1.Endoscopic treatment of benign ureterointestinal anastomotic strictures in patients with urinary diversion
Guohua ZENG ; Wen ZHONG ; Houmeng YANG ; Zhiming GUI ; Wenzhong CHEN ; Jian YUAN
Chinese Journal of Urology 2009;30(6):408-410
Objective To evaluate the clinical efficacy of endourologic treatment of benign uret-erointestinal anastomotic strictures in patients with urinary diversion. Methods Nine cases of benign ureterointestinal anastomotic strictures with a length of 1-3 cm following radical cystectomy and uri-nary diversion accepted endourologic treatment. 8 cases were treated by antegrade percutaneous ap-proach, 1 case by retrograde ureteroscopic approach. The strictures received balloon dilation, and ure-teral stents indewelled. Results In a follow up of 0.5-5.0 years, 1 case received percutaneous ne-phrostomy for complete ureterointestinal anastomotic atresia and refused to open operation reconstruc-tion. 5 cases had no recurrence after 2-3 endoscopic sessions. 3 cases needed long time ureteral stents indwelled. Conclusion Endourological technique for ureterointestinal strictures following urinary di-version avoided the disadvantages of open operation.
2.Effect of balloon angioplasty on plasma ET and TNF-α levels and tissue endothe lin immunoreactivity in experimental atherosclerotic rabbits
Rong-Zeng DU ; Zong-Gui WU ; Zuo HUANG ; Gao-Zhong HUANG ; Guo-Yuan ZHANG
Academic Journal of Second Military Medical University 2001;22(2):130-132
Objective: To assess the effect of balloon angiopl asty on circulating endothelin (ET) and TNF-α levels and tissue endothelin in experimental atherosclerosis in rabbits. Methods: After 20 New Z ealand rabbits had a high cholesterol diet for at least 8 weeks, successful ball oon angioplasty was performed in rihgt iliac arteries in 18 rabbits. Circulatin g levels of ET and TNF-α were measured before as well as immediately and 24 h after balloon angioplasty. Tissue endothelin immunoreactivity in atherosclerotic iliac artery wall after balloon angioplasty was assessed by immunohistochemica l technique. Results: Plasma levels of ET and TNF-α were signi ficantly increased immediately after ballon angioplasty (76.40±13.58)pg/ml vs (92.67±11.38) pg/ml and (31.35±6.23) U/ml vs (56.26±7.37) U/ml, resp ectively (P<0.05) .There was no change in plasma ET and TNF-α levels 24 h after balloon angioplasty (77.13±12.87) pg/ml vs (76.40±13.58) pg/ml and (33.41±6.79) U/ml vs (31.35±6.23) U/ml, respectively (P>0.05). T issue endothelin immunoreactiuvity was markedly increased in right iliac artery wall after balloon angioplasty than that in opposite iliac artery wall. Conclusion: The increase of plasma ET, TNF-α levels and tissue ET-IR in iliac artery wall after balloon angioplasty may be associated with the injury of l ocal vascular intima, suggesting that ET and TNF-α may take part in the corona ry constriction and the development of coronary restenosis after percutaneous tr ansluminal coronary angioplasty.
3.Metabonomics Study on Urine 1H-NMR in Chronic Superficial Gastritis Patients with Pi-qi Deficiency Syndrome/Pi-Wei Dampness-heat Syndrome.
Xu-guang SHI ; Zhong-jie ZOU ; Mei-yin WU ; Yuan-gui ZENG ; Zhi-cheng LIAN ; Man-ting HUANG ; Meng-juan GONG
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(12):1427-1432
OBJECTIVETo observe metabolomic changes in urine of chronic superficial gastritis (CSG) patients with Pi-qi deficiency syndrome (PQDS) or Pi-Wei dampness-heat syndrome (PWDHS), thereby providing scientific evidence for syndrome typing of them.
