Aim To investigate the NIH3T3/STAT3CA cell proliferation ability and the STAT3 transcriptional activity affected by PTPMeg2 . Methods MTT assay and xenograft nude mice model were used to investigate the NIH3 T3/STAT3 CA cell proliferation inhibited by PTPMeg2 in vitro and in vivo. Co-immunoprecipitation assay was used to measure the interaction between PT-PMeg2 and STAT3CA. STAT3 transcriptional activity was measured by dual-luciferase assay. Results The NIH3 T3/STAT3 CA cell proliferation ability was signifi-cantly inhibited by PTPMeg2 in vitro and in vivo com-pared with the control group ( P <0.05 ) . The tran-scriptional activity was increased by PTPMeg2 , but not the PTPMeg2 mutant (PTPMeg2C515S) and the ShPT-PMeg2 . Conclusion PTPMeg2 plays a role in inhibi-ting the proliferation ability of NIH3 T3/STAT3 CA cells through inhibiting the STAT3 transcriptional activity.

Objective To investigate the action and the mechanism of Jinyin granule(JYG) on cholecystitis in guinea pigs.Methods Cholecystitis models of Guinea pigs were established and then randomly divided into six groups: normal group,model group,XiaoYanLiDan tablet(XYLDT) group,treatment groups including low,middle and high dosage groups of JYG.The Pigs were given above drugs respectively while in normal and model groups the same volume of normal saline was given.Body weight,gallbladder weight,bile amount,IL-1? in the serum were measured and gallbladder pathological tissue was observed by light microscopy.Results Comparing with the model group,the treatment groups had higher body weight,lower gallbladder weight,less bile amount,obviously slighter inflammation and less amount of IL-1? in the serum.Conclusion JYG possesses the therapeutic action to cholecystitis on guinea pigs and its mechanism is interrelated to the decreasing of IL-1? in the serum.

Objective To study the effect of attachment, coping style and social support on mental health in technical school students.Methods 372 technical school students were assessed by ECR(Experiences in Close Relationship's Inventory) ,Symptom Check Scale List-90 (SCL-90) and Coping Style Scale For Secondary School Students( CSS), Adolescent Self-Rating Life Events Checklist ( ASLEC), Perceived Social Support Scale (PSSS).Results ( 1 ) Life events had significant correlations with all indexes score of attachment anxiety ( r =0.293 ～ 0.356, P ＜0.01 ) ,the scale of coping style focus on emotion and the total score of SCL-90 ( r = 0.259 ～0.509, P＜0.01 ) ,and the total score of perceived social support had significant correlations with the total score of SCL-90 ( r =-0.238, P ＜ 0.01 ).( 2 ) Structural equation analysis ( model fit: χ2 = 540.593, RMSEA = 0.114,NFI = 0.864 ,CFI =0.884, P ＜ 0.01 ) showed that life events affect on mental health through the mediation effects of coping style focus on emotion, attachment anxiety and the perceived social support and coping style focus on emotion had most mediating effect on mental health ( mediation effect = 33.2% ).Conclusion Life events had both direct effect and indirect effect through coping style focus on emotion, attachment anxiety and the Perceived Social Support on mental health.

Objective: To study clinical characteristics and anatomical distributions of pulmonary embolism (PE) in suspicious PE patients and to explore if CT coronary angiography (CTCA) may simultaneously exclude PE. Methods: A total of 403 consecutive patients with suspicious PE admitted to cardiology department of our hospital from 2013-01 to 2014-10 were retrospectively studied. According to embolus distribution by CT pulmonary angiography (CTPA) and CTCA, the patients were divided into 2 groups: PE group,n=261 and Non-PE group,n=142. The clinical symptoms and imaging characteristics were analyzed and compared between 2 groups. Results: The overall prevalence of PE was 64.8% (261/403), suspicious PE patients were all with chest distress, palpitation, chest pain and syncope. Compared with Non-PE group, the patients in PE group were with more female gender and palpitation, while less chest pain,P<0.05; the symptoms of syncope and chest distress were similar between 2 group,P>0.05. In PE group, the sign of emboli were found in 245/261 patients (93.9%) at CTCA scanning area, the rest 16 patients (6.1%) had the small area of PE, and the emboli were only located at both upper pulmonary arteries. Conclusion: In patients with dififculty of breath, palpitation, chest pain and syncope, coronary artery disease (CAD) and PE should be simultaneously considered for accurate diagnosis. CTCA may meanwhile ifnd PE during CAD diagnosis.

