Objective To investigate the expression of excision repair cross-complementation group 1 (ERCC1) and ribonucleotide reductase subunit M1 (RRM1) in patients with esophageal squamous cell carcinoma,and the relationship between the expression of ERCC1 and RRM1 genes and chemotherapy sensitivity.Methods A total of 77 patients with esophageal squamous cell carcinoma admitted in hospital from Jan.2008 to Dec.2012 were recruited.ERCC1 and RRM1 mRNA levels in the cancerous tissue were detected by real-time fluorescent quantitative RT PCR.The relationship between short-term effects of chemotherapy and ERCC1 and RRM1 mRNA levels was analyzed.Results Levels of mRNA in stages Ⅰ a,Ⅰ b,Ⅱ a,and Ⅱ b were (0.578±0.081),(0.560±0.084),(0.521±0.080),(0.464±0.091) for ERCC1 and(0.511±0.089),(0.539± 0.086),(0.584±0.092),(0.637±0.101)for RRM1,respectively.As the clinical stage advanced,the ERCC1 mRNA level declined and the RRM1 mRNA level increased (t=2.679 and 2.952,P =0.034 and 0.025,respectively).Levels of mRNA in patients with complete remission,partial remission,stable disease and progressive disease were (0.487 ± 0.097,0.511 ± 0.095,0.552 ± 0.086,0.568 ± 0.088) for ERCC1 and(0.504±0.091,0.544±0.095,0.595±0.093,0.616±0.097) for RRM1,respectively.The clinical effect of chemotherapy was negatively correlated with mRNA expression of ERCC1 and RRM1 (r=0.567,P=0.032).Conclusions Levels of ERCC1 and RRM1 mRNA expression are correlated with the staging of esophageal squamous cell carcinoma and chemotherapy sensitivity,and can be used as a predictive parameter for chemotherapy sensitivity in patients with esophageal squamous cell carcinoma.

Objective To detect the expression of kisspeptin-1 (KISS-1),matrix metallopmteinase-2 (MMP-2) and vascular endothelial growth factor (VEGF) in the tissue of colon cancer,and analyze the relativity between KISS-1,MMP-2,VEGF and pathological characteristics of colon cancer.Methods A total of 60 colon cancer patients and 60 patients with benign colorectal disease who received surgical treatment in our hospital from January 2009 to June 2010 were selected as observation group and control group respectively.The cancer tissue samples and excision samples collected from them were used to detect KISS-1,MMP-2 and VEGF with immunohistochemistry.Results The positive rates of KISS-l,MMP-2 and VEGF were 31.7％,58.3％ and 78.3％ in observation group,and 73.3％,16.7％ and 33.3％ in control group.The positive rate of KISS-1 in observation group was lower than that in control group (x2=23.489,P＜0.001),and the positive rates of MMP-2 and VEGF in observation group were higher than those in control group (x2=27.469,P＜ 0.001;x2=25.817,P＜0.001).The expressions of KISS-1,MMP-2 and VEGF were significantly related with the histological grade and TNM stage of colon cancer (x2=8.997,P=0.011;x2=6.163,P=0.013;x2=8.519,P=0.014;x2=9.160,P=0.002;x2=16.577,P＜0.001;x2=10.523,P=0.001).Conclusion It is helpful to understand the differentiation and clinical stage of colon cancer and provide evidence for clinical diagnosis and prognosis prediction by detecting KISS-1,MMP-2 and VEGE

Objective:To investigate the first aid management, doctors′ command of first aid knowledge of private dental clinics in Zhangjiakou city and the influencing factors, for references in strengthening the emergency management level of these clinics and the emergency capacity of doctors.Methods:Customized questionnaire was used in a survey of 192 doctors in 64 private dental clinics in the city from June to September in 2019, and 172 valid responses were recovered. The questionnaire covered such aspects as basics of these doctors, first-aid training received and first-aid knowledge command, as well as first-aid management of these clinics. Enumeration data were indicated with frequency number and percentage, while measurement data were indicated with ± s. Univariate analysis of first-aid knowledge scoring was made with Mann-Whitney U test. Results:The first-aid knowledge of dental doctors in private dental clinics in Zhangjiakou was poor, scoring 51.8±14.6 points in general, and those scoring above 60 points accounted for merely 19.3%. The proportion of dental doctors participating in first-aid knowledge training and emergency drill was low. Only 7.8% of the private dental clinics in Zhangjiakou had complete rescue equipment. Scoring of the dental doctors participating in emergency drill and emergency knowledge training was higher than those without such experiences, with the difference being statistically significant( P<0.001). Conclusions:Private dental clinics should regularly arrange their staff to participate in first-aid training and emergency drills; prepare an emergency plan, equip rescue facilities and first-aid medication; dental doctors should strengthen their study of first-aid knowledge.

Objective: To observe the effect of action observation combined with occupational therapy on the upper limb function and ability in the activities of daily living after a stroke. Methods: Forty-two stroke survivors were randomly divided into a treatment group and a control group, each of 21. Both groups were given conventional rehabilitation training and conventional occupational therapy. The treatment group additionally performed action observation. The Fugl-Meyer assessment (FMA-UE), a Hong Kong version of the functional test for the hemiplegic upper extremity (FTHUE-HK) and the Modified Barthel index (MBI) were used for assessment before and after 8 weeks of treatment. Results: Before the treatment there were no significant differences between the two groups. Afterward, significant improvement was observed in all of the measurements in both groups. Compared with the control group, the treatment group showed more significant improvement in its average FMA-UE, FTHUE-HK and MBI scores. Conclusion Action observation can improve the effects of rehabilitation and occupational therapy after stroke.