Objective To present the CT and MRI findings of the fibrous dysplasia(FD) of the spine.Methods CT and MRI findings were retrospectively evaluated in 19 cases of FD of spine,all of which were confirmed pathologically.Nineteen patients underwent CT plain scanning(19/19) and 5 had enhanced CT scanning(5/19).Patients were also examined by MRI plain scan(11/11) or MRI enhanced scan (6/11).The location of tumors,the type of bone destruction,the boundary of lesions,internal intensity or signal,the enhancement pattern of lesions,and presence of compression fractures,spinal deformity were observed.Result Nine cases had monostotic FD,while 10 had polyostotic FD.In all the 49 lesions of 19 cases,13 lesions were located in the cervical vertebrae,23 lesions in the thoracic vertebrae,11 lesions in lumbar vertebrae,and 2 lesions in sacral vertebrae.Thirty-three lesions involved both vertebral body and appendix.Pure osteolysis were found in 26 lesions on CT examinations.Peripheral osteosclerosis rims (41/49) and expansive lesions(32/49) were seen.Residual bone crest(28/49) and ground-glass opacity(23/49)were noted.Different degrees of vertebral compression were found in 19 lesions.Five patients had spinal deformity.On T1WI,14 lesions showed intermediate or low signal,and 10 lesions presented as heterogeneous signal.On T2WI,6 lesions had low signal intensity,4 lesions were noted as hyperintensive,and 14 lesions presented as heterogeneous signal.Multiple fluid-fluid levels were found in 1 lesion.Low signal rims were seen in 14 lesions.Twenty lesions of 11 patients had significant enhancement.Conclusion Expansive pattern,ground-glass opacity,peripheral osteosclerosis rims and significant enhancement were helpful findings for the diagnosis of spinal FD.

Objective To investigate various sex hormones and the their relation in patients with polycystic ovary syndrome (PCOS).Methods 40 patients with PCOS were matched with 40 age matched healthy women,study group was divided into the obese group and non-obese groups based on body mass index,insulin resistance and non-insulin-resistance based on insulin sensitivity index. The level of follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), estradiol (E2), testosterone (T), androstene dione (A4), sulfal-dehydroepiandrosterone (DHEAS), sex hormone-binding globulin (SHBG), fasting glucose(FG), fasting insulin (FIN) was measured in both group. Homeostasis model assessment insulin resistance (HOMA-IR), insulin sensitivity index (ISI) and free androgen index (FAI) were caulculated. Results ①In the PCOS group,LH, LH/FSH, T, A4, FAI, FIN and Homa-IR were significantly higher compared to the control,while FSH, SHBG, ISI were significantly lower (P<0.05),PRL, FG, E2, DHEAS level did not show difference (P>0.05). ②There were significant differences in T, FAI, A4, but not in DHEAS between the PCOS group and the control one. ③There were significant differences between the hirsute group and the non-hirsute group in T, FAI, A4 (P<0.05). ④In the obese PCOS group compared to the non-obese PCOS group, T, A4, DHEAS were not significantly different, but FAI and Homa-IR were significantly higher, SHBG, ISI, LH/FSH were significantly lower. ⑤The quantity of insulin resistance in the increased T group was significantly higher compared to the common T group. ⑥In the insulin resistance group compared to the non insulin resistance group, there were not significant differences of A4、DHEAS,T and FAI was significantly higher.⑦In PCOS group,there were significant positive correlation between FAI useful parameter compared to T, A4, DHEAS for the diagnosis of PCOS. Obese PCOS women have more severe and BMI,FAI and Homa-IR;BMI and LH/FSH were significant inverse correlation. Conclusions FAI is more endocrine secretion and metabolic disturbance than non PCOS women. Hyperandrogenism and insulin resistance are consanguineous correlation.

