Objective To investigate the effects of Yun-Pi Prescription on hemolysin antibody,and approach its mechanism of regulating immune function.Methods Forty-eight KM mice were randomly divided into high,medium and low dose of Yun-Pi Prescription groups,which were given 20,10,5 mL/kg Yun-Pi Prescription respectively,and one control group with no intervention.From the seventh day,all groups were injected with chicken red blood cell for seven days.Levels of serum hemolytic optical density were measured in all groups.Results Compared with control group,levels of serum hemolytic optical density in Yun-Pi Prescription groups increased significantly(P

Objective To observe the effect of Yun-Pi Prescription in different dose on small intestinal function of splenic asthenia rats, so as to explore mechanisms of Yun-pi Prescription in treatment of children apositia. Methods Experimental rats were randomly divided in to six groups:the control group, the model group, the positive control group (Xiao shi Jian er Syrup group), Yun-Pi Prescription in high, middle and low dose group. Measure the changes of body weight and food intake, observe excretory rate of D-xylose of rats. Results Yun-Pi Prescription could increase body weight and food intake, improve excretory rate of D-xylose in splenic asthenia rats. Conclusion Yun-Pi Prescription could improve the small intestinal absorptive function of splenic asthenia rats with dose-effect relationship.

[Objective] Study effect of Yun-Pi Prescription on regulating the immune function,approach the mechanism of Yun-Pi Prescription on how to enhance immune function.[Method]All the mice were divided into groups: the normal group,the model group,the positive control group treated with Xiao Shi Jian Er Syrup,and Yun-Pi Prescription treatment groups including high,middle and low dose;determine the index number of immune organ,phagocytic count and phagocytize activity of normal mice.[Results]The high and middle dose of Yun-Pi Prescription can obviously enhance the phagocytic count and phagocytize activity,Yun-Pi Prescription has an up-regulation effect on immune function.

[Objective] To observe the effects of Yun-Pi Prescription of different doss on endurance of rats with splenic asthenia and to explore the mechanism of Yun-pi Prescription in treatment of children apositia.[Methods] To set up the animal model of splenic asthenia with reserpine.Experimental rats were randomly divided into six groups:the control group,the model group,the positive control group.Xiao Shi Jian Er Syrup,and Yun-Pi Prescription treatment groups including high,middle and low dose.Changes of body weight,food intake and swimming time were measured to determine the endurance of rats.[Results] Yun-Pi Prescription could increase body weight and food intake,prolong the swimming time of splenic asthenia rats.[Conclusion] Yun-Pi Prescription could effectively counteract fatigue and improve endurance of rats with splenic asthenia and it is useful in treatment rats with splenic asthenia.

Objective To observe the changes of tear film stability,goblet cell and mucin 5AC expression in conjunctivochalasis patients,and explore the mechanism of conjunctivochalasis.Methods Conjunctivochalasis patients (30 cases) and single age-related cataract patients (15 cases) were collected as conjunctivochalasis group and normal control group.Eye symptom assessment (OSDI score),tear break-up time (BUT),Schirmer Ⅰ test,tear fern crystallization tests were performed for all the selected persons.Conjunctival rescent-shaped resections were made for all the conjunctivochalasis patients.Conjunctival tissue samples were stained by HE staining,AB staining,mucin 5AC immunohistochemical staining from the conjunctivochalasis group and norral control group respectively,and then statistical analysis was made.Results The OSDI score in the conjunctivochalasis group (37.80 ± 8.94) was significantly higher than that in the normal control group (11.40 ±4.08) (P ＜0.01).BUT in the conjunctivochalasis group (6.70 ± 2.76) s was significantly lower than that in the normal control group (13.67 ± 3.48) s (P ＜ 0.01).Schirmer Ⅰ test in the conjunctivochalasis group (6.23 ± 3.13) mum was significantly lower than the normal control group (13.40 ± 3.74)mm (P ＜ 0.01).Tear ferbing crystallization of the conjunctivochalasis group was decreased significantly compared with the normal control group (x2 =14.309,P =0.003).Light microscopic showed that conjunctival thickness was thinned,collagen fibers were less,elastic fiber was reduced,the lamina propria and interstitial were congestion and edema,the number of goblet cells was significantly reduced,and the positive staining of mucin 5AC staining was significantly lower in the conjunctivochalasis group than in normal control group (x2 =9.499,P =0.023).Conclusion For patients with conjunctivochalasis,the tear film function is affected,goblet cells are decreased,tear fern crystallization is decreased,mucin 5AC content is decreased,which finally leads the excessive conjunctival relaxation and abnormal ocular surface and tear.

