Objective:Combined with the actual work requirements of brucellosis in Qinghai Province, to design a management information system for brucellosis patients, in order to realize the information of diagnosis and treatment of brucellosis patients.Methods:Based on the theory of management information system, according to the functional requirements of the actual work and performance parameters, the general framework of management information system was designed, the establishment of the management information system was gradually completed, and the system was improved through the system testing.Results:The system was easy to use and easy to operate, and patient's detailed information, diagnosis and treatment results and treatment plan could be obtained quickly. At the same time, follow-up, trace investigation, and brucellosis prevention and treatment propaganda could be carried out.Conclusions:The established brucellosis management information system can realize the electronic management of patient information, master the disease development and diagnosis and treatment results of brucellosis patients. Through follow-up investigation, the system can increase active screening and early warning of suspected cases, and provide health education to realize early diagnosis and early treatment of the patients.

BACKGROUND:The production and release of a large amount of inflammatory factors caused by immune system inflammatory response mainly contributes to secondary spinal cord injury. OBJECTIVE:To investigate the effects of umbilical cord Wharton’s jely mesenchymal stem cel transplantation on repair of injured neurological function and expression of inflammatory factors monocyte chemoattractant protein 1 and interleukin 10 in rats with acute spinal cord injury. METHODS: Eighty-one healthy adult male Sprague-Dawley rats were randomly and equaly divided into sham operation, model and cel transplantation groups, with 27 rats per group. Rats in the latter two groups were subjected to hemisection of the spinal cord to establish acute spinal cord injury models. Rat models in the cel transplantation group received umbilical cord Wharton’s jely mesenchymal stem cel injection (1×106)via the tail vein. Rat neurological function was evaluated using the BBB score at different time points after spinal cord injury. The expression of monocyte chemoattractant protein 1 and interleukin 10 in injured spinal cord tissue was detected using ELISA assay at different time points after spinal cord injury. Migration and neuronal differentiation of umbilical cord Wharton’s jely mesenchymal stem cels in the injured spinal cord tissue were determined using immunohistochemical staining method. RESULTS AND CONCLUSION:Compared with the sham operation and model groups, rat neurological function was significantly recovered in the cel transplantation group (P < 0.05). Compared to the model group, monocyte chemoattractant protein 1 level in the serum and monocyte chemoattractant protein 1 mRNA and protein expression in the injured spinal cord tissue were significantly lower (P < 0.05), but interleukin 10 mRNA and protein expression in the injured spinal cord tissue was significantly higher (P < 0.05), in the cel transplantation group. In the cel transplantation group, umbilical cord Wharton’s jely mesenchymal stem cels could migrate to the injured region and express glial fibrilary acidic protein. These findings suggest that umbilical cord Wharton’s jely mesenchymal stem cels promote rat neurological function recovery by regulating the inflammatory response in the injured spinal cord tissue, which is likely to be one of mechanisms by which transplantation of umbilical cord Wharton’s jely mesenchymal stem cels treats spinal cord injury.

Objective:To study the correlation between the quartering of nerve root subsidence sign (NRS) and the cross-sectional area (CSA) of the narrow segment thecal sac in patients with lumbar spinal stenosis (LSS).Methods:The data of 203 LSS patients in the Fourth People′s Hospital of Hengshui from January 2020 to December 2021 were retrospectively analyzed. All patients underwent MRI cross sectional scanning. The patients were divided into positive type a group ( n=62), positive type b group ( n=32), positive type c group ( n=51), and negative group ( n=58) by NRS quartering method. The minimum CSA, median sagittal diameter (PAD), and lateral recess sagittal diameter of each group were compared. The correlation between NRS quartering classification and the minimum CSA and related indicators of lumbar spinal stenosis was analyzed. Results:The minimum CSA, PAD, and sagittal diameter of the lateral recess in the positive a group, positive b group, and positive c group were all smaller than those in the negative group, while the Visual Analogue Scale (VAS) score and Oswestry Disability Index (ODI) were higher than those in the negative group; The minimum CSA, PAD, and sagittal diameter of the lateral recess in the positive b type and positive c type groups were smaller than those in the positive a type group, while the VAS score and ODI index were higher than those in the positive a type group; The minimum CSA, PAD, and sagittal diameter of the lateral recess in the positive c type group were smaller than those in the positive b type group; The VAS score and ODI index were higher than those of the positive b type group; The differences were statistically significant (all P<0.05). 203 patients were divided into 54 normal cases, 58 mild stenosis cases, 49 moderate stenosis cases, and 42 severe stenosis cases based on the minimum CSA. The coincidence rate between negative NRS and minimal CSA diagnosis as normal was 94.44%(51/54), the coincidence rate between positive type a and minimal CSA diagnosis as mild stenosis was 84.48%(49/58), the coincidence rate between positive type b and minimal CSA diagnosis as moderate stenosis was 53.06%(26/49), and the coincidence rate between positive type c and minimal CSA diagnosis as severe stenosis was 90.48%(38/42). Using the kappa consistency test, the kappa value for quantitative diagnosis of minimum CSA stenosis in NRS and LSS patients was 0.743, indicating good consistency. The kappa values for quantitative diagnosis of NRS, sagittal diameter of lateral recess, and PAD stenosis were 0.271 and 0.335, with poor consistency. NRS typing was negatively correlated with CSA and PAD ( r=-0.723, -0.581, all P<0.001), and positively correlated with VAS score and ODI index ( r=0.473, 0.640, all P<0.001). Conclusions:The NRS quartering method has a good consistency in diagnosing the severity of LSS patients and the minimum CSA of stenosis segments, suggesting that the NRS quartering method can better reflect the degree of Spinal stenosis, which can not only be used as an auxiliary indicator for qualitative diagnosis of LSS, but also has a high value in quantitative diagnosis.

