Gene ADAM10 (a disintegrin and metalloproteinase 10) is a member of ADAMs gene family. The corresponding membrane protein contains a disintegrin domain and a metalloproteinase domain. It is involved in various biological events such as cell adhesion, cell fusion, cell migration, and membrane protein shedding and proteolysis. ADAM10 protein is overexpressed in many tumors, which interacts with adhesion molecules and critical signaling molecules associated with cancer development and tumor progression, and promotes tumor invasion and metastasis. Regulating the expression of ADAM 10 can inhibit tumor invasion and metastasis , which may become a new strategy for anticancer therapy.

This article concerned the idea and working theory of YL-Ⅱ computer's hardware circuit for diet therapy along with the idea of system software, application software and some flowcharts. Thereby, the nutrition specialist system was implemented in a system centred in one slice including.A.The composition of YL-Ⅱ computer for diet therapy.B.The design of YL-Ⅱ nutrition medical treatment computer's hardware circuits for diet therapy. C.The design of YL-Ⅱ computer's software for diet therapy.D.The design of YL-Ⅱ computer's application software for diet therapy. There were 4 main functions of the YL-Ⅱcomputer used in clinic;A.Offering a variety of diet menu; 9 kinds and 84 types of diet menu were stored in the computer, and could be used according to the change of patient's content and the actual intake of the patient.B.Nutritional assessment; To give nutritional assessment with patient's height, weight, skinfold thickness and biochemical parameters etc.C.Nuturitional advisory; the nutritional guide for individual or group.D.Nutritional inquiry.The YL-Ⅱ nutrition medical treatment computer had been used in the clinic of 50 hospitals, all the staffs acknowledged its effectiveness, usefulness, accuracy and time saving, and raising working efficiency 25 times.

Infection is a major complication in lupus nephritis patients,and also remains a major cause of morbidity and mortality in LN.The most common infected sites are respiratory tract,skin soft tissue,urinary system,et al.Immunological abnormity,use of glucocorticoid and immunosuppressive agents,renal insufficiency and hypoproteinemia are involved in the susceptibility of LN patients' infections.A wide variety of infectious pathogens are recognized in LN and bacterial infection is the most.Virus,fungi and tubercle bacillus also can be identified.The clinical manifestations of the infections are atypical,including fever,cough,headache,twitch,abnormity of spirit and action,et al and often confused with the multiplicity clinical manifestations of SLE activity.It is necessary to carry out clinical observation and pathogeny examination for diagnosis.All patients must receive effective anti-infective therapy in time,and adjust their glucocorticoid and immunosuppressive dosage.In order to avoid infection,it is necessary to strictly control the indication and dosage of glucocorticoid and immunosuppressers.

A total of 122 patients with acute left ventricular failure from January 2011 to December 2013 at our hospital were recruited and divided randomly into control group (n =62) and observation group (n =60).Control group received conventional therapy and Bi-level positive airway pressure (Bi-PAP) was added along with conventional therapy for observation group.And 12 and 8 patients died in control and observation groups respectively within 24 h after treatment and there was no statistical difference.The BNP levels of the control group pre-treatment and at 1 h and 24 h post-treatment were (1 040 ± 315),(995 ± 245) and (570 ± 164) ng/L.The BNP levels of observation group pre-treatment and at 1 h and 24 h posttreatment were (1 080 ±209),(995 ±245) and (490 ± 138) ng/L.BNP of observation group at 1 h and 24 h post-treatment decreased significantly compared with that pre-treatment and control group (P ＜0.01).Only at 24 h post-treatment,the BNP level of control group decreased significantly compared with that pretreatment (P ＜ 0.01).

