Objective To predict the disease burden of kidney cancer and to provide basic information for etiology and control planning.Methods We retrieved incidence data of kidney cancer from 18 urban cancer registries from National Central Cancer Registry during ten years period from 1998 to 2007.Ageperiod-cohort Bayesian model was applied for modeling to predict kidney cancer incidence in urban China in 2008-2015.Results Between 1998 and 2007,the incidence of kidney cancer in urban registration areas kept increasing dramatically.Incidence for male raised from 3.12/100 000 in 1998 to 5.36/100 000 in 2007 and from 1.66/100 000 to 2.67/100 000 for female.Different models showed that the increase was mainly caused by a cohort effect (P ＜ 0.001).The predicted incidence rate of kidney cancer for the year 2015 is 9.93 per 100 000 in male and 4.54 per 100 000 in female.The number of new cases will rise to 52 259 in 2015,including 36 616 men and 15 643 women.Conclusions The burden of kidney cancer in urban areas would increase due to the effect of age and cohort.Kidney cancer will become one of the main cancers threatening people's health in urban areas in China.Etiology research and planning of prevention and control for kidney cancer should be enhanced.

Objective To detect the expression of gonadotropin-releasing hormone (GnRH) in cervical cancer and to explore the correlation.Methods A total of 68 patients with cervical squamous cell carcinoma and 45 patients with chronic cervicitis were selected.Immunohistochemistry and hybridization in situ were used to detect the expression of GnRH in cancer tissue and cervicitis.Protein and nucleic acid level were observed in patients with GnRH.Results In 45 patients with cervicitis, both GnRH and GnRH mRNA expressions were negative in cervical epithelial tissue, while in 68 cases of cervical squamous cell cancer, the positive expression of GnRH in cancer tissues was 82.36%, and positive expression rate of GnRH mRNA was 64.71%.There were significant differences in levels of GnRH and GnRH mRNA between patients with cervicitis and cervical squamous cell carcinoma (P<0.05).The qualitative expression rates of GnRH in different clinical stages of cervical cancer were 55.56% of Ib, 87.10% of IIa and 100% of IIb, and qualitative expression rates of GnRH mRNA in different clinical stages of cervical cancer were 38.89% of Ib, 70.97% of IIa and 84.21% of IIb.There were significant differences in GnRH and GnRH mRNA levels between patients with different clinical stages of cervical cancer (P<0.05).Conclusion There is a high level of gonadotropin-releasing hormone in cervical cancer, which may be involved in the development of cancer cells.

Objective To detect the expression of gonadotropin-releasing hormone (GnRH) in cervical cancer and to explore the correlation.Methods A total of 68 patients with cervical squamous cell carcinoma and 45 patients with chronic cervicitis were selected.Immunohistochemistry and hybridization in situ were used to detect the expression of GnRH in cancer tissue and cervicitis.Protein and nucleic acid level were observed in patients with GnRH.Results In 45 patients with cervicitis, both GnRH and GnRH mRNA expressions were negative in cervical epithelial tissue, while in 68 cases of cervical squamous cell cancer, the positive expression of GnRH in cancer tissues was 82.36%, and positive expression rate of GnRH mRNA was 64.71%.There were significant differences in levels of GnRH and GnRH mRNA between patients with cervicitis and cervical squamous cell carcinoma (P<0.05).The qualitative expression rates of GnRH in different clinical stages of cervical cancer were 55.56% of Ib, 87.10% of IIa and 100% of IIb, and qualitative expression rates of GnRH mRNA in different clinical stages of cervical cancer were 38.89% of Ib, 70.97% of IIa and 84.21% of IIb.There were significant differences in GnRH and GnRH mRNA levels between patients with different clinical stages of cervical cancer (P<0.05).Conclusion There is a high level of gonadotropin-releasing hormone in cervical cancer, which may be involved in the development of cancer cells.

Objective:To compare the results of invasive prenatal diagnosis and preimplantation genetic testing (PGT) and explore the underlying mechanism.Methods:Clinical data of pregnant women undergoing PGT and invasive prenatal diagnosis at the Sixth Affiliated Hospital of Sun Yat-sen University from January 2019 to December 2022 were collected. The results of PGT and invasive prenatal diagnosis were compared, and the outcomes of pregnancies were followed up. This study has been approved by the Medical Ethics Committee of the the Sixth Affiliated Hospital of Sun Yat-sen University (No. 2022SLYEC-491).Results:A total of 172 couples were included in this study, and 26 non-targeted variants were discovered upon prenatal diagnosis, including 10 cases (38.5%) by chromosomal karyotyping, 15 (57.7%) by chromosomal microarray analysis (CMA), and 1 (3.8%) by whole exome sequencing. The 10 karyotypic anomalies had included 6 chromosomal polymorphisms, 2 chromosomal mosaicisms, 1 paternally derived translocation, and 1 missed maternal chromosomal inversion. CMA has identified 15 copy number variations (CNVs), which included 11 microdeletions and microduplications, 3 loss of heterozygosity, and 1 low-level mosaicism of paternal uniparental disomy. One CNV was classified as pathogenic, and another one was likely pathogenic, whilst the remaining 13 were classified as variants of uncertain significance. Therefore, 8.7% of CNVs was detected by invasive prenatal diagnosis after PGT. 92.3% (24/26) of the non-targeted variants have been due to technological limitations of next-generation sequencing (NGS).Conclusion:Invasive prenatal diagnosis after PGT can detect non-targeted variants, which may further reduce the incidence of birth defects.

In December 2019, novel coronavirus pneumonia epidemic occurred in Wuhan, Hubei Province, and spread rapidly across the country. In the early stages of the epidemic, China adopted the containment strategy and implemented a series of core measures around this strategic point, including social mobilization, strengthening case isolation and close contacts tracking management, blocking epidemic areas and traffic control to reduce personnel movements and increase social distance, environmental measures and personal protection, with a view to controlling the epidemic as soon as possible in limited areas such as Wuhan. This article summarizes the background, key points and core measures in the country and provinces. It sent prospects for future prevention and control strategies.