1.Nursing of patients with gynecologic tumor and diabetes mellitus during chemotherapy
Yubin JIN ; Hongze LIU ; Yan LIU
Chinese Journal of Practical Nursing 2008;24(18):51-52
Objective To discuss the effect nursing of patients with gynecologic tumor and diabetes mellitus during chemotherapy in order to find effective nursing measure. Methods A retrospective study was carried out in 100 patients with gynecologic tumor and diabetes mellitus during chemotherapy. The treatment process was summarized. Results No incidence of infection and complications of diabetes mellitus were seen in these patients. The adverse effect of chemotherapy was low. Conclusions Strengthening of psychological and diet nursing, prevention of infection and health education proved to be effective measures to improve the life quality and prevent anxiety of patients with gynecologic tumor and diabetes mellitus during chemotherapy.
2.Effect of basic fibroblast growth factor on proliferation and differentiation of monkey bone marrow mesenchymal stem cells into neuronal precursor cells :Does the concentration affect cryptotanshinone induction?
Xiaogang LIU ; Yubin DENG ; Hui CAI
Chinese Journal of Tissue Engineering Research 2010;14(10):1813-1816
BACKGROUND:Basic fibroblast growth factor(bFGF)belongs to active peptide,which is an effective mitogenic factor.OBJECTIVE:To investigate the effect of bFGF on proliferation and differentiation of monkey bone marrow mesenchymal stem cells(BMMSCs)into neuronal precursor cells.METHODS:Monkey BMMSCs were in vitro cultured by density gradient centrifugation,and then divided into 4 groups after passaged,namely,control,bFGF with low,medium and high concentration groups.In the bFGF groups,0,3,6,10 μ/L bFGFwere applied.The proliferation of BMMSCs in each group were observed.The 5th BMMSCs were cultured with serum free L-DMEM culture medium containing 20 mg/L cryptotanshinone to differentiated into neuraMike cells.The expression of positive-nestin protein was detected by immunohistochemical method.RESULTS AND CONCLUSION:Compared with the control group,proliferation rate of BMMSCs in the bFGF groups were accelerated(P < 0.05),which showed a positive correlation to the concentration of bFGF.The positive-nestin protein could be found in the low and medium concentration groups at 0.5 hours after induction,and reached a peak at 1.5 hours,which increased obviously in the low concentration group than that of the high concentration group(P < 0.05).bFGF can promote BMMSCs proliferation in vitro,enhance inducing ratio of prophase neuron-like cells at lower concentration but inhibit differentiation at high level.
3.Influence of tissue culture on the survival of thyroid allografts in rats
Wenge LIU ; Zhiling PANG ; Yubin TAN
Chinese Journal of Organ Transplantation 1997;18(2):93-95
The thyroid allografts in rats were cultured for 48h in the conditions of hyperbaric oxygen (a mixture of 95% O2,5% CO2 pressurized at 2 atmospheres)and low pH(5.5 and 6.9).Uncultured and air cultured allografts were taken as control groups.The thyroid slices were transplanted under the kidney eapsulse of thyroidectomized recipients.By examining histological changes,detecting the serum T3 and T4 levels and five weeks body weight gain,the graft survival was cvaluated.Though the thyroids cultured in hyperbaric O2 were contaminated with the passenger leukocytes,the grafts had prolonged survival,suggesting this method might effectively diminish MHC immunogenicity to thyroid grafts.
4.Observation of dendritic cell and HLA-DR antigen expression in autoimmune thyroid diseases and subacute thyroiditis
Changxin JIANG ; Yubin TAN ; Yan LIU
Chinese Journal of Endocrinology and Metabolism 2000;0(06):-
Objective To investigate the effects of dendritic cell (DC) and HLA-DR antigen expression in the generation and development of Graves′ disease (GD), Hashimoto′s thyroiditis (HT) and subacute thyroiditis (SAT). Methods A morphologic study was performed on the thyroid tissue of 53 GD with pronounced lymphocytic infiltration, 52 HT and 31 SAT to investigate the pathologic changes of DC and HLA-DR antigen positive cell in the parenchymal cells and intestitial tissue by histochemical, immunohistochemical and electron microscopic technique and statistic analysis. Results The observation on DC and HLA-DR positive infiltrating cell in GD, HT and SAT showed a similar increase with the degree of cellular infiltation. The highest peaks of DC and HLA-DR positive thyroid follicular epithelium were in HT O-type and granulomatous area of SAT. The highest peaks of HLA-DR positive immunoactive cells was in HT P-type and fibrous area of SAT. Conclusion DC and HLA-DR positive cells may play an important role in the antigen-presenting step as well as in their direct cytotoxicity. These processes are related to the pathogenesis of AITD (GD and HT) and SAT. The thyroid follicules may be destroyed in the autoimmune reaction, and initially the patient presents hyperthyroidism and finally hypothyroidism follows because of the fibnosis of the follicules.