Objective To analyze the human brucellosis monitoring results in Gonghe County of Qinghai Province from 2010 to 2016 and grasp the distribution of human brucellosis.Methods Using retrospective analysis method,monitoring questionnaires,laboratory test forms and case questionnaires of brucellosis in Gonghe County,Qinghai Province from 2010 to 2016 were collected,the serological test results of brucellosis were analyzed and the occupational and onset time distribution were described.Results A total of 1 471 people in Gonghe County were investigated,totally infected people was 32,the infection rate was 2.18％,the number of patients was 18,and the prevalence rate was 1.22％.Eighteen new cases were confirmed.The occupational distribution of infected people was mainly herder,accounting for 78.13％ (25/32);the onset time was mostly from May to September,accounting for 83.33％ (15/18).Conclusions Prevalence of human brucellosis in Gonghe County is still active in some local areas.We should strengthen health education and professional intervention in occupational populations to improve people's ability in prevention and control of the disease.

Objective:The epidemic regularity and trend of human brucellosis in Qinghai Province were analyzed to provide basis for formulating more perfect prevention and control measures.Methods:Using descriptive epidemiological methods, the data of brucellosis reported cases in Qinghai Province from 2005 to 2018 (from the Infectious Disease Report Information Management System of the China Disease Prevention and Control Information System) were collected, and the three distributions (time, region and population distributions) of human brucellosis were statistically described and analyzed.Results:From 2005 to 2018, a total of 418 cases were reported in Qinghai Province, with a incidence of 0.05/100 000, the incidence of different years was statistically different (χ 2 = 11.63, P < 0.05). The incidence of brucellosis remained at a low level from 2005 to 2010, ranged from 0 to 0.05/100 000; and the incidence increased from 2011 to 2018, ranged from 0.27/100 000 to 2.06/100 000. There were reported cases of brucellosis throughout the year, and the onset time was mainly from April to June, accounting for 42.82% (179/418). The 418 cases were distributed in 20 counties (cities, districts), among which the top 5 incidence rates were Menyuan County (15.31%, 64/418), Henan County (13.40%, 56/418), Tianjun County (13.16%, 55/418), Xining City (12.44%, 52/418), and Haiyan County (7.66%, 32/418). In the population distribution, brucellosis cases in the age group from 40 to 49 years old were the most, accounting for 32.78% (137/418), the male to female ratio was 1.58 ∶ 1.00 (256∶162) . And the occupational distribution was dominated by herdsmen, accounting for 57.18% (239/418). Conclusions:The epidemic situation of brucellosis in Qinghai Province is on the rise. It should be strengthened the population monitoring and notification of outbreaks, and health education for all should be implemented.

