1.Establishment of a rat model of gut hypersensitivity and for evaluation of visceral sensitivity
Yanbing LIU ; Yaozong YUAN ; Ranjun TAO
Chinese Journal of Digestion 2001;0(01):-
Objective Visceral hypersensitivity is an important feature of irritable bowel syndrome (IBS). This investigation was to establish an animal model of visceral hypersensitivity associated with IBS, and to test its effectiveness by two different ways. Methods The model was established by intrarectal administration of acetic acid daily in the neonatal rats between postnatal 8 and 21 days. The threshold of abdominal withdrawal reflex(AWR) was evaluated during rectal distension at postnatal 6,8 and 10 weeks respectively, and the changes of rectal sensitivity were identified by the abdominal electrical activity measured at postnatal 12 weeks. Results In contrast to neonatal rats subjected to saline intrarectally (NS group) and adult rats subjected to acetic acid intrarectally (AA group), neonatal rats subjected to acetic acid intrarectally(NA group) showed a significant decrease(P
2.Research progress in diagnosis and treatment of pancreatic encephalopathy
Yuan LIU ; Tao LI ; Shengchun DANG
Journal of Clinical Surgery 2015;(11):867-869
Pancreatic encephalopathy(PE)is one of the severe complications in acute pancreati-tis,which is characterized by a group of neurological signs and symptoms. Itˊs very difficult to have an early diagnosis,while the morality of PE is very high and the prognosis is very poor. Eliminating causes of pan-creatic encephalopathy,early diagnosis and combined therapy are the keys to achieve good curative effects. This paper reviewed the recent progress in the main mechanisms,clinical manifestations,diagnosis and therapy of PE.
3.Antitumor activity of different organic solvents extracts and plumbagin from plumbago zeylanica L. on EMT-6 breast cancer and transplanting S180 in vivo
Jizhong ZHANG ; Yuan LIU ; Tao JIAO
Chinese Pharmacological Bulletin 2003;0(08):-
Aim To find out the effect of the different organic solvents extracts and plumbagin from Plumbago zeylanica L.on EMT-6 breast cancer of BALB/C mouse and transplanted S180 of KM mouse primarily.Method Experiments of animal transplanted tumor in vivo were adopted.Results ① The high dose of chloroform group and plumbagin group could inhibit the growth of EMT-6 breast cancer in BALB/C mice in vivo.Compared with that of physiological saline group,tumor weight has obviously lightened(P
4.Evaluation of growth and reproduction of CL Long Evans rats
Zhonglu LIU ; Jun YUAN ; Cuilan TAO
Journal of Third Military Medical University 2003;0(17):-
Objective To study the growth and development as well as reproductive capacity of CL Long Evans rats. Methods Eighty CL Long Evans rats(80 days old)were bred in a barrier system. A monogamous mating system is used to reproduce 5 litters. Their main indexes of growth and reproduction were determined. The body weight gain of 60 second litter rats were analyzed from day 20 to 100. Results The number and weight of the fetus, the weaning time weight per cote and per litter in the second litter were the highest, and the fifth litter was the lowest. The growth and development curve was the same as that announced by Taconic. Conclusion Long Evans rats have been confirmed suitable for continual reproduction for 4 litters and the second litter is the best one for mating.
