occurrence and development of tumors.

Embryonic Stem Cells ; Humans ; Myocardial Infarction ; therapy ; Stem Cell Transplantation

Rabbit's brain phospholipids (RBP) 100~200 mg ?kg-1 ip or sc showed anti-inflammatory action on four kind of acute inflammatory animal models. Cephalin and Lecithin were the effective component of antiin-flammatory action on the acute inflammatory animal model. But RBP did not show antiinflam-matory action on the chronic proliferative inflammatory model. RBP increased blood carbon particle clearance, perfaneal macrophage phagocytosis and enhanced lymphocyte proliferation,hemolysin antibody in normal and immunode-pression animal. RBP increased the cGMP content in the liver tissue of immunodepression animal, 0. 1~1 mg ?L-1 RBP promoted proliferation but 100 mg ?L-1 RBP inhibited proliferation induced by PHA in vitro on human lymphocyte.

Objective:To screen the best decocting technology by multi-index comprehensive score combined with response sur-face. Methods:The content of paeoniflroin and the ratio of dry extraction were used as the indicators, and response surface Central Composite with two factors and five levels was used in the experimental design. The effects of the ratio of solvent to solid, decocting du-ration and decocting times on the preparation process of tonic semifluid extract of ten ingredients were studied. Results:Design Expert 8. 0. 6b software was used for the data analysis. A quadratic mathematical model between the factors and the comprehensive score of paeoniflroin content and the ratio of dry extraction was established. Combined with the actual production, the best decocting conditions were as following:the ratio of solvent to solid was 10. 6 ml·g-1 , the decocting duration was 96 min, and decocted 3 times. Conclu-sion:The optimal decocting technology is scientific, reasonable and stable.

Objective To investigate the value of 99Tcm-MDP imaging for the differential diagnosis between infection and aseptic loosening after total hip arthroplasty.Methods During February 2008 to August 2011,74 patients (32 males,42 females,average age (64.3±11.2) years) with hip pain after arthroplasty underwent 3-phase (blood flow,blood pool and bone phases) 99Tcm-MDP imaging.All patients had measurements of serum C reactive protein (CRP) and erythrocyte sedimentation rate (ESR).Joint infection was defined as either increased peri-prosthetic soft tissue activity during perfusion and blood pool phases or increased peri-prosthetic bone activity during uptake phase,or positive in all 3 phases.Aseptic loosening was defined as having negative 99Tcm-MDP in all 3 phases.Clinical diagnosis was chosen as the gold standard.The blood flow-pool imaging was compared with the serum examinations.x2 test was used for statistical analysis.Results There were 74 symptomatic joints in 74 patients,including 24 joint infections and 50 aseptic loosening.For the detection of peri-prosthetic infection,combined perfusion-blood-pool phase was more accurate than bone uptake phase (90.5％ (67/74) vs 55.4％ (41/74) ;x2=23.159,P＜0.001),with the sensitivity of 91.7％ (22/24)vs 70.8％ (17/24) and specificity of 90.0％ (45/50) vs 48.0％ (24/50),respectively.The blood flow-pool imaging was also more accurate than CRP (73.0％ (54/74) ;x2 =7.656,P＜0.05) and ESR (71.6％ (53/74) ; x2 =8.633,P＜0.05),respectively.Conclusion 99Tcm-MDP perfusion/blood flow-pool imaging is an accurate modality for differentiating peri-prosthetic infection from aseptic loosening in patients with hip pain after arthroplasty.

Objective To explore the relationship between the carotid artery atherosclerotic(CAA) plaque and its risk factors and cerebral infarction(CI).Methods 196 patients with CI(CI group) and 104 patients without CI(non-CI group) were examined by color Doppler ultrasonography on their carotid artery.The relationships were analysed between the CAA plaque with age,sex,accompany disease,smoking and alcohol drinking of all the pataints.Also,the distribution of different grade plaque was observed in each group.Results The incidences of CAA plaque were higher in patients with aged,accompainied hypertension,diabetes,abnormal blood-lipids and smoking(P

Objective To evaluate the efficacy of laparoscopic-assisted resection of the small bowel for stromal tumors.Methods From January 2003 to May 2007,laparoscopic abdominal exploration was carried out under general anesthesia on 10 patients with space occupying lesion and 5 patients with abdominal pain and hemafecia of unknown origin.After an intestinal lesion was found,a small incision was made at the proper site on the abdominal wall according to the location of the lesion.The diseased intestine was resected outside the abdominal cavity,and then end-to-end anastomosis was performed.Pneumoperitoneum was rebuilt after closing the abdominal cavity in order to observe the blood circulation of the small bowel and intra-abdominal hemorrhage.Results The operation was completed in all the 15 cases.The postoperative pathological examination showed stromal tumors with CD117 positive in 14 cases(93%)and CD34 positive in 9(60%).Three(20%)of the patients were at very low risk,5(33%)at low risk,4(27%)at moderate risk,and 3(20%)at high risk.The mean diameter of the tumors was(2.44?0.63)cm(ranged from 1.5 to 3.6).The operation time was 38 to 72 minutes with a mean of(57.8?10.4),and mean blood loss was(20.1?6.5)ml(10 to 30).The patients were discharged 4 to 7 days after the operation [mean,(5.3?1.1)days],and were followed up for 5 to 36 months [mean,(24.3?8.4)months].No patient had postoperative complications or recurrence in this series.Conclusion Laparoscopy is effective for resection of small bowel stromal tumors.