Gene ADAM10 (a disintegrin and metalloproteinase 10) is a member of ADAMs gene family. The corresponding membrane protein contains a disintegrin domain and a metalloproteinase domain. It is involved in various biological events such as cell adhesion, cell fusion, cell migration, and membrane protein shedding and proteolysis. ADAM10 protein is overexpressed in many tumors, which interacts with adhesion molecules and critical signaling molecules associated with cancer development and tumor progression, and promotes tumor invasion and metastasis. Regulating the expression of ADAM 10 can inhibit tumor invasion and metastasis , which may become a new strategy for anticancer therapy.

This article concerned the idea and working theory of YL-Ⅱ computer's hardware circuit for diet therapy along with the idea of system software, application software and some flowcharts. Thereby, the nutrition specialist system was implemented in a system centred in one slice including.A.The composition of YL-Ⅱ computer for diet therapy.B.The design of YL-Ⅱ nutrition medical treatment computer's hardware circuits for diet therapy. C.The design of YL-Ⅱ computer's software for diet therapy.D.The design of YL-Ⅱ computer's application software for diet therapy. There were 4 main functions of the YL-Ⅱcomputer used in clinic;A.Offering a variety of diet menu; 9 kinds and 84 types of diet menu were stored in the computer, and could be used according to the change of patient's content and the actual intake of the patient.B.Nutritional assessment; To give nutritional assessment with patient's height, weight, skinfold thickness and biochemical parameters etc.C.Nuturitional advisory; the nutritional guide for individual or group.D.Nutritional inquiry.The YL-Ⅱ nutrition medical treatment computer had been used in the clinic of 50 hospitals, all the staffs acknowledged its effectiveness, usefulness, accuracy and time saving, and raising working efficiency 25 times.

Objective:To examine the reliability and validity of the Chinese version of the eating beliefs questionnaire (EBQ-18) with Chinese subjects.Methods:Totally 230 university students were recruited for the preliminary survey of the EBQ-18(224 valid questionnaires were recovered). The formal EBQ-18 was set through item analysis and exploratory factor analysis.Then, 690 university students were selected to complete the formal EBQ-18 for the confirmatory factor analysis and the internal consistency analysis(674 valid questionnaires were recovered). Adult eating behavior evaluation questionnaire(AEBEQ)were selected as the criterion measurement which to examine the association between eating beliefs and eating behavior.Test-retest reliability were examined by 260 participants randomly selected after two weeks.Data analysis was conducted by using SPSS 23.0 and AMOS 22.0 software.Results:The Chinese version of the EBQ-18 with 18 items had 3 dimensions, which were negative beliefs, positive beliefs and permissive beliefs.Confirmatory factor analysis showed that the Chinese version of the EBQ-18 had good structure validity(χ 2=333.82, df=132, RMSEA=0.08, NFI=0.91, CFI=0.92, GFI=0.90, PGFI=0.66). Cronbach α coefficient and the test-retest reliability were 0.91 and 0.87 for the total scale.The internal consistency coefficients were 0.87, 0.91, 0.86, and the test-retest reliability were 0.89, 0.84, 0.90 for the 3 factors. Conclusion:It suggests that the Chinese version of EBQ-18 is a reliable and valid assessment and can be used as an effective and reliable tool for assessing eating beliefs of university students in China.

A total of 122 patients with acute left ventricular failure from January 2011 to December 2013 at our hospital were recruited and divided randomly into control group (n =62) and observation group (n =60).Control group received conventional therapy and Bi-level positive airway pressure (Bi-PAP) was added along with conventional therapy for observation group.And 12 and 8 patients died in control and observation groups respectively within 24 h after treatment and there was no statistical difference.The BNP levels of the control group pre-treatment and at 1 h and 24 h post-treatment were (1 040 ± 315),(995 ± 245) and (570 ± 164) ng/L.The BNP levels of observation group pre-treatment and at 1 h and 24 h posttreatment were (1 080 ±209),(995 ±245) and (490 ± 138) ng/L.BNP of observation group at 1 h and 24 h post-treatment decreased significantly compared with that pre-treatment and control group (P ＜0.01).Only at 24 h post-treatment,the BNP level of control group decreased significantly compared with that pretreatment (P ＜ 0.01).

Infection is a major complication in lupus nephritis patients,and also remains a major cause of morbidity and mortality in LN.The most common infected sites are respiratory tract,skin soft tissue,urinary system,et al.Immunological abnormity,use of glucocorticoid and immunosuppressive agents,renal insufficiency and hypoproteinemia are involved in the susceptibility of LN patients' infections.A wide variety of infectious pathogens are recognized in LN and bacterial infection is the most.Virus,fungi and tubercle bacillus also can be identified.The clinical manifestations of the infections are atypical,including fever,cough,headache,twitch,abnormity of spirit and action,et al and often confused with the multiplicity clinical manifestations of SLE activity.It is necessary to carry out clinical observation and pathogeny examination for diagnosis.All patients must receive effective anti-infective therapy in time,and adjust their glucocorticoid and immunosuppressive dosage.In order to avoid infection,it is necessary to strictly control the indication and dosage of glucocorticoid and immunosuppressers.

