1.Evaluation of combined detection of antibodies specific for syphilis
Yahui SHE ; Yuanyuan HAN ; Guangting ZHOU ; Fangyan JIA
International Journal of Laboratory Medicine 2015;(3):324-325
Objective To evaluate ELISA combined with chemiluminescence in the detection of syphilis specific antibodies. Methods 628 patients who came to our hospital and treated in dermatology department were recruited in the study from January 2014 to April 2014.Patients′serum samples were detected by using ELISA firstly,and then the samples,the test rusults of which were within the recheck standard range(0.5
2. Determination of N-methyl-2-pyrrolidone in workplace air by solvent desorption-gas chromatography
Ruibo MENG ; Weifeng RONG ; Jiawen HU ; Jiaheng HE ; Zidan YAN ; Yuanyuan SHE ; Banghua WU
China Occupational Medicine 2019;46(02):229-232
OBJECTIVE: To establish a method for determination of N-methyl-2-pyrrolidone(NMP) in workplace air by solvent desorption-gas chromatography. METHODS: NMP in the workplace air was collected with activated carbon tube and desorbed with methanol ∶dichloromethane(5 ∶95, V/V). After desorption, it was separated by capillary column separation, and detected by flame ionization detector. RESULTS: The good linear concentration of NMP was 0.51-4 108.00 mg/L. The correlation coefficient was 0.999 9. The detection limit was 0.08 mg/L(calculated by 3 times of standard deviation). The average desorption efficiency was 101.68%-103.44%. The within-run and between-run relative standard deviations were 1.94%-3.97% and 0.97%-2.26%, respectively. The sample could be stored at room temperature for at least 14 days. CONCLUSION: The developed method is suitable for the determination of NMP in workplace air.
3. Simultaneous determination of three chlorobenzene compounds in workplace air by portable gas chromatography-mass spectrometry
Jiaheng HE ; Yuanyuan SHE ; Guanlin CHEN ; Weifeng RONG ; Ruibo MENG ; Jiawen HU ; Banghua WU
China Occupational Medicine 2020;47(03):339-342
OBJECTIVE: To establish a method for simultaneous determination of chlorobenzene, p-dichlorobenzene and o-dichlorobenzene in workplace air by portable gas chromatography-mass spectrometry(GC-MS). METHODS: The portable GC-MS heat tracing sampling probe was used for sampling. Samples were separated with LTM DB-5 MS rapid chromatographic column. The qualitative analysis was based on retention times and characteristic ions, and the quantification was based on standard curves. RESULTS: The linear correlation coefficient of this method was higher than 0.999 6. The minimum detectable concentrations were 0.03, 0.03 and 0.06 mg/m~3, and the minimum quantification concentrations were 0.10, 0.10 and 0.20 mg/m~3. The recovery rates were 84.68%-92.61%, 86.27%-93.92% and 82.31%-92.36% respectively for three chlorobenzenes compounds. The within-run relative standard deviations(RSD) were 8.51%-9.34%, 7.93%-9.19%, 5.47%-7.48% respectively for three chlorobenzenes compounds, the between-run RSD were 7.29%-9.73%, 8.08%-10.04% and 5.19%-5.98% respectively for three chlorobenzenes compounds. CONCLUSION: The portable GC-MS could be used for qualitative and quantitative detection of three chlorobenzenes compounds in workplace air.
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