Objective To improve the quality of life of the female patient after radical cystectomy. Methods Modified radical cystectomy and detenia cecocolon neobladder were performed in 4 female patients with bladder cancer.Three patients underwent radical cystectomy with preservation of the anterior wall of vagina.One patient underwent radical cystectomy with resection of the anterior wall of vagina. Results The patients were followed-up for 6 to 30 months.All 4 women achieved acceptable daytime continence,and 3 out of 4 achieved nocturnal continence.The vesical capacity ranged from 305 to 438 ml and intravesical pressure at maximum capacity ranged from 18 to 45 cmH 2O(1 cmH 2O=0.098 kPa);only 1 had nocturnal incontinence.The urinary dynamic examination showed the maximum urinary flow rate varied from 15.1 to 22.6 ml/s and residual urine volume ranged from 0 to 30 ml.The cystography showed there was no ureteral stenosis or reflux in the 4 patients.Their renal function and metabolism of electrolytes were normal. Conclusions Detenia cecocolon neobladder after modified radical cystectomy in selected women can obtain satisfactory continence and voiding results.

Objective:To investigate esomeprazole combined with early enteral nutrition on the immune function and intestinal flo-ra in the elderly by patients with severe acute pancreatitis. Methods:Totally 78 elderly by patients with severe acute pancreatitis were selected and randomly divided into the observation group (39 cases) and the control group (39 cases). The control group was treated with early enteral nutrition therapy, and the observation group was treated with esomeprazole additionally. The treatment course was 14 days. Results:The total effective rate of the observation group was higher that of the control group (P<0. 05). The abdominal pain relief time, recovery time of bowel sounds, abdominal distension relief time, and the time of serum amylase returning to normal and u-rine amylase returning to normal in the observation group were all better than those in the control group (P<0. 05). The incidence of intestinal flora disorders in the observation group was lower than that in the control group (P<0. 05). After the treatment, CD3+, CD4+ and CD4+/CD8+ increased in both groups (P<0. 05), and those in the observation group were higher than those in the con-trol group (P<0. 05). Serum amylase and urine amylase decreased in both groups after the treatment (P<0. 05), and the decrease in the observation group was more significant than that in the control group (P<0. 05). No serious adverse drug reactions appeared in both groups. Conclusion:Esomeprazole combined with early enteral nutrition in the elderly by patients with severe acute pancreatitis shows significant effect, and the effect may be related with improving the immune function and intestinal flora.

Objective To study the clinical efficacy of robotic sacral hysteropexy in treatment of uterine prolapse.Methods From January 2012 to December 2013,3 patients undergoing robotic sacral hysteropexy in treatment of uterine prolapse in General Hospital of People's Liberation Army were studied retrospectively.Operation time,blood loss and postoperative recovery exhaust time and pelvic organ prolapse quantification (POP-Q) staging were evaluated.Results Three patients were treated by robotic sacral hysteropexy successfully.The mean operation time was 221 minutes (210-240 minutes),mean blood loss was 45 ml.One case with Ⅱ degree perineal laceration patients simultaneously perineal repair,neither intranor post-operative complications occurred.The mean postoperative recovery exhaust time was 16 hours.At three months of follow-up,all 3 patients got satisfaction.Although one patient at the first six months of postoperation had leakage of urine when coughing,instruct exercise pelvic floor muscle function and acupuncture one month their symptoms disappear.Conclusion Robotic sacral hysteropexy pave the way for an effective option in the management of uterine prolapse.

