Objective To summarize our experience on the diagnosis and treatment of gastric smooth muscle tumor.MethodThe clinical data of 34 cases with gastric smooth muscle tumor were analyzed retrospectively.ResultLeiomyosarcomas were ≥5 cm in diameter in 68% of all cases, and ≥10 cm in 50%. Most leiomyosarcomas were located in the fundus and corpus of the stomach (94%). Some patients of gastric leiomyosarcoma have the triad of epigastric pain, mass, and bleeding. Gastroscopy examination often reveals a mass lying submucously. Total or partial gastrectomy was performed for all patients based on tumor size and locus. The 3, 5, 10-year′s survival rate were 87%, 52% and 26% respectively. Conclusion Most gastric leiomyosarcomas are characteristic of local invasiveness, postoperative survival is often favourable.

Objective To compare the solution rate of berberine in Gegen Qinlian decoction and Chinese goldthread decoction,to clarify the scientific nature of the traditional Chinese medicine compound.Methods High performance liquid chromatography(HPLC)was used to detect the content of berberine in Gegen Qinlian decoction and Chinese goldthread decoction.Chromatographic condition was C1 8 chromatographic column,mobile phase was acetonitrile:0.05mol/L of potassium dihydrogen phosphate(48:52)(0.4g sodium dodecyl sulfate was added to per 1 00mL solution,adjusted pH to 4.0 with glacial acetic acid),detection wavelength was 345nm,the sample size was 1 0μL,flow rate was 1 .0mL/min,column temperature was 30℃.Results The average decoct rate of berberine in Gegen Qinlian decoction was 0.73%,while that was 0.45% in Chinese goldthread decoction,there was significant difference between the two groups(P <0.01 ).Conclusion The efficacy of TCMcompound Gegen Qinlian decoction is greater than the efficacy of the unilateral simple accumulation,so traditional Chinese medicine compound has certain scientific nature.

OBJECTIVE: To evaluate the effect of microwave polymerization and water-bath heat polymerization on accuracy and flexural strength of denture base resin. METHODS: Ten maxillary complete dentures were prepared and divided into microwave polymerization and water-bath heat polymerization groups according to random number table, with 5 dentures in each group. In the microwave polymerization group, the dentures were heated for 9 minutes with 40% firepower, natural cooled, the pressure of flasking at 4.5 kg/mm~2. In the water-bath heat polymerization group, the temperature increased from room to 70℃, sustained for 1.5 hours, then rise to 100℃ for 0.5 hour. The dentures were maintained at 25℃ thermostatic water bath for a week. The denture base, as well as standard cast was cut down at posterior border of the second molar. Dimensional accuracy between the posterior border of upper complete denture and standardized cast at five different positions was measured by digital microscope. Specimens with 64 mm× 10 mm×2.5 mm were made for measuring flexural strength.RESULTS: ①The posterior border gaps between the upper complete denture and standardized cast was (141±78) urn and (147±74) μm cured by microwave polymerization and water-bath heat polymerization, which has no statistical difference. The flexural strength of denture base resin cured by the microwave polymerization and water-bath heat polymerization was (81.60±14.04) MPa and (84.24±17.65) MPa, and there was no statistical difference (P > 0.05). CONCLUSION: There is no significantly difference in the accuracy and flexural strength cured by microwave polymerization and by water-bath heat polymerization, but the microwave polymerization takes shorter time than that by water-bath heat polymerization.

ObjectiveTo determine the allele frequency and genotypic distribution of plateletactivating factor acetylhydrolase (PAF-AH) gene polymorphism in the patients with sepsis. Methods Ala379Val,Val279Phe site genotypes were determined in patients (n=66) and healthy controls(n=68) by means of restriction fragment length polymorphism analysis of polymerase chain reaction products,DNA sequencing was used to detect the PCR product containing allele gene polymorphism.SPSS13.0 statistical software was use to analyze.Results All the samples by PCR-RFLP analysis of the PAF-AH gene in the Ala379Val site have three kinds of genotypes: in 66 cases of sepsis group there were 1 homozygous Val/Val type,19 heterozygous Val/Ala type,46 homozygous Ala/Ala type.In 68 cases of control group there were 2 Val/Val type,22 Val/Ala type,44 Ala/Ala type.The Ala379Val allele frequency and genotypic distribution in the patients with sepsis was not significantly different from those in the healthy controls.No statistically significant difference was observed between the survival group and the death group ( P＞0.05 ).PAFAH gene of Va1279Phe polymorphism could have three kinds of genotypes.All 66 patients in the sepsis group were the homozygous Val/Val type.Control group 68 cases,only one case was homozygous Phe/Phe type,and the others were homozygous Val/Val type,not found heterozygous Val/Phe type.The Val279Phe genotypic distribution and allele gene frequency in the patients with sepsis was not significantly different from those in the healthy controls; no statistically significant difference was observed no statistically significant difference was observed between the survival group and the death group ( P＞0.05 ).ConclusionNo associations were found between PAF-AH gene Ala379Val and Val279Phe polymorphisms and sepsis susceptibility,prognosis and severity.

