Recently,studies have demonstrated that several signaling pathways including Wnt,Notch,and Hedgehog which are involved in the regulation of the stem cells are abnormally activated in lung cancer.They are closely associated with some properties of the lung cancer stem cells,such as high tumorigenic,high metastasis,drug resistance and so on.In addition,several studies have shown that the population of the lung cancer stem cells,which are resistant to radiotherapy and chemotherapy significantly,highly express drug resistance proteins.Therefore,how to target lung cancer stem cells and ultimately cure the disease is becoming a hotspot in the cancer targeted therapy.

Objective To evaluate the effect of a new schistosomiasis control strategy based on the infection source control in four-lake regions of Hubei Province. Methods The new strategy based on the infection source control included the safe water sup-ply,feces harmless treatment,replacing cattle with machine,captive livestock,chemotherapy,Oncomelania hupensis snail con-trol in susceptible areas,hardening ditches,etc,and the new control strategy was implemented for 7 years in 9 counties(cities or districts)of the four-lake regions. The schistosomiasis situations were investigated before and after the intervention of the new strat-egy. Results The coverage rates of feces harmless toilets(three format toilets),biogas digesters,and safe water supply were 42.00%,23.16% and 93.76%,respectively. The number of ditch hardening was 1 960;the area of eliminating snails was 1 378.42 hm2;the number of persons who received the health education was 3 524 818 accounting for 92.17%of the total popula-tion;the number of person-time of taking active protection measures was 516 636 963. The average annual decline rates of schisto-some infection were 24.42%and 38.38%in residents and cattle,respectively. The endemic villages decreased by 4.77%,the vil-lages with snails decreased by 7.05%,and the villages with snails accounted between 63.19%and 66.21%of the total endemic vil-lages. The area with snails decreased by 90.35%,and the advance schistosomiasis patients decreased by 5.55%,and the fatality rate of advanced schistosomiasis patients decreased by 1.24%. No acute schistosomiasis patients occurred. Type One and Two en-demic(heavy endemic)villages decreased to zero;Type Three endemic(moderate endemic)villages decreased by 10.22%;but Type Four endemic(mild endemic)villages increased by 66.38%;and Type Five endemic(no endemic for 5 years)villages de-creased by 0.22%. The standards of schistosomiasis transmission controlled have achieved on schedule. Conclusion The imple-mentation of the new schistosomiasis control strategy based on the infection source control in four-lake regions of Hubei Province can effectively control schistosomiasis.

To evaluate the items of critical values and alert limits of the test results , to optimize the critical values report procedure , to modify the laboratory information system ( LIS ) and the hospital information system ( HIS ) , the critical values monitoring platform was designed .Through the monitoring platform,the critical value report rate and critical value report timely rate could be calculated , so reduce medical risks and improve the level of hospital management .

Humans ; Humidity ; adverse effects ; standards ; Incubators, Infant ; Infant, Newborn ; Intensive Care Units, Neonatal ; Water Supply ; standards

Child ; Hemofiltration ; Humans ; Male ; Paraquat ; poisoning ; Poisoning ; therapy ; Treatment Outcome

Effect of S83143, a praziquantel derivative, and its phosphate salt S85009, sulfate salt S85010 and hydrochloride salt S85021 on different developmental stages of Schistosoma japonicum in mice were ob- served. When infected mice were treated with S83143 suspension at a single oral dose of 400 mg/kg or with 3 different salts of S83143 at a single intramuscular dose of 100 mg' ( S83143 base )/kg, the worm reduction rate was 69~87% in 2 h old schistosomula and 47~96% in 32~33 d old adult worms, respectively, but had no effect on 3 ~14 d schistosomula. The results showed that the susceptibility of different developmental stages of schistosome to S83143 was similar to that of praziquantel.The acute LD50 of ig S83143 suspension was more than 10g/kg, while ED50 was 352?65 mg/kg. With im S85010 solution, the LD50 and ED50 were 176?14 and 66?10 mg/kg respectively. Therefore, the therapeutic index of S83143 was over 28, which was higher than 2.67 of S8S010.