Objective To isolate and culture meshenchymal stem cells from murine fetal liver.Methods flMSCs from mouse fetuses were isolated by adhering to plastic surface.Growth kinetics was determined by growth curve.Cell cycle and phenotype were analyzed by FACSan flow cytometry.Differentiation of adhering cells was induced and identified.Results Homogenous fibroblast-like cells were predominated in culture.The counting of flMSCs increased 2 fold after 24 hours and 83.76%?2.88% of flMSCs were in G0/G1 phases.flMSCs were CD44,CD29 positive but negative for the markers of hematopoietic cells such as CD45,CD11b.flMSCs were able to differentiate along adipogenic,chondrogenic and osteogenic pathways even after being passaged several times.ConclusionflMSCs can be isolated by their plastic-attachable property and can expand without losing their multiple differentiation potential in vitro.flMSCs may offer an appropriate cell source for stem cell therapy.

ObjectiveIn order to confirm the relationship of pathogenic factors and clinical manifestation of cerebral palsy. Methods185 children were divided into the prematurity group(91 cases) and maturity group(94 cases). The μ-test was applied to analyze the incidence of clinical manifestation of 185 children with cerebral palsy for different pathogenic factors. ResultsThe sever symptoms occurred more frequently in prematurity group than in maturity group. ConclusionThe earlier the careful follow-up for children with prematurity was performed, the earlier the diagnosis and treatment of cerebral palsy were achiened.

BACKGROUND:Studies have found that β-casomorphin 7 can lessen oxidation of renal tubular epithelial cels and lower blood sugar in a rat model of diabetes. There is a few studies concerning the effect of β-casomorphin-7 on diabetic nephropathy. OBJECTIVE:To explore the effects of β-casomorphin-7 on renal injury in a rat model of diabetic nephropathy. METHODS:The 30 rats were equaly and randomly divided into control group, model group andβ-casomorphin-7 group. Twenty rat models of diabetes were established. In the model and β-casomorphin-7 groups, rats were intraperitonealy injected with streptozotocin (60 mg/kg). In the control group, rats were intraperitonealy injectedwith an equal volume of citric acid. Rats in the β-casomorphin-7 group were intragastricaly administeredβ-casomorphin-7 for 30 days. Rats in the model and control groups were given an equal volume of saline by intragastric administration. RESULTS AND CONCLUSION: (1) Compared with the control group, insulin levels decreased, glucagon, blood glucose, urine glucose, urine protein, serum creatinine, serum urea nitrogen levels, mRNA and protein expression levels of type I colagen and type IV colagen in kidney increased in the model group. (2) Compared with the model group, above indexes were apparently recovered in the β-casomorphin-7 group. These findings indicate that β-casomorphin-7 has a protective effect on renal injury in diabetic nephropathy rats.

BACKGROUND: Safflower yel ow is known to treat diabetic nephropathy, can protect kidney function, reduce lesions, delay or prevent the development of diabetic nephropathy.
OBJECTIVE: To further verify the effects of safflower yel ow on the kidney in rats with diabetic nephropathy and the impact of safflower yel ow on the expression of renal cel receptor of angiotensin II1.
METHODS: Thirty rats were equally and randomly divided into three groups: normal control group, model group and experimental group. In the experimental group and model group, rat models of diabetic nephropathy were established. At 1 week after model establishment, rats were daily intraperitoneally given safflower yellow injection 27.8 mg/kg, once a day. In the model and control groups, rats were daily intraperitoneally given an equal volume of saline. All rats were administered for 23 weeks. The kidney-related biochemical indicators and pathological changes in kidney tissue and the expression of angiotensin II1 type receptor on kidney tissue of rats were observed.
RESULTS AND CONCLUSION: (1) Hypertrophy index of experimental group and model group was significantly higher than that of control group (P < 0.05). (2) 24-hour proteinuria, glycosylated hemoglobin, total cholesterol, triglycerides, creatinine and urea nitrogen of model group were higher than that of control group (P < 0.05). 24-hour proteinuria, glycated hemoglobin, total cholesterol and urea nitrogen in experimental group were higher than that of control group (P < 0.05). 24-hour proteinuria, total cholesterol, triglycerides, creatinine and urea nitrogen in the experimental group were lower than that of model group (P < 0.05). (3) Glomerular hypertrophy, thickening of capillary basement membrane, cell proliferation, and mesangial widening were detected in the model group. Abnormal changes in the kidney structure were found in the control group. The extent of damage of kidney of histopathology in the experimental group was between the control group and the model group. (4) The expression level of angiotensin receptor II1 of experimental group and model group was higher than that of control group (P < 0.05). The expression level of angiotensin receptor II1 of experimental group was lower than that of model group (P < 0.05). These results indicated that safflower yellow has a protective effect on diabetic nephropathy; the mechanism of action may be associated with blocking kidney partial renin-angiotensin system.

