Objective To investigate the neuroprotective effect of 2-methoxyestradiol (2ME2) on neurological functions and nerve cell apoptosis in rats with acute cerebral infarction.Methods Seventy-two adult male SD rats were randomly divided into sham-operated group,model group and treatment group (n=24).Middle cerebral artery infarction models in the later two groups were constructed by Longa method.Rats in treatment group were treated with 2ME2 (24 mg/kg) daily for 7 d,while rats in the sham-operated group and model group with physiological saline.Bederson standard scale was adopted to analyze the nerve function defect 7 d after operation.Caspase-3 protein expression in the rat brain tissues was analyzed by Western blotting and apoptosis index was analyzed by TUNEL.Results As compared with those in the model group (2.015±0.871),Bederson standard scale scores in the treatment group (1.235±0.849) were significantly lower (P＜0.05);as compared with the sham-operated group (0.286±0.160,7.8 ±6.9),the model group and treatment group had significantly increased Caspase-3 protein level and apoptosis index (P＜0.05),while Caspase-3 protein level and apoptosis index in treatment group (0.615±0.185,27.8±6.4) were significantlylower than those in the model group (1.109±0.224,34.9±5.9,P＜0.05).Conclusion 2ME2 can improve the neurological function,reduce the level of apoptosis in rat brain nerve cells and has a protective effect on nerve.

This paper collects information on the species resources of medicinal plants in Laos through various methods such as literature collation and analysis, sample line survey, visits and surveys, and initially obtains the medicinal plant resources in Laos, the use of folk herbs, and traditional medicine knowledge archives. It is found that Laos is rich with wild medicinal plant resources. There are 2 165 species (including varieties) belonging to 1 014 genera of 214 families, including 57 species belonging to 40 genera of 29 families of ferns, 32 species belonging to 17 genera of 8 families of gymnosperms, and 2 076 species belonging to 957 genera of 177 families of angiosperms. And, there are 108 kinds of medicinal materials currently circulatingin the market. Herbal resources is widely used in Laos, and many fresh herbs in the market have the special effects of relieving cough and promoting salivation, eliminating dampness, dispelling summer heat, nourishing yin and cooling blood.The medicinal materials for alleviating rheumatism and strengthening waist and knees in the market are with good prospects for development and utilization, so the development and management should be strengthened. China and Laos can conduct in-depth research and strengthen experience exchanges on traditional medicine experience.