Objective To study the clinical efficacy of robotic sacral hysteropexy in treatment of uterine prolapse.Methods From January 2012 to December 2013,3 patients undergoing robotic sacral hysteropexy in treatment of uterine prolapse in General Hospital of People's Liberation Army were studied retrospectively.Operation time,blood loss and postoperative recovery exhaust time and pelvic organ prolapse quantification (POP-Q) staging were evaluated.Results Three patients were treated by robotic sacral hysteropexy successfully.The mean operation time was 221 minutes (210-240 minutes),mean blood loss was 45 ml.One case with Ⅱ degree perineal laceration patients simultaneously perineal repair,neither intranor post-operative complications occurred.The mean postoperative recovery exhaust time was 16 hours.At three months of follow-up,all 3 patients got satisfaction.Although one patient at the first six months of postoperation had leakage of urine when coughing,instruct exercise pelvic floor muscle function and acupuncture one month their symptoms disappear.Conclusion Robotic sacral hysteropexy pave the way for an effective option in the management of uterine prolapse.

Objective To investigate the treatment effects and mechanisms of pyrroloquinoline quinine(PQQ) on defective teeth and mandible in Bmi-1 knockout mice.Methods Male and female Bmil+/-mice were paired with each other from the same nest.At the age of 7 weeks,the mice were divided into three groups,the wild type mice received normal diet(10 mice,WT group),Bmil-/-mice received normal diet (10 mice,BKO group),and the Bmi1+/-mice received normal diet and PQQ diet(10 mice,BKO+PQQ group).X-ray and micro-CT were used to detect mandible and dental size and bone mineral density.HE staining,histochemical and immunohistochemical methods were respectively used to detect alveolar bone thickness of cortical bone,predentin thickness of mandibular first molar,mandibular osteoblast number and osteoclast number.Flow cytometry was used to detect reactive oxygen species(ROS) levels of various organs(femur,thymus and liver).The data were statistically analyzed with one-way ANOVA and t test.Results Compared with BKO mice,BKO+PQQ mice partially rescued total body phenotype,increased body weight and prolonged survival time.X-ray and micro-CT showed the size of the mandible and teeth and bone mineral density of PQQ+BKO mice increased compared with BKO mice.In PQQ+BKO mice,mandibular alveolar bone cortical thickness[(68.65±0.25) μm] was significantly different from that in BKO mice[(42.45±0.35) μm] (P＜0.01).There was significant difference in predentin thickness of mandibular first molar between PQQ + BKO mice[(4.25 ± 0.15) μm] and BKO mice[(31.55 ± 0.35) μm] (P＜0.001).The number of osteoblasts in the mandible of BKO+PQQ mice[(38.45±0.25) cell/mm3] was significantly higher than that in the BKO mice[(18.15±0.55) cell/mm3] (P＜0.01).However,the number of osteoclasts in the BKO+PQQ mice [(9.45±0.25) cell/mm3] was significantly lower than that in the BKO group[(14.25±0.35) cell/mm3] (P＜0.01).Compared with the BKO group,ROS levels of the femur,thymus and liver in the BKO + PQQ mice were significantly decreased(P＜0.01).Conclusions The results indicate that PQQ may have treatment effects on defective teeth and mandible through promoting osteoblast bone formation and reducing osteoclast bone resorption,scavenging ROS and reducing DNA damage.

Nuclear medicine plays an indispensable role in the diagnosis,treatment,and prognosis of a wide range of diseases.Nuclear medicine using radionuclides for diagnosis has the advantages of accuracy,speed,high sensitivity and high resolution.Currently,several radionuclides play pivotal roles in disease diagnosis.This article primarily examines the clinical application and research of diagnostic radionuclides,including 18 F,89 Zr,68 Ga,99m Tc,131 I,123 I,and 11 C.The objective is to offer valuable insights for disease diagnosis and staging of diseases.