Objective To present the CT and MRI findings of the fibrous dysplasia(FD) of the spine.Methods CT and MRI findings were retrospectively evaluated in 19 cases of FD of spine,all of which were confirmed pathologically.Nineteen patients underwent CT plain scanning(19/19) and 5 had enhanced CT scanning(5/19).Patients were also examined by MRI plain scan(11/11) or MRI enhanced scan (6/11).The location of tumors,the type of bone destruction,the boundary of lesions,internal intensity or signal,the enhancement pattern of lesions,and presence of compression fractures,spinal deformity were observed.Result Nine cases had monostotic FD,while 10 had polyostotic FD.In all the 49 lesions of 19 cases,13 lesions were located in the cervical vertebrae,23 lesions in the thoracic vertebrae,11 lesions in lumbar vertebrae,and 2 lesions in sacral vertebrae.Thirty-three lesions involved both vertebral body and appendix.Pure osteolysis were found in 26 lesions on CT examinations.Peripheral osteosclerosis rims (41/49) and expansive lesions(32/49) were seen.Residual bone crest(28/49) and ground-glass opacity(23/49)were noted.Different degrees of vertebral compression were found in 19 lesions.Five patients had spinal deformity.On T1WI,14 lesions showed intermediate or low signal,and 10 lesions presented as heterogeneous signal.On T2WI,6 lesions had low signal intensity,4 lesions were noted as hyperintensive,and 14 lesions presented as heterogeneous signal.Multiple fluid-fluid levels were found in 1 lesion.Low signal rims were seen in 14 lesions.Twenty lesions of 11 patients had significant enhancement.Conclusion Expansive pattern,ground-glass opacity,peripheral osteosclerosis rims and significant enhancement were helpful findings for the diagnosis of spinal FD.

Objective To isolate and culture meshenchymal stem cells from murine fetal liver.Methods flMSCs from mouse fetuses were isolated by adhering to plastic surface.Growth kinetics was determined by growth curve.Cell cycle and phenotype were analyzed by FACSan flow cytometry.Differentiation of adhering cells was induced and identified.Results Homogenous fibroblast-like cells were predominated in culture.The counting of flMSCs increased 2 fold after 24 hours and 83.76%?2.88% of flMSCs were in G0/G1 phases.flMSCs were CD44,CD29 positive but negative for the markers of hematopoietic cells such as CD45,CD11b.flMSCs were able to differentiate along adipogenic,chondrogenic and osteogenic pathways even after being passaged several times.ConclusionflMSCs can be isolated by their plastic-attachable property and can expand without losing their multiple differentiation potential in vitro.flMSCs may offer an appropriate cell source for stem cell therapy.

Objective To investigate various sex hormones and the their relation in patients with polycystic ovary syndrome (PCOS).Methods 40 patients with PCOS were matched with 40 age matched healthy women,study group was divided into the obese group and non-obese groups based on body mass index,insulin resistance and non-insulin-resistance based on insulin sensitivity index. The level of follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), estradiol (E2), testosterone (T), androstene dione (A4), sulfal-dehydroepiandrosterone (DHEAS), sex hormone-binding globulin (SHBG), fasting glucose(FG), fasting insulin (FIN) was measured in both group. Homeostasis model assessment insulin resistance (HOMA-IR), insulin sensitivity index (ISI) and free androgen index (FAI) were caulculated. Results ①In the PCOS group,LH, LH/FSH, T, A4, FAI, FIN and Homa-IR were significantly higher compared to the control,while FSH, SHBG, ISI were significantly lower (P<0.05),PRL, FG, E2, DHEAS level did not show difference (P>0.05). ②There were significant differences in T, FAI, A4, but not in DHEAS between the PCOS group and the control one. ③There were significant differences between the hirsute group and the non-hirsute group in T, FAI, A4 (P<0.05). ④In the obese PCOS group compared to the non-obese PCOS group, T, A4, DHEAS were not significantly different, but FAI and Homa-IR were significantly higher, SHBG, ISI, LH/FSH were significantly lower. ⑤The quantity of insulin resistance in the increased T group was significantly higher compared to the common T group. ⑥In the insulin resistance group compared to the non insulin resistance group, there were not significant differences of A4、DHEAS,T and FAI was significantly higher.⑦In PCOS group,there were significant positive correlation between FAI useful parameter compared to T, A4, DHEAS for the diagnosis of PCOS. Obese PCOS women have more severe and BMI,FAI and Homa-IR;BMI and LH/FSH were significant inverse correlation. Conclusions FAI is more endocrine secretion and metabolic disturbance than non PCOS women. Hyperandrogenism and insulin resistance are consanguineous correlation.

ObjectiveIn order to confirm the relationship of pathogenic factors and clinical manifestation of cerebral palsy. Methods185 children were divided into the prematurity group(91 cases) and maturity group(94 cases). The μ-test was applied to analyze the incidence of clinical manifestation of 185 children with cerebral palsy for different pathogenic factors. ResultsThe sever symptoms occurred more frequently in prematurity group than in maturity group. ConclusionThe earlier the careful follow-up for children with prematurity was performed, the earlier the diagnosis and treatment of cerebral palsy were achiened.

