ObjectiveTo investigate the prevalence and distributing feature of atrial fibrillation (AF) in Xinjiang Kazaks adult population.MethodsFour-stage selected random samples aged 30-89 years from Tacheng,Yili and Aletai were analyzed.An epidemical study of AF was performed including inquiring medical history,recording electrocardiogram and auscultation. ResultsA total of 22 514 adults were surveyed.The prevalence of AF in Xinjiang Kazaks adult population was 0.37％,which was increasing with aging.The prevalence was higher in men than in women (0.5％ vs 0.2％,P ＜0.01 ).In AF patients,23 was valvular AF.Ischemic stroke was the most frequent type and the stroke rate in the patients with AF was significantly higher than that without AF (6.0％ vs 1.2％,P ＜ 0.01 ).ConclusionsThe prevalence of AF in Xinjiang Kazaks adult population is lower than the reported national prevalence but patients with AF in this population would not like to take the necessary medicine. Therefore,the control of AF need to be reinforced.

BACKGROUND:Denis B thoracolumbar burst fractures are common spinal injury and may be involved in the upper end plate injury. Fracture reduction and pedicle screw fixation are used to repair above injury. This scheme can effectively achieve the aim of correcting deformity, but the trabecular bone after crushing cannot be fuly recovered. OBJECTIVE:To observe the repair effect of fracture reduction and pedicle screw fixation + artificial bone graft in vertebral body on Denis B thoracolumbar burst fracture, and compare with fracture reduction and pedicle screw fixation alone. METHODS:Clinical data of 70 cases of Denis B thoracolumbar burst fractures, who were treated in the Department of Orthopedics, Zhangjiagang Aoyang Hospital from January 2012 to December 2014, were retrospectively analyzed. According to repair scheme, they were equaly divided into two groups. Patients in the control group received fracture reduction and pedicle screw fixation. Patients in the observation group received fracture reduction and pedicle screw fixation + artificial bone graft in vertebral body. Oswsetry Disability Index, height of anterior border of injured vertebral body, lower back pain visual analogue scale and vertebral kyphosis Cobb’s angle were compared and observed between the two groups before repair, 1 week, 3 and 6 months after repair. RESULTS AND CONCLUSION:No significant difference in Visual Analogue Scale was detected at 1 week, 3 and 6 months after repair between the observation and control groups (P > 0.05). Oswsetry Disability Index was significantly lower in the observation group than in the control group (P < 0.05). No significant difference in the height of anterior border of injured vertebral body was detected between the observation and control groups (P > 0.05). Cobb’s angle was significantly lower in the observation group than in the control group (P < 0.05). These findings suggest that fracture reduction and pedicle screw fixation + artificial bone graft in vertebral body obtained good repair effects on Denis B thoracolumbar burst fracture, not only effectively corrected Cobb’s angle and the height of anterior border of injured vertebral body, but also helped to restore normal spinal loads by filing bone in the injured vertebral body.

Objective To discuss anatomical characteristics and clinical results of the lateral superior genicular artery perforator iliotibial band flap. Methods From September 1999 to July 2009, the origin,course, branches and distribution of the lateral superior genicular artery and blood supply of iliotibial band were observed on 40 sides adult lower limbs perfused with red latax. Five perforator iliotibial band and 4 perforator iliotibial band flaps of 9 cases were treated with anastomosis of lateral superior genicular artery perforating rami. Results The lateral superior genicular artery of 35 cases (80%) originates from the popliteal artery. Its originated external diameter was (1.8 ± 0.4) mm. It divides into ascending branch and descending branch, and they gives off septofascio-cutaneous perforator, or anatomoses the lateral uppermost genicular artery in the vastus lateralis muscle, and then formes the musculocutaneous perforator of iliotibial band. The originated external diameter of the larger perforator of iliotibial bands was ( 1.0 ± 0.2) mm and they provides for the skin and iliotibial band of lateral superior genicular area. Nine cases, including 5 cases of simple Achilles tendon defects, two cases of achilles tendon defects with skin defects, and 2 cases of lower extremity and foot soft tissue defects were treated with transplantation of the vascularized iliotibial band (flap) with perforator vascular anastomoses. All cases were followed up 6 - 92 months. The results showed significant improvement in "heel test" and Thompson sign in the rerupture of the Achilles tendon occurred. Four perforator iliotibial band flaps survived well. Conclusion The lateral superior genicular artery perforator iliotibial band flap is a practical, simple and new donor in the reconstruction of soft tissue and composite defects.

