Objective To perform gene diagnosis of Duchenne muscular dystrophy (DMD) in 3 family members who were negative for DMD gene detected by multiplex PCR and to provide genetic counseling for their family members accordingly .Methods The clinical data and genomic DNA of patients and their family members were collected ,DMD gene mutation were detected by multiplex ligation dependent probe amplification (MLPA) or the 2nd generation of high‐throughput sequencing .Results In the first family ,3 male patients were detected deletion of Exon 7 and 2 female were heterozygous carriers .In the second family ,it was found in the proband that point mutation of c .3127C> T in the Exon 23 of chrX‐32486626 and c .3127C> T heterozygous mutations was confirmed in his mother ,the mother was pregnant with a girl .In the third family ,point mutation of c .2411G>A was detected in the Exon 20 of chrX‐32509581 in the proband and his mother had c .2411G> A heterozygous mutation .Conclusion MLPA or combining with the 2nd generation of high‐throughput sequencing can offer effective gene diagnosis for the patients of DMD and their family members ,and provide the basis for genetic counseling and prenatal diagnosis .

Objective: To analyze the short-term and long-term effects of non-operative therapy in the treatment of prolapsed lumbar intervertebral discs. Methods: One hundred and sixty-five subjects were randomized in single-blind method into traction group (A), Tuina group (B), and Tuina and exercise group (C); after 2 courses of treatment, the short-term effects were evaluated. Moreover, 102 cases with significant short-term effects were randomized in single-blind method into exercise group (D) and control group (E). The relapse rates in the two groups were survey one year after treatment; and the lumbar functions were assessed respectively six months and twelve months after treatment. Results: Groups B and C had obviously better effects than group A (χ2=8.359, P＜0.01); the relapse rate after one year was strikingly lower in group D than group E (χ2=12.631, P＜0.01). Conclusion: Tuina plus functional exercise is an effective method in the prevention and treatment of prolapsed lumbar intervertebral discs.

Seventy-three cases of cervical spondylosis were treated with cervico-occipital traction belt. During the conduction of traction, acupoimts Bailao (Ex-HN), Fengchi (GB 20),Tianzhu (BL 10), Jianjing (GB 21), Tianzong (SI 11) and Shousanli (LI 10) were needled. When needling sensation arrived, moxibustion was at the same time given by igniting moxa around the needles. After two-course treatment, 60 cases got clinical cure, 8 cases got marked effectiveness, 2 cases got effectiveness and 3 cases failed, the total effective rate being 95.9%.

OBJECTIVE:To probe into the characteristics of adverse drug reactions (ADR) occurred in our hospital. METHODS: 340 ADR cases collected from Jan. 2004 to Dec. 2008 in our hospital were analyzed retrospectively. RESULTS: Of the total 340 ADR cases,80 (23.53%) were collected from pharmacy department;63.83% occurred in patients aged from 21 to 60 years,with females showing higher proportion than males;83.24% were induced intravenously,48.08% were induced by anti-infective drugs,of which,cephalosprins and quinolones took the lead. 38.05% of the ADR manifested as lesion of skin and its appendants. CONCLUSION: ADR monitoring should be strengthened in clinic;furthermore,the quality of ADR reporting should be improved and the use of drugs should be rationally in a standard way so as to lessen or avoid the occurrence of ADR.

Objective To investigate serum levels of sialic acids(SA)vs carcino-embryonic antigen(CEA)for the diagnosis of digestive tract cancer.Methods Sixty healthy adults,100 patients with malignant digestive tract tumor and those undergoing radical digestive tract tumor surgery were enrolled in this case-control study.Serum SA level was tested.Serum CEA level was measured by electrochemical luminescence.Sensitivity and specificity of SAvs CEA were calculated.Receiver operating characteristic (ROC)curve and area under the curve(ROC-AUC)were used to evaluate the diagnostic value of SA.Results Serum level of SA of patients with digestive malignant tumors was much higher than the normal controls(P ＜0.01),although serum SA of patients undergoing tumor resection showed no difference with the normal controls(P ＞ 0.05).There was significant difference in SA level between the patients who received surgical therapy or not.The sensitivity and specificity of SA in diagnosing digestive tract cancer was 55.00％ and 93.3％,respectively.The false positive rate was 6.7％ in normal subjects.Conclusion Serum SA or CEA testing may be equally useful in the screening,diagnosis,outcome assessment and followup study of digestive tract cancer.

