Objective:To explore the implicit attitude and explicit attitude toward death of college students,and the relationship between the two kinds of attitude.Methods:Totally 65 college studems participated in this study.The Single Category Implicit Association Test (SC-IAT,including cognition,affection and behavior) was used to exam the implicit attitude toward death of undergraduate students.The Death Attitude Profile-Revised (DAP-R,including five dimensions,i.e.,fear of death,death avoidance,death acceptance,escape acceptance,and neutral acceptance) was used to measure the explicit attitude.Results:Data of the SC-IAT indicated that the differences between the mean reaction time of the incompatible task and compatible in all three dimensions and the total SC-IAT were significant (Ps ＜0.01),and both the D scores of the total SC-IAT and three dimensions were larger than zero.The mean score of neutral acceptance was the highest in the five factors of SC-IAT.Relationships between the implicit effect of SC-IAT and the Mean scores of the DAP-R's five factors were not significant(Ps ＞ 0.05).Conclusion:The implicit attitude toward death of college students is negative,the explicit attitude toward death of college students is neutral.The implicit and explicit attitude toward death of college students is incongruent,and they are relatively independent.

Avian influenza virus H6N1 subtype has been circulating in aquatic bird and terrestrial bird,and is the most frequently detected subtype of influenza A virus in those hosts.Genetic analysis results suggested that this virus might be the progenitor of the highly pathogenic avian influenza H5N1 virus,as seven of eight gene segments of those viruses had a common source.As continuing evolution of H6N1,it could spread across species barriers to mammals and had strong infection ability in mice,swine and ferrets.Serological epidemiological survey showed that a few people were positive for H6 avian influenza virus antibody and then the world's first human infection with influenza virus H6N1 subtype was reported in May 2013.Therefore,with the expansion of the host range of the virus,gene mutation and gene reassortment of H6N1 virus can occur in these hosts,and then it may evolve into novel variant strain with infected human potential.

According to the theory of microscopic system reform in higher education, a total of 927 college students of different majors from Beijing University of Chinese Medicine were investigated by using SIMPP method. It indicated that through promoting the study of self-prepared, learning state and learning effect, students can enhance their learning ability of the admission acceptance. From the perspective of teachers, the students' self-study ability can be fully mobilized using individual person-alized teaching method. Then, the initiative of university education in the teaching and learning can be improved. Ultimately, the efficiency of student learning can be improved.

Objectives To explore the clinical diagnosis and treatment of Dieulafoy disease in children. Method The clinical features, endoscopic features and treatment of Dieulafoy disease in a child were reviewed. Results The 2-year-5-month old girl was admitted due to hematemesis for 7 hours. She was diagnosed of Dieulafoy disease by the typical endoscopic appearance. Gastroscopy showed that the lesion was located in gastric angle which was the predilection position of Dieulafoy disease. The small red blood vessels in the central part of the erosion area was exposed on the mucosal surface. The high frequency electrocoagulation under gastroscope was performed and effect was definite. Conclusion Dieulafoy disease is rare in children and lacks obvious clinical features. Endoscopic treatment has definite effect with little trauma and is the first choice of treatment.

To study the effects of Roudoukou-8 San against hydrogen peroxide-induced cardiomyocyte in neonatal rats and to explore its mechanism. Cardiomyocytes were isolated and cultivated by neonatal rats and the injure models were established by H2O2. Methyl thiazolyl tetrazolium(MTT)assay was used to detect the protective effects of Roudoukou-8 San on H2O2-induced cardiomyocyte. The effects of Roudoukou-8 San on myocardium morphology were observed under inverted microscope. The contents of lactate dehydrogenase(LDH), creatine kinase(CK)and aspartate aminotransferase(AST)in cell culture medium were determined by Automatic biochemical instrument; The levels of malonydialdehyde(MDA), superoxide dismutase(SOD)and NO in the cells were detected by kit method. The apoptotic morphology of cardiomyocytes was observed by Hoechst fluorescence staining. Cell apoptosis were measured by Annexin V and PI double staining and flow cytomety. 100μmol/L H2O2 for 2 h could cause about 50% of myocardial injury. In the inverted optical microscope, H2O2 model group showed increased cell gap, decreased cell count, cell cytoplasmic vacuoles and other obvious damage. Roudoukou-8 San protected cell from H2O2-induced morphlogical improved in different degrees, reduced the release of LDH, CK and AST content, reduced the content of MDA, NO in myocardial cells significantly and increased the activity of SOD. Roudoukou-8 San energy significantly inhibited H2O2 damage myocardial cell apoptosis. Our study suggested that Roudoukou-8 San can protect cardiomyocyte from H2O2-induced injury by improving the cell viability, reducing oxidative stress injury, inhibiting inflammatory reaction and inhibiting cell apoptosis.

