Objective:To approach the role of lymphangiogenesis in oral squamous cell carcinoma(OSCC). Methods: Immunohistochemical double staining technique was utilized to distinguish lymphatic vessels from blood vessels. MLVD was determined by manual counting to analyze the relationship with lymph node metastasis. Results: MLVD was significantly higher in 40 OSCC than 14 normal mucosa(P<0.01). MLVD in OSCC showed a statistically significant relationship with regional lymph node metastasis, depth of invasion(P<0.01). Conclusion: Lymphangiogenesis might occur in growth, infiltration and metastasis of OSCC. Detection of MLVD in OSCC might be important to evaluate tumor metastasis and judge prognosis.

As a new surface technology, microarc oxidation can be applied to the ceramic coating with bioactivity on Ti alloy. With good biocompatibility, high bond strength and short healing period, the film is worth applying to clinical operation. In this paper such information of microarc oxidation is reviewed as its developing history, research progress and basic principle. The structure, properties and bioactivity of the ceramic coating are also introduced.

Objective To study the significance of artery blood gas(ABG)of neonate cord blood on the diagnosis of asphyxia neonatorum.Methods Neonates were divided into two groups:asphyxia group and control groups.ABG were measured by AVL blood gas analysis instrument.Results pH and PO2 in the asphyxial group were lower and PCO2 was higher than those in control group(P

Objective To compare the clinical effects of mini phalangeal external fixation and mini plate in the treatment of intraarticular fractures of the metacarpal bone head,and to provide a theoretical basis for clinical selection of surgical plan. Methods Seventy?four cases of metacarpal bone head intraarticular fractures treated in Huizhou People′s Central Hospital from March 2014 to March 2016 were selected as the research object. All cases were closed fractures and were randomly divided into the external fixation group and the mini plate group,37 cases in each group and treated respectively with the mini phalangeal external fixator and mini AO plate. The patients were followed up for 6 months to 1 year,the function of the metacarpophalangeal joint,the union of the fracture and the incidence of side effects were compared between the two groups. Results The excellent rates of the external fixation group at 6 months and I year after operation were 91. 9% ( 34/37) and 97. 3%( 36/37) respectively,which were significantly higher than those of the mini plate group ( 73. 0%( 27/37) ,81. 2%( 30/37 ) ) , the difference was statistically significant (χ2 = 7. 120, 7. 062, P<0. 05 ) . The complication rate of the external fixation group was 2. 7%( 1/37) ,which was significantly lower than that of the mini plate group ( 24. 3% ( 9/37 ) ) , the difference was statistically significant ( P=0. 014 ) . The average fracture healing time in the external fixator group was (2. 6±0. 4) months,which was significantly lower than that in the mini plate group (3. 1±0. 7) months,the difference was statistically significant (t=2. 40,P<0. 001) . Conclusion In the treatment of metacarpal bone head fracture,the external fixation has small trauma,can do cross joint fixation,and maintain a good fixation strength,and promote the healing of fracture and the recovery of metacarpophalangeal joint function. It is worthy of clinical application.

[Objective] To investigate the therapeutic effect of Radix Astragali on neonatal hypoxic-ischemic encephalopathy (NHIE) and to explore its immunoregulation.[Methods] Fifty cases of NHIE were randomized to groups A and B. Both groups were given routine treatment and group A was treated with Radix Astragali injection additionally. Symptoms and signs, scoring of neonatal behavior neurological assay (NBNA) and subtypes of peripheral blood T lymphocyte were observed before and after treatment, [Results] Time of symptom-sign disappearance was 1.4 d shorter in group A than that in group B (t = 2.06, P

Objective To measure the morphological parameters of the acromion with CT and to analyze their match with the hook plate. Methods From October 2009 to February 2010,spiral CT scanning (with Somatom Emotion16) and three-dimensional reconstruction of bilateral shoulders were conducted in 61 Chinese subjects.They were 24 men and 37 women,aged from 20 to 83 years (average,45.2 years).The thickness,length and width of the acromion were measured and the subacromial shape was observed with software of the CT system to analyze the match between the hook plate and the acromion.Results The mean thickness of the acromion was 0.85 ± 0.13 cm in all subjects,0.94 ± 0.12 cm in males and 0.79 ± 0.10 cm in fe males,with a significant difference between males and females ( t =2.382,P =0.202).The mean acromion length was 2.08 ± 0.20 cm in all subjects,2.09 ± 0.21 cm in males and 2.06 ± 0.18 cm in females,with no significant difference between males and females( t =1.541,P =0.129).The mean acromion width was 3.81 ± 0.52 cm in all subjects,4.34 ± 0.32 cm in males and 3.47 ± 0.25 cm in females,with a significant difference between males and females ( t =2.296, P =0.025 ). Conclusions The acromial morphology varies significantly between genders in Chinese population,particularly in the thickness and width.It is,therefore,necessary to modify the morphological parameters of the hook plate to match better the gender difference in Chinese population.It is optimal that the hook plate should allow individualized pre-moulding to enhance its therapeutic efficacy.

