1.Evaluation of combined detection of antibodies specific for syphilis
Yahui SHE ; Yuanyuan HAN ; Guangting ZHOU ; Fangyan JIA
International Journal of Laboratory Medicine 2015;(3):324-325
Objective To evaluate ELISA combined with chemiluminescence in the detection of syphilis specific antibodies. Methods 628 patients who came to our hospital and treated in dermatology department were recruited in the study from January 2014 to April 2014.Patients′serum samples were detected by using ELISA firstly,and then the samples,the test rusults of which were within the recheck standard range(0.5
2.Application of dynamic case scenario simulation in emergency ability training of new nurses in Department of Neurosurgery
Aifeng WANG ; Yuanyuan ZHANG ; Jinmei CHENG ; Weijie WANG ; Lianshu DING ; Fei SHE ; Chunxia SUN
Chinese Journal of Modern Nursing 2017;23(32):4165-4169
Objective To explore the application effect of dynamic case scenario simulation in emergency ability training of new nurses in Department of Neurosurgery.Methods A total of 28 nurses newly recruited in 2015 and 2016 to Department of Neurosurgery, Huai'an First People's Hospital, Nanjing Medical University were selected. According to time of joining the department, the 15 nurses recruited in 2015 were selected as control group, and the 13 nurses enrolled in 2016 as observation group. Nurses in the control group were trained by stages, i.e. technique training after theory training. On the basis of above mentioned training stages, nurses in the observation group were trained in simulated scenario, with 1 dynamic case added to each training stage. Emergency management ability, self evaluation of the nurses and patients' satisfaction after the training were compared between the two groups.Results After the training, scores of ability in disease observation, emergency cooperation and first-aid operation of new nurses in observation group were (27.41±1.32), (29.59±1.06) and (38.55±1.58), all higher than those in the control group (t=7.24, 12.26, 10.07;P<0.01). It was shown in results of self-evaluation that, ability to link theory with reality, to arouse subjective initiative and participation of nurses in the observation group were higher than those in the control group (P<0.05). Satisfaction of patients towards nurses in the observation group was 96.15%, higher than 86.00% in the control group (χ2=8.524,P<0.01).Conclusions Trained with method of dynamic case scenario simulation, emergency response ability of new nurses can be improved rapidly in a short time. Also, patients are more satisfied with the nursing. This training management method is worth promoting.
3. Determination of N-methyl-2-pyrrolidone in workplace air by solvent desorption-gas chromatography
Ruibo MENG ; Weifeng RONG ; Jiawen HU ; Jiaheng HE ; Zidan YAN ; Yuanyuan SHE ; Banghua WU
China Occupational Medicine 2019;46(02):229-232
OBJECTIVE: To establish a method for determination of N-methyl-2-pyrrolidone(NMP) in workplace air by solvent desorption-gas chromatography. METHODS: NMP in the workplace air was collected with activated carbon tube and desorbed with methanol ∶dichloromethane(5 ∶95, V/V). After desorption, it was separated by capillary column separation, and detected by flame ionization detector. RESULTS: The good linear concentration of NMP was 0.51-4 108.00 mg/L. The correlation coefficient was 0.999 9. The detection limit was 0.08 mg/L(calculated by 3 times of standard deviation). The average desorption efficiency was 101.68%-103.44%. The within-run and between-run relative standard deviations were 1.94%-3.97% and 0.97%-2.26%, respectively. The sample could be stored at room temperature for at least 14 days. CONCLUSION: The developed method is suitable for the determination of NMP in workplace air.
4. Simultaneous determination of three chlorobenzene compounds in workplace air by portable gas chromatography-mass spectrometry
Jiaheng HE ; Yuanyuan SHE ; Guanlin CHEN ; Weifeng RONG ; Ruibo MENG ; Jiawen HU ; Banghua WU
China Occupational Medicine 2020;47(03):339-342
OBJECTIVE: To establish a method for simultaneous determination of chlorobenzene, p-dichlorobenzene and o-dichlorobenzene in workplace air by portable gas chromatography-mass spectrometry(GC-MS). METHODS: The portable GC-MS heat tracing sampling probe was used for sampling. Samples were separated with LTM DB-5 MS rapid chromatographic column. The qualitative analysis was based on retention times and characteristic ions, and the quantification was based on standard curves. RESULTS: The linear correlation coefficient of this method was higher than 0.999 6. The minimum detectable concentrations were 0.03, 0.03 and 0.06 mg/m~3, and the minimum quantification concentrations were 0.10, 0.10 and 0.20 mg/m~3. The recovery rates were 84.68%-92.61%, 86.27%-93.92% and 82.31%-92.36% respectively for three chlorobenzenes compounds. The within-run relative standard deviations(RSD) were 8.51%-9.34%, 7.93%-9.19%, 5.47%-7.48% respectively for three chlorobenzenes compounds, the between-run RSD were 7.29%-9.73%, 8.08%-10.04% and 5.19%-5.98% respectively for three chlorobenzenes compounds. CONCLUSION: The portable GC-MS could be used for qualitative and quantitative detection of three chlorobenzenes compounds in workplace air.