We discussed the influence of liquid nitrogen cryopreservation to survive capability of Babesia microti standard strain.The whole blood of mice infected with Babesia microti was put in liquid nitrogen to cryopreservation for 1 month,3 months,6 months,9 months,the whole blood was get out respectively and recovery at room temperature,and infected 3 mice respectively,100 μL/ mouse (the first generation after redissolution,the experiment group).In the same time,3 mice were also infected with Babesia microti as the animal conservation control group.When the infection rate was at a high level,the whole blood of the experiment group mice were injected into 3 normal BALB/c mice (the second generation after redissolution),to observe the changes of the Babesia microti form and proliferation situation,and also to observe the infection rate of the first and the second generation after redissolution in different conserving time.Compared with Babesia microti of animal subcultivation,the form of Babesia microti of liquid nitrogen cryopreservation changed a little.Small trophozoites,annular trophozoites,schizont and immature and mature merozoite and other form can also be seen.Compared with Babesia microti of animal subcultivation,the first time to see the worms and the time attaining to the high infection level were 1 to 2 days later,but for the second generation after redissolution,it is the same.There was no significant difference in different conserving time of 1,3,6,9 months.The influence of liquid nitrogen cryopreservation to survive capability and worm form of Babesia microti is a little,so liquid nitrogen cryopreservation can be a better way to conserving Babesia microti.

Objective: To study clinical characteristics and anatomical distributions of pulmonary embolism (PE) in suspicious PE patients and to explore if CT coronary angiography (CTCA) may simultaneously exclude PE. Methods: A total of 403 consecutive patients with suspicious PE admitted to cardiology department of our hospital from 2013-01 to 2014-10 were retrospectively studied. According to embolus distribution by CT pulmonary angiography (CTPA) and CTCA, the patients were divided into 2 groups: PE group,n=261 and Non-PE group,n=142. The clinical symptoms and imaging characteristics were analyzed and compared between 2 groups. Results: The overall prevalence of PE was 64.8% (261/403), suspicious PE patients were all with chest distress, palpitation, chest pain and syncope. Compared with Non-PE group, the patients in PE group were with more female gender and palpitation, while less chest pain,P<0.05; the symptoms of syncope and chest distress were similar between 2 group,P>0.05. In PE group, the sign of emboli were found in 245/261 patients (93.9%) at CTCA scanning area, the rest 16 patients (6.1%) had the small area of PE, and the emboli were only located at both upper pulmonary arteries. Conclusion: In patients with dififculty of breath, palpitation, chest pain and syncope, coronary artery disease (CAD) and PE should be simultaneously considered for accurate diagnosis. CTCA may meanwhile ifnd PE during CAD diagnosis.

Objective To analyze the fractional proteins and immunoreactivity of the soluble antigens from Babesia microti (B.microti),and find the candidate antigens for diagnosis with high sensitivity and specificity.Methods BALB/c mice were inoculated with B.microti-infected red blood cells by intraperitoneal injection.The B.microti were collected from the infected red blood cells when the infection rate reached its peak (infection rate ＞70％),then the soluble antigens were extracted by repeated freezing-thawing and ultrasonic method.The mice sera before and after the infection with B.microti for 7,14,21,28,35,42,49 and 56 days were also collected.The polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze protein components of the soluble antigens of B.microti and the Western blot was used to analyze the immunoreactivity of the soluble antigens with the pooled mice sera before and after the infection.The specific positive protein bands were identified by Liquid chromatography electrospray ionisation tandem mass spectrometry (LC-ESI-MS/MS),and the amino acid sequences of the proteins were analyzed by bioinformatics tools.Results The results from SDS-PAGE analysis indicated that the soluble antigens of B.microti showed distinct protein bands with the range between 12 and 185 × 103 (kDa,relative molecular mass,Mr),among which 9 main bands and 12 minor bands were obtained.In the Western blot analysis,the protein bands with Mr at 40 and 45 kDa could be recognized by pooled mice sera 7 days after infection;the protein bands with Mr at 40,45,54 and 95 kDa could be recognized by pooled mice sera 14 days after infection;the protein bands with Mr at 27,40,45,54,95 and 110 kDa could be recognized by pooled mice sera 21 days after infection.While,the protein bands with Mr at 27,40,45,54,95,1 10 kDa and other weak-reactive bands were recognized by pooled mice sera 28-56 days after infection,and the reaction became stronger with the infection continued.There were 336 proteins,including surface antigen,heat shock protein 70,seroreactive antigen,Eta subunit of chaperonin containing t-complex polypeptide 1 and unnamed protein products,were identified as the components of soluble antigens after mass spectrometry and sequence analysis.Conclusion The 40,45 and 54 kDa protein components from the soluble antigens of B.microti may be ideal candidate antigens for diagnosis,andtheir potential applications in diagnosing of human babesiosis deserve further study.