METHODSUrine samples were collected from CSG patients with PQDS/PWDHS and healthy volunteers, 10 in each group. Proton nuclear magnetic resonance spectroscopy (1H-NMR) based metabonomic analysis was performed on urine samples. Contents of related biomarkers were analyzed by principal component analysis (PCA), partial least square discriminant analysis (PLS-DA), and urivariate statistical analysis.
RESULTSPLS-DA analysis showed that metabolites among CSG patients with PQDS/PWDHS and healthy volunteers could be mutually distinguished. Seven differentially identified metabolites were screened from urines of CSG patients with PQDS and healthy volunteers included glutamate, methionine, α-oxoglutarate, dimethylglycine, creatinine, taurine, and glucose. Four differentially identified metabolites were screened from urines of CSG patients with PWDHS and healthy volunteers included 2-hydroxybutyric acid, trimethylamine oxide, taurine, and hippuric acid. Eleven differentially identified metabolites were screened from urines of CSG patients with PQDS and PWDHS included fucose, β-hydroxybutyric acid, alanine, glutamate, methionine, succinic acid, citric acid, creatinine, glucose, hippuric acid, and lactic acid.
CONCLUSIONThe metabolic differences of CSG patients PQDS and PWDHS mainly manifested in glycometabolism, lipid metabolism, and amino acids catabolism, and 1H-NMR based metabonomics may be used in classified study of Chinese medical syndrome typing.
Biomarkers ; urine ; Discriminant Analysis ; Gastritis ; urine ; Hot Temperature ; Humans ; Hydroxybutyrates ; Ketoglutaric Acids ; Least-Squares Analysis ; Medicine, Chinese Traditional ; Metabolome ; physiology ; Metabolomics ; Principal Component Analysis ; Proton Magnetic Resonance Spectroscopy ; Qi ; Syndrome
4.The change of coronary circulating TNF-α level in patients with coronary heart disease after PTCA and its significance
Rong-Zeng DU ; Zong-Gui WU ; Zuo HUANG ; Gao-Zhong HUANG ; Guo-Yuan ZHANG
Academic Journal of Second Military Medical University 2001;22(2):185-186
Objective:To observe the effect of percutaneous tr ansluminal coronary angioplasty (PTCA) on coronary circulating tumor necrosis fa ctor-α (TNF-α) activity. Methods: Plasma TNF-α levls were measured with radioimmunoassay and bioactive assay respectively. Result s: Plasma TNF-α activity in femoral artery (AO) was significantly incr eased immediately after PTCA [(15.86±3.75) U/ml vs (41.32±4.36) U/ml, P<0.01], and plasma TNF-α activity in coronary sinus was remarkably incre ased immediately after PTCA [(16.72±4.14) U/ml vs (65.61±6.25) U/ml, P<0.01]. There was no change in plasma TNF-α activity in AO 24 h after PT CA [(18.32±5.12) U/ml vs (15.86±3.75) U/ml, P>0.05]. Conclu sion: The increase in plasma TNF-α activity after PTCA may be associat ed with the injury of coronary artery caused by PTCA, suggesting that TNF-α ma y be involved in the coronary occlusion and the development of coronary restenos is after PTCA.
5.Tranilast inhibits myocardial fibrosis in mice with viral myocarditis.
Chun WEN ; Gui XIE ; Ping ZENG ; Lin-Feng HUANG ; Chun-Yuan CHEN
Chinese Journal of Contemporary Pediatrics 2016;18(5):446-454
OBJECTIVETo investigate the effect of tranilast on myocardial fibrosis in mice with viral myocarditis (VMC).