5.Approach on clinical application of visual artificial simulation splenectomy
Yubin LIU ; Chihua FANG ; Ye LIN
Medical Journal of Chinese People's Liberation Army 1981;0(06):-
Objective To study the three-dimensional (3D) reconstruction of pancreas based on the data of 64-row helical CT scanning and the effect of the dynamic simulation surgery of spleen.Methods The original scan data of spleen 64-slice helical CT were collected and the image program segmentation and automatic extraction were done with images of CT series by adaptive region growing algorithm.Then the image processing software was used to proceed with the 3D reorganization with the images which are segmented.Import reconstructed objectives to FreeForm Modeling System in the form of STL format were embellished and smoothed.GHOST SDK software of FreeForm Modeling System was utilized to develop various instruments needed by simulated surgeries.And the visualization of simulated surgery by PHANTOM software was studied before the splenectomy.Results By the adaptive region growing algorithm, the spleen image procedure could be calculated soon and efficiently, and the satisfactory spleen segmentation data were obtained.The clear picture structure of 3D- reorganization was a exact reproduction of spleen and the structures of vital organs and passages around;The simulated splenectomy was high simulated, vivid, exact and the operator could feel like a real surgery.Conclusions The research of 3D reorganization and visual artificial simulation surgery to spleen with the medical image process software and the FreeForm Modeling System can make surgeons know the 3D anatomical structure around spleen clearly before the surgery.And it is of great help to reduce the risk of surgery, decrease operative complications, and improve the effect of surgery.
6.Pathogenesis, diagnosis and treatment of postsurgical gastroparesis syndrome
Yubin XU ; Qian LIU ; Peijian ZHANG
International Journal of Surgery 2015;42(7):497-500
Postsurgical gastroparesis syndrome (PGS) is a functional disease,characterized by nauseating,vomiting,and gastric atony without of mechanical gastric outlet obstruction,and is ofen caused by operation at the abdomen,especially gastric and pancreatic operation.In recent years,the incidence rate of PGS presents ascendant tendency,and more and more doctor attach importance to PGS.A better knowledge of the etiology,pathogenesis,clinical manifestation,diagnosis and treatment of PGS can help decrease the incidence rate of PGS and improve the cure rate of PGS.Thus,the authors write this review about these aspects of PGS.
7.The influence of cognitive therapy on life quality of rural breast cancer patients during chemotherapy
Yubin JIN ; Hongze LIU ; Xudong CHEN ; Baofeng LIU ; Yingfeng LI
Chinese Journal of Practical Nursing 2010;26(16):7-9
Objective This study is to discuss the effects of cognitive therapy on life quality of rural breast cancer patients during chemotherapy in order to offer evidence for choosing the appropriate therapeutic measures.Methods 120 rural breast cancer patients were equally divided into the intervention group and the control group at random.The usual care plus cognitive treatment was given to the patients in the intervention group for 6 months,while only the usual care after chemotherapy was given to the control group.The quality of life assessments were carried out to both groups on the 5th day during hospitalization,and the first,third and 6th month during recheck period of time,respectively.The treatment effect was observed.Results There were statistical significant difference of the total scire of life quality assessment on the 5th day,the first,third and 6th month,respectively,between the intervention group and the control one after cognitive intervention.The downtrend of total score of quality of life in the intervention group after cognitive intervention was more significant than the control group,which indicated the quality of life of the intervention group was better than the control group. Conclusions Cognitive therapy is an efficient method to enhance the quality of life of rural breast cancer patients during chemotherapy.