Objective To compare the clinical effects of mini phalangeal external fixation and mini plate in the treatment of intraarticular fractures of the metacarpal bone head,and to provide a theoretical basis for clinical selection of surgical plan. Methods Seventy?four cases of metacarpal bone head intraarticular fractures treated in Huizhou People′s Central Hospital from March 2014 to March 2016 were selected as the research object. All cases were closed fractures and were randomly divided into the external fixation group and the mini plate group,37 cases in each group and treated respectively with the mini phalangeal external fixator and mini AO plate. The patients were followed up for 6 months to 1 year,the function of the metacarpophalangeal joint,the union of the fracture and the incidence of side effects were compared between the two groups. Results The excellent rates of the external fixation group at 6 months and I year after operation were 91. 9% ( 34/37) and 97. 3%( 36/37) respectively,which were significantly higher than those of the mini plate group ( 73. 0%( 27/37) ,81. 2%( 30/37 ) ) , the difference was statistically significant (χ2 = 7. 120, 7. 062, P<0. 05 ) . The complication rate of the external fixation group was 2. 7%( 1/37) ,which was significantly lower than that of the mini plate group ( 24. 3% ( 9/37 ) ) , the difference was statistically significant ( P=0. 014 ) . The average fracture healing time in the external fixator group was (2. 6±0. 4) months,which was significantly lower than that in the mini plate group (3. 1±0. 7) months,the difference was statistically significant (t=2. 40,P<0. 001) . Conclusion In the treatment of metacarpal bone head fracture,the external fixation has small trauma,can do cross joint fixation,and maintain a good fixation strength,and promote the healing of fracture and the recovery of metacarpophalangeal joint function. It is worthy of clinical application.

Objective To study the significance of artery blood gas(ABG)of neonate cord blood on the diagnosis of asphyxia neonatorum.Methods Neonates were divided into two groups:asphyxia group and control groups.ABG were measured by AVL blood gas analysis instrument.Results pH and PO2 in the asphyxial group were lower and PCO2 was higher than those in control group(P

Objective To apply robotic surgery of early ovarian malignancy tumors clinically and evaluate its feasibility in management for early ovarian cancer. Methods Using the da Vinci robotic surgical system, seven patients with early ovarian malignancy tumors (stage Ⅰ) underwent robotic surgery from April 2012 to September 2013. The average age was 45.7 years. Robotic surgeries approaches contained salpingo-oophorectomy,para-aortic lymphadenectomy, pelvic lymphadenectomy, omentectomy and appendectomy. Perioperative and follow-up clinical data were recorded. Results All robotic surgeries were successfully completed without the conversion to laparotomy. The mean operative time was 225 minutes (100-330 minutes). The average estimated blood loss was 171 ml (20-600 ml). No patients received blood transfusions. No intraoperative and postoperative complications were observed. The average number of pelvic lymph node dissected were 18.3 (11-34). The average number of para-aortic lymph node dissected were 3.7 (3-4). The mean follow-up time was 26.0 months after surgery (20-36 months). Currently, all patients had no tumor recurrence and survived. Conclusion Robotic surgery is feasible as a novel alternative approach in the treatment of early ovarian malignancy tumors.

OBJECTIVETo study the effect of high power microwave (HPM) radiation on the testicular germ cell apoptosis.

METHODSOne hundred and twenty-five Spraque-Dawley rats were randomly divided into two groups, unexposed control group and experimental group(further divided into four subgroups: 10 mW/cm2 5 min, 10 mW/cm2 10 min, 20 mw/cm2 5 min, and 20 mW/cm2 10 min), and then the experimental group was radiated with S wave band of 10 mW/cm2, 20 mW/cm2 high power microwave for 5 or 10 min. Testicular samples were taken at 6 h, 24 h, 48 h, 72 h and 5 d after radiation and separately studied. At the end of the process, testicular germ cell apoptosis was detected by in situ terminal deoxynucleotityl transferase mediated dUTP nick end labeling (TUNEL).

RESULTSThe number of apoptotic cells of the 6 h, 24 h and 48 h experimental groups at 5 min after 10 and 20 mW/cm2 radiation was remarkably larger than that of the controls (P < 0.01), especially after 10 mW/cm2 radiation, the number of the 6 h group reached the peak (161.27 +/- 5.90) /5 convoluted seminiferous tubules. The changes in the other experimental groups had no significant difference compared with the controls (P > 0.05).

CONCLUSIONHPM can increase germ cell apoptosis of the rat testis, which is related to the time of radiation and sample acquisition. In the condition of the present test, 5 minutes of HPM radiation may significantly enhance testicular germ cell apoptosis and damage, which in turn may influence the reproductive function of the rats.

Animals ; Apoptosis ; radiation effects ; Male ; Microwaves ; Rats ; Rats, Sprague-Dawley ; Spermatozoa ; pathology ; radiation effects ; Testis ; radiation effects

OBJECTIVETo evaluate the effect of ultrasound-guided focused ultrasound ablation (US-FUA) in the treatment of intramural, submucosal and subserosal uterine fibroids.