METHODSMale balb/c mice (n=72) were randomly divided into control, VMC and tranilast groups (n=24 each). In the VMC and tranilast groups, the mice were infected with Coxsackie virus B3 (CVB3) to prepare VMC model, while the control group was treated with Eagle's medium. After modeling, the tranilast group was administrated with tranilast [200 mg/(kg.d)] until the day before sampling. On days 7, 14 and 28 after CVB3 or Eagle's medium infection, heart specimens (n=8) were taken and examined after Toluidine blue staining and Nissl staining for counts of mast cells (MC), hematoxylin-eosin staining for myocardial pathological changes, and Masson staining for myocardial fibrosis. The expression of CTGF and type I collagen (Col I) in the myocardial tissue was measured by RT-PCR and Western blot. The correlations of CTGF mRNA expression with MC counts and Col I expression were analyzed.
RESULTSThe myocardial pathological changes and collagen volume fraction in the VMC group were significantly higher than in the control group at all three time points (P<0.05). Tranilast treatment significantly decreased the myocardial pathological changes and collagen volume fraction compared with the VMC group (P<0.05). The mRNA and protein expression of CTGF and Col I increased in the VMC group compared with the control group, and the increases were reduced with tranilast treatment (P<0.05). The number of MC was positively correlated to CTGF mRNA expression on the 7th day and 14th day (r=0.439, P=0.049) in the VMC group. There were positive correlations between the mRNA expression of Col I and CTGF on the 7th day and 14th day (r=0.646, P=0.007) and the 28th day (r=0.326, P=0.031).
CONCLUSIONSTranilast may inhibit the aggregation of MC and down-regulate the expression of CTGF, relieving myocardial fibrosis of mice with VMC.
Animals ; Collagen Type I ; genetics ; Connective Tissue Growth Factor ; genetics ; Coxsackievirus Infections ; drug therapy ; Enterovirus B, Human ; Fibrosis ; Male ; Mice ; Mice, Inbred BALB C ; Myocarditis ; drug therapy ; Myocardium ; pathology ; RNA, Messenger ; analysis ; ortho-Aminobenzoates ; pharmacology
6.Comparative effects of valsartan and extended realse nifedipine tablets on lowering blood pressure and reversing left ventricular hypertrophy
Yuan-Zhou LI ; Li-Xian YANG ; Ze-Feng LV ; Si-Gan ZHONG ; Rong-Hui ZENG ; Gui-Zhong LIU ; Ai-Wen CHEN ; Chun-Yun ZHANG ;
Chinese Journal of Primary Medicine and Pharmacy 2005;0(11):-
Objective To study the different effects of valsartan and extended realse nifedipine tablets on lowering blood pressure of essential hypertension patients and their reversal effects on left ventricular hypertrophy. Methods 100 cases of essential hypertension patients with left ventricular hypertrophy were randomly divided into valsartan group(group A) and adalt group(group B).Other antihypertensive drugs except diuretic were removed for 3 weeks.There were 50 cases in group A using valsartan 4~8mg qd,and 50 cases in group B using adalt 30~60 mg qd,the stud),lasted for 24 weeks.The blood pressure was measured and the altrasowic cardiogram examed in baseline and 24 weeks later.Results BP could be significantly reduced after treatment(P
7.Tolerance and pharmacodynamics phase Ⅰ clinical trial study of chimeric anti-CD20 monoclonal antibody IBI301 in Chinese patients with CD20-positive non-Hodgkin's lymphoma.
