1.Determination of magnoflorine in Coptidis Rhizoma and Phellodendri Chinensis Cortex by LC-MS.
Shengjun FAN ; Dahai YU ; Yuanqinl GU ; Mingyu ZHANG ; Lin ZHANG ; Guoyu LI
China Journal of Chinese Materia Medica 2010;35(24):3322-3324
A rapid and specific high performance liquid chromatography-mass spectrometric method was developed for determination of magnoflorine in Rhizoma Coptidis and Cortex Phellodendri Chinensis. Samples were extracted by methanol. Agilent Eclipse XDB-C18 ODS column (4.6 mm x 150 mm, 5 microm) was used, and mobile phase was methanol-water (60:40) at a flow rate of 0.8 mL x min(-1). Electrospray ionization model (ESI), and MRM model were used for quantification. The linear range of magnoflorine was 4.352-2720 microg x L(-1). The average recovery was above 98%. The method is simple, sensitive and accurate, it can be used for determination of magnoflorine in Rhizoma Coptidis and Cortex Phellodendri Chinensis.
Aporphines
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analysis
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Chromatography, High Pressure Liquid
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methods
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Drugs, Chinese Herbal
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chemistry
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Linear Models
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Mass Spectrometry
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methods
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Time Factors