To observe its proliferation and cytolytic activity in vitro induced by autologous soluble tumor antigen and IL-2, PBMC costimulated with TSA extracted from gastric tumor by biochemical procedure and IL-2 was cultured. The proliferation and cytotoxicity were evaluated by MTT method and the phenotype was analysed by APAAP method. TSA alone did not induce PBMC proliferation while TSA (10?g/ml) with IL-2 presented syngenic stimulating effect which was time and concentration dependent. The cytolytic unit of PBMC induced with IL-2/TSA was 86.3% vs 48.6% with IL-2 alone. The phenotypic measurement demonstrated that TSA enhanced CD25 and CD8 expression significantly. The observation offered experimental data for further research work about tumor activated killer(TAK) cells. The results indicated that TAK cells would be a new type of immunoactive cells in adoptive immunotherapy against tumor.

Objective To develop an assessment indicator system of function for standardized patients in the objective structured clinical examination for nursing specialty,which provides the basis for the standardized training and assessment of standardized patients.Methods The Delphi method was used to determine this indicator system.Results After two rounds of the Delphi procedure,the assessment indicator system was drawn-up,which included 3 first-class indicators and 12 second-class indicators and 35 third-class indicators with weights.Conclusions The achievements of this study may provide reference for nursing educators to screen standardized patients strictly and improve the quality of standardized patients continuously,and also provide the basis for development of scale or instrument in the further.

Objective To investigate the association between the inflammatory factors and carotid atherosclerosis in patients with cerebral infarction. Methods The carotid intima-media thickness(IMT), plaque and inflammatory indexes were correlative analysed in 244 patients with acute cerebral infarction. Results Inflammatory factors ( monocyte proportion, levels of fibrinogen and CRP,erythrosedimentation) were associated with carotid atherosclerosis. Monocyte proportion (ORIMT =1.231,P

Objective To explore the vascular endothelial function in individuals with normal glucose tolerance (NGT),impaired glucose tolerance (IGT),and type 2 diabetes mellitus,and to study the role played by PPARγcoactivator 1 α (PGC-1 α) in diabetic macrovascular function.Methods A total of 94 subjects with NGT,87with IGT,and 306 with type 2 diabetes mellitus were recruited in the study.Carotid flow-mediated vasodilatation (FMD),glyceryl trinitrate dilatation (GTN),and carotid intima-media thickness (CIMT) were assessed by highresolution B-mode color vascular Doppler instrument.Finally,correlation analysis was conducted.NO,Akt-Ser473phosphorylation levels,IL-β,and PGC-1 α mRNA expression levels were measured by means of nitrate reductase,western blot,and reverse transcriptase real-time fluorescence quantitative PCR assay methods in peripheral blood mononuclear cells respectively.Results FMD in newly-diagnosed type 2 diabetic group was significantly lower than that in NGT and IGT groups(P＜0.01).GTN and CIMT showed no difference.PGC-1 α mRNA transcription level in diabetic group was significantly lower than that in non-diabetic groups (P＜0.01).Correlation analysis showed that PGC-1 α was positively correlated with FMD and GTN (P＜0.05 or P＜0.01).Logistic regression analysis revealed that PGC-1 α was independently associated with FMD (P ＜ 0.01) after adjusting other influencing factors.Conclusion FMD is a more sensitive early warning indicator of endothelial function than CIMT and GTN.As indices of atherosclerosis,CIMT and GTN are more or less focused on the progression of macrovascular complications.PGC-1 α is a protective factor of macro-vasculature in diabetes mellitus,and its effect may be achieved through the protection of the vascular endothelium.

Objective:To investigate the relationship between the expression of serum exsomal miRNA-155-5p (miR-155-5p) and prognosis in patients with esophageal squamous cell carcinoma (ESCC).Methods:A total of 336 samples from ESCC patients in Fujian Provincial Cancer Hospital from October 2014 to December 2015 were collected. The relative expression levels of serum exsomal miR-155-5p were detected by using real-time fluorescent quantitative polymerase chain reaction (qRT-PCR). Cut-off value of the expression levels of serum exsomal miR-155-5p was determined by using X-tile software. Based on the optimal cut-off value, patients were divided into miR-155-5p low expression group and miR-155-5p high expression group. The survival curve was drawn by using Kaplan-Meier method and Cox proportional hazards regression model was used to make survival analysis.Results:The cut-off value of serum exsomal miR-155-5p expression level was 2.340. According to the cut-off value, patients were divided into miR-155-5p low expression group (<2.340) of 51 cases and miR-155-5p high expression group (≥2.340) of 285 cases. There were no statistical differences in age ( χ2 = 0.020, P = 0.887), gender ( χ2 = 0.283, P = 0.595), tumor location ( χ2 = 0.063, P = 0.977), differentiation grade ( P = 0.474), clinical staging ( χ2 = 3.996, P = 0.136) and surgery treatment ( χ2 = 0.941, P = 0.332) of patients in both groups. ESCC patients in serum exsomal miR-155-5p high expression had a higher risk of death compared with patients in miR-155-5p low expression group ( HR = 1.763, 95% CI 1.049-2.961, P = 0.032). Conclusion:The high expression level of serum exsomal miR-155-5p is associated with poor prognosis in ESCC patients and it could be used as a prognostic new marker in ESCC patients.

ObjectiveTo establish a hospice care training system for nurses in general hospitals and evaluate its implementation effects, and to explore a new path for hospice care education. MethodsThe nurses from 7 clinical departments in the First Affiliated Hospital of He＇nan University of Chinese Medicine from September 2017 to June 2018 were selected by convenience sampling with 24 nurses from each department and randomly divided into the general training group (12 for every department) and in-depth training group (12 for every department). Based on the classic education theory, the nurse hospice care training system for general hospital was established from five aspects including goal, content, method, management and teachers in the in-depth training group and the general training group received the training according to hospice care nursing plan implementation rules of the First Affiliated Hospital of He＇nan University of Chinese Medicine. The two groups were compared in terms of the training effects. ResultsAfter training, in the in-depth training group, the scores of theoretical assessment and skill assessment of hospice care of nurses in the 7 departments were all higher than those in general training group, and the differences were statistically significant (P＜ 0.05). ConclusionsThe general hospital nurse hospice care training system can effectively improve the employees＇knowledge reserve and skills level of hospice care; the strengthening of the construction of hospice care training system is important to guarantee the qualified hospice care nursing team building and to improve the quality of hospice care services.

OBJECTIVE: To explore the relationship among loneliness at workplace, job burnout, and turnover intention in prison policemen. METHODS: A convenience sampling method was used to select a total of 290 prison policemen officers as the study subjects in Guangxi Zhuang Autonomous Region. The Workplace Loneliness Scale, Job Burnout Scale and Turnover Intention Scale were used to investigate the loneliness at workplace, job burnout and turnover intention respectively.RESULTS: The scores of turnover intention of 31-40 age group were higher than that of 41 or over age group(P<0.01). The scores of turnover intention in the length of service 1-5 years group and 6-10 year length of service group were higher than that of length of service over 16 years or more(P<0.05). The scores of turnover intention of section group policemen were higher than that of division group(P<0.05). The loneliness in the workplace was positively correlated with job burnout[Spearman rank correlation coefficient(r_S)=0.343, P<0.01], and turnover intention(r_S=0.392, P<0.01), and job burnout was positively correlated with turnover intention(r_S=0.408, P<0.01). Job burnout was the intermediate variable between loneliness in the workplace and turnover intention with a mediating effect rate of 60.2%. CONCLUSION: The job burnout of prison policemen plays a partial role in mediating the influence of loneliness at workplace on turnover intention.