Objective To study the protective effects of bcl-2 gene against lung ischemia-reperfusion injury in vitro. Methods The lungs of rabbits were harvested respectively and preserved according to the way of lung transplantation. Then the lungs were reperfused using an ex vivo model. The rabbits were divided into 3 groups: control group, 0.9%NaCl was given; group2, Ad/Lac Z and group 3, receiving Ad/s-bcl-2. Agents were given to the rabbits by continuous intravenous infusion 48 hours before operations. Results Ad/s-bcl-2 increased the contents of bcl-2 mRNA in lung tissue, increased lung tissue SOD contents, decreased lung tissue MDA contents, decreased PAP, improved lung edema, reduced incidence of apoptosis and improved lung histological manifestation. These indexes in the Ad/s-bcl-2 group were better than in the groups of 0.9%NaCl and Ad/LacZ. Conclusion The bcl-2 gene amy provide significant protective effects on ischemia-reperfusion injury of the lung.

Objective:To investigate the protective effects of Cordyceps sinensis from Tibet and Qinghai on acute renal injury in-duced by gentamicin sulphate ( GM) . Methods:The rat model of acute renal injury induced by GM was established. The biochemical indicators including blood urea nitrogen ( BUN) and serum creatinine ( Scr) in serum and NAG enzyme and urine total protein in urine were detected. HE staining was used to observe the pathologic changes in kidney. Results:Compared with those in the model group, the levels of BUN and Scr in serum and NAG enzyme and urine total protein in urine were significantly reduced in the Cordyceps sinen-sis groups, and kidney cells necrosis was improved in the Cordyceps sinensis groups at low, medium and high dose (P<0. 05), how-ever, the difference between the Cordyceps sinensis groups with different habitats were not significant (P >0.05). Conclusion:Cordyceps sinensis from Qinghai and Tibet both have protective effect on acute kidney injury induced by GM in rats, and the difference between them is not remarkable.

Objective To study the abdominal perineal resection for rectal cancer and clinical application of pelvic floor recon-struction .Methods 30 cases of rectal cancer patients from 2008-2011 were randomly divided into experimental group and the con-trol group ,30 cases in each group .Experimental group patients by columnar abdominal perineal resection of rectal cancer treatment , postoperative pelvic floor defect for dermal matrix reconstruction .The control group patients for colorectal cancer resection by lapa-rotomy .Results Compared with the traditional open resection ,the operation time ,intraoperative blood loss ,postoperative patholog-ical column T3 N0 M0 positive rate ,incidence of complications and incision aspect of cylindrical abdominal perineal resection were ob-vious advantages ,the difference was statistically significant (P< 0 .05) .Conclusion Compared with Miles surgery ,cylindrical ab-dominal perineal resection for rectal cancer can reduce the incidence of postoperative incisal edge positive rate and bowel perfora -tion .Human decellularized dermal matrix basin redevelopment can significantly reduce the surgical risk and difficulty of surgical op-erations .

Objective: To investigate the effects of major family structure changes on depression, anxiety and stress symptoms of college students, and to provide theoretical basis for mental health promotion and prevention. Methods: A questionnaire survey was conducted among 9 779 college students from 6 universities, including Jiangxi University of Finance and Economics, Shangrao Normal University, Gannan Normal University, Fujian Polytechnic Normal University, and Changjiang University, by using the Depression, Anxiety and Stress Scale 21 Items (DASS 21). Results: The prevalence rates of depression, anxiety and stress symptoms among college students were 27.4%, 42.0% and 17.4%, respectively. Univariate analysis showed that family structure was associated with anxiety and stress symptoms ( χ 2=8.40,13.08, P <0.05). Multivariate Logistic regression analysis showed that specific family structure other than single or two parent family was positively correlated with anxiety( OR =1.89,95% CI =1.05- 3.42 ) and stress symptoms ( OR =2.48, 95% CI =1.36-4.50), family structure changes due to parental divorce was positively correlated with stress symptoms ( OR =1.53,95% CI =1.05-2.20)( P <0.05). Conclusion The occurrence of depression, anxiety and stress symptoms of college students is related to the type of family structure and the changing factors. Colleges should pay more attention to the mental condition of college students with family structure changes, and deliver various mental health promotion services including psychological counseling and health education.

ObjectiveTo design a simple leg-sliding rehabilitation equipment for patients with lower limb dysfunction who need to exercise their leg muscles in bed at the early stage of rehabilitation. MethodsThe mechanism scheme was designed using the crank slider transmission system. The kinematic model was established and the structural dimensions were determined according to the human joint mobility, analysis and verification were then implemented; and the strutural strength of the machanism was verified, and based on the analysis, a protype was built to verify the feasibility of proposed scheme. ResultsThe range of joint activities of the proposed mechanism was in line with the normal human joint activities, the structural strength met the requirements, the prototype operated smoothly, and the actual running speed was basically consistent with the theoretical planning speed. ConclusionThis design could meet the needs of rehabilitation training for bedridden patients with lower limb dysfunction at the early stage of rehabilitation. It has the advantages of small size and light weight, which provides reference value for promoting the development of miniaturized and lightweight lower extremity rehabilitation equipment.