Objective To explore the MRI diagnostic value for pernicious placental abnormalities.Methods MRI findings of 1 5 patients with pernicious placenta previa were retrospectively analyzed after cesarean section.Results Among all cases,total placenta previa was,1 1 cases and partial placenta previa was 4 cases.The placenta located in the anterior wall in 8 cases,posterior wall in 4 cases,lateral wall in 3 cases.4 cases were diagnosed as normal placenta,6 cases as adhesion,4 cases as implanted,and 1 case as penetrating.These cases had certain special MR features.Conclusion MRI is helpful in diagnosing the location and type of the perni-cious placenta,and showing whether complicated with previa implantation and penetrating.

Objective To investigate the effect of vitamin D on pulmonary function in patients with chronic obstructive pulmona-ry disease,and to explore its possible mechanism.Methods Patients with chronic obstructive pulmonary disease admitted in the hospital were randomly divided into 2 groups,one group of patients were given regular COPD treatment (control group,30 cases), the other group of patients were given vitamin D on the basis of conventional therapy(experimental group,30 cases).Pulmonary function test and C reaction protein (CRP)determination in the 2 groups were performed,the contents of PaO2 ,PaCO2 were also determined.Results Compared with the control group,lung function improvement in experimental group were more obvious,and the the number of acute attack significantly reduced(P <0.05).The concentration of CRP in experimental group was significantly lower after treatment than before treatment(P <0.05),while the concentration of CRP in control group didn′t change significantly (P >0.05).Conclusion Vitamin D contributes to the improvement of pulmonary function in patients with COPD,and its mecha-nism might relate to the reduction of inflammatory reaction.

Objective To explore the methods of inducing human placenta mesenchymal stem cells (MSCs) to differentiate into insulin-producing cells. Methods MSCs were obtained from human placenta and were induced to differentiate into insulin-producing cells by activin A, epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and other cytokines. The cells were identified by immunocytochemistry staining, Western Blot and animal experiment after differentiation. Results After induction, the cells possessed some characteristics of islet βcells, and could express pancreatic duodenal homeobox-1 (PDX-1), insulin and C peptide. The induced cells could also decrease blood glucose levels of diabetic mice. Conclusions These results showed that human placenta MSCs can be induced to differentiate into insulin-producing cells by cytokines.

AIM: To explore the possibility of differentiating functional insulin-producing cells from human BM-derived stem cells. METHODS: Mesenchymal stem cells were isolated from human bone marrow. Then these cells were induced with epidermal growth factor, β-mercaptoethanol and high concentration of glucose. The gene expression related to islet β cells was detected by RT-PCR. Insulin in the treated cells was examined by immunocytochemistry. In addition, the levels of insulin secretion and glucose-stimulated insulin release were examined by microparticle enzyme immunoassay. Finally, the induced cells were implanted into the right renal subcapsular space of diabetic mice. Blood glucose levels were monitored 16 d after implantation. The right kidneys of the treated mice were harvested for immunohistochemistry. RESULTS: The key genes related to pancreatic β cells had been confirmed to express by PCR and insulin was detected by immunocytochemistry in differentiated human BM-derived stem cells induced by high glucose, which responded to glucose challenge. Furthermore, implantation of the cells in renal subcapsular space was able to lower the glucose levels in hyperglycemic mice. After 16 days, the implanted cells were determined still to be insulin positive cells by immunohistochemistry. CONCLUSION: These results indicate human BM-derived stem cells are capable of differentiating into functional insulin-producing cells and may represent a pool of cells for the treatment of diabetes.