Objective To investigate the effects of controlled hypotension induced with sodium nitroprusside (SNP) or nicardipine on hemodynamics during hip operation and postoperative hepatic and renal function.Methods Twenty ASA Ⅰ or Ⅱ patients aged 20-70 yrs weighing 50-80 kg scheduled for total hip replacement or open reduction and internal fixation of fracture of acetabulum were randomized to receive either SNP (group Ⅰ ) or nicardipine (group Ⅱ ) for induced hypotension during operation. Each group included 10 patients. Radial artery and right internal jugular vein were cannulated for BP and CVP monitoring. The probe of Hemo-Sonic ?100 esophageal supersonic hemodynamic monitor (Arrow U.S.A) was placed in the esophagus and fixed when best signal was obtained. ECG, hemodynamics including MAP, HR, CVP, CO and other parameters, SpO2 and PetCO2 were continuously monitored. Anesthesia was induced with propofol 2 mg?kg-1 , fentanyl 2-3 ?g? kg-1 and vecuronium 0.1 mg?kg-1 and maintained with 1.5% isoflurane and continuous infusion of propofol ( at 4-6 mg?kg-1 ?h-1 ) and intermittent i.v. boluses of vecuronium after tracheal intubation. MAP was reduced to 55-65 mm Hg with SNP (at a rate of 0.5-8.0 ?g?kg-1?min-1 ) or nicardipine (at 1.0-8.0 ?g?kg-1 ?min-1 ) . Intraoperative blood loss and blood transfusion and postoperative drainage from the wound were measured and recorded.Hemodynamic parameters were recorded before hypotension and 15, 30 and 60 min after start of induced hypotension and 15 and 30 min after recovery from hypotension. Hepatic and renal functions were measured before and after operation using total bilirubin, AST, ALT, BUN and creatinine. Results The two groups were comparable with respect to sex ratio (M/F) , age, height, body weight, duration of induced hypotension and operation. MAP and systemic vascular resistance (SVR) were significantly reduced and tachycardia developed during hypotension in both groups. The left ventricular ejection time was significantly prolonged and aortic blood flow (ABF) and cardiac output (CO) were significantly increased and CVP was lowered during hypotension as compared to the baseline values in SNP group. BUN was significantly reduced after operation ( P

Objective To evaluate the effect of reference mAs on radiation dose and image quality characteristics of chest CT scanned with CARE kV technique compared with CARE Dose4D.Methods According to the date of examination,158 consecutive patients were selected in our department and randomly divided into 5 groups:CARE Dose4D group (30 cases,group 1); using CARE kV technology,reference mAs were 110 (32 cases),90 (31 cases),70 (33 cases),50 (32 cases),which were recorded asthe group from 2 to 5,respectively.Volume CT dose index (CTDIvol),dose length product (DLP) and the effective dose (ED) were analyzed.CT value and objective image noise were measured in the lungparenchyma and descending aorta.Two radiologists assessed the images for subjective noise,artefacts and diagnostic acceptability.The radiation dose,CT value and objective noise were compared with the analysis of variance,and the difference between two groups was compared with SNK test; the rank sum tests (Kruskal-Wallis) were used in subjective image quality score,and difference between two groups was compared with K-W test.Results The CTDIvol,DLP,ED values in five groups were (7.7±1.7),(7.7±2.0),(5.5±1.4),(4.2±1.5),(2.8±1.2)mGy,(290.7±67.4),(290.1±85.2),(194.2±52.1),(150.7±63.8),(96.5±38.9) mGy· cm,(4.1±0.9),(4.1±1.2),(2.7±0.7),(2.1 ± 0.9),(1.3±0.5) mSv,respectively.There were significant difference among the 5 groups(F=59.305,57.76,57.76,P＜0.01).With the decrease of the reference mAs,the radiation dose decreased significantly.There were no significant difference among the 5 groups on the average CT values of lung parenchyma and descending aorta(F=0.353,0.102,P＞0.05).The objective noise of 5 groups in pulmonary parenchyma and the descending aorta were (48.7 ± 9.1),(49.2 ± 10.0),(55.5± 11.2),(56.5± 8.3),(63.7 ± 13.2) HU; (9.2± 1.6),(9.5 ± 2.1),(10.7 ± 2.6),(11.6 ± 2.7),(13.6 ± 2.6) HU respectively,and the difference was significant(F=10.774,17.157,P＜0.01).Level assessment onsubjective noise of five groups by radiological expertsalso showed the significant difference (U=33.967,P＜0.01),butthe evaluation on motion,streak,blotchy pixellatedartefacts and diagnostic acceptability had no statistical difference (U=8.847,2.054,6.186,5.296,P＞0.05).Conclusion Comparedwith CARE Dose4D,the use of CARE kV technique of suitable reference mAs for unenhanced chest CT can reduce radiation dose significantly with diagnostically acceptable image quality.