Objective To explore the risk of pituitary dysfunction for spontaneous subarachnoid hemor -rhage( s-SAH) patients and to evaluate its correlation with clinical data .Methods 63 cases of patients with s-SAH were selected.Indicators(serum adrenocorti cotrophic hormone (ACTH), growth hormone(GH), insulin-like growth factor-1(IGF-1), thyroid stimulating hormone(TSH), thyroxine(T4), triiodothyronine(T3), folli-cle-stimulating hormone(FSH), luteinizing hormone(LH), total testosterone(in males), estradiol(in females) and prolactin)dynamic observation were tested 7 days, 1 year, 2 years, 3 years after the onset.Results Thirty patients( 47.6%) had some type of pituitary dysfunction .ACTH deficiency was the most frequent disorder (14.3%), followed by GH deficiency(12.7%), hypogonadatrophic(6.3%), hypothyroidism(3.2%)and high prolactin(1.6%).six patients(9.5%)showed deficiencies in more than one axis .Hormone deficiency was relat-ed to vasospasm, Fisher grade 4(P<0.05)and recovery time.Conclusions A greater incidence of hormone de-ficiency after s-SAH was related with vasospasm , Fisher grade and recovery time .Follow-up time should be ex-tended.

OBJECTIVETo study the clinical therapeutic effect of anisodamine on respiratory function after severe brain injury.

METHODSNinety patients with respiratory dysfunction following severe brain injury were divided into two groups: a treatment group (n = 45, treated with routine therapy plus anisodamine) and a control group (n = 45, treated with routine therapy only). The pulmonary ventilation function and oxygenation function were compared between the two groups.

RESULTSIn the treatment group, 12 hours after treatment the respiratory rate reduced, the partial pressure of carbon dioxide (PCO(2)), the partial pressure of oxygen in arterial blood (PaO(2)) and oxygenation exponent increased, the dead space ventilation dose and the pulmonary alveolus-partial pressure of arterial oxygen difference decreased, and the ventilation function of the respiratory tract and pulmonary oxygenation function improved. There was a significant difference between the two groups (P < 0.01). No side-effect was found except a slight increase of intracranial pressure and heart rate.

CONCLUSIONSAnisodamine can improve pulmonary ventilation function and oxygenation function and decrease the incidence of hypoxemia markedly. It is effective in treating respiratory dysfunction after severe brain injury.

Adolescent ; Adult ; Aged ; Brain Injuries ; complications ; diagnosis ; mortality ; Child ; Dose-Response Relationship, Drug ; Drug Administration Schedule ; Female ; Follow-Up Studies ; Humans ; Infusions, Intravenous ; Injury Severity Score ; Male ; Middle Aged ; Primary Prevention ; methods ; Probability ; Pulmonary Gas Exchange ; Reference Values ; Respiratory Function Tests ; Respiratory Insufficiency ; etiology ; mortality ; prevention & control ; Solanaceous Alkaloids ; administration & dosage ; Survival Rate ; Treatment Outcome