METHODSWe prospectively assessed the efficacy of US-FUA for treatment of symptomatic uterine fibroids by determining the volume ablation ratio immediately after the treatment and evaluating the volume changes of the uterine fibroids on contrast-enhanced MRI at 6 and 12 months after the treatment. The symptom severity score (SSS) was examined before and at 6 and 12 months after the treatment.

RESULTSA total of 110 women with 145 symptomatic fibroids undergoing US-FUA between August 2011 and December 2012 were enrolled. The fibroids were located intramurally in 72 (49.7%), submucosally in 49 (33.8%) and subserosally in 24 (16.6%) patients, who had comparable mean volume ablation ratios [(93.± 13.1)% vs (94.9 ± 9.3)% vs (89.6 ± 19.3)%, P>0.05]. The volume of the treated fibroids was reduced by (47.3 ± 20.8)%, (70.3 ± 22.2)% and (47.8 ± 13.6)% at 6 months after US-FUA and by (58.9 ± 19.9)%, (82.0 ± 21.2)%, and (50.5 ± 17.8)% at 12 months, respectively, demonstrating a more obvious volume reduction for submucosal fibroids (P<0.05). The SSS scores of the 3 groups before treatment were 29.1 ± 15.2, 34.8 ± 14.5 and 26.6 ± 23.4, respectively, which decreased significantly to 18.4 ± 15.8, 15.5 ± 10.7, and 11.6 ± 11.7 at 6 months (P<0.05) and to 18.9 ± 13.2, 16.8 ± 14.0, 11.5 ± 9.1 at 12 months (P<0.05). No major complications occurred in these patients during the 12-month follow-up.

CONCLUSIONUS-FUA can yield high ablation ratios for intramural, submucosal and subserosal fibroids and result in significant volume reduction and symptomatic improvement at 6 and 12 months after the treatment, suggesting its value as a new noninvasive option for fibroid treatment in patients who wish to preserve the uterus.

Female ; Follow-Up Studies ; High-Intensity Focused Ultrasound Ablation ; Humans ; Leiomyoma ; therapy ; Magnetic Resonance Imaging ; Treatment Outcome ; Uterine Neoplasms ; therapy

Objective: To explore the effects of miR-124-ROCK1 signal pathway in the damages of glomerular endothelial cells (GEnCs) induced by high glucose. Methods: Rat glomerular endothelial cells were cultured in different glucose concentrations: normal control group (NG: 5.5 mmol/L), high glucose group (HG: 30.0 mmol/L), and cells were treated with ROCK1 inhibitor Y27632, miR-124-3p mimic, miR-124-3p inhibitor. The expressions of ROCK1 activity, cell apotosis and tight junction proteins were detected by Western blot. The cell tight junction protein ZO-1 in those groups were assessed by laser scanning confocal microscope. Results: High glucose significantly decreased miR-124 expression (P<0.01), ROCK1 activity (P-MYPT1/MYPT1), and cell apoptosis (Cleaved-Caspase3/pro-Caspase3) were found increased while the tight junction proteins ZO-1and Occludin were found decreased in these cells (P<0.05 all P<0.01), However, when pretreated cells with ROCK1 inhibitor Y27632, these injuries were significantly reversed. In cells transfected with miR-124-3p mimic, p-MYPT1/MYPT1 was decreased. p-MYPT1/MYPT1 was however increased in cells transfected with miR-124-3p inhibitor (P<0.05), indicating that miR-124 could directly inhibit ROCK1 activity. The increased ROCK1 activity and apoptosis, as well as the decreased tight junction proteins induced by high glucose were significantly suppressed as miR-124-3p mimic transfected in GEnCs. Conclusions According to our experiments, high glucose suppressed miR-124 in glomerular endothelial cells, consequenctly activating ROCK1 activity to damage endothelial cells. MiR-124 overexpression could ameliorate these damages induced by high glucose, suggesting that miR-124 might be a new therapeutic target to prevent glomerular endothelial cells injuries in diabetic nephropathy.