BACKGROUND:Basic fibroblast growth factor(bFGF)belongs to active peptide,which is an effective mitogenic factor.OBJECTIVE:To investigate the effect of bFGF on proliferation and differentiation of monkey bone marrow mesenchymal stem cells(BMMSCs)into neuronal precursor cells.METHODS:Monkey BMMSCs were in vitro cultured by density gradient centrifugation,and then divided into 4 groups after passaged,namely,control,bFGF with low,medium and high concentration groups.In the bFGF groups,0,3,6,10 μ/L bFGFwere applied.The proliferation of BMMSCs in each group were observed.The 5th BMMSCs were cultured with serum free L-DMEM culture medium containing 20 mg/L cryptotanshinone to differentiated into neuraMike cells.The expression of positive-nestin protein was detected by immunohistochemical method.RESULTS AND CONCLUSION:Compared with the control group,proliferation rate of BMMSCs in the bFGF groups were accelerated(P ＜ 0.05),which showed a positive correlation to the concentration of bFGF.The positive-nestin protein could be found in the low and medium concentration groups at 0.5 hours after induction,and reached a peak at 1.5 hours,which increased obviously in the low concentration group than that of the high concentration group(P ＜ 0.05).bFGF can promote BMMSCs proliferation in vitro,enhance inducing ratio of prophase neuron-like cells at lower concentration but inhibit differentiation at high level.

Hypoxia-inducible factor-1 (HIF-1), as a nuclear transcription regulator of the hypoxic response, is up-regulated during hypoxia, and it regulates a series of downstream target gene expression, such as vascular endothelial growth factor, glucose transporter and erythropoietin through binding with hypoxia response element. It plays important roles in angiogenesis, anerobic metabolism, cell survival, proliferation, migration, and differentiation.This article reviews the structure, function and activity regulation of HIF-1 and its roles in acute ischemic brain injury.

0.05).Conclusion Retropubic DVC control before laparoscopic radical prostatectomy is beneficial for both the surgical procedure and postoperative recovery of physiological functions,owing to minimal blood loss,clear surgical field,and less injury to the urinary continence system.

Objective To report the clinical outcomes of arthroscopic internal fixation by using anchor for tibial eminence fracture.Methods From January 2004 to January 2005,12 cases of tibial eminence were treated by arthroscopy with anchor fixation.Rehabilitation therapy was carried out after the operation.Results The mean operation time was 41 minutes(range 37 to 47).No patient had postoperative complications.They were followed up for 15 to 27 months(mean,23 months).The postoperative Lysholm score ranged from 91 to 98 with a mean of 93.The patients could extend the knee in a normal range,without pain or locking.Conclusion Arthroscopic internal fixation using anchor is a safe,simple,and effective treatment for patients with tibial eminence fracture.

Objective To evaluate laparoscopic radical prostatectomy for prostate cancer. Methods 8 patients presented clinical stages pT 1b to pT 2 prostate cancer.Laparoscopic radical prostatecomy was carried out transperitoneally with combining posterior and anterior approachs to the prostate,transecting the bladder neck,lateral dissection of the prostate and urethrovesical anastomosis. Results The operation time was 5 to 11 h with an average of 7.3 h and the blood loss 200 to 1 100 ml,averaged 620 ml.All the patients recovered well and uneventful with no complications such as urethral stricture or incontinence. Conclusions Laparoscopic radical prostatectomy is a better approach and least invasive.The procedure provides clear anatomic vision that facilitates operative performance and quicker recovery.

Objectives Hand-assisted laparoscopic nephrectomy in living donors was studied in order to investigate the advantages and disadvantages of this technique as well as the feasibility in clinic use.Methods Hand-assisted laparoscopic live-donor nephrectomy and transplantations were done in 6 canine models,the operating time,warm ischemic time,function of the donor kidney and the recovery of donor were all recorded and analyzed.Results All dogs survival postoperation in our experiment;the mean operating time was 132 minutes,the mean warm ischemic time of kidney was 83 seconds,however,the mean time of micturation after graft reperfusion was 74 seconds.Conclusions Hand-assisted laparoscopic live-donor nephrectomy is feasible with short operating time and warm ischemic time; the grafting function was well and donors recovered quickly postoperation,the minimal trauma;this technique is worth to be widely used in living donor nephrectomy.