Objective To investigate the changes and clinical significance of red blood cell distribution width(RDW)in peripheral blood of patients with adenomyosis.Methods A retrospective study was performed on 300 adenomyosis patients and 250 age-matched individuals who were admitted to the Maternal & Child Health Hospital of Guangxi Zhuang Autonomous Region from January 2020 to December 2022.Mann-Whitney U test was used to compare blood parameters between the patient group(including before and after treatment)and the healthy control group.The diagnostic efficacy of RDW in adenomyosis was evaluated by ROC.Spearman correlation method was used to analyze RDW's association with other blood inflammatory parameters.Logistic multivariate analysis was applied to analyze the independent risk factors for adenomyosis.Results Compared with healthy control group,RDW values were in patients with adenomyosis[14.60％(13.20％～18.65％)vs 13.00％(12.40％～13.80)％],and the difference was statistically significant(Z=11.396,P=0.001).In addition,the RDW levels of patients after treatment were reduced compared with healthy control group[14.10％(12.10％～15.90％)vs 14.60％(13.20％～18.65％)],and the difference was statistically significant(Z=8.339,P=0.001).Correlation analysis results showed that RDW was negatively correlated with hemoglobin,hematocrit,mean corpuscular volume and absolute lymphocyte count(r=-0.709,-0.614,-0.801,-0.131,all P＜0.05),but positively correlated with red blood cell,platelet and platelet lymphocyte ratio(r=0.184,0.439,0.406,all P＜0.05).ROC curve analysis showed that the sensitivity and specificity of RDW for diagnosing adenomyosis were 63.0％and 97.6％,the area under the curve was 0.782,95％confidence interval was 0.745～0.819,respectively.Logistic regression analysis showed that RDW was independently associated with adenomyosis(OR=0.755,95％CI=0.677～1.347,P=0.012).Conclusion RDW was elevated in patients with adenomyosis,suggesting that RDW may be a potential auxiliary diagnostic marker for adenomyosis.

Objective To observe the changes of laboratory blood indexes in patients with intrahepatic cholestasis of pregnancy(ICP),and analyze the value of blood inflammation indexes and liver function indexes in the diagnosis of ICP and the prediction of delivery mode.Methods A total of 251 patients diagnosed with ICP in this hospital from January 2021 to December 2022 were selected as the ICP group,and another 200 healthy pregnant women were selected as the control group.The patients with ICP were further divided into the severe ICP group(n=47)and the mild ICP group(n=204),the vaginal delivery group(n=113)and the cesarean section group(n=138)according to the severity of ICP and delivery mode.Mann-Whitney U test was used for comparison of parameters between groups,and Spearman method was used for correlation analy-sis.Receiver operating characteristic(ROC)curves were used to evaluate the efficacy of laboratory indicators in diagnosing ICP and predicting delivery mode.Results Neutrophil/lymphocyte ratio(NLR)[6.01(4.45,8.37)vs.3.36(4.12,3.51)]and aspartate transaminase(AST)level[20.00(16.00,33.00)U/L vs.15.00(13.00,18.00)U/L]in the ICP group were significantly higher than those in the control group(P＜0.05),and NLR in the severe ICP group was significantly higher than that in the mild ICP group[4.93(3.87,7.35)vs.4.14(3.12,5.17),P＜0.05].Correlation analysis showed that NLR was positively correlated with AST level(r=0.279,P＜0.001)and ICP severity(r=0.139,P=0.028)in patients with ICP.The area under ROC curve(AUC)of NLR combined with AST for ICP diagnosis was 0.882(95％CI:0.851-0.913).In ad-dition,cholinesterase(CHE)[6 020.00(5 499.50,6 703.50)U/L vs.5 341.50(4 651.75,6 259.25)U/L]and prealbumin(PA)[199.00(177.71,225.20)mg/Lvs.169.17(139.18,204.40)mg/L]levels in the va-ginal delivery group were significantly higher than those in the cesarean section group(P＜0.05),and the AUC of CHE combined with PA for predicting vaginal delivery in ICP patients was 0.727(95％CI:0.664-0.789).Conclusion NLR and AST have potential value in the diagnosis of ICP,and CHE and PA have poten-tial value in predicting delivery mode of ICP patients.

