1.Transcript levels and functional analysis of DNA methyltransferases in CD4+ T cells of MRL/lpr mice
Min YUAN ; Lijuan MU ; Hanjuan CUI ; Xiaobing LUO ; Xinfang HUANG ; Yijun ZHENG ; Yuanjia TANG ; Nan SHEN
Chinese Journal of Rheumatology 2009;13(8):521-524
Objective To investigate the expression levels of DNA methyltransferases 1, 3A and 3B in CD4+ T cells of MRL/lpr mice, and explore their relationship with the expression levels of methylation-sensitive genes (ITGAL, CD70). Methods CD4+ T ceils were isolated from spleens of 16-week-old MRL/lpr and BALB/c control mice by anti-CIM antibody labeled magnetic beads. Transcription levels of DNA methyhransferases 1, 3A and 3B and methylation-sensitive genes(ITGAL, CD70) were measured by real-time mice when compared with BALB/c control mice and the difference was significant (P<0.05), while the expression of DNMTI and DNMT3A showed a tendency of decrease (P>0.05). The mRNA expression of CD70 was significantly higher (P<0.01), but the expression of ITGAL had no significant difference between the two P<0.01 ). Conclusion Decreased expression of DNMT3B may attribute to the elevated expression of methylation-seusitive gene CD70, thus lead to the dysfunction of CD4+ T cell and play an important role in the pathogenesis of SLE.
2.Occurrence and Molecular Identification of Giardia duodenalis from Stray Cats in Guangzhou, Southern China.
Guochao ZHENG ; Wei HU ; Yuanjia LIU ; Qin LUO ; Liping TAN ; Guoqing LI
The Korean Journal of Parasitology 2015;53(1):119-124
The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), beta-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.
Animals
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Cat Diseases/*parasitology
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Cats
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China
;
Cluster Analysis
;
DNA, Protozoan/chemistry/genetics
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DNA, Ribosomal/chemistry/genetics
;
Feces/parasitology
;
Giardia lamblia/*classification/cytology/genetics/*isolation & purification
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Giardiasis/parasitology/*veterinary
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Microscopy
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Molecular Sequence Data
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Phylogeny
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Protozoan Proteins/genetics
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RNA, Ribosomal, 18S/genetics
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Sequence Analysis, DNA
3.Occurrence and Molecular Identification of Giardia duodenalis from Stray Cats in Guangzhou, Southern China.
Guochao ZHENG ; Wei HU ; Yuanjia LIU ; Qin LUO ; Liping TAN ; Guoqing LI
The Korean Journal of Parasitology 2015;53(1):119-124
The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), beta-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.
Animals
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Cat Diseases/*parasitology
;
Cats
;
China
;
Cluster Analysis
;
DNA, Protozoan/chemistry/genetics
;
DNA, Ribosomal/chemistry/genetics
;
Feces/parasitology
;
Giardia lamblia/*classification/cytology/genetics/*isolation & purification
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Giardiasis/parasitology/*veterinary
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Microscopy
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Molecular Sequence Data
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Phylogeny
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Protozoan Proteins/genetics
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RNA, Ribosomal, 18S/genetics
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Sequence Analysis, DNA
4.Recent advance in role of prefrontal cortex in pathogenesis of schizophrenia
Jinglan YAN ; Xiaorong ZHENG ; Yuanjia ZHENG ; Jiang HE ; Yongjun CHEN
Chinese Journal of Neuromedicine 2019;18(2):194-198
Schizophrenia is a serious neurological disorder characterized by incoordination among thinking, cognition, emotion and behavior, and its pathogenesis remains unclear now. In recent years, an increasing number of reports have found that the occurrence of schizophrenia shares a close relationship with prefrontal cortex (PFC), while the later plays an important role in numerous advanced cognitive functions, including working memory, learning and decision making. This review focuses on the recent development of neural circuits, including neurodevelopment, neurotransmitters, synaptic transmission, functional connectivity of brain regions and neural synchrony oscillation in PFC. Hopefully, this review will help to elucidate the pathogenesis of schizophrenia, and provide approaches and ideas for the treatment of relevant psychiatric disorders.
5.Genotyping of Giardia duodenalis Isolates from Dogs in Guangdong, China Based on Multi-Locus Sequence.
Guochao ZHENG ; Muhamd ALSARAKIBI ; Yuanjia LIU ; Wei HU ; Qin LUO ; Liping TAN ; Guoqing LI
The Korean Journal of Parasitology 2014;52(3):299-304
This study aimed to identify the assemblages (or subassemblages) of Giardia duodenalis by using normal or nested PCR based on 4 genetic loci: glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), beta-giardin (bg), and small subunit ribosomal DNA (18S rRNA) genes. For this work, a total of 216 dogs' fecal samples were collected in Guangdong, China. The phylogenetic trees were constructed with MEGA5.2 by using the neighbor-joining method. Results showed that 9.7% (21/216) samples were found to be positive; moreover, 10 samples were single infection (7 isolates assemblage A, 2 isolates assemblage C, and 1 isolate assemblage D) and 11 samples were mixed infections where assemblage A was predominant, which was potentially zoonotic. These findings showed that most of the dogs in Guangdong were infected or mixed-infected with assemblage A, and multi-locus sequence typing could be the best selection for the genotype analysis of dog-derived Giardia isolates.
Animals
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China
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Cluster Analysis
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Coinfection/parasitology/veterinary
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Cytoskeletal Proteins/genetics
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DNA, Protozoan/chemistry/genetics
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Dog Diseases/parasitology
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Dogs
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Genotype
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Giardia lamblia/*classification/*genetics/isolation & purification
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Giardiasis/parasitology/*veterinary
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Glutamate Dehydrogenase/genetics
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Molecular Sequence Data
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*Multilocus Sequence Typing
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Phylogeny
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Polymerase Chain Reaction
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RNA, Ribosomal, 18S/genetics
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Triose-Phosphate Isomerase/genetics
6.Next generation sequencing analysis of drug metabolism related IncRNA in children after living donor liver transplantation
Tian QIN ; Xiangqian GU ; Jianxin ZHENG ; Jinchuan LIU ; Yuanjia TANG ; Feng XUE ; Qiang XIA
Chinese Journal of Organ Transplantation 2019;40(4):219-225
Objective To employ high-throughput next generation sequencing (NGS) for analyzing the expression of lneRNAs and mRNAs in donor samples from pediatric living donor liver transplantation and search differentially expressed lncRNAs and drag metabolic gene for individualized guidance of immunosuppressive agents.Methods Between October 2016 and December 2017,10 liver tissue specimens from living donor liver transplantation children were collected and divided into fast and slow metabolic groups (n =5 each) according to the postoperative profiles of drug metabolism.Samples were assayed for high-throughput NGS.Target analysis was used for functional pathways and screening target genes prediction.Results There were differentially expressed 908 mRNAs and 1228 lncRNAs between slow metabolic and fast metabolic groups (P<0.05).According to the abundance and difference,22 up-regulated and 18 down-regulated mRNAs,13 up-regulated and 24 down-regulated lncRNAs were selected.In addition to CYP3A5,CYP2C19,CYP1A2 and UGT1A1 might affect the metabolism of tacrolimus.At the same time,NONHSAT108617.2 in differemially expressed lncRNAs might regulate the expression of CYP3A5 gene.Conclusions This study has comprehensively analyzed the expression of lncRNAs in donor liver from pediatric liver transplantation.Some differentially expressed drug metabolism related genes may affect tacrolimus metabolism in vivo and thus the postoperative use of immunosuppressive drugs.