1.Evaluation and influencing factor analysis of the detection effect of hepatitis B virus surface antigen and treponema pallidum using rapid screening immunochromatography technology
Haiying SUN ; Enyong FAN ; Yudong JIAO ; Shouguang XU ; Chuming SHEN
Chinese Journal of Blood Transfusion 2023;36(10):931-934
【Objective】 To evaluate the effectiveness of rapid initial screening using HBsAg and syphilis reagents of immunochromatography technology before blood donation, and explore the influencing factors. 【Methods】 The pre-donation screening of HBsAg and anti-TP and post-donation blood test results of blood donors in Yangzhou region from January 2020 to June 2023 were retrospectively analyzed. The HBsAg and anti-TP reactive samples by ELISA from January to June 2023 were, retested using colloidal gold immunochromatographic reagents, and the results were compared and analyzed. 【Results】 A total of 200 414 blood donors were screened, among which 781 were HBsAg and anti-TP positive, accounting for 0.39%. A total of 191 717 blood donors successfully donated blood, and 986 were HBsAg and anti-TP positive by ELISA, accounting for 0.51%. 62 HBsAg and 61 anti-TP reactive samples were retested using the initial screening reagent, with 24 HBsAg reactive samples and 26 anti-TP reactive samples, accounting for 38.71% and 42.62% respectively. 14 HBsAg and 6 anti-TP gray area samples were retested, but no reactivity was found.The reactivity rates of 9 samples with HBsAg detection S/CO values greater than 25.0 and 10 samples with anti-TP detection S/CO values greater than 15.0 were all 100%.There was a negative correlation between the reaction intensity (S/CO value) of reactive samples and interpretation time of initial screening reaction. 【Conclusion】 The rapid primary screening of hepatitis B and syphilis with immunochromatography technology among blood donors can effectively improve the quality of blood and the qualification rate of blood after collection. Through targeted training of primary screening staff, the quality of primary screening can be further improved, the rate of missed detection can be reduced, and costs can be saved, thus reducing the risk of transfusion transmitted infection and ensuring the health of blood donors.
2.Effect of combined catheter ablation of atrial fibrillation and left atrial appendage closure on left atrial structure compared with a single procedure.
Zhentao FEI ; Ming LIU ; Pengcheng YAO ; Mingzhe ZHAO ; Changqi GONG ; Mu CHEN ; Yudong FEI ; Binfeng MO ; Rui ZHANG ; Yichi YU ; Yuli YANG ; Qian WANG ; Wei LI ; Pengpai ZHANG ; Jian SUN ; Qunshan WANG ; Yigang LI
Chinese Medical Journal 2023;136(24):3010-3012
3.Expression of TRIM59 in Non-small Cell Lung Cancer and Its Correlation with Prognosis.
Haiying TIAN ; Dongyang ZHANG ; Rongjian XU ; Yi QIN ; Yaliang LAN ; Wenjie JIAO ; Yudong HAN
Chinese Journal of Lung Cancer 2020;23(1):21-28
BACKGROUND:
TRIM proteins are important members of E3 ubiquitin ligases, and many studies have confirmed that TRIM family members play an important role in the development of various tumors. We found that TRIM59 expression level in non-small cell lung cancer (NSCLC) was significantly increased through second-generation sequencing. The purpose of this study was to investigate the expression of TRIM59 in NSCLC and its relationship with the clinicopathological parameters as well as the prognosis of patients.
METHODS:
The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets were excavated to analyze the expression of TRIM59 mRNA in NSCLC and its relationship with the prognosis of patients; The expression of TRIM59 protein in 90 tumor tissues and adjacent tissues was detected by immunohistochemical staining, and the relationship between the expression of TRIM59 protein and clinicopathological parameters and prognosis was analyzed.
RESULTS:
Overexpression of TRIM59 mRNA in tumor tissues predicted poor prognosis. The expression level of TRIM59 protein was significantly higher in tumor tissues than in adjacent tissues, and TRIM59 protein expression was correlated with tumor size (P=0.007), tumor differentiation (P=0.009), tumor-node-metastasis (TNM) stage (P=0.003) and lymph node metastasis (P=0.003). Multivariate Cox regression analyses showed that along with TNM stage, overexpression of TRIM59 could be considered an independent prognostic factor for NSCLC patients.
CONCLUSIONS
The expression of TRIM59 is closely related to the prognosis of NSCLC patients, and it is an independent risk factor for NSCLC patients.