[Objective]To investigate the discrepancy on biological characteristics of different subculture osteoblasts in vitro.[Methods] Compare the morphology,AKP activities and mineralization nodules of 1,3,5,7 and 9 subculture osteoblasts,and make statistic of the result.[Results] There was no discrepancy within 1,3 and 5 subculture osteoblasts,but the 7,9 subculture was significantly worse than subculture 1 in the morphology,AKP activities and mineralization nodules.[Conclusion] The osteoblasts within 5 subculture were suitable for experiment in vitro.

Objective To disclose the relationship of the target muscle function and different time interval after nerve grafting reconstructed C5 root resection in young rats.Methods Model of C5 resection was set up in 48 18-day-old SD rats.The rats were randomly divided into C5 resection group,immediate repairing group,3 days delayed repairing group,and 6,9,12,15,18 days delayed repairing groups.Each group experienced nerve grafting bridged the C5 nerve root defection at its time interval.At 6 weeks postoperatively,electrophysiological and histochemical experiment were performed.Results There was no statistical difference among the data of CMAP amplitude and latency and weight of target muscles and number of distal myelinated fiber of immediate repairing group and those of 3,6 days delayed repair group at 6 weeks postoperatively,but compared with C5 resection group,the dates was statistically higher.There was no statistical difference between the data of C5 resection group and that of 15,18 days delayed repairing group.Conclusion Nerve reconstruction for C5 root injury in young rats within 0-6 days (equal to 0-4 months in human beings) has a satisfactory protective effect on target muscles.It suggests that the OBPP children who have the operation indication should undergo surgical management in 4 months after their birth.

Cancer-related fatigue (CRF) is associated with tumor and (or) anti-tumor treatment,which has a high incidence and seriously affects the life quality of patients.The etiology of CRF involves psychology,physiology and social factors.There are various hypothesis of pathogenesis and the inflammatory system may play an important role in the generation and development of CRF.A variety of inflammatory cytokines such as IL-1β,IL-6,TNF-α,CRP are significantly correlated with CRF.

Objective To disclose the relationship of neuronal protective effect and different time interval after nerve grafting reconstructed C5 root resection in young rats.Methods Model of C5 resection was set up in 18-day-old SD rats from Jauary 2009 to December 2009.Forty-eight rats with C5 resection were randomly divided into C5 resection group,immediate repairing group,three days delayed repairing group,and 6,9,12,15,18 days delayed repairing groups.Each group experienced nerve grafting bridged the C5 nerve root defection at its time interval.At 4 weeks postoperatively,the numbers of True Blue positively labeled neurons in all groups were counted respectively.Results There was no statistical difference among the number of proxinal neuron of immediate repairing group and those of 3,6 days delayed repair group (P ＞ 0.05),but compared with C5 resection group,the number of neurons was statistically higher (P ＜ 0.05).There was no statistical difference between the number of motoneurons of immediate repairing group and that of 9 days delayed repairing group(P ＞ 0.05),but there was statistical difference between sensory neurons of this two groups(P ＜ 0.05).The neuron number of inmediate repairing group was statistically higher than those of 12,15,18 days delayed repairing group(P ＜ 0.05).Conclusion Nerve reconstruction for C5 root injury in young rats within 0-9days (equal to 0-6 months in human beings) has a satisfactory protective effect on proximal neuron.It suggests that the OBPP children who have the operation indication should undergo surgical management in 6 months after their birth.

Objective Through detecting the levels of von willebrand factor (vWF) ,vWF-cleaving protease (vWF-cp)before and after percataneous coronary intervention (PCI) in patients with coronary atherosclerotic heart disease ,to evaluate the relationship between them .Methods According to the results of coronary angiography ,study objects were divided into control group (normal) and PCI group ( the diagnosis of coronary heart disease with PCI operation ) .Enzyme-linked immunosorbent assay (ELISA) was carrout to determin the levels of plasma vWF concentration and vWF-cp activity in plasma and statistical analysis .Results The pre-operative ,postoperative vWF levels in plasma in PCI group were significantly higher than that in the normal control group ;The plasma vWF levels after PCI were significantly higher than that in the group before PCI (P< 0 .05) ;vWF-cp level of the PCI group were lower than in the control group ,and postoperation is lower than preoperation ( P < 0 .05) .With the coronary artery lesion worsen ,the plasma level of vWF increased ,while the level of vWF-cp activity decreased .Conclusion vWF and vWF-cp levels with the severity of coronary atherosclerosis and thrombosis risk was increased in different decreased ,which may play an important role in the pathogenesis of acute coronary syndromes .Changes of vWF and vWF-cp level after PCI indicate that interventional therapy can increase the risk of thrombosis to some extent .

