To establish the quality standards, the authors identified Semen Arecae and Pericarpium Citri Reticulatae in Xiaoerxiaoshi Tablets with TLC and determined the content of ursolic acid and organic acids with TLC-colorimetry and neutralization method.

Objective To study the influence of progesterone (PROG) on infarct volume and functional outcome and to evaluate the therapeutic value of PROG on cerebral infarction in rats.Methods Health adult male Sprague-Dawley rats were randomly divided into sham-operated (control) group,ischemic group,vehicle-treated group and PROG-treated group.Permanent cerebral ischemia was induced by occlusion of the left middle cerebral artery (MCA) using an intraluminal filament technique.Sham-operated rats were subjected to the same surgical procedure,except that the filament was not advanced to occlude the MCA.Progesterone or 2-hydroxypropyl-β-cyclodextrin was injected intraperitoneally following permanent middle cerebral artery occlusion (PMCAO) of rats.Zea Longa test was used to evaluate their functional outcome at 1d,2d,3d after stroke.TTC staining was used to detect the infarct volume at 3d after stroke.Results The results of Zea Longa test showed that there were no functional deficits in all animals prior to ischemia.There were no significant changes in motor function in sham-operated animals across the 3 days assessment period.Both PROG and vehicle-treated rats experienced significant decline in scores following occlusion.However,PROG-treated rats (3.00 ± 0.63,2.83 ± 0.75,2.00 ± 0.89 )demonstrated a gradual improvement in scores compared with ischemic (4.00 ± 0.89,3.83 ± 0.75,3.16 ± 0.75 )and vehicle-treated rats ( 3.67 ± 1.21,3.50 ± 1.05,2.83 ± 0.76) at different times (P ＜ 0.05 ).TTC staining revealed that PROG administration significantly reduced the total infarct volume in the PROG-treated rats ( ( 15.03± 3.75) ％ ) compared with ischemic ( (23.74 ± 4.48 ) ％ ) and vehicle-treated rats ( ( 24.42 ± 7.07 ) ％,P ＜0.05).Conclusions PROG significantly reduces infarct volume and promotes the recovery of neurological functions after pMCAO,which has good therapeutic value for the rat model of cerebral infarction.

0.05).A total of 121 patients were involved in present study.Ninety days after treatment the BI scores of group Ⅰ,Ⅱ and Ⅲ were 63.7?12.7,62.3?13.5 and 42.6?13.1,respectively,and the significant differences existed between group I and group Ⅲ(P0.05).Compared with the baseline data,the fibrinogen of group Ⅰ and Ⅱ decreased at day 3,5 and 7(P

ObjectiveTo study the relationship between the plasma level of asymmetric dimethylarginine (ADMA) and the damage degree of white matter and the changes of cognitive function in patients with cerebral small vessel disease.Methods120 patients with white matter lesions,induced by cerebral small vessel disease,were randomly selected.All patients were divided into group with or without cognitive dysfunction according to the results of MoCA scale.The plasma level of ADMA was detected with high-perfomance liquid chromatography method.Scheltens semi-quantitative evaluation was used to detect the damage degree of white matter.Correlation analysis was used to evaluate the relationship between the plasma level of ADMA and white matter lesions and cognitive dysfunction.ResultsThere were 42 patients with cognitive dysfunction and 78 patients without cognitive dysfunction.The plasma level of ADMA in patients with cognitive dysfunction ( ( 1.57 ±0.56) μg/ml) was significantly higher than patients without cognitive dysfunction ( (0.89 ±0.23 ) μg/ml) (P ＜ 0.01 ).Scheltens semi-quantitative evaluationfor patients with cognitive dysfunction and patients without cognitive dysfunction was 8.01 ± 2.50and 2.49 ± 0.59,respectively.There was significant difference between this two groups (P ＜ 0.01 ).The plasma level of ADMA positively correlated with the damage degree of white matter lesions and the changes of cognitive function.The correlation coefficients were 0.913 and 0.837,respectively ( All P ＜ 0.01 ).ConclusionADMA may be involved in the process of white matter damage caused by cerebral small vessel disease.The detection for plasma levels of ADMA may contribute to the prevention and early diagnosis of patients with cognitive dysfunction caused by cerebral small vessel disease.

