Objective To investigate the psychological status of organ donation coordinators. Methods The anxiety, depression, sleep quality and personality characteristics of 40 organ donation coordinators were assessed by self-rating anxiety scale (SAS), self-rating depressive scale (SDS), Pittsburgh sleep quality index (PSQI) and Eysenck personality questionnaire (EPQ), respectively. Results The SAS and SDS scores of the organ donation coordinators were significantly higher than those of the norm group (t=23.372, 9.743; both P<0.05). The component scores of sleep quality, sleep latency, sleep duration, habitual sleep efficiency, sleep disturbances, use of sleeping medication and daytime function, and the total score of PSQI of the organ donation coordinators were significantly higher than those in the norm group (t=8.054, 7.274, 6.634, 10.613, 8.376, 19.166, 8.496, 17.372; all P<0.05). In terms of EPQ score, the N dimension score of male organ donation coordinators were considerably higher than that of the norm group (P<0.05). No statistical significance was identified in the N dimension score between the female organ donation coordinators and the norm group (P>0.05). The P,E and L dimension scores of the male and female organ donation coordinators did not significantly differ from those of the norm group (all P>0.05). Conclusions Organ donation coordinators present with varying degree of anxiety, depression and poor sleep quality, which deserves sufficient attention. Positive measures should be taken to mitigate these symptoms.