5.Expressions and significance of Vimentin and E-cadherin during epithelial-mesenchymal transition of hepatocellular carcinoma tissue
Lei CHANG ; Yufeng YUAN ; Tao GUO ; Quanyan LIU ; Zhisu LIU
Chinese Journal of Hepatobiliary Surgery 2015;21(1):9-13
Objective To investigate the molecular expression and clinical significance of Vimentin and E-cadherin during epithelial-mesenchymal transformation of hepatocellular carcinoma (HCC) tissues.Methods Hepatocellular carcinoma and paracancerous tissue were collected from 55 patients,while nonneoplastic nonnal liver tissue were collected from 20 patients without cancerous diseases as control group.Expressions of Vimentin and E-cadherin were examined by immunohistochemistry staining.Clinicopathological features of the relative expression of Vimentin and E-cadherin,and its influence on prognosis were discussed as well.Results Vimentin was positively expressed in 69.1% cancer tissue,and was significantly higher than paracancerous tissues and normal liver tissues(P < 0.05).E-cadherin was positively expressed in 27.3% cancer tissue and was significantly lower compared with paracancerous tissues and normal liver tissues(P <0.05).The expression of Vimentin and E-cadherin was negatively correlated(r =-0.653,P < 0.05).Vimentin expressions in subgroups of TNM stage Ⅲ-Ⅳ,poor differentiation,multiple tumor nodules,metastasis and recurrence were all significantly higher than the respective subgroups as TNM stage Ⅰ-Ⅱ] (x2 =7.267,P < 0.05),moderate to well differentiation (x2 =4.045,P < 0.05),single tumor node (x2=12.143,P <0.05),without metastasis or recurrence (x2 =7.267,P < 0.05).On the contrary,Ecadherin showed higher expression in the subgroup of single tumor node compared with multiple tumor nodules(x2 =6.878,P < 0.05).The follow-up of patients further demonstrated that patients with positive Vimentin expression had a significantly lower disease-free survival compared with the Vimentin negative group (P <0.05).Conclusions Vimentin and E-cadherin both participated in epithelial-mesenchymal transition process of hepatocellular carcinoma.High expression of Vimentin and low expression of E-cadherin were closely correlated with occurrence and development of HCC.Vimentin and E-cadherin may serve as impottant biomarkers in clinical diagnosis and prognosis prediction of HCC.
6.Cerebral protection effect and mechanism of propofol on global cerebral ischemia and reperfusion damage in rats
Tao WANG ; Xiao-yuan LIU ; Ji-zong ZHAO ; Shuzhen ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2004;10(8):464-465
ObjectiveTo determine the cerebral protection effect and mechanism of propofol on global cerebral ischemia and reperfusion damage in rats.Methods19 adult male Wistar rats were randomly divided into 3 groups, ischemia group (n=7), propofol group (n=7), and sham injury group (n=5). Global cerebral ischemia and reperfusion model were made by means of Pulsinelli's method. Rats in propofol group were anesthesia with propofol at the dosage of 1.5 ml/h for 30 min at the beginning of reperfusion. Apoptosis and necrosis rate were detected by cytometry. In the same time, bcl-2, Bax and p53 protein expression in hippocampus neurons were detected. ResultsThe apoptosis and necrosis rate in propofol group were significantly decreased as compared with ischemia group ( P<0.05). Bax and p53 protein expression in hippocampus neurons were also significantly decreased as compared with ischemia group (P<0.05), however, no significant findings in bcl-2 protein expression (P>0.05).ConclusionPropofol can decrease apoptosis and necrosis rate in cerebral ischemia reperfusion injured neuron, and the mechanism maybe related to decreasing the expression of Bax, p53 protein.
