Objective To observe the clinical curative effect of Bushen Tiaochong method on treating early unexplained recurrent spontaneous abortion (URSA). Methods 200 patients who conformed to the inclusion criteria were randomly and paralleled control study divided into two groups.The control group (routine tocolysis group,n =100 ),with 8 cases fell off. The experimental group (routine tocolysis group +traditional Chinese medicine group,n =100 ),with 10 cases fell off. Results After the treatment,the total efficiency of the control group was 70.65%,and the experimental group was 88.89%. The total efficiency of comparative difference was statistically significant (p<0.05 ). And compared the experimental group with the control group,the former group improved the level of human chorionic gonadotropin(HCG)in patients. Conclusion Bushen Tiaochong method with traditional Chinese medicine combined western medicine treatment can significantly improve the symptoms of URSA patients with threatened abortion. Compared with simple western medicine therapy,the former group has better curative effect on early unexplained recurrent spontaneous abortion.

EEGs performed for new-onset seizures show epileptiform discharge in approximately 18% to 56% of children and 12%to 50%of adults. EEG is the most commonly used means of neurological examination for epilepsy. Speciifc EEG abnormalities help characterize the seizure type and epilepsy syndrome, which allows more informed decisions regarding therapy and more accurate prediction of seizure control and ultimate remission. In certain cases, the EEG may detect more subtle seizures, including absence, myoclonic or partial seizures. In the therapy of epilepsy, the effect of different antiepileptic drugs on the inhibition of epileptiform discharges is different. Epileptiform discharges play a very important role in the prediction of recurrence and the decision to remove antiepileptic drugs.

Objective: To analyze the clinical value of 16-slice CT angiography techniques in cerebral artery lesions. Methods:Forty three cases in our hospital with suspected cerebrovascular disease who performed computed tomography angiography were retrospectively analyzed. Volumetric imaging, maximum intensity projection and multi-planar reconstruction technique for three-dimensional reconstruction process were used in post-processing. Results:There were 27 cases with no abnormal blood vessels, 6 cases with aneurysm, 1 with arteriovenous malformation, 8 with arterial stenosis and 1 with congenital abnormalities. The image quality analysis of cerebral vascular trunk and main branches was 100%performed in 43 cases. The location, shape, size, and the relationship with the surrounding blood vessels and skull of the aneurysm have been clearly shown, and the nidus, feeding arteries and draining veins have also been clearly shown. Conclusion:With the features of safe, fast, non-invasive and clarity in images, 16-slice CT angiography imaging technology can be used as the first choice of screening cerebral arterial disease.

Improving medical students' legal quality is not only the requirements of modern education and their self-improvement,but also the needs of reform on medical system in the new era and establishment of a harmonious relationship between doctors and patients.Medical universities should carefully integrate the existing law education resources and establish three staged teaching model (stages of basis law education,health law education and stage of comprehensive improvement) when being fronted with the education model which is unilateral,sporadic and lack of cohesion in order to improve the law quality of medical students.

Objective To investigate the induced expression of indoleamine 2,3-dioxygenase in epithelial cells of cultured human proximal renal tubule under the stimulation of interferon-?(IFN-?)in vitro.Methods The expression of IDOmRNA in epithelial cells of human proximal renal tubule under normal and different dosage or time of IFN-? stimulation were assayed by semi-quantitative RT-PCR,the expression of IDO protein under normal and 2000U/ml IFN-? stimulation for 24h were assayed by immunocytochemistry,and the activity of IDO under different dosage of IFN-? stimulation was assessed by the special kynurenine metabolizable ratio in culture medium detected by high performance liquid chromatography(HPLC).Results No expression of IDO mRNA or IDO was found in the epithelial cells of human proximal renal tubule under normal culture conditions.IDO mRNA expression increased significantly after IFN-? stimulation in a dose dependent manner compared with that of control group(P

Antigens, CD34 ; metabolism ; Hemangioendothelioma, Epithelioid ; metabolism ; pathology ; surgery ; Hepatectomy ; methods ; Humans ; Liver Neoplasms ; metabolism ; pathology ; surgery ; Male ; Middle Aged ; Vascular Endothelial Growth Factor A ; metabolism ; von Willebrand Factor ; metabolism