Objective To establish A1E3 and B1C4 monoclonal antibody-based ELISA for detecting circulating antigen of Schistosoma japonicum and explore its application value in the field. Methods The characteristics of A1E3 and B1C4 monoclonal antibodies were analyzed by SDS-PAGE and Western blotting. The SEA-based ELISA was used to evaluate the titers of A1E3 and B1C4. The orthogonal test was used to determine the best concentration of coating antibody B1C4 and optimal working concentra-tion of A1E3-HRP. Under the optimal conditions,the serum samples of 20 acute schistosomiasis cases,46 chronic schistosomiasis cases,and 20 control sera were tested to evaluate its detection sensitivity and specificity. Seventy-two antibody positive serum sam-ples from Jiangling County of Hubei Province were detected and compared to a commercially available ELISA kit,to evaluate the detection effects of this method. Results The results of SDS-PAGE demonstrated that the purified A1E3 and B1C4 contained a clear heavy chain with molecular weight of 88 000 and 52 000 respectively and had the same light chain with molecular weight of 20 000;while Western blotting demonstrated that A1E3 and B1C4 could be recognized by SEA and serum samples of acute schis-tosomiasis cases. The SEA-based ELISA demonstrated the titers of B1C4 and A1E3 were 1∶105 and 1∶30 000,respectively. The serum samples from all the acute cases and 86.9% of the chronic cases showed a positive reaction. All of the control sera from healthy persons gave a negative response. The positive rates of the double monoclonal antibody ELISA and commercial ELISA for detecting the circulating antigen were 45.8%and 43.1%respectively,and there was no significant difference between the results of the two methods. Conclusion A1E3 and B1C4 monoclonal antibody-based ELISA is established successfully. It exhibits a high sensitivity and specificity in detecting circulating antigen of Schistosoma japonicum.

Objective To study the application of autoregressive integrated moving average(ARIMA)model to predict the monthly reported malaria cases in China,so as to provide a reference for prevention and control of malaria. Methods SPSS 24.0 software was used to construct the ARIMA models based on the monthly reported malaria cases of the time series of 2006-2015 and 2011-2015,respectively. The data of malaria cases from January to December,2016 were used as validation data to compare the accuracy of the two ARIMA models. Results The models of the monthly reported cases of malaria in China were ARIMA(2,1,1)(1,1,0)12 and ARIMA(1,0,0)(1,1,0)12 respectively. The comparison between the predictions of the two models and actual situation of malaria cases showed that the ARIMA model based on the data of 2011-2015 had a higher ac-curacy of forecasting than the model based on the data of 2006-2015 had. Conclusion The establishment and prediction of ARIMA model is a dynamic process,which needs to be adjusted unceasingly according to the accumulated data,and in addi-tion,the major changes of epidemic characteristics of infectious diseases must be considered.