7.Segmental anterior cervical decompression with fusion for treating multilevel cervical myelopathy: Comparison of fusion rates among three methods
Wen YUAN ; Shengming XU ; Xinwei WANG ; Tao ZHANG ; Baifeng LIU
Chinese Journal of Tissue Engineering Research 2007;11(47):9595-9598
BACKGROUND:As a traditional treatment for multilevel cervical myelopathy,nterior long-segmental decompression has the shortcomings of great operative trauma,high difficulty,low fusion rate,etc.,which can affect the postoperative efficacy.OBJ ECTIVE:To evaluate the clinical effects of three different anterior surgeries on multilevel cervical myelopathy.DESIGN:A comparative observation.SETTING:Department of Orthopaedics,Changzheog Hospital,the Second Military Medical University of Chinese PLA.PARTICIPANTS:Thirty-six patients with multilevel cervical myelopathy of 3 consecutive segments,who were surgically treated,were selected from the Department of Orthopaedics,Changzheng Hospital,the Second Military Medical University of Chinese PLA from June 1999 to June 2003,including 25 males and 11 females,35-62 years of age,the disease course ranged from 3 to 26 months. According to the clinical manifestations and imaging esults,they were diagnosed as multilevel cervical myelopathy,and they were not suffering from consecutive ossification of posterior longitudinal ligament and ossification of ligamenta flava. Informed contents were obtained from all the patients and their relatives.METHODS:All the patients were grafted with utologous bone. Autologous ilium or cancellous bone excluding vertebral body was filled into titan net or Cage,which were made of titan and characterized by high intensity,tolerance to decay,good biocompatibility,etc. According to the operative manner,the patients were divided into 3 groups:① two-level corpectomy with fusion group(long-segmental decompression group,n =11):There were 4 cases grafted with long-titan net,and 7 cases grafted with autologous iliac bone. Sub-total two-level corpectomy with fusion was performed. ②segmental decompression group(n =16):including 12 cases of titan net+cage graft,4 cases of autologous bone+cage graft. One-level decompression and sub-total single corpectomy with fusion were performed. ③three-level decompression group(n =9):Only discectomy without corpectomy was performed. After complete decompression,3cages were used to fill artificial bone or grafted with autologous bone.MAIN OUTCOME MEASURES:Cervical anteroposterior and lateral radiographies,flexion and extension radiograph were reexamined within 1 week and at 3,6 and 12 months postoperatively. The neurological function was assessed using the Japanese Orthopaedic Association(JOA) scoring method preoperatively and 3 months postoperatively. The total score was 17 points,the higher the score,the better the neurological function. The duration of operation,perioperative bleeding amount,length of stay,cost of hospitalization,graft fusion at 3 months postoperatively,improved JOA score at 3 months postoperatively were recorded in the three groups. The occurrence of postoperative complications was observed by means of return visit.RESULTS:All the 36 patients with multilevel cervical myelopathy were involved in the analysis of results. The mean duration of operation,mean perioperative bleeding amount and mean length of stay in the segmental-decompression group and three-level decompression group were obviously fewer or shorter than those in the long-segmental decompression group(P < 0.05),and the average cost of hospitalization was obviously higher than that in the long-segmental decompression group(P < 0.05). The postoperative improved JOA score and graft fusion rate were close among the groups(P > 0.05).CONCLUSION:Segmental anterior cervical decompression is a recommendable technique for multilevel cervical myelopathy by comprehensively considering the fusion rate,recovery of neurological function,duration of operation,perioperative bleeding and length of stay.
8.Effect of propofol on endothelial nitric oxide synthase and inducible nitric oxide synthase expression in thoracic aorta of hypertensive rats
Yaling LIU ; Yuan MA ; Yisa SHI ; Tao LI ; Yibo GAO
Chinese Journal of Anesthesiology 2011;31(8):922-925
ObjectiveTo investigate the effect of propofol on endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) expression in thoracic aorta of hypertensive rats.MethodsHealthy SD rats of both sexes weighing 240-280 g were used in this study.Hypertension was induced by subcutaneous deoxycorticosterone 25 mg/kg twice a week for consecutive 7 weeks.Sixty-four hypertensive rats were randomly divided into four groups (n = 16 each):hypertension group (group H),low,medium and high dose propofol group ( groups P1,P2,P3 ).Groups P1,P2 and P3 received infusion of propofol at a rate of 20,30 and 40 mg* kg- 1 · h- 1 for 3 h respectively,while group H received equal volume normal saline instead of propofol.Mean arterial pressure (MAP) was monitored and recorded before,1 h and 3 h after the start of propofol or normal saline infusion.All animals were sacrificed at 3 h of intravenous administration.Blood samples were collected by taking out the eyeballs for determination of serum NO concentrations by nitrate reductase method.The expression of eNOS mRNA,iNOS mBNA was determined by reverse transcription-polymerase chain reaction.The expression of eNOS and iNOS protein was determined by Western blot.ResultsCompared to group H,MAP was decreased significantly,the serum NO concentrations were increased significantly,the expression of eNOS mRNA and protein in thoracic aorta was up-regulated,and the expression of iNOS mRNA and protein in thoracic aorta was down-regulated in a dose-dependent manner in groups P1,P2 and P3 ( P < 0.05 or 0.01 ).ConclusionPropofol can down-regulate iNOS expression and up-regudate eNOS expression in endothelial cells of thoracic aorta and promote NO release in hypertensive rats,Which is the mechanism of propofol decreasing pressure.