Objective To explore the MRI diagnostic value for pernicious placental abnormalities.Methods MRI findings of 1 5 patients with pernicious placenta previa were retrospectively analyzed after cesarean section.Results Among all cases,total placenta previa was,1 1 cases and partial placenta previa was 4 cases.The placenta located in the anterior wall in 8 cases,posterior wall in 4 cases,lateral wall in 3 cases.4 cases were diagnosed as normal placenta,6 cases as adhesion,4 cases as implanted,and 1 case as penetrating.These cases had certain special MR features.Conclusion MRI is helpful in diagnosing the location and type of the perni-cious placenta,and showing whether complicated with previa implantation and penetrating.

Objective To investigate the effect of vitamin D on pulmonary function in patients with chronic obstructive pulmona-ry disease,and to explore its possible mechanism.Methods Patients with chronic obstructive pulmonary disease admitted in the hospital were randomly divided into 2 groups,one group of patients were given regular COPD treatment (control group,30 cases), the other group of patients were given vitamin D on the basis of conventional therapy(experimental group,30 cases).Pulmonary function test and C reaction protein (CRP)determination in the 2 groups were performed,the contents of PaO2 ,PaCO2 were also determined.Results Compared with the control group,lung function improvement in experimental group were more obvious,and the the number of acute attack significantly reduced(P <0.05).The concentration of CRP in experimental group was significantly lower after treatment than before treatment(P <0.05),while the concentration of CRP in control group didn′t change significantly (P >0.05).Conclusion Vitamin D contributes to the improvement of pulmonary function in patients with COPD,and its mecha-nism might relate to the reduction of inflammatory reaction.

BACKGROUND:Studies have found that β-casomorphin 7 can lessen oxidation of renal tubular epithelial cels and lower blood sugar in a rat model of diabetes. There is a few studies concerning the effect of β-casomorphin-7 on diabetic nephropathy. OBJECTIVE:To explore the effects of β-casomorphin-7 on renal injury in a rat model of diabetic nephropathy. METHODS:The 30 rats were equaly and randomly divided into control group, model group andβ-casomorphin-7 group. Twenty rat models of diabetes were established. In the model and β-casomorphin-7 groups, rats were intraperitonealy injected with streptozotocin (60 mg/kg). In the control group, rats were intraperitonealy injectedwith an equal volume of citric acid. Rats in the β-casomorphin-7 group were intragastricaly administeredβ-casomorphin-7 for 30 days. Rats in the model and control groups were given an equal volume of saline by intragastric administration. RESULTS AND CONCLUSION: (1) Compared with the control group, insulin levels decreased, glucagon, blood glucose, urine glucose, urine protein, serum creatinine, serum urea nitrogen levels, mRNA and protein expression levels of type I colagen and type IV colagen in kidney increased in the model group. (2) Compared with the model group, above indexes were apparently recovered in the β-casomorphin-7 group. These findings indicate that β-casomorphin-7 has a protective effect on renal injury in diabetic nephropathy rats.

BACKGROUND: Safflower yel ow is known to treat diabetic nephropathy, can protect kidney function, reduce lesions, delay or prevent the development of diabetic nephropathy.
OBJECTIVE: To further verify the effects of safflower yel ow on the kidney in rats with diabetic nephropathy and the impact of safflower yel ow on the expression of renal cel receptor of angiotensin II1.
METHODS: Thirty rats were equally and randomly divided into three groups: normal control group, model group and experimental group. In the experimental group and model group, rat models of diabetic nephropathy were established. At 1 week after model establishment, rats were daily intraperitoneally given safflower yellow injection 27.8 mg/kg, once a day. In the model and control groups, rats were daily intraperitoneally given an equal volume of saline. All rats were administered for 23 weeks. The kidney-related biochemical indicators and pathological changes in kidney tissue and the expression of angiotensin II1 type receptor on kidney tissue of rats were observed.
RESULTS AND CONCLUSION: (1) Hypertrophy index of experimental group and model group was significantly higher than that of control group (P < 0.05). (2) 24-hour proteinuria, glycosylated hemoglobin, total cholesterol, triglycerides, creatinine and urea nitrogen of model group were higher than that of control group (P < 0.05). 24-hour proteinuria, glycated hemoglobin, total cholesterol and urea nitrogen in experimental group were higher than that of control group (P < 0.05). 24-hour proteinuria, total cholesterol, triglycerides, creatinine and urea nitrogen in the experimental group were lower than that of model group (P < 0.05). (3) Glomerular hypertrophy, thickening of capillary basement membrane, cell proliferation, and mesangial widening were detected in the model group. Abnormal changes in the kidney structure were found in the control group. The extent of damage of kidney of histopathology in the experimental group was between the control group and the model group. (4) The expression level of angiotensin receptor II1 of experimental group and model group was higher than that of control group (P < 0.05). The expression level of angiotensin receptor II1 of experimental group was lower than that of model group (P < 0.05). These results indicated that safflower yellow has a protective effect on diabetic nephropathy; the mechanism of action may be associated with blocking kidney partial renin-angiotensin system.