1.Enzyme-instructed hybrid nanogel/nanofiber oligopeptide hydrogel for localized protein delivery.
Tianyue JIANG ; Yudi MA ; Xiao XU ; Qingchun JI ; Mingxing FENG ; Cheng CHENG ; Yang FENG ; Bingfang HE ; Ran MO
Acta Pharmaceutica Sinica B 2021;11(7):2070-2079
Enzyme-catalysis self-assembled oligopeptide hydrogel holds great interest in drug delivery, which has merits of biocompatibility, biodegradability and mild gelation conditions. However, its application for protein delivery is greatly limited by inevitable degradation of enzyme on the encapsulated proteins leading to loss of protein activity. Moreover, for the intracellularly acted proteins, cell membrane as a primary barrier hinders the transmembrane delivery of proteins. The internalized proteins also suffer from acidic and enzymatic degradation in endosomes and lysosomes. We herein develop a protease-manipulated hybrid nanogel/nanofiber hydrogel for localized delivery of intracellularly acted proteins. The embedded polymeric nanogels (CytoC/aNGs) preserve activity of cytochrome
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