Objective To construct the prokaryotic expression plasmid of HHV-8 fusion antigen for diagnosis of HHV-8 infec-tion .Methods The combined fragment ORF59 ,ORF65 and K8 .1 by fusion PCR was integrated into pQE-80L and transfected into E .coli DH5α.Fusion protein was induced to express by IPTG .SDS-PAGE and Western blot were employed to detect the fusion protein .Fusion protein was used to detect serum of blood donors .Results The combined plasmid pQE-80L-ORF59-ORF65-K8 .1 was constructed successfully after verifying by restriction enzyme digestion and sequencing .The fusion protein was about 24 KD and could be specific combined with HHV-8 positive serum .The fusion protein had the same result to detect HHV-8 with the HHV-8 ELISA kit .Conclusion Fusion protein we construct can be used as diagnosis antigen to detect HHV-8 of blood donors and common people .

OBJECTIVE: To investigate the postural stability after head-shaking in the normal young individuals. METHOD: Twenty-eight normal young individuals were taken the static posturography under 4 standing conditions including (T1) head center and eye open (EO), (T2) head center and eye closed, (T3) after head-shaking and EO, (T4) after head-shaking and EC. The sway velocity (SV) of center of pressure under feet in each testing condition was recorded as parameter and each test protocol was 30 s. RESULT: All examinee did not fall in each testing condition. (1) The SV increased (4.60 +/- 6.36) % after head-shaking in the EO condition, while the (8.86 +/- 8.27) % was demonstrated in the EC condition. There was significant difference between these two degrees of increase (t = 2.759, P < 0.01). (2) In static head orientation, the SV of EC (T2) increased (9.83 +/- 5.10)% compared with the SV of EO condition (T1). However, the SV of EC (T4) increased (14.05 +/- 6.89) % compared with the SV of EO condition (T3) after head-shaking. And there was significant difference between these two degrees of increase (t = 2.772, P < 0.01). CONCLUSION The stimuli of head-shaking that interrupted input from vestibular end organ can affect the function of vestibulospinal reflex in healthy young individual, especially when the visual input was interrupted simultaneously. In order to keeping balance after head-shaking, there was a complex mechanism sensory reorganization even in the normal young subject.
Female ; Head ; Humans ; Male ; Postural Balance ; Reflex, Vestibulo-Ocular ; physiology ; Young Adult

Objective:To reveal the regular pattern characteristics of different diseases with the same treatment in the most common diseases with blood stasis syndrome; To provide reference for the clinical treatment of blood stasis syndrome and the development of new drugs.Methods:RCTs of blood stasis syndrome were retrieved from CNKI, Chongqing VIP, Wanfang Data, SinoMed, and China Medical Journal Full-text Database from the establishment of the databases to December 31, 2022. Diseases, accompanied symptoms, prescriptions and medicines were extracted. The diseases with the highest frequency among the three disease systems with the highest frequency were collected, and their medication characteristics and prescription rules were analyzed using frequency statistics and association rules Apriori algorithm. The core prescriptions of blood stasis syndrome of three kinds of diseases were excavated and their network similarity was analyzed.Results:A total of 2 052 articles were included. Stable coronary heart disease, ischemic stroke and DN were more common diseases with blood stasis syndrome. The common drugs for the three diseases were Chuanxiong Rhizoma, Carthami Flos, Persicae Semen, Angelicae Sinensis Radix. The core prescription of stable coronary heart disease was Persicae Semen- Carthami Flos- Chuanxiong Rhizoma- Angelicae Sinensis Radix- Paeoniae Radix Rubra; the core prescription of ischemic stroke is Buyang Huanwu Decoction; the core prescription of DN was Persicae Semen- Carthami Flos- Chuanxiong Rhizoma- Angelicae Sinensis Radix- Cornus Officinalis- Dioscoreae Rhizoma- Astragali Radix. The similarity between stable coronary heart disease and ischemic stroke core prescription network was 0.35, the similarity between ischemic stroke and DN core prescription network was 0.29, and the similarity between stable coronary heart disease and DN core prescription network was 0.26. Conclusions:The theory of "different diseases with the same treatment" can profoundly guide clinical practice. The core medicines of blood stasis syndrome are Persicae Semen, Carthami Flos, Angelicae Sinensis Radix, and Chuanxiong Rhizoma. On this basis, combined with different diseases and syndromes to make changes of adding and subtracting.