Objective To explore common risk factors of the first acute ischemic cerebral stroke patients′neurological deficits and build a short-term prognosis model. Methods 89 hospitalized patients with acute is-chemic cerebral stroke were chosen for study from September 2014 to December 2015 in the Fifth Affiliated Hos-pital of Zhengzhou University. Our study′s evaluation methods were using the unified questionnaires , the NIHSS score and the mRS scale. Results Traditional risk factors were no significant difference among the three groups (P > 0.05); 6 kinds of hematology indexes such as WBC count had significant difference in NIHSS score (P <0.05) and prognosis(P < 0.05); 6 kinds of hematology indexes such as D-D and the NIHSS score had a signifi-cant effect on prognosis (OR = 1.800, 0.976, 1.112, 1.327, 5.564, 6.456, 1.227); the area under ROC curve was 0.976, which proved the model had a good predictive value. Conclusion Traditional risk factors had no significant difference among the different neurological deficits groups; 6 kinds of hematology indexes such as D-D and NIHSS score on admission had a significant influence on prognosis; the model predicted the short-term prognosis of acute ischemic cerebral stroke more accurately.

Objective To observe the efficacy of intense pulsed light(IPL)incorporated with optimal pulse technology (OPT)in the treatment of rosacea.Methods Thiris-two patients with erythematotelangiectatic rosacea and 53 patients with papulopustular rosacea were treated with IPL-OPT for 4 sessions with an interval of 3 weeks.Patients were assessed clinically and photographically by physicians before each treatment.Skin melanin index,erythema index,sebum secretion level and water content in stratum corneum were tested at the baseline,3 weeks after each treatment,and 6 months after the last treatment.Results Three weeks after the last treatment,the effective(more than 60%improvement)rate WaS 81.18%in total,75%in erythematotelangiectatic rosacea,and 84.91%in papulopustular rosacea;there WaS no significant difference between erythematotelangiectatic rosacea and papulopustular rosacea (x2=1.28,P＞0.05).Six months after the last treatment,the total effective rate still remained at 78.82%with no significant difierence from that observed at 3 weeks after the treatment(x2=1.62,P＞0.05).The value of melanin index,erythema index and sebum secretion level decreased significantly after the final treatment,however,water content in stratum corneum remained at the salne level as that before treatment.After 6-month follow-up,no significant change was noticed in the above 4 parameters compared with those obtained at 3 weeks after the treatment.Neither hyperpigmentation nor hypopigmentation was observed during the treatment and follow-up.Conclusion This study demonstrates that IPL-OPT is an effective treatment for rosacea with relatively few side effects.

OBJECTIVE To learn the classification of candidal hospital infection and its antifungal drug susceptibility test,in search for prevention and treatment measure.METHODS The CHROMagar candida color medium was applied to isolate Candida,DADE WalkAway-40 and RY-ID were applied to identify Candida strains.Rosco disc diffusion method was applied to analyze susceptibility test of Candida strains.RESULTS Of the 702 isolates,70.0% were C.albicans,20.8% were C.glabrata,and 6.3% were C.tropicalis.The candidas showed various resistance to 6 anti-fungal drugs.CONCLUSIONS C.albicans is still the most common pathogen of candidal infection,but in other candida the percentage of C.glabrata is rising now.Amphotericin B,fluconazole,itraconazole and nystatin(Anticandine) have a good antifungal to all candidas,but flucytosine and ketoconazole have less susceptibility to them.So medication should be accorded with the results of antifungal susceptibility test.