Purpose: The clinical effects of scalp acupuncture in treating infantile cerebral palsy were observed. Methods: Forty-five patients were treated by scalp acupuncture, functional exercise, intravenous drip,and parents' instructive training. Results: Basic recovery occurred in 4 cases, marked effectiveness in 21 cases and effectiveness in 15 cases. The total effective rate was 88.9%. The shorter duration and the longer course were,the better curative effects got. Conclusion: A combined treatment of scalp acupuncture, physiotherapy and intravenous drip can markedly improve clinical symptoms, signs, and intelligence in children with cerebral palsy.

Objective: To observe the clinical efficacy and safety of early acupuncture in the treatment of acute ischemic stroke. Methods: In clinical randomized controlled trial, 90 subjects were divided into drug group and acupuncture group, 45 cases in each group. The neurological deficit score, Bathel index and some blood laboratory indexes were detected before and after treatment in both groups. Results: In acute stage, there were striking differences in the neurological deficit score and activities of daily living (ADL) between before and after treatments in acupuncture group (P<0.01), and between acupuncture group and drug group (P<0.01); but there was no difference in blood lipid and theology between two groups. Conclusion: Acupuncture is rather safe in the early treatment of acute ischemic stroke, and improvement of disability and the patients' quality of life.

Objective: To observe the clinical effects of a modified Tuina method in the treatment of childhood diarrhea. Methods: Thirty children with diarrhea of spleen deficiency were treated by Four-Step Tuina(treatment group); and control group was treated with routine Tuina methods. The scale of children diarrhea was used as an objective index for therapeutic effect evaluation. Results: After treatment, the scale was 1.4±1.92 in treatment group and 3.3±3.45 in control group; the statistical analysis showed that there was a difference between the scales; the treatment group had better effects than control group, in another word, the Four-Step Tuina was quite effective for children diarrhea. Conclusion: Tuina has positive effects on children diarrhea of spleen deficiency, which provides evidences for the standardization of clinical therapeutic effects.

Forty cases of type Ⅱ diabetes mellitus were treated by puncturing point Neiguan (PC 6), and the effect on their cardiac vegetative nerve functions were observed at 20 min, 40 min and 60 min after acupuncture respectively. The findings showed that all heart rate variables improved remarkably at the three time periods after acupuncture treatment, with significant differences (P＜0.01); but there was no significant difference in the curative effects among the three time periods.

Objective: To investigate the effects of acupuncture and combined acupuncture and medicine on the quality of life and the genital endocrine level in postmenopausal women. Methods:Forty-seven patients were randomly assigned to acupuncture and acupuncture plus medicine groups,23 cases in the acupuncture group and 24 cases in the acupuncture plus medicine group. The observed course of treatment was 3 months. A Chinese concise SF-36 questionnaire was used to evaluate the quality of life in the different groups of patients before and after treatment.Meanwhile, radioimmunoassay was used to observe the differences in serum genital hormones:follicle stimulating hormone (FSH), luteinizing hormone (LH) and estradiol (E2) contents between pretreatment and posttreatment in both groups. Results: After treatment, there was a significant difference in quality of life between the two groups and it was better in the acupuncture plus medicine group than in the acupuncture group(P＜ 0.01). E2 content increased more markedly in the acupuncture plus medicine group than in the acupuncture group(P＜ 0.05). There were no significant differences in FSH and LH contents between the two groups (P ＞ 0. 05). Conclusion: Both acupuncture and acupuncture plus medicine can improve the quality of life and increase obviously low E2 content in postmenopausal women. Acupuncture plus medicine raises the patients' quality of life and E2 content more markedly than acupuncture. It is suggested that both acupuncture and acupuncture plus medicine can improve the symptoms, signs, and physical and mental health, raise the quality of life and ameliorate genital-endocrine environment in postmenopausal women, but a combination of acupuncture and medicine has a better effect.