Objective:To examine the reliability and validity of the Chinese version of the eating beliefs questionnaire (EBQ-18) with Chinese subjects.Methods:Totally 230 university students were recruited for the preliminary survey of the EBQ-18(224 valid questionnaires were recovered). The formal EBQ-18 was set through item analysis and exploratory factor analysis.Then, 690 university students were selected to complete the formal EBQ-18 for the confirmatory factor analysis and the internal consistency analysis(674 valid questionnaires were recovered). Adult eating behavior evaluation questionnaire(AEBEQ)were selected as the criterion measurement which to examine the association between eating beliefs and eating behavior.Test-retest reliability were examined by 260 participants randomly selected after two weeks.Data analysis was conducted by using SPSS 23.0 and AMOS 22.0 software.Results:The Chinese version of the EBQ-18 with 18 items had 3 dimensions, which were negative beliefs, positive beliefs and permissive beliefs.Confirmatory factor analysis showed that the Chinese version of the EBQ-18 had good structure validity(χ 2=333.82, df=132, RMSEA=0.08, NFI=0.91, CFI=0.92, GFI=0.90, PGFI=0.66). Cronbach α coefficient and the test-retest reliability were 0.91 and 0.87 for the total scale.The internal consistency coefficients were 0.87, 0.91, 0.86, and the test-retest reliability were 0.89, 0.84, 0.90 for the 3 factors. Conclusion:It suggests that the Chinese version of EBQ-18 is a reliable and valid assessment and can be used as an effective and reliable tool for assessing eating beliefs of university students in China.

Objectives To study the effect of preoperative platelet transfusion for splenectomy and devascularization in the prevention of intraoperative and postoperative bleeding.Methods The 230 patients with cirrhosis and portal hypertension who received splenectomy and periesophagogastric davascularization were divided into strata A,B and C according to the platelet counts.Stratum A patients had a platelet count of less than 30× 10/L,B between 30× 10/L and 50× 109/L,and C more than 50 × 109/L.The patients in each stratum were then randomly divided into a preoperative transfusion group (T group) and a non-transfusion group (NT group).The amounts of intraoperative bleeding,postoperative drainage in 48 hours after operation,rates of postoperative bleeding,and general medical conditions were compared.Results A comparison in stratum A showed lower amounts of intraoperative bleeding and 48 hour postoperative drainage,and a lower rate of bleeding in the T group (P＜0.05).There were no significant differences between the T and the NT groups in strata B and C (P＞0.05).Conclusions For patients with a platelet count lower than 30 × 109/L,preoperative platelet transfusion significantly reduced bleeding suggesting that preoperative platelet transfusion for splenectomy and periesophagogastric devascularization should be a routine.For those patients whose platelet count was above 30 × 109/L,platelet transfusion is not recommended.

Humans ; Organophosphorus Compounds ; analysis ; pharmacokinetics

In order to study the inherent difference among terpenes producing yeasts from the point of metabolomics, we selected taxadiene producing yeasts as the model system. The changes of cellular metabolites during fermentation log phase of artificial functional yeasts were determined using metabolomics methods. The results represented that compared to W303-1A as a blank control, the metabolites in glycolysis, tricarboxylic acid cycle (TCA) cycle and several amino acids were influenced. And due to the changes of metabolites, the growth of cells was inhibited to a certain extent. Among the metabolites identified, citric acid content in taxadiene producing yeasts changed the most, the decreasing amplitude reached 90% or more. Therefore, citric acid can be a marker metabolite for the future study of artificial functional yeasts. The metabolomics analysis of taxadiene producing yeasts can provide more information in further studies on optimization of terpenes production in heterologous chassis.
Alkenes ; metabolism ; Amino Acids ; metabolism ; Citric Acid ; analysis ; Citric Acid Cycle ; Diterpenes ; metabolism ; Fermentation ; Glycolysis ; Metabolome ; Metabolomics ; Yeasts ; metabolism

This article elaborated on the necessity and importance of reform of medical education administration system in comprehensive university in promoting comprehensive development of medical education,from the point of merging of universities.It also emphasized the long-term nature and complexity of the reform process.In the meantime,it introduced the reform measures and effects of the reform in the author's university.

OBJECTIVE: To study the effects of total saponins of Panax japonicus (TSPJ) on human leukemic HL-60 cells. METHODS: Human leukemic HL-60 cells were cultured in vitro. The cancer cell vigor was detected by using cell counting kit-8. Nitroblue tetrazolium (NBT) was used for measuring cell reduction. The cell cycle and the expression of differentiation antigen CD11b were detected by flow cytometry. RESULTS: Compared with the negative control group, TSPJ in different concentrations could decrease the vigor of HL-60 cells and the number of cells in S phase and up-regulate the CD11b expression, while the numbers of NBT positive cells and cells in G(0)/G(1) phase in the different concentrations of TSPJ-treated groups were increased. CONCLUSION: TSPJ can inhibit the HL-60 cell growth in vitro. Its mechanism may be related to inhibiting proliferation and inducing cell differentiation and cycle arrest.