〔Abstract〕 The paper analyzes problems existing in the current course of medical literature retrieval and proposes suggestions for re -forming the teaching contents and methods , including the integration of multimedia teaching and online class , integration of class teaching and practical operation , and integration of multiple teaching methods such as case teaching method , etc.It aims to effectively improve the teaching quality of medical literature retrieval and realize the objectives of cultivating medical students 'information literacy .

Objective To analyze the therapeutic effect of lidocaine in cervical cancer postoperative urinary retention.Methods Sixty-five patients with cervical cancer postoperative urinary retention were divided into two groups by random digits table method:treatment group 32 cases,immediately after the urethral catheter to infiltrate 1 ml of 2％ lidocaine cotton ball placed urethral opening more than 20 minutes again urine; control group 33 cases,routine for patients with urethral catheter.The incidence of urinary retention and adverse drug reactions were compared between two groups.Results Treatment group residual urine volume was(53 ± 22) ml,1 case(3.12％,1/32) of urinary retention,control group was (163 ± 32) ml and 8 cases (24.24％,8/33),there was significant difference (P ＜ 0.05).Conclusion Lidocaine for cervical cancer postoperative urinary retention has good prevention effect,can be popularized in clinical application.

Mitochondrial disease is a set of multi-systematic and heterogeneous disease which invades in the central nervous system and muscles tissue adequately,mainly refers to the genetic defects that causes mitochondrial structure and function defect.Mitochondrial disease occupies an important position in the human genetics.At present,the research of mitochondrial disease and the related genetic mutation has become the focus in the genetics.This review is to discuss the mitochondrial disease epidemiology,molecular biology and genetics,clinical manifestations and diagnosis.

Objective To compare the efficacy of lamivudine and telbivudine combined with hepatitis B vaccine in blocking vertical transmission of hepatitis B virus (HBV) in pregnant women with HBV infection during late stage of pregnancy.Methods From January 2010 to December 2013,195 pregnant women with HBV infection who received prenatal examination and institutional delivery were enrolled and randomized into three groups based on random number table method,lamivudine group,telbivudine group and control group,65 cases in each group.The subjects in lamivudine group and telbivudine group orally took lamivudine 100 mg/d and telbivudine 600 mg/d respectively,from the 28th week of gestation to 1 month after delivery.The primary end point was HBsAg positivity of infants at 12 months of age.Results Serum lgHBV DNA level of mothers in lamivudine group was 3.7 ± 1.2,and in telbivudine group was 3.6 ± 1.5,in control group was 8.4 ± 1.7.The serum lgHBV DNA level of mothers in lamivudine group and telbivudine group was significantly lower than that in control group (P ＜ 0.01).HBsAg positivity rate of infants at 12 months of age in lamivudine group was 3.1％(2/65),in telbivudine group was 4.6％(3/65),and in control group was 15.4％ (10/65).HBsAg positivity rate of infants at 12 months of age in lamivudine group and telbivudine group was significantly lower than that in control group (P ＜ 0.05).Conclusion Lamivudine and telbivudine are both effective in preventing vertical transmission of HBV for pregnant women with HBV infection in late stage of pregnancy,and further clinical study is required.

Hand,foot and mouth disease(HFMD) is a common acute infectious disease in children caused by intestinal viruses.The severe HFMD,mostly caused by enterovirus 71,can lead to meningitis,encephalitis,encephalomyelitis,pulmonaryedema,irculation disorder and high fatality.To investigate the research progress of pathogenesis,hysiopathology,immunoassay and diagnosis of severe HFMD can provide references to the clinical prevention and treatment.

To investigate the protective effects and possible mechanism of pomegranate flowers polyphenols (PFP) on liver function of rats with diabetes combining non-alcoholic fat liver diseases, diabetes combining nonalcoholic fat liver disease model rats were established with high calorie feeding and small dose intraperitoneal injection of streptozotocin (STZ). Model rats were randomly divided into: model group, metformin group, pomegranate flowers polyphenols low, medium and high dose group (75, 150 and 300 mg x kg(-1)). After four weeks treatment, the levels of FPG, blood fat profiles and serum insulin, ALT, AST levels, SOD and MDA in the liver and serum separately were analyzed with biochemical methods. Paraoxonase (PON1 and PON3) mRNA and protein expression in liver were checked by RT-PCR and immunohistochemical method. Pathological changes of the liver were observed. FPG, IRI, non-HDL-C and transaminase significantly reduced and HDL-C raised in the each PFP dose group; Furthermore, compared with model group, fat drops in liver cells significantly reduced, antioxidant ability enhanced, PON1 mRNA and protein expression level in liver increased significantly. The protective effects of PFP against diabetes combining non-alcoholic fat liver diseases rats might through the increase liver PON1 mRNA and protein expression further enhanced the body antioxidant capacity and reduced IRI so as to ameliorate the rat hepatic steatosis.