Humans ; Organophosphorus Compounds ; analysis ; pharmacokinetics

Objectives To study the effect of preoperative platelet transfusion for splenectomy and devascularization in the prevention of intraoperative and postoperative bleeding.Methods The 230 patients with cirrhosis and portal hypertension who received splenectomy and periesophagogastric davascularization were divided into strata A,B and C according to the platelet counts.Stratum A patients had a platelet count of less than 30× 10/L,B between 30× 10/L and 50× 109/L,and C more than 50 × 109/L.The patients in each stratum were then randomly divided into a preoperative transfusion group (T group) and a non-transfusion group (NT group).The amounts of intraoperative bleeding,postoperative drainage in 48 hours after operation,rates of postoperative bleeding,and general medical conditions were compared.Results A comparison in stratum A showed lower amounts of intraoperative bleeding and 48 hour postoperative drainage,and a lower rate of bleeding in the T group (P＜0.05).There were no significant differences between the T and the NT groups in strata B and C (P＞0.05).Conclusions For patients with a platelet count lower than 30 × 109/L,preoperative platelet transfusion significantly reduced bleeding suggesting that preoperative platelet transfusion for splenectomy and periesophagogastric devascularization should be a routine.For those patients whose platelet count was above 30 × 109/L,platelet transfusion is not recommended.

The complete mitochondrial genome sequence of Phlaeoba albonema Zheng Was determined by using L-PCR and conserved primers walking sequencing.The obtained genome sequence is 15657 bp in size.containing 13 protein-coding genes,2 ribosomal RNA and 22 transfer RNA genes.All the 37 genes are conserved in the same orientations as observed in Locusta migratoria.11202 bp of the mtDNA are coding for proteins,1486 bp for tRNAs,1312 bp for rRNA large subunit(1rRNA),and 844 bp for rRNA small subunit(srRNA).The A+T-rich region is 728 bp in size.The genes overlapping sequences are 41 bp in total and are spreading over 9 locations(1-8 bp at each site).A total of 126 bp intergenic spacer sequences are scattered in 21 regions at the size of 1 to 20 bp,where the largest 20 bp region iS located between the tRNALys and ATP8 genes.The predicted secondary structures of both srRNA and lrRNA were compared with that of Ruspolia dubia,and the patterns of base pairs in tRNA anticodon stem and A/T,C/G bias of protein-coding genes in different strands were discussed.

AIM　To prepare naftopidil bioadhesive sustained-release capsule and study their pharmacokinetics and relative bioavailability in the dog. METHODS　Bioadhesive polymers such as hydroxypropyl methylcellulse (HPMC) and Carbopol 934 (CP 934) were used in capsule prescriptions. Naftopidil capsule and two formulations of bioadhesive sustained-release capsules (I and II) were given to five healthy male dogs in a cross-over test. The naftopidil concentrations in plasma were determined by a newly developed HPLC method and the pharmacokinetic parameters as well as the relative bioavailability were measured. RESULTS　The C0→∞, Cmax and Tmax of naftopidil capsule was (3728±573) h*ng*mL-1, (697±94) ng*mL-1 and (1.2±0.5) h. These parameters of bioadhesive sustained-release capsule I and II, respectively, were (5518±391) h*ng*mL-1 and (5636±427) h*ng*mL-1; (468±61) ng*mL-1 and (512±72) ng*mL-1; both (4.0±0.7) h. Results from statistics showed that there were significant difference between bioadhesive formulations and the non-bioadhesive one in C0→∞, Cmax and Tmax. The bioadhesive formulations and the non-bioadhesive one were not bioequivalent, the relative bioavailability of the two bioadhesive sustained-release capsules were respectively 150%±14% and 154%±23% when compared with the non-bioadhesive capsule. CONCLUSION　It is much improving bioavailability of naftopidil by using bioadhesion.

Objective:Hepatocyte growth factor(HGF) speculated to be an endothelial-specific growth factor which protects or repaire the vascular endothelial cells. To test the serum HGF level might be elevated in response to hypertension-induced endothelial cell damage, we measured serum HGF concentrations in normotensive and hypertensive subjects without liver, kidney and other complications.Methods:Eighteen male hypertensive patients and thirteen male normotensive subjects were recruited. All antihypertensive agents were stopped for 2 weeks before the study. The serum HGF concentrations were measured by a specific enzyme-linked immunosorbent assay (ELISA). Results:Serum HGF concentrations were 0.32±0.13 ng/ml in normotensive subjects and 0.37±0.27 ng/ml in hypertensive patients. No significant difference between the two groups(P>0.05) was found. Systolic, mean and diastolic blood pressure did not show any correlation with serum HGF concentrations.Conclusions:This study showed that serum HGF levels did not be increased in male patients with mild to moderate hypertension. Although local HGF is produced rapidly because of the damage of endothelial cells by high blood pressure, the circulating level of HGF did not represent the local changes of HGF production.