Objective To observe the clinical efficacy of acupoint application based on the formula ofBu Yang Huan Wu decoction in treating stroke due to qi deficiency and blood stagnation.Method Totally 109 patients with stroke due to qi deficiency and blood stagnation were randomized into a treatment group of 55 cases and a control group of 54 cases. In addition to the basic treatment, the treatment group was intervened by acupoint application based on formula ofBu Yang Huan Wu decoction at Dazhui (GV 14), Zusanli (ST 36), and Xuanzhong (GB ), while the control group received relevant health education. The symptom and syndrome score of traditional Chinese medicine (TCM), National Institute of Health Stroke Scale (NIHSS), and Barthel Index (BI) were observed before and after treatment, and the clinical efficacies and relapse rates were compared.Result The symptom and syndrome score of TCM, NIHSS score, and BI score were significantly different from that before treatment in both groups (P<0.01). The changes of the TCM symptom and syndrome score, NIHSS score, and BI score in the treatment group were significantly different from that in the control group (P<0.05). The total effective rate was 88.2% in the treatment group versus 69.2% in the control group, and the difference was statistically significant (P<0.05). Four cases in the treatment group got relapsed, accounting for 7.8%; 7 cases got relapsed in the control group, accounting for 13.5%. There was no significant difference in comparing the relapse rate between the two groups (P>0.05).Conclusion Acupoint application is an effective method in treating stroke due to qi deficiency and blood stagnation.

Objective To investigate the separation rate ,clinical distribution ,antibiotic susceptibility rate of Staphylococcus au‐reus from skin and soft tissue infections and provide the reference for clinical diagnosis and treatment .Methods Retrospective sta‐tistical analysis of 214 strains of Staphylococcus aureus from skin and soft tissue infections from September 2011 to August 2014 by Whonet 5 .6 .Results 1 228 pathogens were isolated from 2 152 cases of the secretion of operative and skin wound ,of which there were 214 strains of Staphylococcus aureus ,which accounted for 17 .43% ,48 strains of MRSA ,which accounted for 22 .43% ;Mainly distributed in bone surgery ,burns orthopaedic ,breast surgery ,with skin venereal division ,accounting for 40 .2% ,22 .3% ,12 .1% , 8 .4% respectively .The resistance rate of Staphylococcus aureus to penicillin ,erythromycin ,clindamycin were 96 .3% ,46 .7% , 37 .4% respectively ,high sensitivity to vancomysin ,linezolid ,fusidic acid ,mupirocin .Conclusion Surgical infection rates of SSTIs are high in our hospital .It should be according to SAU susceptibility analysis results to choose high sensitivity of the first and sec‐ond generation cephalosporins for treatment in clinic ,and vancomycin ,linezolid only in severe Staphylococcus aureus(including MR‐SA) infection to choose .

Objective To study the clinical characteristics of multi-drug resistance bacterias(MDROs) isolated from hospitalized patients in the Second People′s Hospital of Longgang District,to provide strategies for the prevention of MDROs infection.Methods The MDROs data of hospitalized patients from January 2015 to December 2016 were analyzed retrospectively.The multi-drug resistance incidence of each bacterias,each types of specimens and each clinical departments were analyzed and compared by SPSS16.0.Results A total of 104 strains of MDROs were isolated,and the top five bacteria were E.coli(32 strains,30.77%),coagulase negative staphylococcus(24 strains,23.08%),pseudomonas aeruginosa(16 strains,15.38%),staphylococcus aureus(10 strains,9.62%),kiebsiella pneumonia(10 strains,9.62%) respectively.There was significant difference in the multi-drug resistance incidence of each bacterias(χ2=20.62,P<0.05),the average incidence was 25.12%,and the top three incidence were E.coli(36.78%),pseudomonas aeruginosa(33.33%),coagulase negative staphylococcus(28.24%) respectively.There was significant difference in the multi-drug resistance positive rate of each types of specimens(χ2=43.68,P<0.05),the average positive rate was 5.84%,and the highest positive rate were wound secretion and pus(11.00%),followed by urine(8.25%).There was significant difference in the multi-drug resistance positive rate of each clinical departments(χ2=40.36,P<0.05),and the highest positive rate were in department of urinary surgery(12.63%),followed by department of gynaecology and obstetrics(11.16%).Conclusion E.coli coagulase negative staphylococcus and pseudomonas aeruginosa were mainly epidemic MDROs,and the MDROs are mainly distributed in urological surgery,obstetrics and gynecolog in this hospital.The occurrence of MDROs should be for the prevention and control strongly in the hospitalized patients with all kinds of trauma or diseases of urinary system and in the clinical department of urinary surgery,gynaecology and obstetrics.

Objective To explore the functional sensitivity ,maximum allowable dilution ,analytical measurement range and clin‐ical reportable rang of the serum alanine aminotransferase (ALT ) detected by Beckman Dxc800 automatic analyzer with the DiaSys diagnostic biochemistry reagent kits .Methods According to the EP6‐A document published in 2004 by American Clinical and La‐boratory Standards Institution (CLSI) ,these samples with equal interval ,proportional prepared with the low levels serum and the high levels serum and their concentration exceeding the range claimed by manufacture ,were detected twice each .The results were registered and analyzed by the SPSS19 .0 statistic software with polynomial regression analysis to determine the analytical measure ‐ment range .Five samples at the low concentration ,which were prepared previously ,were detected repeat at day interval .And the functional sensitivity was determined with combining the coefficient of variation and the average value .Three samples ,diluted to different dilution ratio with physiological saline ,were detected .The dilution recovery rate ,which was required in the range of 90%- 110% ,was calculated to establish the clinical reportable rang by combining the Functional Sensitivity .Results The analytical measurement range of the serum ALT detected by Beckman Dxc800 analyzer was 5 .50 - 847 .50 U /L ,the runctional sensitivity was 3 .698 U /L ,the maximum allowable dilution was 1 ∶ 8 and the clinical reportable rang was 3 .698 - 6 780 .0 U /L .Conclusion The analytical measurement range of the serum ALT detected by Beckman Dxc800 analyzer with the DiaSys diagnostic biochemistry rea‐gent kits ,was wider than that of being claimed by manufacture ,the clinical reportable rang could meet the need of clinic .