In order to have a better understanding of the species diversity of medical plants in Enshi, Hubei of China, extensive field investigations and specimen collections were conducted in Enshi and adjacent regions. Based on field observations of plants in their living habitats and comparative morphological studies on specimens in herbarium of Hubei minzu University and other available herbaria as well, three new records of medical plants in Hubei, Scutellaria yunnanensis, Alangium faberi var. heterophyllum, and Drymaria diandra, were reported in this paper.
China ; Plants, Medicinal ; Records as Topic

Objective To evaluate if the assay of platelet-associated immunoglobulin G (PAIgG) can improve the sensitivity of monoclonal antibody immobilization of platelet antigens assay (MAIPA) in immune thrombocytopenic purpura (ITP).Methods Anti-GP Ⅱb/Ⅲa and anti-GP Ⅰb/Ⅸ autoantibodies were detected by a modified MAIPA;PAIgG was detected by competitive ELISA.MAIPA and PAIgG were done simultaneously in 190 patients with thrombocytopenia,in which 132 were diagnosed as ITP according to the diagnostic criteria Kappa test was used to analyze the concordance between MAIPA and PAIgG.Results The sensitivities of PAIgG alone or in parallel with MAIPA to ITP were 32.6% and 37.2% higher than MAIPA alone respectively,while their specifieities were 51.7% and 56.9% lower respectively.The sensitivity,specificity,positive predictive value,negative predictive value of PAIgG in series with MAIPA were all not superior to MAIPA alone.The kappa values between MAIPA and PAIgG in ITP and non-immune thrombocytopenia were 0.129 and 0.012 respectively,which meant the concordance between them was poor.Conclusion The detection of PAIgG should not be used as screening before MAIPA in distinguishing immune from non-immune thrombocytopenia.

AIM:To investigate the protective effect of hyperoside (Hyp) on Mycoplasma pneumoniae (MP) pneumonia (MPP) mice and the underlying regulatory mechanism.METHODS:BALB/c mice (n=40) were randomly divided into control group, model group, low-dose (12.5 mg/kg) Hyp group and high-dose (50 mg/kg) Hyp group.The MPP model was established using MP standard strains (MPFH) through nasal dripping.The treatment started on the second day, and the follow-up experiments were performed after 3 d.The lung histopathology were detected by HE staining.The levels of C-reactive protein (CRP), alanine aminotransferase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH) and MB isoenzyme of creatine kinase (CK-MB) in the serum, and the levels of reactive oxygen species (ROS), superoxide dismutase (SOD) and glutathione (GSH) in the lung tissues were measured by automatic biochemical analyzer.ELISA and Western blot were used to detect interleukine-1 beta (IL-1β), IL-6, IL-18 and tumor necrosis factor-alpha (TNF-α) levels in the serum and lung tissues, respectively.The expression of NLRP3, ASC and caspase-1 at mRNA and protein levels was evaluated by RT-qPCR and Western blot.RESULTS:Compared with control group, the inflammation and alveolar interstitial widening were observed in the lung tissues of model mice, and the CRP, ALT, AST, LDH and CK-MB levels were significantly up-regulated (P<0.05).The ROS level was increased, while SOD and GSH levels were significantly decreased (P<0.05).The levels of IL-1β, IL-6, IL-18 and TNF-α were markedly up-regulated (P<0.05).The protein expression of NLRP3, ASC and caspase-1 was also obviously increased (P<0.05) in model group.Compared with model group, the symptoms above were significantly relieved in Hyp group, and the effect of high-dose Hyp was more apparent than that of the low-dose one.CONCLUSION:Hyperoside suppresses the injury in MPP mice, which may be related to the inhibition of oxidative stress and NLRP3 inflammasome activation.

Objective To investigate the expression of indoleamine 2,3-dioxygenase(IDO)in renal tissues of crescent nephritis and its correlation with cell proliferation and pathologic tubulointerstitial lesion.Methods Immunohistochemistry was used to detect IDO expression in renal tissues of crescent nephritis and normal renal tissues.The correlation between IDO expression and the number of proliferating cell nuclear antigen(PCNA)positive cells or pathologic tubulointerstitial lesion were analyzed.Results IDO expression was found in the renal tubular epithelial cells of crescent nephritis,while that in the normal renal tissues was negative by immunohistochemical staining.The expression of IDO protein in tubular epithelial cells showed a significantly negative correlation with the number of PCNA positive cells or tubulointerstitial lesion of crescent nephritis.Conclusion IDO may participate in the pathogenesis of crescent nephritis by inhibiting the cell proliferation.