Objective:to study perspective of college students on counseling and psychotherapy Method:440 college students were asked to fill up a mental health knowledge questionnaire made by the authors Result:most of college students attached importance to and had a positive attitude towards counseling and psychotherapy, however they misunderstood some concepts and were not satisfied with the service available Conclusion:counseling and psychotherapy are in the early development stage in China

Objective:To investigate college students' perspective of mental health Method:440 college students were asked to fill up SCL-90 and a mental health knowledge investigation questionnaire made by the authors Result:Most students concerned about their mental health and liked to learn the knowledge of mental health However, there were still some aspects they kept incorrect ideas The results also showed that there were some difference in the attitude toward mental health, as well as the comprehension of the knowledge of mental health between male and female; among freshman, sophomore, junior and senior; between students in different universities, and between students who had visited counselors or psychotherapists and others who had not Conclusion:Pearson correlation analysis showed that college students' knowledge of and attitude to mental health related to their status of mental heath significantly

Retinoblastoma is the most common intraoeular malignant tumor in children.In its treatment,drug therapy is one of the most effective and irreplaceable methods.To reduce the toxicity of drugs,enhance the therapeutic effect and lessen drug resistance, some new drug deliver systems and new medicines for retinoblastoma are coming into being.Ophthalmic arterial infusion,fibrin sealant and iontophoresis are newly-found drug deliver systems.And the latest drugs under research include nutlins,phenoxazine derivative Phx-1,combretastatin A4 phosphate,2-deoxy-d-glucose and histone deacetylase inhibitors,etc.The following text is focused on the two aspects of the drug therapy for retinoblastoma.

Objective To discuss the influences of programmed death-1 (PD-1) factor on osteosarcoma cells MG-63.Methods Osteosarcoma stem cells were sovted and identified through osteosarcoma cell strain MG-63 cells.The influence of PD-1 signal on T cells proliferation were detected by MTT. The expression of PD-1 mRNA was detected by RT-PCR.Results Cancer cells had an obvious proliferation within one week, which showed the strong ability of proliferation and aggressivity.The formation of tumor cells spheres depended on the support of serum nutrition.The number of MG-63 cells proliferation in serum culture medium was significantly higher than the osteosarcoma cells spheres in serum-free suspension culture ( P<0.05 ) .Pluripotent stem cell marks that the expression of CD133 in cancer cell sphere was significantly higher than that of MG-63(P<0.05).RT-PCR results showed the PD-1 expression level of cancer cell sphere and MG-63 were increased significantly.Conclusion MG-63 cell line has the character istics of osteosarcoma stem cells.MG-63 cell line can express the corresponding cell markers.The expression of PD-1 also increase significantly which can reduce immune function of patients and is closely related with the occurrence and development of tumors.

Embryonic Stem Cells ; Humans ; Myocardial Infarction ; therapy ; Stem Cell Transplantation

Objective To investigate the value of 99Tcm-MDP imaging for the differential diagnosis between infection and aseptic loosening after total hip arthroplasty.Methods During February 2008 to August 2011,74 patients (32 males,42 females,average age (64.3±11.2) years) with hip pain after arthroplasty underwent 3-phase (blood flow,blood pool and bone phases) 99Tcm-MDP imaging.All patients had measurements of serum C reactive protein (CRP) and erythrocyte sedimentation rate (ESR).Joint infection was defined as either increased peri-prosthetic soft tissue activity during perfusion and blood pool phases or increased peri-prosthetic bone activity during uptake phase,or positive in all 3 phases.Aseptic loosening was defined as having negative 99Tcm-MDP in all 3 phases.Clinical diagnosis was chosen as the gold standard.The blood flow-pool imaging was compared with the serum examinations.x2 test was used for statistical analysis.Results There were 74 symptomatic joints in 74 patients,including 24 joint infections and 50 aseptic loosening.For the detection of peri-prosthetic infection,combined perfusion-blood-pool phase was more accurate than bone uptake phase (90.5％ (67/74) vs 55.4％ (41/74) ;x2=23.159,P＜0.001),with the sensitivity of 91.7％ (22/24)vs 70.8％ (17/24) and specificity of 90.0％ (45/50) vs 48.0％ (24/50),respectively.The blood flow-pool imaging was also more accurate than CRP (73.0％ (54/74) ;x2 =7.656,P＜0.05) and ESR (71.6％ (53/74) ; x2 =8.633,P＜0.05),respectively.Conclusion 99Tcm-MDP perfusion/blood flow-pool imaging is an accurate modality for differentiating peri-prosthetic infection from aseptic loosening in patients with hip pain after arthroplasty.

