Objective To establish a rapid and accurate analysis method for food-derived ACE inhibitory peptides activity in vitro.Methods Reaction time of ACE and substrate was by measuring the hippuric acid liberated in the ACE reaction mixture at regular intervals;An optimal RP-HPLC method to measure food-derived ACE inhibitory peptides activity in vitro was set up.The hippuric acid from ACE reaction mixture(sea cucumber peptides were regarded as ACE inhibitor) was estimated by Zorbax SB-C_(18) analytical column with acetonitrile and ultrapure water as mobile phase.Results The reaction time of ACE with substrate was determined at sixty minutes;The elution was carried out with the ratio of acetonitrile to ultrapure water was 1:1(0.1%TFA) at a flow rate of 0.4 mL?min~(-1).The ahsorbance of the eluent was monitored at 228 nm,and column temperature was 25℃.The relationship between hippuric acid concentration and peak area exhibited a good linearity in the concentration ranges of 0～200?g?mL~(-1) and 200～800?g?mL~(-1).The RP-HPLC method was further validated by captopril,the oyster hydrolysate and the anchovy hydrolysate.Conclusion The method has been proved to be convenient,accurate and suitable for the analysis of foodderived ACE inhibitory peptides activity in vitro.

This article mainly elaborated around the four common categories of objective classification indicators, including constitution-gene and pathway, symptoms and signs, imaging examinations and biological indicators. We summarized advantages and limitations in all the objective classification categories, and put forward that both human secretory immunoglobulins A and salivary cortisol have the potential to be important classification indicators in constitution of traditional Chinese medicine, which might bring objective and quantitative criterion for constitution recognition and constitutional interventions in the future.

Objective To evaluate the prognostic values of common definition compared to traditional definition of contrast-induced nephropathy (CIN) in patients with normal serum creatinine (SCr). Methods Patients undergoing percutaneous coronary angiology or intervention with normal baseline SCr were enrolled prospectively. Those who were diagnosed as CIN according to common definition were divided into two groups based on the peak increase from baseline in the SCr concentration within 48 ~ 72 hours after the procedure: ≥ 44.2 μmol/L (CIN44.2 group, in common with traditional definition), ≥25% of baseline to < 44.2 μmol/L (CIN25%-44.2 group, interval between the two definitions). Hospital stay and long-term outcomes were compared among CIN44.2, CIN25%-44.2, and non-CIN groups. Results Of all 3,044 patients enrolled, 302 (9.9%) patients developed CIN according to common definition including CIN44.2 occurred in 56 (1.8%) patients and CIN25%-44.2 in 246 (8.1%) patients. Patients in CIN44.2 group indicated significant longer hospital stay and long-term outcomes compared with non-CIN group (P < 0.05). However, patients in CIN25%-44.2 group had similar in-hospital mortality and long-term cumulative risk of major clinical adverse events (MACE) and death with non-CIN group (all, P = 1.00). Multivariate Cox proportional hazard analyses also demonstrated that CIN25%-44.2 did not associate with long-term MACE (HR 1.16, P = 0.645) and death (HR 0.98, P = 0.964) after adjusting for potential confounding factors. Conclusions For patients with normal baseline SCr, common definition based on traditional definition of CIN is unreasonable and overestimates the incidence of CIN, whose extension of traditional denifition proves no significant clinical value.

Objective:To study the anti-inflammatory and analgesic effects of different solvent extracts of Folium Pyrrosiae. Meth-ods:Water extract and 75% alcohol extract of Folium Pyrrosiae were obtained. Mouse auricle swelling model induced by xylene was used to observe the anti-inflammation. The analgesic effect was tested by acetic acid writhing test and hot plate test. Results:The eth-anol extract of Folium Pyrrosiae could markedly inhibit the mouse auricle swelling induced by xylene (P<0. 01), and had the ability to inhibit the twisting induced by acetic acid in the mice (P <0. 05). The ethanol extract of Folium Pyrrosiae could increase the threshold of pain in the mice significantly after the 1-hour and 2-hour treatment (P<0. 05). The water extract of Folium Pyrrosiae could inhibit the mouse auricle swelling induced by xylene and the writhing reaction induced by acetic acid (P<0. 05). The water ex-tract of Folium Pyrrosiae could increase the threshold of pain in the mice significantly after the 1-hour treatment (P<0. 05). Conclu-sion:Folium Pyrrosiae has obvious analgesic and anti-inflammatory effects.

Objective To explore the influence of organizational safety atmosphere on work enthusiasm among junior nurses in ICU,so as to provide reference for improving job engagement and work quality of junior nurses in ICU. Methods In December 2016,213 junior nurses in ICU from 12 comprehensive class Ⅲhospitals in Nanning,Guilin and Liuzhou in Guangxi were selected and surveyed by the Safety Organizing Scale(Chinese version)and Questionnaire on Work Enthusiasm Appraisal. Influence of organizational safety atmosphere perceived by junior nurses in ICU on their work enthusiasm was analyzed. Results For the junior nurses in ICU,scores of safety organizing were(38.60±7.12)points,and scores of work enthusiasm were (57.04±8.23);differences of junior nurses' work enthusiasm scores under different organizational safety atmosphere were statistically significant(F=10.908,P< 0.001). Work enthusiasm and its dimensions were positively related to organizational safety atmosphere(P<0.05). Conclusions The more safety organizing investment is,the higher work enthusiasm is of junior nurses in ICU.

Objective? To analyze the bias of dimension measurement results of penis and testis by the self-design cylinder boy penis and testicles measurement (hereinafter called cylinder ruler) and it influencing factors. Methods? From June to December 2018, we selected 152 children of Department of Urinary Surgery in Hu'nan Children's Hospital as subjects by case sampling. Children's penis and testicles dimension were measured repeatedly by their patients and nurses with the self-designed cylinder ruler and three kinds of common clinical measurement tools (vernier caliper+tape measure+testis measurer, vernier caliper+straightedge, vernier caliper+round head probe), and the bias were counted based on medical reference value. Results? Measurement bias of length and diameter of penis in a resting state, length of penis in a traction state, length and width of testis in group of cylinder ruler were lower than those in other groups with statistical differences (P＜0.05) which not influenced by boys' ages and measurement time (P＞ 0.05). Bias of length of penis in a traction state, length and width of testis measured by nurses were lower than those by children's patients with statistical differences (P＜ 0.05). Interaction effect analysis showed that there were no statistical differences in dimension of penis and testis measured by different measurers with the cylinder ruler (P＞0.05); however, there were statistical differences in dimension of penis and testis measured with the cylinder ruler by different measurers at different times of a day (P＜0.05). The differences of interaction effects between measurement time and measurers were statistical (P＜0.05). Conclusions? Compared with other measurements, the self-designed cylinder ruler for measurement of penis and testis in boys has higher accuracy. The main influencing factors of bias of dimension of boys' penis and testis are ages of boys and measurement time. Bias of length of penis in a traction state, length and width of testis measured by nurses are lower than those by children's patients. Measurers and measurement time are not suggested as independent factors when analyzing the influencing factors of dimensions measurement bias of penis and testis. Dimensions of penis and testis are measured with the cylinder ruler at different times which have certain changes.