Uveitis is a kind of inflammatory disease affected in ocular posterior segment.Uveitis sustains a long duration and causes a significant damage of visual function.It often leads to serious complications,such as cystoid macular edema (CME),cataract,glaucoma,retinal detachment and choroidal neovascularization (CNV).In the pathological process of uveitis,inflammatory factors promote the release of vascular endothelial growth factor (VEGF) through a variety of pathways.It has been verified that VEGF level is elevated in vitreous in the patients with uveiticinduced CME.Intravitreal injection of anti-VEGF drugs,including ranibizumab and bevacizumab,can improve the visual acuity in most patients with noninfectious uveitic-induced CME and CNV.However,intravitreously repeated injections of anti-VEGF drugs may be required in some patients.Intraocular injection of anti-VEGF drugs is an optional and effective way for those with persist macular edema and not being suitable for the use of corticosteroid or immunosuppressive therapy.However,anti-VEGF drugs may affect the efficacy of corticosteroid.Therefore,more clinical and basic researches are still needed.The status and progresses in the use of anti-VEGF drugs for uveitisinduced CME and CNV were reviewed.

Aim To investigate the reversal effect of PARP-1 inhibitor PJ34 on cisplatin-resistance in hu-man lung adenocarcinoma A549/DDP cells and the mechanism. Methods A549/DDP cells were treated with PJ34 alone or combined with cisplatin. The effects of proliferation inhibition were assayed by MTT meth-
od. The apoptosis ratios of cells were analyzed by flow cytometry. The protein expression of PARP-1 and LRP, GST-π were measured by Western blot assay. Results PJ34 could inhibit the proliferation of A549/DDP cells alone. The non-toxic dose of PJ34 could signifi-cantly resensitize A 5 4 9/ DDP cells to cisplatin , induce
apoptotic,lower the expression of PARP-1 and resist-ance-associated protein LRP and GST-π. Conclusion PJ34 could inhibit the proliferation of A549/DDP cells and resensitize A549/DDP cells,partially reverse cisplatin-resistance in A549/DDP cells, with a proba-
ble mechanism relating to increased apoptotic rate,and lowered expression of PARP-1 and resistance-associat-ed protein LRP and GST-π.

Based on a self-made questionnaire,this research is carried on to analyze the consumption psychology of mobile phone text among 400 university students of different grades,and finally discovered:(1)Mobile phone text has become a universally popular way of social intercourse among modern university students,and been wider used inside intimate communities.Its convenience and low expense are primary reasons for its widespread usage among university students.(2) The consumption of mobile phone text among university students displays remarkable differences between different genders and grades.(3) Some students formed certain psychological and behavioral problems during the usage of mobile phone text including mobile phone dependence and auditory hallucinations,and so on.

BACKGROUND: After tissue-engineering products transplantation, angiogenesis played an important role in the function restoring of defective organs. The natural tissue-engineering materials had a wide application in tissue engineering due to its favorable biocompatibility and degradability, at the same time its pro-angiogenic function enhanced the achievement ratio of tissue-engineering products transplantation. Therefore, they attract much attention during recent years. OBJECTIVE: To summarize the research status of incubating induced angiogenesis of tissue-engineered natural scaffold, so as to give some theoretical basis for further study on clinical application of natural tissue engineering materials. METHODS: Relevant literatures in PubMed and Springerlink published between January1995 and June 2009 were searched by compute with the key words of "tissue-engineering products, natural materials" in English. While relevant Chinese articles in CKNI published between January1999 and June 2007 were also searched with the key words of "tissue-engineering natural materials, collagen, chitosan, fibrin" in Chinese. After primary selection, inclusive articles were those about study and experimental study of induced angiogenesis of tissue-engineered natural scaffold. Exclusive criteria: repetitive and obsolescent articles. A total 35 literatures were finally analyzed in accordance with the criteria. RESULTS AND CONCLUSION: The natural tissue engineering materials were synthesized by macromolecules out of normal tissue, whose multiple bioinformation provided signal for cells and benefited for cellular adhesion and maintenance. Collagen protein, fiber gel protein, and chitosan summarized in this study were beneficial for inducing angiogenesis but limited to mechanical characteristics. Therefore, to construct natural materials inducing angiogenesisis is prospect.