Objective:To explore the dynamic variation of serum neuron‐specific enolase (NSE) and its clinical significance in patients with acute organophosphate poisoning .Methods:Ninety patients with acute organophosphate poisoning were enrolled into case group ,and other 90 healthy subjects were enrolled as control group .Venous blood samples of 3 .0 mL were collected from the all 180 subjects at admission .Apart from the 8 patients died on day 1 ,the other 172 subjects underwent collection of 3 .0 mL venous blood samples on day 2 ,day 3 ,day 5 ,day 7 .Serum NSE concentration was measured by enzyme‐linked immu‐nosorbent assay ,while cholinesterase(ChE) was determined by photoelectric colorimetry .Results:Eight patients in case group died on admission day ,of whom the average concentration of NSE and ChE were (131 .72 ± 10 .84) ng/mL ,(199 ± 228) U/L , respectively .Concentration of NSE of the rest 82 patients on day 1 ,day 2 ,day 3 were (54 .56 ± 5 .46) ng/mL ,(36 .18 ± 5 .27) ng/mL ,(20 .56 ± 4 .64) ng/mL ,respectively ,and concentration of the control group on the corresponding time points were (9 .72 ± 3 .92) ng/mL ,(9 .48 ± 3 .66 )ng/mL ,(9 .86 ± 3 .62)ng/mL ,respectively .Statistically significant differences were de‐tected between two groups(P< 0 .01) .Average concentration of ChE of the rest 82 patients on day 1 ,day 2 ,day 3 were (1628 .60 ± 112) U/L、(2113 .59 ± 324) U/L、(2474 .75 ± 711) U/L ,respectively ,and those in the control group on the corre‐sponding time points were (5982 ± 215) U/L ,(5651 ± 136) U/L ,(5783 ± 764)U/L ,respectively .Statistically significant differences were detected between two groups(P<0 .01) .The concentrations of NSE were in significantly negative correlation with that of ChE in mild ,middle and severe poisoning patients on day 1 ,day 2 ,day 3 ,and in positive correlation with the poi‐soning severity .The correlation coefficients rs>0 .05 ,P<0 .05 .Conclusions:Serum NSE may be used as an reference indica‐tor in the diagnosis and treatment of acute organophosphate poisoning .

Background and Objectives: p21, an important member of the Cip/Kip family, is involved in inhibitory effects of RUNX1b overexpression during the early stage of human hematopoiesis. Methods: and Results: We established a human embryonic stem cell (hESC) line with inducible expression of p21 (p21/hESCs). Overexpression of p21 did not influence either mesoderm induction or emergence of CD34＋ cells, but it significantly decreased the production of CD43＋ cells and changed the expression profile of hematopoiesis-related factors, leading to the negative effects of p21 on hematopoiesis. Conclusions In RUNX1b/hESC co-cultures when RUNX1b was induced from D0, perturbation of the cell cycle caused by upregulation of p21 probably prevented the appearance of CD43＋ cells, but not CD34＋ cells. The mechanisms via which CD34＋ cells are blocked by RUNX1b overexpression remain to be elucidated.

Objective To investigate the exposure to benzene,methylbenzene,and dimethylbenzene in workers in a petrochemical enterprise in Shanghai,China,and to conduct occupational hazard risk assessment.Methods The environmental monitoring data on benzene series in the workplace of this petrochemical enterprise from 2010 to 2014 were collected,as well as workers' general status.The inhalation risk assessment model developed by United States Environmental Protection Agency (EPA) was used for risk assessment to determine the occupational hazard risk level of benzene,methylbenzene,and dimethylbenzene.Results The pass rate of the monitoring concentration of benzene,methylbenzene,and dimethylbenzene in the workplace of this petrochemical enterprise was 100％.The results of the EPA model showed that benzene had a high carcinogenic risk,benzene and dimethylbenzene had a high non-carcinogenic risk,and methylbenzene had a low noncarcinogenic risk.Conclusion The workers exposed to benzene in this petrochemical enterprise have a high health risk,and the EPA model can be used for occupational hazard risk assessment in chemical industry.

Objective To investigate the exposure to benzene,methylbenzene,and dimethylbenzene in workers in a petrochemical enterprise in Shanghai,China,and to conduct occupational hazard risk assessment.Methods The environmental monitoring data on benzene series in the workplace of this petrochemical enterprise from 2010 to 2014 were collected,as well as workers' general status.The inhalation risk assessment model developed by United States Environmental Protection Agency (EPA) was used for risk assessment to determine the occupational hazard risk level of benzene,methylbenzene,and dimethylbenzene.Results The pass rate of the monitoring concentration of benzene,methylbenzene,and dimethylbenzene in the workplace of this petrochemical enterprise was 100％.The results of the EPA model showed that benzene had a high carcinogenic risk,benzene and dimethylbenzene had a high non-carcinogenic risk,and methylbenzene had a low noncarcinogenic risk.Conclusion The workers exposed to benzene in this petrochemical enterprise have a high health risk,and the EPA model can be used for occupational hazard risk assessment in chemical industry.

Objective: To discuss the effect of high-density fat-binding SVF-GEL in female facial lipofilling. Methods: This is a retrospective study including 32 female patients, received facial fat transplantation during June 2017 to June 2018 in Yichun College. Each patient underwent high-density fat-binding SVF-GEL transplantation for facial surgery. Patients′satisfaction with the surgery and the rate of secondary surgery was evaluated. Fat was harvested from the inner thigh, centrifuged at 1200 g for 3 min, and the liquid was removed. The upper 2/3 part is prepared for SVF-GEL, for further used in delicate lipofilling in eyelid, tear groove and nasolabial groove. The lower 1/3 high density fat was used for volume restoration, such as forehead, temporal area and cheek. Results: All patients had significant improvements in facial contours with mild swelling and short recovery time. The satisfaction rate was 68.8%(22/32), and the second operation rate was 15.6%(5/32). Conclusions High-density fat-binding SVF-GEL transplantation can achieve good results in correcting facial volume loss.