Objective To investigate the clinical application value of point-of-care combined detection of cTnI,MYO and CK-MB in the patients with acute myocardial infarction (AMI).Methods 74 cases of coronary syndrome were collected as the patients group,including 36 cases of AMI and 38 cases of unstable angina pectoris(UAP),and 40 individuals with healthy physical examina-tion were selected as the control group.The concentrations of cardiac marker cTnI,MYO and CK-MB in blood at different times were detected by using the point-of-care rapid immune quantitative method.Then the sensitivity and specificity for diagnosing AMI were compared for determining the best time of single detection and combined detection.Results The positive rates of cTnI,MYO and CK-MB within onset 2-12 h with the symptoms in the AMI group were higher than those in the control group and the UAP group,the difference had statistical significance (P <0.01).The combined detection of cTnI,MYO and CK-MB within onset 6-12 h with symptoms had the higher sensitivity and higher specificity for diagnosing AMI.The combined detection of cTnI and CK-MB within onset 12-24 h with symptoms could reach the optimal sensitivity and optimal specificity for the diagnosis.The sensitivity and specificity of cTnI within onset 24-72 h with symptoms for diagnosing AMI were 100.0% and 100.0% respectively.Conclu-sion The point-of-care combined detection of cTnI,MYO and CK-MB can conveniently and rapidly diagnose AMI.The sensitivty and specificity of various indexes in different time periods are different.Their combined detection can increase the diagnostic rate for AMI.

Objective To study on the relationship between the levels of homocysteine(HCY) ,folic acid and vitamin B12 and pregnancy-induced hypertension(PIH) in different pregnancies .Methods 539 pregnant women who registered for prenatal exami-nation of pregnant in the hospital were selected as research subjects .And there were 87 cases of PIH(PIH group) and 452 cases of normal pregnancy(normal pregnancy group) among them .The fasting blood samples were collected respectively in early pregnancy (8-10 weeks and 12-14 weeks of pregnancy) ,mid pregnancy(18 pregnancy weeks and 24 pregnancy weeks) ,and late pregnancy (30 pregnancy weeks and 36 pregnancy weeks) ,and the levels of HCY ,folic acid and vitamin B12 were measured .At the same time ,the supplements of folic acid and vitamin B12 and the incidence of PIH and birth defects were asked ,registered and checked . Results Compared with normal pregnancy group ,the serum HCY level of PIH group significantly increased in medium and late pregnancy periods (P<0 .05) ,and had no statistical significance in early pregnancy(P>0 .05) .In mid and late pregnancy periods , the serum HCY levels of PIH group and normal pregnant group negatively correlated with serum folic acid levels (r<0 ,P<0 .05) , and did not correlate with vitamin B12 levels (P>0 .05) .Conclusion In middle and late pregnancy periods ,if the serum HCY level of pregnant women increased ,the risk of PIH increased significantly .

BACKGROUNDTo explore the clinical significance of detection of T helper cell (Th1 and Th2) in patients with lung cancer and to provide a foundation for immunological treatment.

METHODSRIA and ELISA were used to detect the level of serum IL-2, IL-4, IL-6, IL-8 and TNF-a in 86 patients with lung cancer, 59 patients with benign pulmonary diseases and 45 healthy people. IL-2 and TNF-a were used to represent cytokines of Th1 type, and IL-4, IL-6 and IL-8 to represent cytokines of Th2 type.

RESULTSThe level of IL-2 [(24.6±12.0) μg/L]in cancer group was significantly lower than that in benign group [(71.1±25.4) μg/L] ( t =3.82, P < 0.01) and normal group [(69.3±19.5) μg/L]( t=2.76, P < 0.01), the level of IL-6 in cancer group [(0.13±0.04) μg/L] was significantly lower than that in normal group [(0.23±0.05) μg/L]( t= 3.39 , P < 0.01), but the levels of IL-4 [(254.2±78.0) μg/L], IL-8 [(0.49±0.16) μg/L], and TNF-a [( 2.76 ±1.12) μg/L] in cancer group were significantly higher than those in benign group [(63.6±18.6) μg/L, ( 0.36 ±0.18) μg/L, (0.96±0.20) μg/L respectively] and those in normal group [(60.9±19.6) μg/L, ( 0.35 ±0.07) μg/L, (0.93±0.19) μg/L respectively] ( t =4.10, 4.89, 3.76 respectively, all P < 0.01). No significant difference of IL-2, IL-4, IL-8 and TNF-a level was observed between benign group and normal group (all P > 0.05). The level of IL-6 in cancer group was similar to that in benign group [(0.15±0.04) μg/L] ( P > 0.05 ). The level of IL-6 in benign group was significantly lower than that in normal group [(0.23±0.05) μg/L] ( P > 0.05 ). There was no significant difference in these cytokines among lung cancer patients with different histological types and in different TNM stages.

CONCLUSIONST helper cell cytokines are out of balance in patients with lung cancer, and this may play a certain role in the pathogenesis of lung cancer. Correcting this immune malfunction may become an important method in lung cancer therapy.