Objective To investigate the protective effects of different concentrations of propofol on thelungs apainst acute injury induced by lipopolysaccharide (us) in rats.Methods Twenty-four male Wistar ratsweighing 150-250g were randomly divided into 4 groups with 6 animals in each group : (1) control group receivedonly normal saline; (2) LPS group received LPS 5 mg?kg~(-1)i. v.; (3) propofol group 1 received a bolus 5 mg?kg~(-1) after LPS followed by propofol infusion at 5 mg?kg~(-1) ; (4) propofol group Ⅱ received a bolus of propofol 10mg.kg~(-1) after LPS followed by propofol infusion 10 mg?kg~(-1). Blood samples were obtained from femoralartery for determintiion of serum concentrations of TNF-?, IL-? and IL-10 at 1, 2, 3, 4 h after LPS injection. Theanimals were then killed by exsanguination. The lungs were removed. Left lung was lavaged and the broncho-alveolar lavage fluid (BALF) was collected for determination of its neutrophil count, and protein, TNF-?, IL-I?and IL-10 levels. Right lung was used for measurement of wet / dry lung weight ratio. Results In LPS group thewet/dry lung weight ratio, BALF neutrophil counts and protein contents and BALF and serum TNF-?, Ib-I? andIL-10 levels were significantly increased compared with control group (P

Objective To investigate the effects of thiopental on expression of nuclear factor kappa B (NF-?B) and content of TNF-? and IL-1? in the lungs induced by lipopolysaccharide (LPS) .Methods Twenty-four male Kunming mice weighing 15-25 g were randomly divided into 4 groups (n = 6 each) : I control group received intraperitoneal (i.p.) normal saline (NS) 1 ml?kg-1 ; Ⅱ LPS group received i.p. LPS 5 mg?kg-1 ; Ⅲ LPS + thiopental (TH) received intrapentoneal TH 60 mg?kg-1 20 min after i.p. LPS 5 mg?kg-1 and Ⅳ TH group received i.p. TH 60 mg?kg-1 alone. The animals were bled to death at 3 h after LPS administration. The lungs were immediately removed for determination of expression of NF-?B p65 (Western blot) and TNF-? and IL-1? content (ELISA) in the lung. Results The expression of NF-?B p65 was significantly increased and the level of TNF-? and IL-1? in the lungs were significantly increased after LPS stimulation as compared with control group ( P

Objective To explore clinical efficacy of local random flap in reconstruction of facial skin and softtissue defects caused by lesions resection and injury.Methods Forty-six cases with facial skin and soft-tissue defects were cured by constructing and transplanting different local random flap that is designed according to parts,shapes and sizes of defect between July of 2008 and June of 2013.Results All the cases obtained satisfactory effects for full survivorship of flap,one-stage healing of wound,tiny scar and pigmentation,and good appearance by following up from 3 months to 6 months after operation.Conclusions Using local random flap in repair of facial skin defect could get satisfactoried functional and aesthetic efficacy,and it is worth being applicatied in the clinic.

Objective To establish a new multiplex-PCR assay to improve the detection rate of mutations in the DMD gene in Chinese patients. Methods A retrospective review of DMD deletion spectrum of 355 DMD patients with deletions all over the gene was performed. All deletions were confirmed by " one-step approach" diagnostic procedure and MLPA analysis. The exons with high frequency of mutations were identified to constitute the amplification system and the PCR conditions were optimized. Results Two new multiplex-PCR assays were established. Assay one was used to detect 10 exons including exon 5, 8, 17, 44, 45, 47, 49, 50, 51 and 52 of DMD gene, in two PCR sets. The theoretical detection rate would be 92% (326/355). Assay two was used to detect 5 exons including exon 12, 19, 35, 43 and 54, which could be used to screen additional 5% (17/355) deletion cases. The method was validated in other 22 DMD patients. Multiplex-PCR results were completely identical to the MLPA results in all 22 DMD patients. Conclusions The two multiplex-PCR assays were established based on the analysis of 355 Chinese DMD patients with gene deletions. It is believed that the new approach would be more applicable for deletion detection on the Chinese DMD patients since the DMD cases involved were from the whole country.