Objective To explore the distribution of high-arsenic water in Shaanxi Province in order to provide theoretical basis for the control of arsenism.Methods The contents of arsenic of 4042 water sampled from 1898 nature villages in 12 counties were assessed using semi-quantitative reagent-knit,and the water samples of Shanyang County,the targeted area,was quantified using quantitative atomic fluorescent speemetry.Results The contents of arsenic of 6 water samples in 2 villages exceeded 0.05 mg/L,accounting for 0.15%(6/4042),were 0.46,0.53,0.63,0.69,0.74,1.53 mg/L,respestively,and located in Shanyang County.A crowd of 1146 exposed to hish asenic water.among whom 555 were children.Conclusions Arsenic in drink water in Shanyang County in Shaanxi Province is higher than the standard,to which a large number of people are exposed,water-improving and defluoridotion must be carried out as soon as possible,reduces harm of high arsenic.

Objective To study immunologic mechanism of tiger's urine on patients of rheumatoid arthritis. Method We used ELISA method to detect TNF values of serum; we also used MTT colorimetric method to detect the inhibition of tiger's urine on T－ cell. Result TNF values of RA patient's serums at both active stage and stationary phase before the therapy were higher than those of healthy group; TNF values of patient's serums after the therapy were lower than those before the therapy; tiger's urine had obvious inhibitive action on proliferation of peripheral blood's T－ cell. Conclusion Tiger's urine could inhibit T－ cell's proliferation and TNF's secretion, which may be the important mechanism of curing RA.

Objective To detect the mutation of A379,we develop a TaqMan fluorogenic probe based amplification refractory mutation system (TaqMan-ARMS) and investigate whether the A379V variant and activity of Lp-PLA2 are the risk factors for CAD.Methods According to the amplification refractory mutation system(ARMS-PCR)combined with a TaqMan fluorogenic probe,we established and evaluated the assay teehnique of TaqMan-ARMS for measuring the genotype of variant.At the same time,we tested the aetivity of Lp-PLA2 in 395 patients with coronary heartdisease and 396 controls,whose clinical information [ages,CHO,GLU,TG,HDL,LDL,Hs-CRP,Lp (a)] were collected.Date was analyzed by using Independent-samples t test,Chi-square test,One-Way ANOVA,Binary Logistic Regression.Results CAD group had significantly higher Lp-PLA2 activity than the controls(31.51 nmol · ml-1 · min-1 ＞21.31 nmol ·ml-1 · min-1,F =16.40,P ＜ 0.001).Comparing the highest quartile of Lp-PLA2 activitv to the bottom quartile,OR was 7.50 (95％ CI:2.34-24.05) after adjustment for various traditional cardiovascular risk factors,including ages,sex,CHO,TG,Hs-CRP,Lp (a) and GLU; the genotype VV of A379V was associated with higher risk of CAD (OR =2.95; 95％ CI:1.22-7.15,P ＜ 0.05).Conclusions The TaqMan-ARMS real time PCR technique is established to analyze A379V genotype.Lp-PLA2 activity is significantly higher in CAD group and is a risk factor for CAD; the genotype VV of A379V is also a risk factor for CAD.

Objective To explore the effect of peritoneal dialysis to treat renal failure in children.Methods There were 11 admitted patients of renal failure in our department from July 2003 to April 2008.Their clinical data and follow-up results were analyzed.Results No patient was dead during treatment.The average time of peritoneal dialysis treatment in the children with acute renal failure was 15.5 days in hospital,which was 22.8 days in the chronic patients.After treatment the levels of serum nitrogen and creatine were decreased significantly from (34.03±8.44)mmol/L and (710.09±167.54)μmol/L to (15.94±4.93)mmol/L and (233.87±92.71)μmol/L (P＜0.01).The serum sodium and bicarbonate ion were increased from (130.91±9.15)mmol/L and (14.56±2.07)mmol/L to (139.46±3.98)mmol/L and (22.47±3.29)mmol/L (P＜0.05,P＜0.01).The duration of follow-up were from one month to 5 years.The level of serum nitrogen and creatine in 5 patients of acute renal failure were normal and the analysis of urine was also normal during follow-up.One patient had renal transplantation after peritoneal dialysis.Three patients still regularly underwent peritoneal dialysis.Conclusion The peritoneal dialysis combining with multi-modality treatment was the better style of renal replacement therapy in renal failure patients.