Objective:To observe multiple locus variable-number tandem repeat analysis (MLVA) typing of Brucella isolated from Himalayan marmot in Qinghai-Tibet Plateau of Qinghai Province, and to explore the relationship between the strains and strains previous isolated from Qinghai Province. Methods:Blood samples of Himalayan marmot were collected in Qinghai-Tibet Plateau of Qinghai Province from March 2019 to October 2020. Pathogens were isolated and cultured from Brucella antibody positive samples identified by using the rose bengal test (RBT). Conventional biological methods and molecular biological methods (BCSP31-PCR and AMOS-PCR) were used for strain identification. At the same time, MLVA method was used to genotype the isolated strains, and cluster analysis was used to analyze the genetic relationships between the strains based on the genotype of 70 Brucella isolated from different hosts in Qinghai Province. Results:A total of 1 466 blood samples of Himalayan marmot were collected from Qinghai-Tibet Plateau. Two strains of Brucella were isolated and cultured from 64 RBT-positive samples, named QH2013054 and QH2013062, respectively. They were identified as Brucella ovis biotype Ⅲ by conventional and molecular biological methods. The MLVA genotyping results showed that QH2013054 and QH2013062 were different at the Bru16 locus, indicating different MLVA genotypes. Cluster analysis showed that strain QH2013054 had the same MLVA genotype as 7 strains, among which 6 strains were from 3 farmers and 3 sheep from the same family in Gonghe County, and 1 strain was from a farmer in Menyuan Hui Autonomous County. The strain QH2013062 had the same MLVA genotype as 4 strains, including 3 strains from 3 farmers in Menyuan Hui Autonomous County and 1 strain from a farmer in Tu Autonomous County of Huzhu. Conclusions:The strains of Brucella isolated from Himalayan marmot in Qinghai-Tibet Plateau of Qinghai Province have the same MLVA genotype as some strains of Brucella isolated from humans and sheep in Qinghai Province. It is speculated that the host humans, sheep and Himalayan marmot in Qinghai-Tibet Plateau may have a common source of infection.

Objective:To learn about the epidemic situation and trend of human brucellosis in Menyuan Hui Autonomous County (referred to as Menyuan County) of Qinghai Province, and to provide reference for formulating brucellosis prevention and control measures in Menyuan County.Methods:Data on human brucellosis in Menyuan County reported by the Infectious Disease Reporting Information Management System of China Disease Control and Prevention Information System from 2013 to 2020 were collected and analyzed by descriptive epidemiological analysis (three distribution).Results:A total of 186 cases of brucellosis were confirmed from 2013 to 2020, with an average annual incidence of 14.553/100 000. The annual incidence rate was increasing year by year(χ 2trend = 22.08, P = 0.002). The cases were distributed in 67 villages of 12 towns; cases were more common in the age group of 15-< 65 years old (96.24%, 179/186), and there were more men than women cases (sex ratio was 3.89∶1.00, 148/38). Conclusions:The incidence rate of brucellosis cases in Menyuan County is increasing year by year, and the scope of its impact is constantly expanding. A multi-sectoral joint prevention and control mechanism should be established to strengthen management, increase the prevention and control of brucellosis, and stop the spread of the epidemic to surrounding counties and cities.

Objective:To investigate the efficacy and safety of a simple three-step excision for combined reduction plastic surgery of labia minora clitoral hood complex.Methods:We selected the clinical data of patients with labia minora and clitoral prepuce hypertrophy treated in the Department of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, from May 2017 to November 2021. All the patients underwent composite labia minora and clitoral hood reduction using a simple three-step excision. In the first step, a longitudinal shuttle incision was designed to remove the redundant lateral prepuce of the clitoris; In the second step, the hypertrophic part of labia minora was removed by wedge resection; The third step was to trim the triangular flap formed by the excess skin at the junction of the labia minora and the clitoral hood, and then complete the incision suture. Patients were asked to return for examinations on days 1, 7, and 10 postoperatively. After that, the patients were followed up in the outpatient department or online, and the satisfaction questionnaires were conducted. The contents included appearance improvement, preoperative symptom improvement, postoperative discomfort symptoms, sexual life quality, partner’s evaluation of surgical effect, and patient’s evaluation of surgical impact.Results:A total of 295 patients were enrolled, with an average age of 29 years, ranged from 9 to 52 years old. Patients were divided into 4 categories according to the surgeries they received, including 21 patients (7.1%) with unilateral labia + unilateral prepuce hypertrophy, 12 patients (4.1%) with unilateral labia + bilateral prepuce hypertrophy, 50 patients (16.9%) with bilateral labia + unilateral prepuce and 212 patients (71.9%) with bilateral labia + bilateral prepuce hypertrophy. The incidence of complications was 3.4%(10/295), hematoma occurred in 8 cases, scar hyperplasia with obvious pain in 1 case, and blood supply disorder in 1 case. After the complementary treatments, all patients recovered well from surgeries. 219 questionnaires were obtained three months after surgeries, and 76 cases were lost to follow-up. The overall satisfaction rate of patients was 94.5%(207/219).Conclusions:The preoperative design of three-step resection is simple, which can safely and effectively carry out composite labia minora and clitoral hood reduction, with less complications, concealed incision, slight scar, and high satisfaction.