Objective To evaluate the therapeutic effects of functional reconstructive technique of laparoscopic radical prostatectomy (LRP) for organ confined prostate cancer. Methods From October 2000 to September 2004,54 patients with organ confined prostate cancer (TNM stage of T 1b-T 2) underwent LRP.After completion of the first group of 15 consecutive cases (group A) by Monstouris techniques, the functional reconstructive surgical technique, which is basically composed of anatomical radical prostatectomy,was introduced to LRP in the second group of 39 consecutive cases (group B).These techniques mainly consisted of preservation of urethral and bladder outlet sphincter muscles,reconstruction of bladder neck,and fine anastomosis between urethra and bladder neck with fixation of anterior wall of anastomotic stoma and retropubic vascular complex.The operative time, bleeding volume,complications,continence recovery time and PSA level were comparatively analyzed between the 2 groups. Results All the operations were successful in 54 patients.In group A and group B,the mean operative time was 390 min(range,270-660 min)vs 240 min(range,180-360 min);the mean bleeding volume was 430 ml(range,200-1100 ml) vs 160 ml(100-400 ml);the complication rate was 40% (6/15) vs 13% (5/39) and the continence recovery time was on average 6 months vs 3 months,respectively. There were statistically significant differences in these parameters between the 2 groups (P

Objective To describe the ureteroplasty of congenital obstructive megaureter by laparoscopy and to evaluate the efficacy and feasibility of laparoscopic intervention for congenital obstructive megaureter. Methods Six patients with congenital obstructive megaureter were prepared for the laparoscopic surgery.The surgical procedure was briefly described as follows.The dilated ureter was dissected and cut off near the ureter orifice to the bladder by laparoscopy.Next,the free ureter was pulled out through the skin trocar site and was tailored as open surgery.After that,the ureter was placed back to the abdominal cavity and reimplanted laparoscopically into the bladder. Results The operation duration was 2 to 4 h (mean 2.4 h) and blood loss was very little (20～35 ml).No complication developed.The double J stent was removed at 3 months after the operation.Follow-up for half to 2 years showed that all the ureter drainage in the 6 cases was well without any infection. Conclusions Our preliminary result shows that laparoscopic surgery is a safe and effective method for treatment of megaureter.

Objective To explore the current status of humanistic care among medical students in clinical practice in obstetrics and gynecology department and its related influencing factors and to propose the corresponding countermeasures.Methods Scale of humanistic care quality was used in the survey for clinical practice students in obstetrics and gynecology department.ResultsThe total score rate of human care quality of medical students before clinical teaching were (84.12 ± 9.24)with a scoring rate of 72％.Significant differences were observed in the medical students with different medical professional(P ＜0.05).The total score of humanistic care quality of medical students after clinical teaching were (96.41 ± 1 1.53 ),significant higher than that of before training ( P ＜ 0.05 ).ConclusionHumanistic care quality in medical students needs to be strengthen.Clinical training in obstetrics and gynecology department combined with humanities education has important practical significance to improve the quality of medical students.

BACKGROUND:Human has a high level heritability in physical performance. With the development of technology and test method in molecular biology, the researchers of sport science are concerned with the influence of gene variation on the elite athlete performance. They begin to know the important value of gene on predicting the physical performance.
OBJECTIVE:To review the research results in the field of gene polymorphisms and elite athlete performance and to expatiate the problems in these researches, thereby offering some proposals.
METHODS:A computer-based online research of PubMed and CNKI databases was performed to col ect articles published from 1998 to 2013 with the key words“elite athlete performance, gene polymorphisms, endurance, power, training response”in Chinese and English. There were 150 articles after the initial survey. A total of 80 articles were included according inclusion and exclusion criteria.
RESULTS AND CONCLUSION:The researches of this field are mainly focused on the three aspects:elite endurance performance, elite power performance, and training response, which are associated with gene polymorphisms. The main genes related to elite endurance performance are ACE, mtDNA, PPAR, ADR, GNB3, NRF2, etc. The main genes related to elite power performance are ACTN3, ACE, GDF-8, IL-6, HIF-1, etc. The main genes related to training response are HBB, TFAM, NRF2, AR, FECH, etc. Several gaps in the current researches have been identified including smal sample size of most athletic cohorts, lack of corroboration with replication cohorts of different ethnic backgrounds. The numerous research findings can be applied to the gene selection of athletes by creating some kinds of algorithms and models.