9.Effects of vitamin E on mercuric chloride-induced renal interstitial fibrosis in rats and the antioxidative mechanism.
Yanyan TAO ; Qinglan WANG ; Jili YUAN ; Li SHEN ; Chenghai LIU
Journal of Integrative Medicine 2011;9(2):201-8
To observe the effects of vitamin E (Vit E) on mercuric chloride (HgCl2)-induced renal interstitial fibrosis (RIF) in rats and discuss its antioxidative mechanism.
10.Role of autophagy in the motivation of rat myocardial hypertrophy in H9c2 cells induced by bone morpho-genetic protein 4 and its mechanism
Yezhen TAO ; Jianhua LI ; Yakun LIU ; Peipei WANG ; Yu YUAN
Journal of Medical Postgraduates 2016;29(7):718-722
Objective Bone morphogenetic protein 4 ( BMP4) induces rat myocardial hypertrophy in H9c2 cells, but the spe-cific mechanism remains unclear .The study aimed to elucidate the role of autophagy in myocardial hypertrophy and its relationship with extracellular signal regulating kinase ( ERK1/2) signal transduction pathway . Methods H9c2 cardiomyocytes were randomly divided into 4 groups:control group, BMP4 group, BMP4+PD98059 (ERK1/2 signal pathway inhibitor) group and BMP4+3MA (autophagy inhibitor) group.With PD98059(50μmol/L) and 3MA(5mmol/L) blocking for 30min, BMP4 (50μg/L) were added.Expressions of tiny tube related proteins 1 light chain 3 (LC3) and p-ERK1/2 were detec-ted after culturing for 30min.48h later, measurements were made on cell surface area , average protein content and α-smooth muscle actin (α-SMA ) protein expression level .Cell morphology and size were measured respectively by inverted microscope and Image J software .Total protein content was detected by BCA , and western blot was used to measure the expressions of LC3, ERK1/2, p-ERK1/2 and α-SMA. Resul ts 30min later, the expressions of LC3 and p-ERK1/2 were significantly higher in BMP4 group than in control group [(1.54 ±0.05) vs (1.95 ±0.11),(0.94 ±0.04) vs (1.33 ± 0.06),P<0.01] and no significant difference was found in other two groups .Compared with BMP4 group, significant decrease was found in BMP4+PD98059 and BMP4+3MA [(1.95 ±0.11) vs (1.59 ±0.08), (1.36 ±0.02);(1.33 ±0.06) vs (0.87 ±0.05), (0.95 ±0.15), P<0.01], while no significant difference was found between BMP 4+PD98059 group and BMP4+3MA group.48h lat-er, the cell area, protein content and α-SMA protein expression level are significantly lower in control group than in BMP 4 group [(644.1 ±15.01)μm2 vs (745.6 ±14.43)μm2,(240.0 ±7.26)pg/cell vs (347.1 ±5.4)pg/cell,(1.22 ±0.06 vs 1.99 ±0.03),P<0.01], while the corresponding indexes were significantly higher in BMP 4+PD98059 and BMP4+3MA compared with BMP4 group [(745.6 ±14.43)μm2 vs (645.0 ±12.63)μm2, (647.7 ±13.89)μm2;(347.1 ±5.4)pg/cell vs (239.7 ±3.39)pg/cell , (241.1 ± 8.56)pg/cell;(1.99 ±0.03) vs (1.13 ±0.05);(1.12 ±0.02), P<0.01].No significant difference was found as to the indexes be-tween BMP4+PD98059 group and BMP4+3MA group(P>0.05). Conclusion Autophagy may participate in BMP4-induced rat myo-cardial hypertrophy in H9c2 cells through ERK1/2 signal transduction pathway .The clinical treatment of myocardial hypertrophy may benefit from the blocking of autophagy or ERK 1/2 signal transduction pathway .