Objective:To establish a simple and fast differential scanning calorimetry (DSC) method for identifying different gelatin Chinese herbal slices and formula granules.Methods:Adopting DSC method with 100 mesh sample size, making aluminum crucible as the reference, nitrogen was atmosphere, and the flow rate was 100 ml/min. The optimal temperature increasing range and temperature increasing rate are investigated. Based on the optimized DSC test conditions, 42 batches of Asini corii colla, Cervi cornus colla, Testudinis carapacis and formula particles were identified.Results:The optimal heating range of DSC test was 30-500 ℃, and the heating rate was 30 ℃/min. There were 4, 3, 2 aracteristic peaks of Asini corii colla, Cervi cornus colla, and Testudinis carapacis et plastri colla, and their peaks' shapes and positions were different. The antler gum formula particles and tortoise shell formula particles had two exothermic peaks, but the peak positions were different. There was one exothermic peak in the Asini corii colla. There is an exothermic peak in the traditional Chinese medicine decoction pieces and formula particles of antler gum formula particles at about 325 ℃. This exothermic peak can be used as the characteristic peak of antler gum formula particles.Conclusions:DSC has the advantages of simple operation, small amount, no reagents, fast measurement, good reproducibility, and easy analysis of the spectrum. And it can identify the value and size to distinguish between various gel pieces and formula granules.

Objective To investigate the contamination of aflatoxin B1 in traditional Chinese medicine decoction pieces.and to provide evidence for the development of sdandards and scientific management.Methods Immunoaffinity column and post-column photochemical derivatization were used to detect and quantify aflatoxin B1 in 35 traditional Chinese medicines.Results A total of 48.57％(17 out of 35 batches) traditional Chinese medicine were contained aflatoxin B1.The contents of aflatoxin B1 in all contaminated varieties were less than 1μg/kg,except for Sterculia lychnophorae Semen,Foeniculi Fructus,Corydalis Rhizoma,which exceeded the standard.Conclusions The tested traditional Chinese medicine are highly contaminated of aflatoxin,it is necessary to further study the increase of aflatoxin content under the examination of Chinese Pharmacopoeia Foeniculi Fructus and Corydalis Rhizoma to better control its quality.The degree of aflatoxin B1 pollution is reated to the site of drug use and the place of origin.

Objective To explore a quick and simple method to identify ginseng medicinal materials. Methods Twenty types of ginseng medicinal materials were collected. The effects of two important factors:temperature and heating rate on differential scanning calorimetry (DSC) were investigated. The DSC features were used to quickly identify Radix et Rhizoma Notoginseng, Radix et Rhizoma Ginseng, Radix Panacis Quinquefolii and Panna ginseng C.A.Mey. Results The optimal experimental conditions for rapid identification of Radix et Rhizoma Notoginseng, Radix et Rhizoma Ginseng, Radix Panacis Quinquefolii and Panna ginseng C.A.Mey by DSC were determined: temperature range 30-400 ℃, heating rate 30 ℃/min. Under these conditions, the characteristic peak temperature and thermal enthalpy value can be used to distinguish four kinds of ginseng. Conclusions The experiment shows that the DSC method has the advantages of simple operation, rapid measurement, good reproducibility, easy analysis of the spectrum and less sample consumption. It is a new method for quickly identifying Chinese medicinal materials of ginseng genus.

Objective:To study the method of intravascular angiography in vivo, analyze the clinical significance, and supply the basis of diagnosis and treatment of related orthopaedic diseases.Methods:The development was realized by improving the developer to increase the local concentration. Based on the study of Lijianmin-Chengkun Complexes and using the theory of magnetic microspheres, Fe 3O 4 magnetic microspheres with amino (negatively charged) shell are used to adsorb the aggregated ionic developer meglumine diatrizoate (positively charged diatrizoate). That is, by improving the method of developer, the magnetic microspheres can carry the developer to make new nanoparticles magnetic imaging composite particles. Under the action of external magnetic field, the magnetic imaging composite particles brought by blood circulation continue to stay and gather in the blood vessels in the magnetic field area, and the developer carried by the magnetic microspheres in the blood vessels in the magnetic field area is concentrated to reach the imaging concentration, so as to realize in vivo intravascular vascular imaging. By adjusting the ratio of the two reagents, the charge can be neutralized and condensed into small groups to improve the development efficiency. Thus, the electron microscope experiment, CT in vivo experiment, rabbit imaging experiment, experimental rabbit tissue picture confirmation, CT in vivo human body (the author is a volunteer) imaging experiment were carried out step by step. Results:Electron microscope experiment: meglumine diatrizoate, scanning electron microscope, the particle diameter is about 20 nm. Scanning electron microscope showed that the diameter of the magnetic microspheres was about 100 nm and the distribution was uniform. After the two reagents are mixed in a certain proportion, the neutralizing charge condenses into small groups, but it still has magnetohydrodynamic properties and strong paramagnetism. In vivo rabbit imaging experiment: the ideal intraosseous vascular imaging of the proximal tibia was captured. The tissue pictures of experimental rabbits confirmed that the distribution of Fe 3O 4 was obviously visible in the blood vessels in the proximal tibia on the side with magnetic field, but not on the side without magnetic field. In vivo human imaging experiment: the ideal intraosseous vascular imaging of the proximal fibula was captured. Conclusion:Through the preparation of new reagent of magnetic imaging composite particles (magnetic microspheres + meglumine diatrizoate), the concentration of in vivo bone developer can be achieved under the action of external magnetic field, and the in vivo external diameter ≥ 0.5mm can be achieved under CT thin-layer scanning.