Objective:To compare the clinical effects of mortice and tenon joint and end-to-end bone contact of zygomatic arch in reduction malarplasty, and to explore the clinical application value of mortice and tenon joint.Methods:The clinical data of patients who underwent reduction malarplasty in Sichuan Friendship Hospital and West China Hospital of Stomatology, Sichuan University from January 2013 to June 2020 were analyzed retrospectively. All patients were treated with classical or modified L-shaped zygomatic arch osteotomy. According to the bone connection mode of the zygomatic arch, the patients were divided into Group Ⅰ and Group Ⅱ. The patients in Group Ⅰ were treated with mortice and tenon joint (from June 2017 to June 2020), and the ones in Group Ⅱ were treated with end-to-end contact (from January 2013 to May 2017). The movement between zygomatic body and zygomatic arch were measured 6-24 months after surgeries. The postoperative satisfaction rate was investigated, and the incidence of complications within 2 years after operation was recorded. The data were statistically analyzed by SPSS 20.0 software. The measurement data were expressed by Mean±SD, and the counting data were expressed by cases (%). T-test was used to compare the age difference, zygomatic body and zygomatic arch movement distance between the two groups. Chi-square test was used to compare the sex composition ratio, postoperative satisfaction rate (sum of very satisfied and satisfied cases/sum of cases in respective group) and incidence of complications between the two groups. P<0.05 was considered statistically significant. Results:A total of 380 patients presenting with zygomatic protrusion were included in this retrospective study. One hundred and ninety patients were included in Group Ⅰ[22 men and 168 women; aged(26.8 ±8.6) years)] and 190 patients in Group Ⅱ [16 men and 174 women; aged (25.2 ±8.8) years)]. There was no significant difference in sex constituent ratio and age between the two groups ( P>0.05). After the operation, the width of the face was narrowed, and the contour of the face was significantly improved. The medial movement distance of zygomatic body in Group Ⅰ and Group Ⅱ was (2.65±0.76) mm versus (2.51±0.78) mm, and the movement distance of zygomatic arch in Group Ⅰ and Group Ⅱ was (4.58 ±0.44) mm versus (2.60±0.28) mm. There was no significant difference in movement distance of zygomatic body between Group Ⅰ and Group Ⅱ ( t=1.77, P=0.077), but there was significant difference in the zygomatic arch ( t=52.33, P<0.001). The satisfaction rate of patients in Group Ⅰ was 75.8% (144/190), which was higher than that in Group Ⅱ (55.3%, 105/190) ( χ2=17.72, P<0.001). There was no significant difference in the incidence of postoperative infection, temporary paresthesia, and hematoma between the two groups ( P>0.05). The incidence of facial asymmetry, sagging cheek, bone suture and nonunion in Group Ⅱ was significantly higher than that in Group Ⅰ ( P<0.05). Conclusion:Compared with end-to-end bone contact in reduction malarplasty, the mortice and tenon joint of the zygomatic arch achieved better facial narrowing effect and long-term stability, higher patient satisfaction rate and less postoperative complications.

Objective:To compare the clinical effects of mortice and tenon joint and end-to-end bone contact of zygomatic arch in reduction malarplasty, and to explore the clinical application value of mortice and tenon joint.Methods:The clinical data of patients who underwent reduction malarplasty in Sichuan Friendship Hospital and West China Hospital of Stomatology, Sichuan University from January 2013 to June 2020 were analyzed retrospectively. All patients were treated with classical or modified L-shaped zygomatic arch osteotomy. According to the bone connection mode of the zygomatic arch, the patients were divided into Group Ⅰ and Group Ⅱ. The patients in Group Ⅰ were treated with mortice and tenon joint (from June 2017 to June 2020), and the ones in Group Ⅱ were treated with end-to-end contact (from January 2013 to May 2017). The movement between zygomatic body and zygomatic arch were measured 6-24 months after surgeries. The postoperative satisfaction rate was investigated, and the incidence of complications within 2 years after operation was recorded. The data were statistically analyzed by SPSS 20.0 software. The measurement data were expressed by Mean±SD, and the counting data were expressed by cases (%). T-test was used to compare the age difference, zygomatic body and zygomatic arch movement distance between the two groups. Chi-square test was used to compare the sex composition ratio, postoperative satisfaction rate (sum of very satisfied and satisfied cases/sum of cases in respective group) and incidence of complications between the two groups. P<0.05 was considered statistically significant. Results:A total of 380 patients presenting with zygomatic protrusion were included in this retrospective study. One hundred and ninety patients were included in Group Ⅰ[22 men and 168 women; aged(26.8 ±8.6) years)] and 190 patients in Group Ⅱ [16 men and 174 women; aged (25.2 ±8.8) years)]. There was no significant difference in sex constituent ratio and age between the two groups ( P>0.05). After the operation, the width of the face was narrowed, and the contour of the face was significantly improved. The medial movement distance of zygomatic body in Group Ⅰ and Group Ⅱ was (2.65±0.76) mm versus (2.51±0.78) mm, and the movement distance of zygomatic arch in Group Ⅰ and Group Ⅱ was (4.58 ±0.44) mm versus (2.60±0.28) mm. There was no significant difference in movement distance of zygomatic body between Group Ⅰ and Group Ⅱ ( t=1.77, P=0.077), but there was significant difference in the zygomatic arch ( t=52.33, P<0.001). The satisfaction rate of patients in Group Ⅰ was 75.8% (144/190), which was higher than that in Group Ⅱ (55.3%, 105/190) ( χ2=17.72, P<0.001). There was no significant difference in the incidence of postoperative infection, temporary paresthesia, and hematoma between the two groups ( P>0.05). The incidence of facial asymmetry, sagging cheek, bone suture and nonunion in Group Ⅱ was significantly higher than that in Group Ⅰ ( P<0.05). Conclusion:Compared with end-to-end bone contact in reduction malarplasty, the mortice and tenon joint of the zygomatic arch achieved better facial narrowing effect and long-term stability, higher patient satisfaction rate and less postoperative complications.