8.Effects of Rosuvastatin and Losartan on expression of caveolin-1 in cultured human monocyte-macrophage cells induced by oxidized low density lipoprotein
Yubin HE ; Lina LIU ; Zheng WEI ; Pengjuan JI ; Xiaoyu SHEN
Journal of Chinese Physician 2014;16(8):1052-1055
Objective To investigate the effects,mechanisms,and the optimum doses of Rosuvastatin and Losartan on expression of caveolin-1 in cultured human monocyte-macrophage cells which were induced by oxidized low density lipoprotein(ox-LDL).Methods Human-monocyte cells were separated and changed into the human monocyte-macrophage cells.The model of amerosclerosis was set up.These cells were incubated in different doses of Rosuvastatin(0.1,1.0,5.0 μmol/L) and Losartan (10,50,100 μmol/L),and then cultured in combination of two drags (5.0 μmol/L + 100 μmol/L).Expression of caveolin-1 mRNA was determined with real-time fluorescent quantitative polymerase chain reaction (RT-PCR).Results In ox-LDL group,caveolin-1 mRNA was decreased sharply relative to control group [(0.2533 ±0.00973) vs (0.9410 ±0.03677)] in a concentration-dependent manner (P <0.01).Compared to ox-LDL group,expressions of Caveolin-1 mRNA were increased gradually in different doses of Rosuvastatin alone and Losartan alone group [(0.5198 ± 0.04840),(0.6183 ± 0.06740),(0.7257 ± 0.03052) vs (0.2533 ± 0.00973) ; (0.3350 ± 0.04177),(0.4428 ± 0.03804),(0.6049 ± 0.02627) vs (0.2533 ± 0.00973)] in a concentration-dependent manner (P < 0.01) ; the summit expressions of caveolin-1 mRNA were emerged in using Rosuvastatin and Losartan together (F =59.119,P < 0.01).Conclusions Rosuvastatin and Losartan may be responsible for the expression of caveolin-1 in human monocyte-macrophage cells that were induced by ox-LDL.The expressions were up-regulated with dose dependent manner of these drugs,and got the crest stage when using optimum doses of Rosuvastatin and Losartan together.
9.Expressions of EphA2 and EphrinA1 in bladder carcinomas and their clinical significance
Jinqing WANG ; Yubin ZHAO ; Shaohua ZHANG ; Feng LIU
Chinese Journal of Postgraduates of Medicine 2010;33(35):25-27
Objective To investigate the expressions of EphA2 and EphrinA1 in bladder carcinomas and their clinical significance. Method The expressions of EphA2 and EphrinA1 were detected on bladder carcinomas (75 cases ) by immunohistochemistry S-P method. Results ( 1 ) The positive rates of expression of EphA2 and EphrinA1 were 61.3%(46/75) and 46.7%(35/75) respectively. (2) The expression of EphA2 was related to tumor size, lymphatic metastasis and TNM staging(P< 0.05),but not to gender,age and pathology type (P > 0.05 ). ( 3 )The expression of EphrinA1 was related to tumor size and lymphatic metastasis (P < 0.05 ), but not to gender, age, pathology type and TNM staging (P > 0.05 ). (4)There was significantly positive correlation between the expression of EphA2 and EphrinA1 (P < 0.05).Conclusions EphA2 and EphrinA1 are related to the occurrence and development of bladder carcinoma.The combined detection of EphA2 and EphrinA1 can help to predict the clinical and pathologic characteristics of bladder carcinoma. It is an important index to assist treatment, they are predictive factors for bladder carcinoma.
10.Air Circumstance in Laminar Flow Operating Room in Beijing:A Crosssection Investigation of 18 Hospitals
Rong LIU ; Yinghong WU ; Yubin XIN ; Suying LI ; Xiuling ZHONG
Chinese Journal of Nosocomiology 2006;0(07):-
OBJECTIVE To investigate the management and sanitation status of the air circumstance in laminar flow operating rooms of grade two and above hospitals in Beijing.METHODS Air surveillance methods,cleaning methods of intakes,cleaning status of pipelines and frequency of renewing filter systems were investigated through interviewing charge nurse of 18 hospitals by questionnaires.RESULTS 50.0% of pipelines had never been cleaned,14.3% of intakes had never been cleaned.Air surveillance method of all hospitals was using sedimentation,with a low sensitivity.The monitoring systems were poor in 50.0% of hospitals.CONCLUSIONS Now there is only requirements of construction standard of clean surgery region,lacking routine regulations and technologies of contamination controlling standard,nor available dynamic contamination controlling system.Potential hazard exists in routine regulations of laminar flow operating room and it demands for making contamination controlling standard urgently in hospitals.