This study was aimed to establish a method for assay of 4-terpineol, α-terpineol, bornyl acetate and methyl-n-nonylketone in Houttuynia cordata with gas chromatography-mass spectrometry (GS/MS) for the first time in order to provide experimental evidences for its quality control. Undecylen was analyzed quantitatively. GC/MS method was used in the content determination of 4 kinds of components in Houttuynia cordata. GC conditions were that the temperature was firstly increased to 70oC for 5 min, and then 5oC·min-1 to 140oC for 5 min. Then, the temperature was increased by 10oC·min-1 to 250oC for 10 min. The temperature of the injection port was 230oC. The carrier gas was high purity helium. The flow rate was 1.0 mL·min-1. The split ratio was 10:1. The sample quantity was 1 μl. MS conditions were that the detector was MS, EI was 70ev, the temperature of four poles was 150oC, the temperature of ion source was 230oC. The results showed that there was good linearity of four components, which were 4-terpineol, α-terpineol, bornyl acetate and methyl-n-nonylketone obtained within the ranges of 6.492~129.84μg·min-1, 1.097~21.944 μg·min-1, 12.128~242.56 μg·min-1, and 84.76~169.52 μg·min-1, respectively. The average recoveries of the four components were 81.2%, 80.6%, 88.3%, and 84.6%, respectively. The RSDs were 1.5%, 2.3%, 1.1%, and 0.7%, respectively. It was concluded that the method was simple, sensitive, and easy to operate. The simultaneous measurement of multiple indexes on the component for quality evaluation of Houttuynia cordata can effectively reflect the quality of Houttuynia cordata. It can also be used in the quality assessment of fresh Houttuynia c ordate .

Objective To find out the resistant situation and drug of Mycobacteria patients in Sichuan and offer foundation for clinical.Methods Two hundred randomized clinical isolates of Mycobacterium were determined by Roche drug sensitivity and minimum inhibitory concentration (MIC) method.Results Of the 200 clinical isolates,192 stains were Mycobacterium tuberculosis(MTB) (96.0％),8 strains (4.0％) were non-tuberculosis mycobacterium(NTM).Of the 192 MTB strains,108( 57.3％ ) sensitive strains and 84 (43.7％)stains were resistant to one or more than one drugs.Among these 84 resistant strains 23 were multi-drug resistant ( MDR,12.0％ ),4 were extensively drug resistant( XDR,2.1％ ).The anti-TB drug resistance rates were:SM(16.7％),INH(20.8％),RFP(17.2％),EMB(10.9％),PI(16.1％),LFX(8.8％),AMK ( 16.7％ ),CPM ( 6.2％ ),PTA ( 33.3％ ),respectively.Conclusion The resistance rate of tuberculosis keeps at a high level in Sichuan,especially the resistance rate of multiple (≥4) drug,we should oar attention.

Objective To analyze the possible association of psoriasis vulgaris with herpes simplex virus type 1 (HSV-1). Methods Polymerase chain reaction was used to detect HSV-1 DNA in lesional skin biopsies, periphery blood mononuclear cells (PBMCs)and throat swabs from patients with psoriasis vulgaris, and ELISA was used to detect IgM and IgG antibodies against HSV-1 in sera from these patients. Results The positive detection rates of HSV 1 DNA in lesional skin biopsies, PBMCs and throat swabs were 37.5%, 18.6%and 18.8%, respectively. Anti HSV 1 IgM and IgG antibodies were positive in 37.2%and 53.5%of serum specimens, respectively. The detection rates of HSV 1 DNA in lesional skin biopsies and PBMCs, and IgM antibody in sera were significantly higher than those in normal controls. In psoriatic patients of guttate type the positive detection rates of HSV 1 DNA and IgM antibody were significantly higher than those in the plaque type. Conclusions There is strong association of psoriasis vulgaris, especially the guttate type, with HSV 1, and there may be recent infection of HSV 1 in these patients.