Bivalves are species-rich mollusks with prominent protective roles in coastal ecosystems.Across these ancient lineages,colony-founding larvae anchor themselves either by byssus produc-tion or by cemented attachment.The latter mode of sessile life is strongly molded by left-right shell asymmetry during larval development of Ostreoida oysters such as Crassostrea hongkongensis.Here,we sequenced the genome of C.hongkongensis in high resolution and compared it to reference bivalve genomes to unveil genomic determinants driving cemented attachment and shell asymmetry.Importantly,loss of the homeobox gene Antennapedia(Antp)and broad expansion of lineage-specific extracellular gene families are implicated in a shift from byssal to cemented attachment in bivalves.Comparative transcriptomic analysis shows a conspicuous divergence between left-right asymmetrical C.hongkongensis and symmetrical Pinctada fucata in their expression profiles.Especially,a couple of orthologous transcription factor genes and lineage-specific shell-related gene families including that encoding tyrosinases are elevated,and may cooperatively govern asymmet-rical shell formation in Ostreoida oysters.

Targeted point mutagenesis through homologous recombination has been widely used in genetic studies and holds considerable promise for repairing disease-causing mutations in patients. However, problems such as mosaicism and low mutagenesis efficiency continue to pose challenges to clinical application of such approaches. Recently, a base editor (BE) system built on cytidine (C) deaminase and CRISPR/Cas9 technology was developed as an alternative method for targeted point mutagenesis in plant, yeast, and human cells. Base editors convert C in the deamination window to thymidine (T) efficiently, however, it remains unclear whether targeted base editing in mouse embryos is feasible. In this report, we generated a modified high-fidelity version of base editor 2 (HF2-BE2), and investigated its base editing efficacy in mouse embryos. We found that HF2-BE2 could convert C to T efficiently, with up to 100% biallelic mutation efficiency in mouse embryos. Unlike BE3, HF2-BE2 could convert C to T on both the target and non-target strand, expanding the editing scope of base editors. Surprisingly, we found HF2-BE2 could also deaminate C that was proximal to the gRNA-binding region. Taken together, our work demonstrates the feasibility of generating point mutations in mouse by base editing, and underscores the need to carefully optimize base editing systems in order to eliminate proximal-site deamination.
APOBEC-1 Deaminase ; genetics ; metabolism ; Animals ; Bacterial Proteins ; genetics ; metabolism ; Base Sequence ; CRISPR-Associated Protein 9 ; CRISPR-Cas Systems ; Cytidine ; genetics ; metabolism ; Embryo Transfer ; Embryo, Mammalian ; Endonucleases ; genetics ; metabolism ; Gene Editing ; methods ; HEK293 Cells ; High-Throughput Nucleotide Sequencing ; Humans ; Mice ; Mice, Inbred C57BL ; Microinjections ; Plasmids ; chemistry ; metabolism ; Point Mutation ; RNA, Guide ; genetics ; metabolism ; Thymidine ; genetics ; metabolism ; Zygote ; growth & development ; metabolism ; transplantation

Adenine ; Animals ; Gene Editing ; Mice ; Zygote ; metabolism