Objective To achieve high throughput and high detection rate of circulating tumor cells(CTCs)in human peripheral blood,and to provide efficient and accurate early screening for cancer patients.Methods A microfluidic chip with the integration of sorting,enrichment and detection was designed,and CTCs at the single cell level were detected by fluorescence detection system to obtain the number of CTCs in samples.Results The peripheral blood samples after lysed red blood cells were used for 6 experiments.When the injection rate reached 0.2 mL/h,CTCs could reach the best detection rate of 78.6%,and the correlation coefficient within the group was above 0.8.Conclusion CTCs detection system can achieve high detection rate and has good reliability,which can provide a reliable reference for clinical research in related fields.