Bcl-2 family have dual-regulating effects on cell apoptosis mediated by mitoehondrion. The ratio of pro-apoptosis members and anti-apoptosis members closely correlates with tumorigenesis, drug-resist-ance and prognosis. Therefore,Bcl-2 family become important targets in tumor biotherapy. Many strategies have been applied to tumors treatment targeting Bcl-2 family,such as some biological treatment,short peptides and organic small molecules.

Objective To investigate the epidemic situation of brucellosis in Yingkou City of Liaoning Province,and to provide a basis for making prevention and control measures.Methods Surveillance data of human brucellosis in Yingkou City from 2005 to 2014 were collected throng the China information system for disease control and prevention.The method of retrospective data analysis was used to analyze the distributions of region,time,age,sex,and occupation distribution of brucellosis.Results A total of 348 cases were infected with brucellosis in Yingkou City from 2005 to 2014,268 males and 80 females,and the sex ratio was 3.35:1.00.The age was mainly concentrated in 35-＜ 65 years old,a total of 251 cases,accounting for 72.13％ (251/348) of the total number of cases.Farmer was the main occupation of the patients,295 cases,accounting for 84.77％ (295/348) of the total number of cases.The city's lowest incidence was in 2011,0.340/100 000,the highest in 2014,3.343/100 000.All counties (districts or cities) had reported the epidemic,Gaizhou was the highest,a total of 225 cases,accounting for 64.66％ (225/348) of the total number of cases.The onset time focused on April-September each year,249cases,accounting for 71.55％ (249/348) of the total number of cases.Conclusions The epidemic of brucellosis in Yingkou City is in a spreading trend.Health education and inter-departments cooperation are in need in order to control the spreading of the epidemic.

Hand,foot and mouth disease(HFMD) is a common acute infectious disease in children caused by intestinal viruses.The severe HFMD,mostly caused by enterovirus 71,can lead to meningitis,encephalitis,encephalomyelitis,pulmonaryedema,irculation disorder and high fatality.To investigate the research progress of pathogenesis,hysiopathology,immunoassay and diagnosis of severe HFMD can provide references to the clinical prevention and treatment.

Autism is a series of extensive and severe neuronal disorders.Autism has renamed autism spectrum disorder(ASD)in the United States of the Diagnosis and Classification Manual Fifth Edition in May,2013.Abnormal neuroimmunological pathogenesis research of ASD in recent years is reviewed in this paper.Studies have found that children with ASD have abnormal proinflammatory factor,neurotransmitter and the emergence of a brain protein antibody.

To investigate the protective effects and possible mechanism of pomegranate flowers polyphenols (PFP) on liver function of rats with diabetes combining non-alcoholic fat liver diseases, diabetes combining nonalcoholic fat liver disease model rats were established with high calorie feeding and small dose intraperitoneal injection of streptozotocin (STZ). Model rats were randomly divided into: model group, metformin group, pomegranate flowers polyphenols low, medium and high dose group (75, 150 and 300 mg x kg(-1)). After four weeks treatment, the levels of FPG, blood fat profiles and serum insulin, ALT, AST levels, SOD and MDA in the liver and serum separately were analyzed with biochemical methods. Paraoxonase (PON1 and PON3) mRNA and protein expression in liver were checked by RT-PCR and immunohistochemical method. Pathological changes of the liver were observed. FPG, IRI, non-HDL-C and transaminase significantly reduced and HDL-C raised in the each PFP dose group; Furthermore, compared with model group, fat drops in liver cells significantly reduced, antioxidant ability enhanced, PON1 mRNA and protein expression level in liver increased significantly. The protective effects of PFP against diabetes combining non-alcoholic fat liver diseases rats might through the increase liver PON1 mRNA and protein expression further enhanced the body antioxidant capacity and reduced IRI so as to ameliorate the rat hepatic steatosis.

