OBJECTIVE: To investigate the effects of Gypsum Fibrosum and Gypsum Fibrosum Preparatum in promoting granulation. METHODS: The wounds of muscle layer were produced in rats by using surgical operation. Two round wounds, with diameter about 1.5 cm, were cut at the depilatory area of two sides of the back of each rat, with an interval of 2 cm, deep to muscle layer, and the thickness of the knife wound of muscle layer was about 0.15 cm. Forty SD rats with the wounds were randomly divided into 4 groups: untreated group, beifuji-treated group, Gypsum Fibrosum-treated group and Gypsum Fibrosum Preparatum-treated group, with 10 rats in each group. Then the wounds were sprinkled with powders of Gypsum Fibrosum and Gypsum Fibrosum Preparatum, or sprayed with beifuji solution, respectively. The healing state of granulation tissues of the wounds was observed at the eighth and fourteenth day respectively. RESULTS: The number of fibroblasts, the number of capillary tubes and the area of capillary tubes in granulation tissue of wounds in the Gypsum Fibrosum Preparatum-treated group were significantly higher than those in the untreated group and Gypsum Fibrosum-treated group (P<0.01). There were no statistical differences between the Gypsum Fibrosum Preparatum-treated group and the beifuji-treated group. However, Gypsum Fibrosum-treated group showed no obvious differences compared to the untreated group (P>0.05). CONCLUSIONS: Gypsum Fibrosum Preparatum can accelerate the formation of collagenoblast and micrangium in wounds, and the proliferation of granulation tissues, thus promoting the skin wounds to healing. The effect of Gypsum Fibrosum is changed after being calcined, and Gypsum Fibrosum Preparatum has obvious effect in promoting granulation.

Objective To probe into effective surgical methods to reduce post-operational recurrence rate of nodular goiter.Methods One hundred and fifty-seven patients with nodular goiter who were admitted and had operations in our hospitals between Jan,2007 and June,2011 were assigned to one of the following 4 groups:total thyroidectomy(TT),nearly total thyroidectomy (NTT),subtotal thyroidectomy (STT),removal of lesion only or partial thyroidectomy (also termed as local removal,LR).Postsurgical recurrences and complications were compared among these groups.Results The recurrences were statistically analysed.There was a highly significant difference between TF and STT、TT and LR、NTT and LR; no difference was observed between TT and NTT,STT and STT + E,STT and LR.A statistical analysis was also carried out on the postsurgical compications in these patients.highly significant differnce was found between TT and STT,TT and NTT,TT and LR.There was a significant diffrence between STT and NTT.There was no difference between STT and LR,NTT and LR.Conclusion TT,STT,NTT are all effective operations for nodular goiter and should be advocated.Satisfactory efficacy may be obtained with oral thyroxine or euthyrox for postsurgical lifetime.However,LR or partial thyroidectomy should be adopted cautiously.

OBJECTIVETo investigate the changes in serum levels of sex hormones in male workers occupationally exposed to cadmium (Cd).

METHODSEighty-five Cd-exposed workers in a cadmium refinery in the south China and 76 local healthy subjects as control were selected in the study. Air samples in the workplaces were collected and detected for Cd concentration. Urinary Cd (UCd) level of the workers was measured by graphite atomic absorption spectrometry (AAS) and adjusted by urine level of creatinine (Cr), as an indicator of Cd-burden in the body of all subjects. Also, their serum levels of testosterone (T), follicle stimulating hormone (FSH) and luteinizing hormone (LH) were determined with radioimmunoassay and enzyme immunoassay, respectively, and dose-effect relationship was evaluated.

RESULTSThe serum testosterone levels in Cd-exposure group with 10.9-21.9 and > 21.9 micro mol/mol Cr were 13.00 and 11.37 nmol/L, significantly higher than that (9.31 nmol/L) in those with 0.0-2.2 micro mol/mol Cr. Significantly more increased level of LH (4.11 and 4.32 U/L) was detected in heavy exposure group in the workshop for electrolysis than in control group (2.52 U/L) and in the group with 0.0-2.2 micro mol/mol Cr of UCd (2.64 U/L). No changes in serum level of FSH were found related to Cd exposure.

CONCLUSIONOccupational Cd exposure could independently contribute to the changes of serum levels of sex hormone in male workers.

Cadmium ; adverse effects ; Follicle Stimulating Hormone ; blood ; Gonadal Steroid Hormones ; blood ; Humans ; Luteinizing Hormone ; blood ; Male ; Occupational Exposure ; adverse effects ; Testosterone ; blood

OBJECTIVETo investigate the possible effects of oral cadmium(Cd) exposure on the expression levels of metallothionein-I and metallothionein-II (MT-I and MT-II) mRNA and the distribution of zinc (Zn) and Cd in rat prostate.

METHODSCadmium was given to rats at doses of 50, 100, and 200 mg/kg in drinking water. The contents of Zn and Cd in prostate were measured by atomic absorption spectrometry(AAS), and the levels of MTs mRNA were determined by RT-PCR.