Bioethics is an interdisciplinary subject that initiated in the 1970s. It concerns about negative effects brought up by modern biotechnology and its revolutionary application and consequence. This essay summarizes the current research situation in China, the disputes on two of the major bioethics issues-genetics and cloning, makes a brief international comparison of public policies different countries take on bioethics issues, and indicates what Chinese government should pay more attention to in the policymaking process on bioethics: to confront the disputes, to emphasize the public participation, and enhance the transparency of public policymaking process.

Objective To investigate the epidemic situation of brucellosis in Yingkou City of Liaoning Province,and to provide a basis for making prevention and control measures.Methods Surveillance data of human brucellosis in Yingkou City from 2005 to 2014 were collected throng the China information system for disease control and prevention.The method of retrospective data analysis was used to analyze the distributions of region,time,age,sex,and occupation distribution of brucellosis.Results A total of 348 cases were infected with brucellosis in Yingkou City from 2005 to 2014,268 males and 80 females,and the sex ratio was 3.35:1.00.The age was mainly concentrated in 35-＜ 65 years old,a total of 251 cases,accounting for 72.13％ (251/348) of the total number of cases.Farmer was the main occupation of the patients,295 cases,accounting for 84.77％ (295/348) of the total number of cases.The city's lowest incidence was in 2011,0.340/100 000,the highest in 2014,3.343/100 000.All counties (districts or cities) had reported the epidemic,Gaizhou was the highest,a total of 225 cases,accounting for 64.66％ (225/348) of the total number of cases.The onset time focused on April-September each year,249cases,accounting for 71.55％ (249/348) of the total number of cases.Conclusions The epidemic of brucellosis in Yingkou City is in a spreading trend.Health education and inter-departments cooperation are in need in order to control the spreading of the epidemic.

Objective To study the clinical effect of sequential therapy by intravenous fat emulsion and hemoperfusion in patients with severe organophosphorus pesticide poisoning. Methods Seventy-two patients with severe organophosphorus pesticide poisoning were selected, and the patients were divided into sequential therapy group (36 cases) and conventional therapy group (36 cases) by random digits table method. All the patients were given conventional therapy including gastrolavage, purgation, fluid infusion, atropine, pralidoxime chloride, anti- infection, breath support and hemoperfusion. The patients in sequential therapy group were given intravenous fat emulsion before hemoperfusion. The clinical effect was compared between 2 groups. Results There were no statistical differences in the serum cholinesterase activity on admission and in the first day after treatment between 2 groups (P>0.05). The serum cholinesterase activities in the second, third and seventh day after treatment in sequential therapy group were significantly higher than those in conventional therapy group: (456.3 ± 45.6) U/L vs. (391.3 ± 39.8) U/L, (862.3 ± 96.2) U/L vs. (614.7 ± 78.5) U/L and (1950.9 ± 366.7) U/L vs. (1543.6 ± 285.9) U/L, and there were statistical differences (P<0.05). The total doses of atropine applied and length of stay in sequential therapy group were significantly less than those in conventional therapy group: (85.4 ± 36.2) mg vs. (104.7 ± 45.3) mg and (10.5 ± 4.2) d vs. (13.6 ± 6.2) d, and there were statistical differences (P<0.05). The recovery rate in sequential therapy group was significantly higher than that in conventional therapy group:97.2%(36/36) vs. 80.6%(29/36), and there was statistical difference (P<0.05). Conclusions Sequential therapy by intravenous fat emulsion and hemoperfusion can improve the serum cholinesterase activity, and decrease the total dose of atropine in patients with severe organophosphate pesticide poisoning.

Objective To observe the curative effect of acupuncture point bloodletting,flash tank combined with ultrashort wave auxiliary in the treatment of infantile cough.Methods 102 children with cough were selected and randomly divided into control group (50 cases)and observation group (52 cases).The control group adopted general anti -infection,reduce phlegm,the method of atomizing inhalation.The observation group received control method based on the acupuncture point thorn bloodletting,cans,combined with ultrashort wave.The clinical curative effect was compared in the two groups.Results In the treatment group,34 cases got cure,improvement in 17 cases, 1 case had no effect,the cure rate was 65.3%,total effective rate was 98.0%,without efficiency 1.9%.In the control group,18 cases got cure,improvement in 23 cases,9 cases of invalid,and the cure rate was 36.0%,the total effective rate was 82.0%,without efficiency was 18.0%.The total effective rate between the two groups had statistically significant difference (χ2 =4.105,P <0.05).Conclusion Acupuncture point bloodletting,flash tank combined with ultrashort wave auxiliary in the treatment of infantile cough can obviously improve the clinical effect.