Objective To observe the effects of early postnatal exposure to low-dose m-tolyl methylcarbamate(MTMC) on neurobehavior and spatial learning and memory ability in adolescent and early adult mice,and to dertermine when to affect this ability.Methods ICR mice pups randomly divided into 5 groups received intraperitoneal injections(i.p.) of different doses of MTMC(5 mg?kg-1,0.5 mg?kg-1,0.05 mg?kg-1),DMSO solution and normal saline respectively every other day during postnatal days(PND) 3～13.The general physiological condition and neural development of mice during experiment were evaluated,and a battery of tasks,i.e.hole-board test,beam walking,open field,Morris water maze(MWM),was used to assess their neurobehavior and spatial learning and memory ability in different time.Results Scores were negatively correlated to the doses of MTMC by Spearman rank correlation analysis in beam walking on PND60(rs=-0.418,P

Objective To investigate the abilities of emotion recognition and social cognition in patients with idiopathic epilepsy and further explore the factors related to the impairments.Methods After 24-hour EEG monitoring,70 people with idiopathic epilepsy were administered with a neuropsychological battery for basic cognitive assessment and then were tested with the Eye Basic Emotion Discrimination Task (EBEDT) and the Eye Complex Emotion Discrimination Task (ECEDT).Results Compared with health controls,people with epilepsy got significantly lower score in recognizing happy (19.3±2.0 vs 19.9±0.2),angry (17.9±2.0 vs 18.9±1.0),sadness (18.1±2.3 vs 19.2±1.0),fear (16.4±1.9 vs 17.6±1.3),disgust (17.6±2.1 vs 18.6±1.2) and surprise (18.3±1.5 vs 19.1±1.1) (P＜0.05,respectively),as well as complex emotions ((23.3±4.2) vs (27.1±2.8),P＜0.05).There was no significant difference between the two groups in gender recognition (P＞0.05).Significant positive correlation was found between the total score of basic emotions and the mind reading (r=0.444,P=0.000) in the patient group.Correlation analysis revealed significant relation between the total correct number of emotion recognition and the level of anxiety,depression and the executive function in epilepsy group (P＜0.05,respectively).Significant associations were found between the ECEDT and the Digital Span test,the Stroop test,Beck Depression Index and Hamilton Anxiety Scale (P＜0.05,respectively).Conclusion People with epilepsy have general impairments of emotion recognition and theory of mind,the impairments may be associated with the state of mood disorder and the weakened executive function.

Objective To obtain the gene coding for the group 5 allergens from Dermatophagoides farinae ( Derf5 ) and predict its molecular characteristics. Methods The total RNA of D. farinae were extracted, and the gene Derf5 was amplified by RT-PCR with the primers designed according to previous sequence published in GenBank. The target gene was linked into pMD19-T Simple plasmid, sequenced and analyzed by bioinformatics software. Results The sequence homology reached to 97.8% between our sequenced result with one complete open reading fragment (ORF) and the reference. The gene encode an extracellular hydrophobic protein with 132 amino acid resides, one signal peptide from 1 to 19 position and one transmembrane domain from 1 to 19 position. The secondary structure was composed of extended strand (1. 52% ), random coil (7.58%) and alpha helix (90.91%). The encoded protein was deduced to have two Casein kinase Ⅱ phosphorylation sites. The similarity of the amino acid sequence of the group 5 allergens were 78% between D. farinae and D. pteronyssinus. Conclusion The gene Derf5 was cloned successfully, and its characteristics was primarily predicted.