At 4Hz and 16Hz temporal frequencies and at a range of 3—37.8 cycles per degree (cpd) of spatial frequency which were divide into 12 grades, an instrument with a flickering sinusoid grate was used to measure the laser retina-visual cortex spatiotemporal visual modulation transfer function (S-TMTF) in patients with glaucoma. There were 34 cases (65 eyes) with early primary open-angle glaucoma (POAG), 18 cases (31 eyes) with moderate or late glaucoma and 19 cases (37 eyes) with suspect glaucoma (SG). In 6 months. 16 patients of SG were followed. The results showed that in patients with early POAG, abnormal S-TMTF values might occur in low frequency region, the abnormal values tended to be general in the middle frequency region, and in the high frequency region all the S-TMTF values were significantly lower than that of the control. In cases with SG, lowering of S-TMTF was seen in many frequency points above 9.5 cpd, but did not found in regions below 9.5 cpd. The S-TMTF values at 16 Hz were lower than that at 4 Hz in each corresponding spatial frequency, and with aggravation of the disease, the greatest discrepancy value appeared to be shifting from high to low frequency points. In the follow-up period, an early glaucomatous visual field change occurred in a female case with ocular hypertension. It is demonstrated that the S-TMTF value is of high sensitivity for diagnosis of POAG, but it is difficult to screen the specific diagnostic frequency point and its critical value.

"Correspondence between nature and human" is the basic thought of ancient Chinese philosophy. It had different meanings at different historical period. In Shang and Zhou dynasty, nature and human were combined together by sorcery; in Han dynasty, "qi" became the link of nature and human; and in Song and Ming dynasty, the link turned to the "Li".The concept of "correspondence between nature and human" improved the formation TCM theory and influenced its development for all the time.

Objective To analyze the therapeutic effect of lidocaine in cervical cancer postoperative urinary retention.Methods Sixty-five patients with cervical cancer postoperative urinary retention were divided into two groups by random digits table method:treatment group 32 cases,immediately after the urethral catheter to infiltrate 1 ml of 2％ lidocaine cotton ball placed urethral opening more than 20 minutes again urine; control group 33 cases,routine for patients with urethral catheter.The incidence of urinary retention and adverse drug reactions were compared between two groups.Results Treatment group residual urine volume was(53 ± 22) ml,1 case(3.12％,1/32) of urinary retention,control group was (163 ± 32) ml and 8 cases (24.24％,8/33),there was significant difference (P ＜ 0.05).Conclusion Lidocaine for cervical cancer postoperative urinary retention has good prevention effect,can be popularized in clinical application.

〔Abstract〕 The paper analyzes problems existing in the current course of medical literature retrieval and proposes suggestions for re -forming the teaching contents and methods , including the integration of multimedia teaching and online class , integration of class teaching and practical operation , and integration of multiple teaching methods such as case teaching method , etc.It aims to effectively improve the teaching quality of medical literature retrieval and realize the objectives of cultivating medical students 'information literacy .

Objective To compare the efficacy of lamivudine and telbivudine combined with hepatitis B vaccine in blocking vertical transmission of hepatitis B virus (HBV) in pregnant women with HBV infection during late stage of pregnancy.Methods From January 2010 to December 2013,195 pregnant women with HBV infection who received prenatal examination and institutional delivery were enrolled and randomized into three groups based on random number table method,lamivudine group,telbivudine group and control group,65 cases in each group.The subjects in lamivudine group and telbivudine group orally took lamivudine 100 mg/d and telbivudine 600 mg/d respectively,from the 28th week of gestation to 1 month after delivery.The primary end point was HBsAg positivity of infants at 12 months of age.Results Serum lgHBV DNA level of mothers in lamivudine group was 3.7 ± 1.2,and in telbivudine group was 3.6 ± 1.5,in control group was 8.4 ± 1.7.The serum lgHBV DNA level of mothers in lamivudine group and telbivudine group was significantly lower than that in control group (P ＜ 0.01).HBsAg positivity rate of infants at 12 months of age in lamivudine group was 3.1％(2/65),in telbivudine group was 4.6％(3/65),and in control group was 15.4％ (10/65).HBsAg positivity rate of infants at 12 months of age in lamivudine group and telbivudine group was significantly lower than that in control group (P ＜ 0.05).Conclusion Lamivudine and telbivudine are both effective in preventing vertical transmission of HBV for pregnant women with HBV infection in late stage of pregnancy,and further clinical study is required.