Objective To investigate the effects of nonadrenergic noncholinergic (NANC) neuro transmitter on the frequency and strength of the automatic contractility of detrusor strip. Methods Cystometry was performed to observe the occurance of detrusor instability (DI) after 6 weeks of Wistar rats LUT. Then the rats were sacrified and their detrusors were made into strips to observe the effect of NANC vasoactive intestinal peptide (VIP) and adenosine triphoate(ATP) on the frequency and the strength of automatic contractility. Results The occurrence rate of DI was 62% after 6 weeks of obstruction of LUT; VIP and ATP could significantly inhibit the automatic contraction ability of detrusor strip, decreasing the frequency and the strength of the automatic contraction of detrusor ( P 0.05). Conclusions VIP and ATP can significantly inhibit the frequency and strength of the automatic contractibility of normal and obstructive detrusor.

Cancer cells can secrete programmed death ligand 1 (PD-L1) to bind with inhibitory regulatory protein of programmed death 1 (PD-1) which lies on T-cell lymphocyte.Consequently,the activity of the T cells reduces,and the apoptosis cells increase.It's one of the pathways of newly discovered tumor immune escape.Primary hepatic cancer is one of the common malignant tumors of digestive system and closely related to viral hepatitis B in China.PD-1/PD-L1 pathway plays an important role in tumorigenesis and development of primary liver cancer.In this paper,the research updates of PD-1/PD-L1 in primary hepatic carcinoma were summarized.

ObjectiveTo evaluate the inhibiting effects of mitomycin C（MMC） on fibroblasts of human pterygium （HPF） in vitro．MethodsThe fibroblasts derived from the 5 th to 8 th generation of the cultured tissues in human pterygium．These fibroblasts were exposed to culture plates with MMC at 6 gradient concentrations，adjusting the concentration value of the culture medium to 105，104，103，102，10 and 1 μg/L．The cells had been treated with PBS in the control group．After 48 h of incubation，the absorbency had been determined individually by MTT［3-（4，5-dimethylthiazol-2-yl）-2，5-diphenyl-tetrazolium bromide］ method.The inhibitory rates were calculated accordingly．All the data were analyzed with student t test and linear regression．ResultsThe extensive breakage of fibroblasts was observed．There was a statistical difference between the tested group and control group in absorbency (P＜0.05)，especially at the concentrations of more than 100 μg/L (P＜0.01)．The degree of growth inhibition increased with exposure of cells to MMC over 48 h．It showed that there is a positive correlation between the doses of MMC and the antiproliferative effects on HPF．ConclusionMMC significantly inhibites the proliferation of HPF in a dose-dependent manner．

Objective To compare the differences of chemotaxis at the tissue specific level and topographic specificity level during peripheral nerve regeneration after the severance injury of the sciatic nerve in rats of different ages. Methods 40 specific pathogen free Sprague Dawley 18 day old rats (called as young group) were chosen to correspond as nearly as possible to humans of about 1 year of age. 40 adult rats (called as adult group) were chosen to correspond as adult humans. The tissue and topographic specificity models were set up respectively after the severance of the sciatic nerve at the right side. Electromyography and morphological evaluations were conducted for determination of the nerve regeneration on 3 and 6 weeks postoperatively. Results On 3 and 6 weeks after operation, the recovery rate of nerve conduction velocity, the exact rate of nerve regeneration, the ratio of nerve conduction velocity between tibial and peroneal nerve, the ratio of amplitude of CMAP between tibial and peroneal nerve, the ratio of the number of myelinated axon between the tibial and peroneal nerve at the distal part in the young group were lower than those in the adult group respectively. There were statistically significant differences between the 2 groups. The recovery rate of amplitude in compound muscle action potential (CMAP) was lower in the young group than in the adult group, but the difference was not significant enough. Conclusion Since the tissue and topographic specificities in the young rats are both inferior to those in the adult rats, co contraction between agonists and antagonists may result from cross innervation during the peripheral nerve regeneration.