1.Effects of hydrogen-rich saline on postoperative cognitive dysfunction in aged mice
Suwei TAO ; Yuchang XIN ; Xun ZHOU
Chinese Journal of Anesthesiology 2012;(11):1344-1346
Objective To investigate the effects of hydrogen-rich saline on postoperative cognitive dysfunction (POCD) in aged mice and the possible mechanism.Methods Seventy-two male C57BL/6 mice,aged 18 months,weighing 45-55 g,were randomly divided into 4 groups (n =18 each):control group (group C),hydrogen-rich saline group (group H),POCD group and POCD + hydrogen-rich saline group (group PH).POCD was induced by open tibial fracture.Hydrogen-rich saline 10 ml/kg was injected intraperitoneally twice a day for 7 days before surgery.Six mice were chosen at day 1,3 and 7 after surgery and cognitive function was assessed by Morris water maze test.The mice were then sacrificed and hippocampus was isolated for determination of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) contents (by ELISA) and nuclear factor-κB (NF-κB) activity.Results Compared with group C,the escape latency was significantly prolonged,the time of staying at the original platform quadrant was shortened,the frequency of crossing the original platform was decreased at 3 and 7 days after surgery,and the contents of NF-α and IL-1β and NF-κB activity were increased during 1,3 and 7 days after surgery in group POCD (P < 0.05),and no significant change was found in the parameters mentioned above in group H (P > 0.05).Compared with group POCD,the escape latency was significantly shortened,the time of staying at the original platform quadrant was prolonged,the frequency of crossing the original platform was increased at 3 and 7 days after surgery,and the contents of NF-α and IL-1β and NF-κB activity were decreased at 1,3 and 7 days after surgery.Conclusion Hydrogen-rich saline can alleviate POCD via inhibiting NK-κB activity in hippocampus and reducing inflammatory response.
2.Explore the judgmental of the indications of total knee arthroplasty using deep convolutional neural network
Ruijun CONG ; Longpo ZHENG ; Liyun ZHANG ; Kun TAO ; Wei LIU ; Xiangrong MO ; Youheng HAO ; Miao WANG ; Lieming LOU ; Xinyu CAI ; Yuchang ZHU
Chinese Journal of Orthopaedics 2018;38(7):418-424
Objective To explore the feasibility of the deep convolutional neural network (DCNN) judging the indications and prognosis of the total knee arthroplasty based on the trained DCNN computer learning system.Methods C1FAR-10 DCNN model based on TensorFlow (an open source system,Google,USA) optimized by Alex Krizhevsky were constructed.There were 400 cases with knee osteoarthritis from different databases used for analysis.Three hundred patients underwent total knee arthroplasty,while 100 did not.X-ray of 200 preoperative cases from the 400 cases and their enlarged image (50 times) were applied for training DCNN,while the enlarged images from other 200 cases were used to test the DCNN.The correlation and the regression between judgment of the DCNN and clinical truth were analyzed.The clinical truths were rechecked three times and were confirmed by treatment results.Pearson correlation and linear regression analysis were used.The relation test of the software was only used as a reference.Results There was no significant difference between the baseline of cases for learning and test.After learning 200 cases,the DCNN judged the 10 000 cases enlarged from remaining 200 cases.The correlation between the DCNN judgment and the clinical truth was not significant (r=0.000,F=0.001,P=0.970).False positive was observed in 1 681 cases,false negative in 3 296.After enlarged to 10 000 images,the correlation between the two judgments was significant (F=11 228.735,P=0.000,r=0.727 and R2=0.529).The software detection precision was 0.860.Conclusion DCNN can be applied in judging the indications of the total knee arthroplasty.Large sample size can improve the accuracy of the judgment significantly.
3. Mechanism of cleft palate in C57BL/6N mice induced by retinoic acid
Xiaozhuan LIU ; Yuchang TAO ; Xiuli ZHANG ; Zengli YU
Chinese Journal of Stomatology 2017;52(11):690-694
Objective:
To investigate the mechanism of cleft palate in mice induced by excessive all-trans retinoic acid (atRA).
Methods:
The pregnant mice were randomly divided into atRA-treated group (
4.Chinese Herbal Medicines Intervene in Signaling Pathways Associated with Diabetic Neuropathic Pain: A Review
Zhijun HE ; Xiaotao WEI ; Yuchang ZHANG ; Tao LIU ; Weiwei WANG
Chinese Journal of Experimental Traditional Medical Formulae 2022;28(17):275-282
Diabetic neuropathic pain (DNP) is one of the common complications of diabetic peripheral neuropathy, which is difficult to be cured. The clinical manifestations of DNP include reduced pain threshold, neuropathic pain in extremities, and abnormal pain. Studies have demonstrated that nuclear factor-kappa B (NF-κB), mitogen-activated protein kinase (MAPK), Toll-like receptor 4 (TLR4), nuclear factor erythroid 2-related factor 2 (Nrf2), and phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathways are involved in the treatment of DNP by inhibiting the activation and release of inflammatory cytokines [e.g., tumor necrosis factor-alpha (TNF-α) and interleukin (IL)], regulating inflammation-mediating factors [e.g., reactive oxygen (ROS), inducible nitric oxide synthase (iNOS), cyclooxygenase (COX-2), and NF-κB inhibitor (IκB)], suppressing neurogenic inflammation, destroying the persistent damage of peripheral nerves, and repairing damaged peripheral nerves. The literature of modern medical research has demonstrated that the treatment of DNP with Chinese herbal medicines is associated with the signaling pathways above. They can treat DNP by regulating the expression of key proteins in the signaling pathways to control neurogenic inflammation and repair diabetic peripheral neuropathic damage. By summarizing the available studies, this review aims to provide a reference for the in-depth research on Chinese herbal medicines in the treatment of DNP and give new insights into the protection of diabetic peripheral nerves and the treatment of DNP with Chinese herbal medicines.