Objective:To investigate the efficacy and safety of a simple three-step excision for combined reduction plastic surgery of labia minora clitoral hood complex.Methods:We selected the clinical data of patients with labia minora and clitoral prepuce hypertrophy treated in the Department of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, from May 2017 to November 2021. All the patients underwent composite labia minora and clitoral hood reduction using a simple three-step excision. In the first step, a longitudinal shuttle incision was designed to remove the redundant lateral prepuce of the clitoris; In the second step, the hypertrophic part of labia minora was removed by wedge resection; The third step was to trim the triangular flap formed by the excess skin at the junction of the labia minora and the clitoral hood, and then complete the incision suture. Patients were asked to return for examinations on days 1, 7, and 10 postoperatively. After that, the patients were followed up in the outpatient department or online, and the satisfaction questionnaires were conducted. The contents included appearance improvement, preoperative symptom improvement, postoperative discomfort symptoms, sexual life quality, partner’s evaluation of surgical effect, and patient’s evaluation of surgical impact.Results:A total of 295 patients were enrolled, with an average age of 29 years, ranged from 9 to 52 years old. Patients were divided into 4 categories according to the surgeries they received, including 21 patients (7.1%) with unilateral labia + unilateral prepuce hypertrophy, 12 patients (4.1%) with unilateral labia + bilateral prepuce hypertrophy, 50 patients (16.9%) with bilateral labia + unilateral prepuce and 212 patients (71.9%) with bilateral labia + bilateral prepuce hypertrophy. The incidence of complications was 3.4%(10/295), hematoma occurred in 8 cases, scar hyperplasia with obvious pain in 1 case, and blood supply disorder in 1 case. After the complementary treatments, all patients recovered well from surgeries. 219 questionnaires were obtained three months after surgeries, and 76 cases were lost to follow-up. The overall satisfaction rate of patients was 94.5%(207/219).Conclusions:The preoperative design of three-step resection is simple, which can safely and effectively carry out composite labia minora and clitoral hood reduction, with less complications, concealed incision, slight scar, and high satisfaction.

Objective:To investigate the efficacy and safety of a simple three-step excision for combined reduction plastic surgery of labia minora clitoral hood complex.Methods:We selected the clinical data of patients with labia minora and clitoral prepuce hypertrophy treated in the Department of Plastic and Aesthetic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, from May 2017 to November 2021. All the patients underwent composite labia minora and clitoral hood reduction using a simple three-step excision. In the first step, a longitudinal shuttle incision was designed to remove the redundant lateral prepuce of the clitoris; In the second step, the hypertrophic part of labia minora was removed by wedge resection; The third step was to trim the triangular flap formed by the excess skin at the junction of the labia minora and the clitoral hood, and then complete the incision suture. Patients were asked to return for examinations on days 1, 7, and 10 postoperatively. After that, the patients were followed up in the outpatient department or online, and the satisfaction questionnaires were conducted. The contents included appearance improvement, preoperative symptom improvement, postoperative discomfort symptoms, sexual life quality, partner’s evaluation of surgical effect, and patient’s evaluation of surgical impact.Results:A total of 295 patients were enrolled, with an average age of 29 years, ranged from 9 to 52 years old. Patients were divided into 4 categories according to the surgeries they received, including 21 patients (7.1%) with unilateral labia + unilateral prepuce hypertrophy, 12 patients (4.1%) with unilateral labia + bilateral prepuce hypertrophy, 50 patients (16.9%) with bilateral labia + unilateral prepuce and 212 patients (71.9%) with bilateral labia + bilateral prepuce hypertrophy. The incidence of complications was 3.4%(10/295), hematoma occurred in 8 cases, scar hyperplasia with obvious pain in 1 case, and blood supply disorder in 1 case. After the complementary treatments, all patients recovered well from surgeries. 219 questionnaires were obtained three months after surgeries, and 76 cases were lost to follow-up. The overall satisfaction rate of patients was 94.5%(207/219).Conclusions:The preoperative design of three-step resection is simple, which can safely and effectively carry out composite labia minora and clitoral hood reduction, with less complications, concealed incision, slight scar, and high satisfaction.