0.01). Conclusion: Occlusal reduction might diminish the rate of postoperative pain following endodontic instrumentation.

Objective:To screen the best decocting technology by multi-index comprehensive score combined with response sur-face. Methods:The content of paeoniflroin and the ratio of dry extraction were used as the indicators, and response surface Central Composite with two factors and five levels was used in the experimental design. The effects of the ratio of solvent to solid, decocting du-ration and decocting times on the preparation process of tonic semifluid extract of ten ingredients were studied. Results:Design Expert 8. 0. 6b software was used for the data analysis. A quadratic mathematical model between the factors and the comprehensive score of paeoniflroin content and the ratio of dry extraction was established. Combined with the actual production, the best decocting conditions were as following:the ratio of solvent to solid was 10. 6 ml·g-1 , the decocting duration was 96 min, and decocted 3 times. Conclu-sion:The optimal decocting technology is scientific, reasonable and stable.

OBJECTIVE: To explore the diagnostic value of brain natriuretic peptide (BNP) level in patients with sepsis of blood-stasis syndrome. METHODS: The prospective method of clinical diagnostic test and evaluation principles of diagnostic test were applied. One hundred and seventy-four patients with sepsis were divided into two groups: blood-stasis syndrome group and non blood-stasis syndrome group. The levels of serum BNP in two groups were detected. RESULTS: The level of BNP in sepsis patients was related to blood-stasis syndrome (P<0.01). BNP cut-off level was 150 ng/L, sensitivity was 85.3%, specificity was 81%, the maximum value of Youden index was 0.663, and the area under the receiver operating characteristic curve (ROC) value was (0.886+/-0.025). CONCLUSION: BNP can be used as an objective index of blood-stasis syndrome diagnosis for sepsis, and BNP with boundary value of 150 ng/L is an optimal biological index.

Objective To investigate the expression and clinical significance of peroxisome proliferator activated receptor γ(PPARγ)in bladder urothelial cancer tissues.Methods Parafflin‐embeded specimen of bladder urothelial cancer tissues from 50 cases and normal tissues near the bladder urothelial cancer from 5 cases were harvested from the Pathology Department of the Renmin Hospital of Wuhan University between 2006 and 2009.Those cases had complete pathological and clinical data.The ex‐pression of PPARγ was detected by immunohistochemical SP method.Quantitative analysis of the PPARγ was measured by high definition pathological graphics context report system (HPIAS‐1000).One‐way analysis of variance and SNK (q)tests were used to analyze the mean density and the positive area rate of the immunohistochemical results.All data were processed by SPSS 13.0.Results The expression of PPARγwas significantly higher in bladder urothelial cancer tissues than in para‐carcinoma tis‐sues(P<0.05).Correlation between expression of PPARγ with TNM stag of bladder urothelial cancer was as follows :Positive rate of PPARγin the tissues with primary tumor size ≥3 cm was 72.4% ,significantly higher than 33.3% in the tissues with tumor size <3 cm(P<0.05);positive rate of PPARγin the cases with lymph node metastasis was 72.7% ,significantly higher than 46.4% in the cases without lymph node metastasis(P<0.05);positive rate of PPARγin patients at stage T3‐4 group was 75.0% ,significantly higher than 41.9% and 45.5% in patients at clinical stage T1 and T2(P<0.05);positive rate of PPARγin patients with poor differentiation was 68.2% ,significantly higher than 42.9% in patients with high or middle differentiation group(P<0.05).Conclusion PPARγ plays an important regulating role in the onset and progress of bladder urothelial cancer ;PPARγexpression level was correlated with primary tumor size ,pathological types and differentiation degree ,lymph node me‐tastasis and clinical stage.This result suggested that PPARγ was closely correlated to metastasis of bladder urothelial cancer.