Objective]Analysis about how Dai Sigong expounded and treated diarrhea. [Methods]Look up relevant literature,list Dai Sigong ’s expositions about diarrhea’ pathogenesis and treatments, and summarize how Dai Sigong treated diarrhea. [Results] Dai Sigong generalized some methods about diarrhea treatment, such“diaphoresis”“purgative method”“emesis method”“tonifying method”“harmonyzing method”“invigorating spleen for diuresis”“invigorating qi for ascending”“eliminating”dampness”“clearing interior heat”and so on.[Conclusion]Depanding on predecessors’ discourse analysis, Dai Sigong inherited Zhu Danxi’s academic thought, summariied diarrhea’ pathogenesis and treatments, made a breakthrough to a thought that“It’s noneffective not using diuresis to eliminate dampness”and put forword that“there are many ways to cure diarrhea by eliminating dampness”. It provided more means to therapy diarrhea for descendants and had great meanings to consult.

Objective To establish the quality standard for Bazheng Pills. Methods Radix et Rhizoma Rhei, Radix Glycyrrhizae and Fructus Gardeniae in Bazheng Pills were identified by TLC. Geniposide was determined by HPLC. Results Radix et Rhizoma Rhei, Radix Glycyrrhizae and Fructus Gardeniae could be identified by TLC. Geniposide showed a good linear relationship at a range of 0.187 2～ 2.808 0 ng, r=0.999 9.The average recovery was 100.70 % , and RSD was 1.01 % . Conclusion The established methods are simple and with good reproducibility, and can be used for the quality control of Bazheng Pills.

Objective To explore the significance of long non-coding RNA (lncRNA) taurine up-regulated gene 1 (TUG1) in hepatocellular carcinoma (HCC),to predict the target gene of TUG1,and to provide a ref-erence for further study of TUG1 in HCC.Methods The differential expression of TUG1 in HCC was ana-lyzed by using the UALCAN database and the survival analysis of TUG1 was performed.The target gene of TUG1 was predicted by RegRNA 2.0 biology software,HMDD,targetscan and microT-CDS,and the regulato-ry network of lncRNA TUG1-microRNAs-mRNAs was constructed.The predicted target gene was analyzed by Gene Ontology (GO) and KEGG signal transduction pathway enrichment by using FunRich platform. Results TUG1 expression in HCC was significantly increased,and the expression level of TUG1 increased generally with the increase of tumor grade.The overall survival of patients with low expression of lncRNA TUG1 was significantly longer than that of lncRNA TUG1 high expression patients.There were four possible binding sites of HCC related microRNAs (hsa-mir-122-5p,hsa-mir-200a-3p,hsa-mir-34c-3p,hsa-mir-629-3p) on TUG1,which regulated 245 downstream target genes and formed the regulatory network of lncRNA TUG1-microRNAs-mRNAs.In the biological process,microRNA target genes were highly enriched in the processes such as the regulation of nucleobase,nucleoside,nucleotide and nucleic acid metabolism.In KEGG pathway analysis,microRNA target genes were highly enriched to the signal pathways mediated by Syndecan and TRAIL.Conclusion TUG1 expression level in HCC increased.Increased expression of TUG1 is associat-ed with poor prognosis in HCC.Bioinformatics methods can be used to explore the mechanism of tumorigene-sis from the molecular level,which can provide valuable information for subsequent experiments and clinical diagnosis and treatment.