Objective To investigate the efficacy and safety of oxycodone hydrochloride controlled-release tablets combined with gabapentin in the treatment of painful diabetic neuropathy.Methods Eighty patients with painful diabetic neuropathy were divided into group A and group B with 40 cases each by random digits table.Patients of group A received simple oxycodone hydrochloride controlled-release tablets,while patients of group B received oxycodone combined with gabapentin.The intensity of pain, oxycodone dosage, quality of life and adverse effect in two groups was observed and compared.Results The pain was obviously relieved in two groups, and the effective analgesic rate at 21-28 d after treatment in group B was superior to group A[95.0% (38/40) vs.62.5% (25/40)], there was significant difference between two groups (P＜0.05).Numerical rating scale NRS score at 28 d after treatment in group B and group A was 1.2 ± 0.3 and 2.8 ± 0.5 respectively,and there was significant difference between two groups (P＜ 0.01 );the oxycodone dosage at 28 d after treatment in group B was lower than that in group A[(32.2 ± 4.3 )mg vs.(40.3±5.5 ) mg], and there was significant difference between two groups (P ＜ 0.05 ); the quality of life in group B was superior to group A, and there was significant difference between two groups (P ＜ 0.05 or ＜ 0.01 );the incidence of dizzy at 7, 14 d after treatment in group B was higher than that in group A[35.0% (14/40) vs.15.0% (6/40) and 30.0% (12/40) vs.10.0% (4/40)],and there was significant difference between two groups (P ＜ 0.05 ).Conclusion Oxycodon hydrochloride controlled-release tablets combined with gabapentin is effective and safe for the management of painful diabetic neuropathy.

ObjectiveTo investigate the effects of two health education models on the psychology and nutrition of patients waiting for cadaveric renal transplantation.Methods A total of 125 patients waiting for cadaveric renal transplantations were involved in our study.They were diagnosed with chronic renal failure in our hospital during September 1, 2009 to August 30, 2010.The patients were randomly divided into control group (n = 62) and observational group(n = 63).Patients in the control group received traditional health education with routine preoperative education during hospitalization.In the observational group, full-time nurses assessed the nutrition status of each patient and monitored the data. The observational patients were followed up and were given dietary guidance and knowledge of transplantation.Various kinds of education formats were adopted in observational group to provide communication opportunities between patients and surgeons in charge as well as patients who underwent transplantation.Psychological testings of patients in both groups were tested by self-rating anxiety scale (SAS) and self-rating depression scale (SDS) before and after the health education.Triceps skinfold thickness (TSF), mid-arm muscle circumference (MAMC) and biochemistry index were also tested.Psychological and nutritional status of patients in the two groups was compared.ResultsThere were no significant differences in scores of the SAS, SDS, TSF, Hb, and albumin (Alb) between the two groups (all P＞0.05) before health education.After health education, SAS and SDS in observational group were lower than those in the control group (40.02 ±9.05 vs 47.05 ± 10.32,42.70 ± 10.01 vs 50.83 ± 10.12; both P＜0.01).Both TSF and Hb were elevated after education (P ＜ 0.001 or 0.05).Alb was significantly elevated in the observational group [(35.67 ±6.19)g/L vs (37.48 ±5.09)g/L, P ＜0.01].ConclusionComprehensive and various health education methods can significantly alleviate mental stress and improve nutrition of the patients waiting for kidney transplantation, which is helpful for patients facing disease positively and having a better quality of