OBJECTIVE To investigate the repressor SmeT and other sequences of efflux pump SmeDEF in the clinic isolates of Stenotrophomonas maltophilia to involve in antibiotic resistance. METHODS The mRNA level of smeD examined the resistance profile of the clinical strains to antimicrobials was by agar dilution and their reaction to pump inhibitor was investigated by RT-PCR,and the smeD-smeT fragment was amplified by PCR,and then sequenced.Adding poly(A) to 3′ end of RNA,applying 3′RACE,nested PCR,then sequence-analysis. RESULTS The amino acid sequences of the forepart of SmeT were conservative in all the 7 strains.In antibiotic-resistant strains with positive reaction to pump inhibitor,the intergenic sequences of smeD-smeT were different from those in sensitive ones.The variations in(-165 to-82)nt of smeT and several nucleic acids before the start codon of smeT,possibly involved in antimicrobial resistance.All isolates didn′t have Leu166Gln mutation within the SmeT protein of D457R,which was reported possibly associated with antibiotic resistance.The resistant strains with efflux positively inhibited had Asp218Glu substitution,different from the negatively-inhibited ones. CONCLUSIONS The expression level of SmeDEF is related with the antibiotic resistance of S.maltophilia.The possible resistance mechanism includes the variations in the intergenic smeD-smeT and/or smeT codon sequence.

Objective To evaluate the effect of recombinant human erythropoietin (rhEPO) combined with methylprednisolone sodium succinate (MPSS),compared to MPSS alone,in the treatment of neurological function of patients with acute spinal cord injury (SCI).Methods Twenty-one patients presenting in hospital within less than 8 hours after acute SCI were randomly divided into two groups,the control group (10 cases) and the intervention group (11 cases).The control group was treated by MPSS combined with placebo,while the intervention group received MPSS with rhEPO.Both groups received MPSS 30 mg/kg within the first hour,and if the patient was admitted within 4 hours,MPSS would be applied in the treatment with 5.4 mg/kg per hour in the subsequent 23 hours and till 47 hours if the patient was admitted within 4-8 hours after injury.The intervention group received 500 U/kg rhEPO on admission and another 500 U/kg in the next 24 hours,compared with the control group where placebo was used.The evaluation on neurologic function recovery was made on admission,24 h,72 h,one week,2 months and 6 months later,and statistical analysis was performed.Results The change in ASIA score: in the control group,the increase was seen from admission to 6 months after injury in terms of exercise,algesia and tactile sensation ((31.2±6.6) points vs.(57.8±9.8) points,(41.4±9.5) points vs.(64.3±10.6) points, (39.2±6.8) points vs,(61.5±11.3) points),the increase also took place in the intervention group ((29.5±7.2) points vs.(77.4±10.3) points,(39.7±7.2) points vs.(82.3±12.1) points,(37.4±6.2) points vs.(78.6±12.4) points).As time went on,the increase range in the intervention group became larger,compared with the control group.The difference between the two groups in ASLA score was statistically significant (P<0.05).There was no significant difference in red blood cell count and hemoglobin content of routine blood test between the two groups (P>0.05).Conclusion The application of MPSS combined with rhEPO within 8 hours after acute spinal injury may be more effective than MPSS with placebo in the neurologic dysfunction recovery.

Objective: To investigate HDAC5 expression in gastric cancer cell lines and its effects on the proliferation and apoptosis of the gastric cancer line SGC-7901. Methods: The expression patterns of HDAC5 and Twist1 in gastric cancer cell lines and normal gastric mucosal cells were detected by Western blot. The effects of HDAC5 and Twist1 on the proliferation and apoptosis of SGC-7901 cells were analyzed by MTT and flow cytometry, respectively. Results: The expression of HDAC5 and Twist1 in gastric cancer cell lines were significantly higher than that in normal gastric mucosal cells (P<0.05). HDAC5 knockdown significantly down-regulated Twist1 expression,inhibited cell proliferation, and induced apoptosis in SGC-7901 cells, whereas HDAC5 overexpression exhibited an opposite effect (P<0.05). Moreover, Twist1 knockdown significantly inhibited cell proliferation and induced apoptosis in SGC-7901 cells (P<0.05). Conclusion:HDAC5 may promote cell proliferation and inhibit apoptosis in gastric cancer cells by upregulating Twist1 expression, thus promoting the initiation and development of gastric cancer.