Objective To discuss the present situation of nearly five years of traditional Chinese medicine for the diagnosis and treatment of children with bronchial asthma,confirmed that the curative effect of traditional Chinese medicine for the treatment of the disease is reliable,thus to provide certain theoretical basis for further research in the future.

Objective To study the effect of baicalin on the expression of receptor activator of nuclear factor-κB ligand (RANKL) and osteoprotegerin (OPG) in cultured human periodontal ligament (HPDL) cells. Methods Small interfering RNA (siRNA) eukaryotic expression vector targeted transforming growth factor βⅡ receptor (TGF-β RⅡ) was constructed and transfected into T cells. HPDL cells with T cells transfected with siRNA or not were placed in the culture medium that had been added with lipopolysaccharide (LPS) and baicalin. The obtained solution was divided into six groups according to the components (group Ⅰ: HPDL cells+LPS+T cells transfected with siRNA1+baicalin;group Ⅱ: HPDL cells+LPS+T cells transfected with siRNA1; group Ⅲ: HPDL cells+LPS+T cells+baicalin; group Ⅳ: HPDL cells+LPS+T cells; group Ⅴ: HPDL cells+baicalin; group Ⅵ: HPDL cells) and was cultured for 48 hours. RT-PCR was used to observe the effect of baicalin on the expression of OPG-RANKL in HPDL cells. Results The ratio of RANKL/OPG in group Ⅰ was lower than that in group Ⅱ (P<0.01) and higher than that in group Ⅲ (P<0.01); The ratio of RANKL/OPG in gronp Ⅲ was lower than that in group Ⅳ (P<0.01); the ratio of RANKL/ OPG in group Ⅳ was higher than that in group Ⅵ (P<0.01); the ratio of RANKL/OPG in group Ⅴ was lower than TGF-β signaling transduction plays an important role in the effect of baicalin on the RANKL/OPG ratio in HPDL cells.

To investigate the changes of cardiac myosin ATPase and its isoenzyme in early stage of cardiac hypertrophy following myocardial infarction(MI), we studied myosin ATPase activitzy, isoenyme distriibution and myocardial fiber diameter of uninfarcted ventricu lar sfptum, 9 aed 21 days after operation in MI rats. The results showed that coincident with the increases in myocardial fiber diameter on 9 days after MI, both myosin ATPase activity and V_1 isoenzyme amount were depressed. These changes were more pronounced on 21 after MI. It is suggested that with the development of myocardial hypertrophy in early stage of MI, adaptive changes in myosin ATPase activity and isoenzyme distribution occur.

Rheumatoid arthritis (RA) is a chronic inflammatory disease characterized by polyarthritis.Numerous agents with varying mechanisms are used in the treatment of RA,but there is no radical cure.Interleukin-6 (IL-6) plays an important role in the pathogenesis of RA.Agents targeting IL-6 such as tocilizumab (TCZ) attracted significant attention as a promising agent in RA treatment.This article reviewed the key efficacy and safety data from clinical trials of TCZ,as well as six candidate IL-6 blockers including sarilumab,ALX-0061,sirukumab,MEDI5117,clazakizumab,and olokizumab,and their future perspectives in the treatment of RA.

Objective: To observe the expression changes of protein tyrosine phosphatase 1B (PTP1B) mRNA and protein during the differentiation of 3T3-L1 preadipocytes, so as to explore the relationship between PTP1B and adipocytes differentiation. Methods: 3T3-L1 preadipocytes were cultured in vitro and were induced to differentiate into mature adipocytes; the differentiation of adipocytes was assessed through detecting expression of peroxisome proliferator-activated receptor-gamma 2 (PPARγ2) mRNA by RT-PCR and oil red O staining. Expression of PTP1B in adipocytes was detected by RT-PCR and Western blot during differentiation. Results: With the progression of 3T3-L1 cell differentiation, oil red O staining showed that the lipid droplets increased gradually to 90% of the vision field; meanwhile, the expression of PPARγ2 also increased gradually, suggesting the proliferation and maturation of the preadipocytes. The expression of PTP1B mRNA and protein decreased in 3T3-L1 preadipocytes with their differentiation and maturation; the expression reached the lowest level in mature adipocytes. Conclusion: Expression of PTP1B mRNA and protein decreases during the differentiation of preadipocytes, indicating a role for PTP1B in the maturation of adipocytes.

Lung transplantation is an effective treatment for end-stage lung diseases in children, and the only treatment for children with no response to conservative treatment.However, merely a few transplantation centers at home and abroad can provide lung transplantation at present.The mortality of pediatric lung transplantation is high, and it is difficult for donor matching.Besides, it has many complications.In recent years, pediatric lung transplantation has made positive progress and technological breakthroughs in many aspects.In this article, research progress in pediatric lung transplantation at home and abroad was reviewed.

Regulatory T cells play an important role in regulating immune responses.Recently,a novel immune Treg cell with phenotype TCRαβ + CD3 + CD4-CD8-NK1.1-was known as the Double-negative T cells (DNT).DNT cells are able to suppress CD4 + and CD8 + T cells,B cells as well as NK cells and dendritic cells(DCs) and play an important role in regulating immune responses in models of autoimmunity,infection,inflammatory disease,transplantation and tumor immunity.Now DNT cells have already become a hot point in the research of the immunotherapy.This article mainly introduces the possible sources of DNT cells and the mechanisms of their functions.

There has been a growing number of prostate cancer patients in our country,and most of the patients suffer with bone metastases.Although more and more new drugs are reported for the treatment of prostate cancer,tumor local recurrence or metastasis occur within the 10 years after treatment.The diagnostic sensitivity of 18F-NaF PET/CT for bone metastases from prostate cancer is high.18F-NaF PET/CT not only can locate the lesion precisely,evaluate the drug efficacy quantitatively,but also can be used to follow up prostate cancer patients.This review is focused on the application of 18F-NaF PET/CT in bone metastases from prostate cancer.

We observed the effects of testosterone (T) on myosin ATPase activity and its isoenzyme distribution of noninfarcted myocardium in male infarcted rats. 21 days after myocardial infarction (MI), both myocardial fiber diameter and myosin ATPase activity were increased in T treated MI group when compared with that of the MI group (P

Objective To investigate the clinical efficacy of subsection partial posterior internal sphincterotomy and posterior in-ternal sphincterotomy for chronic anal fissure .Methods 64 cases with stage Ⅱ ,Ⅲ anal fissure were randomly divided into treat-ment group and control group ,treatment group of 31 cases of subsection partial posterior internal sphincterotomy ,33 patients in the control group after posterior internal sphincterotomy ,two groups of cases after 24 hours of operation ,complications ,curative effect of postoperative recurrence comparative analysis of rate ,healing time ,postoperative complications in 3 months .Results In the treatment group ,the cure rate was 90 .32% ,in the control group ,the cure rate was 90 .9% ,compared with no significant difference between the two groups ;the two groups had no recurrence in two cases ;24 hours after operation complications ,healing time ,post-operative complications in 3 months ,the difference was statistically significant (P<0 .05) .Conclusion subsection partial posterior internal sphincterotomy in treatment of chronic anal fissure with faster postoperative recovery and fewer complications .