Objective:To investigate whether the prophylactic use of a dose of sensitive antibiotics before revision for periprosthetic joint infection (PJI) may affect the positive rate of intraoperative specimen culture.Methods:This prospective study recruited the patients who underwent revision due to PJI from July 1, 2017 to February 1, 2019 at Department of Orthopaedics, The First Affiliated Hospital to Fujian Medical University. After use of antibiotics was stopped in all patients for 2 weeks before operation, synovial fluid was extracted for culture to confirm pathogenic bacteria and drug sensitivity and some/all of the prostheses were removed during operation. According to their sequence number of admission, the patients were randomly divided into group A and group B. Samples were taken in group A after a dose of sensitive antibiotics was administered 30 to 60 minutes before revision while a dose of sensitive antibiotics was given in group B after all samples were taken. Intra-operatively, synovial fluid, tissue grinding fluid (TGF) and ultrasonic prosthesis lysate (UPL) were taken for aerobic and anaerobic culture. According to whether there was a positive culture of at least one microbiological specimen, the preoperative and intraoperative culture results were analyzed and compared between the 2 groups.Results:A total of 32 PJI patients were included in this study due to positive culture of synovial fluid before operation, with 16 cases in group A and 16 in group B. The most common infection bacteria were staphylococci (59.3%, 19/32). There was no significant difference in age, gender, mode of operation, Tsukayama classification, prosthesis removal, preoperative ESR, CRP, synovial fluid white blood cell count (SF-WBC) or polymorphonuclear cell percentage (PMN) between the 2 groups. The positive rates of synovial fluid, tissue, TGF and UPL were 81.3% (13/16), 62.5% (10/16), 93.8% (15/16) and 93.8% (15/16) for group A, and 87.5% (14/16), 68.8% (11/16), 93.8% (15/16) and 100.0% (16/16) for group B, showing insignificant differences between the 2 groups ( P>0.05). The positive rates of TGF and UPL culture showed no significant difference between them in group A or in group B ( P>0.05), but they were significantly higher than those of traditional tissue culture ( P<0.05). Conclusions:As prophylactic use of antibiotics before PJI revision may not affect the positive rate of intraoperative specimen culture, it is not necessary to postpone use of prophylactic antibiotics before PJI revision. Furthermore, as positive rates of TGF and UPL culture are similar but significantly higher than those of traditional tissue culture, tissue grinding can be used to improve the positive rate of tissue culture.

Objective:To investigate the effect of pyrroloquinoline quinone (PQQ) on age-related skin aging in mice and its mechanisms.Methods:Thirty Kunming mice were fed in specific pathogen-free condition, and equally divided into 3 groups: young group was fed with a normal diet for 8 months, old group was fed with a normal diet for 20 months to establish a mouse model of natural aging, and PQQ group was fed with PQQ-containing forages (4 milligrams of PQQ per kilogram of normal forages) for 20 months. After feeding, the mouse dorsal skin tissues were obtained, hematoxylin and eosin (HE) staining was performed to measure the epidermal and dermal thickness, Masson staining to detect changes in total skin collagen, immunohistochemical study to detect changes in expression of the proliferation marker Ki67, transmission electron microscopy to detect changes in autophagosomes in the mouse skin, and Western blot analysis to determine the expression of autophagy-related proteins Beclin1, LC3Ⅱ/LC3Ⅰ, and p62. Statistical analysis was carried out by using one-way analysis of variance for intergroup comparisons followed by least significant difference (LSD) - t test for multiple comparisons. Results:HE and Masson staining showed that the epidermal and dermal thickness and the percentage of area of dermis stained positive for collagen among the total area of dermis in the tested region were significantly lower in the old group (15.67 ± 0.36 μm, 87.95 ± 11.86 μm, 22.12% ± 1.72%, respectively) than in the young group (29.37 ± 0.25 μm, 264.93 ± 10.34 μm, 45.03% ± 1.54%, respectively, all P<0.05) , and significantly higher in the PQQ group (25.53 ±0.47 μm, 145.01 ± 9.71 μm, 31.17% ± 1.20%, respectively) than in the old group (all P<0.05) . Immunohistochemical study revealed that the expression of Ki67 was significantly lower in the old group (13.74% ± 3.06%) than in the young group (29.07% ± 2.79%, P<0.05) and PQQ group (21.20% ± 1.47%, P<0.05) . Transmission electron microscopy showed that the number of autophagosomes in the skin was significantly higher in the old group than in the young group ( P<0.05) , but significantly lower in the PQQ group than in the old group ( P<0.05) . As Western blot analysis revealed, the old group showed significantly decreased Beclin1 expression and LC3 Ⅱ/LC3 Ⅰ ratio, but significantly increased p62 expression compared with the young group (all P<0.05) ; compared with the old group, the PQQ group showed significantly increased Beclin1 expression and LC3Ⅱ/LC3Ⅰratio, but significantly decreased p62 expression (all P<0.05) . Conclusion:PQQ can delay the age-related skin aging in mice, likely by increasing the proliferative capacity of mouse skin cells and promoting skin autophagy.