RESULTSAfter Cd administration, the content of Zn was decreased in both the ventral and the dorsolateral lobes of rat prostate. In 200 mg/kg Cd group, the contents of Zn in ventral prostate were 9.5 and 8.5 micrograms/g wet weight respectively after one month and six months, which were significantly lower than those of control(17.0 and 18.9 micrograms/g wet weight). In contrast, the contents of Cd in both ventral and dorsolateral lobes of prostate significantly increased with the increasing dose and time of Cd administration. It was also noted that Cd administration resulted in a significant down-regulation of the expression of MT-I and MT-II mRNA in rat ventral prostate. In 200 mg/kg Cd group after one and six months, the relative expression levels of MT-I (0.410, 0.339 respectively) and MT-II (0.100, 0.112 respectively) were significantly lower than those of MT-I (0.760, 0.830 respectively) and MT-II (0.429, 0.439 respectively) in control group.

CONCLUSIONOral Cd exposure could influence the distribution of Zn and the expression levels of MTs mRNA in rat prostate.

Administration, Oral ; Animals ; Cadmium ; metabolism ; toxicity ; Dose-Response Relationship, Drug ; Male ; Metallothionein ; genetics ; Prostate ; drug effects ; metabolism ; RNA, Messenger ; analysis ; Rats ; Zinc ; metabolism

Objective:To prepare recombinant protein of group 36 allergen of Dermatophagoides farinae(Der f 36),to deter-mine its immunogenicity and bioinformatics analysis was performed.Methods:Nucleic acid sequence coding for Der f 36 was obtained and artificially synthesized,pET-28a(+)was inserted to construct pET-28a(+)-Der f 36 plasmid and transformed into BL21(DE3)receptor cells.After expressed and purified in BL21(DE3),recombinant allergen rDer f 36 was obtained,recombinant protein rDer f 36 was identified by SDS-PAGE and Western blot.Serum IgE binding rates of rDer f 36 was determined by IgE-ELISA.Cross-reactivity between rDer f 36 and rDer p 36 was detected by IgE-ELISA inhibition assay.HNEpC cells were cultured with rDer f 36 for 24 h and cytokines were detected.Bioinformatics softwares were used to analyze physicochemical properties and structures of Der f 36 and Der p 36.Results:Coding gene for Der f 36 was obtained with a total length of 690 bp,whose molecular weight was 25.6 kD;serum IgE binding rate of rDer f 36 was 42.1%by IgE-ELISA;IgE-ELISA inhibition assay showed that rDer p 36 had 40%(8/20)inhibition rate(>50%)for rDer f 36,with an average inhibition rate of 52.98%.Compared with control group,HNEpC cells cultured with rDer f 36 showed that IL-6,IL-8,IL-33,IL-25 and TSLP expressions had increased;bioinformatics analyses show that sequence consistency of Der f 36 and Der p 36 was 77.63%,with similar physicochemical properties.Secondary structure analysis showed that both of them contained α helix,β-turn and random coils,and content of random coils was the highest;structure of C2 domains was also highly overlapping(RMSD=0.046).Conclusion:Recombinant allergen rDer f 36 is successfully prepared with good immunogenicity,laying foundation for diagnosis and treatment of dust mite allergen single component.High similarity in physical and chemical properties,secondary structure,and tertiary structure between Der f 36 and Der p 36 determines their cross reactivity.

Objective:To prepare recombinant protein of group 27 allergen of Dermatophagoides farinae(Der f 27),and to determine its immunoactivity.Methods:pET-28a(+)-Der f 27 plasmid was constructed and inserted into E.coli BL21(DE3)cells.After being expressed and purified,recombinant allergen rDer f 27 was obtained.IgE binding rates of rDer f 27 with sera from patients with allergic rhinitis induced by Dermatophagoides farinae was determined by ELISA and Western blot.PBMC from patients with allergic rhinitis and BESA-2B cells were cultured with rDer f 27 for 24 h,respectively,and cytokine expression was measured.Bioinformatics softwares were used to analyze physicochemical properties and structures of Der f 27.Results:pET-28a(+)-Der f 27 plasmids were prepared successfully and transformed into BL21(DE3)cells.After expressed and purified with PTG,SDS-PAGE and Western blot identified a band about 48 kD.IgE binding rates of rDer f 27 were 39.5%and 45.5%with sera from patients with allergic rhinitis allergic to Dermatophagoides farinae by IgE-ELISA and IgE-Western blot,respectively.Compared with control group,IL-6 and IL-8 expressions were increased in PBMC from patients with allergic rhinitis being cultured with rDer f 27(P<0.05);expressions of IL-10 and TGF-β were decreased in BESA-2B cells being cultured with rDer f 27,while IL-17A and IL-23A expressions were increased.Bioinformatics analysis showed that Der f 27 belong to serine protease inhibitor family and had universal structure and func-tion of this family.Secondary structure of Der f 27 was mainly composed of α-helix(42.62%)and random coil(35.60%).Conclusion:Recombinant allergen rDer f 27 has been prepared successfully with good immunoreactivity and immunogenicity,becoming one of important allergens of allergic rhinitis.