Bo JIANG ; Jun Yuan QI ; Ming Yuan SUN ; Zeng Jun LI ; Wei LIU ; Li Jun LIU ; Feng Kui ZHANG ; Lu Gui QIU
Chinese Journal of Hematology 2018;39(4):320-324
Objective: To evaluate the tolerance and safety of a human-mouse chimeric anti-CD20 monoclonal antibody IBI301 in Chinese patients achieved objective response with CD20(+) B-cell non-Hodgkin's lymphoma (NHL). Methods: Nine patients with CD20(+) B-cell NHL received dose-escalating IBI301 infusions (250 mg/m(2), n=3; 375 mg/m(2), n=3; 500 mg/m(2), n=3, respectively). The data of all patients were collected for safety analyses. The median exposures of 125 mg/m(2), 375 mg/m(2), 500 mg/m(2) dose groups were 243, 690 and 980 mg, respectively. Safety and tolerability were evaluated by monitoring adverse events (AE). The ratios of CD19(+), CD20(+) B cells and the levels IgG and IgM were detected to evaluate the pharmacodynamics. Results: Totally 52 events of AE were observed, including 18 events of AE in 125 mg/m(2) group, 14 events of AE in 375 mg/m(2) group and 20 events of AE in 500 mg/m(2) group, respectively. There were 26 adverse reactions of 52 cases of AE, 22 reactions were judged to be probably related to IBI301, and 4 reactions were not probably related to IBI301, all disappeared or returned to baseline levels. Common AE in this study included decreased WBC, upper respiratory infection, decreased neutrophil count, dyspepsia, hyperuricemia, paresthesia, oral mucositis and dizziness. No patients quitted or trial discontinued. No severe AE (SAE) were reported. No dose-limiting toxicity (DLT) events were observed in the study. The ratio of CD20(+) and CD19(+) B cells decreased in all subjects. There was no significant changes of the levels of IgG and IgM. Conclusions: The single dose of IBI301 injection was well tolerated, and the AE occurred in the patients recovered. No SAE were reported, No DLT events were observed in the study. The IBI301 caused an elimination of the peripheral CD20-expressing B cells in all patients. Clinical trial registration: Chinadrugtrials, CTR20140762.
Adult
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Animals
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Antibodies, Monoclonal
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Antigens, CD20
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Antineoplastic Agents
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Child
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Humans
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Lymphoma, B-Cell
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Lymphoma, Non-Hodgkin/drug therapy*
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Mice
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Rituximab
8.Investigation of current recognition of and demand for health knowledge by patients with chronic gastritis receiving treatment in clinics
Zhen ZHENG ; Hui-Kun WU ; Mei-Yuan YAN ; Gui-Zhen ZENG ; Yuan-Xi HUANG ; Gang LIANG ; Ping-Hu CHEN ; Xiao-Hong LU ; Xia-Hua CHEN
Chinese Journal of Modern Nursing 2009;15(22):2146-2148
cation according to different requirement of patients so as to help the patients to change their had behavior for the prevention and the reduction of the recurrence and the complication of the disease.
9.Treatment of urinary tract calculi in preschool children with endoscopy.
Guo-Hua ZENG ; Wen ZHONG ; Hou-Meng YANG ; Zhi-Ming GUI ; Wen-Zhong CHEN ; Jian YUAN
Chinese Journal of Surgery 2009;47(4):252-254
OBJECTIVESTo investigate the safety and feasibility of endoscopy in treating urinary tract calculi in preschool children.
METHODSFrom August 2004 to August 2008, 28 preschool children with urinary tract calculi were treated by endoscopy, 11 cases received ureterolithotripsy (URL) and 17 cases received minimally invasive percutaneous nephrolithotomy (MPCNL).
RESULTSOf 11 cases with ureteric calculi, 5 cases were rendered stone free in the first session, the other 6 cases received passive dilation by indwelling of ureteric stents for 1 to 3 weeks and underwent successful ureteroscopy with a 8/9.8 Fr rigid ureteroscope. Seventeen cases with renal calculi received MPCNL and were rendered stone free.
CONCLUSIONOur study shows that endoscopy in treating urinary tract calculi is safe and feasible in preschool children.
Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Nephrostomy, Percutaneous ; Treatment Outcome ; Ureteroscopy ; Urinary Calculi ; surgery
10.Effect of ginsenoside Rg1 on expression of p21, cyclin E and CDK2 in the process of cell senescence.
Chao-hui ZHAO ; Xiao-chun CHEN ; Jian-sheng JIN ; Yuan-gui ZHU ; Guang-bin SHI ; Yu-qi ZENG ; Yong-kun LI ; Xu PENG
Acta Pharmaceutica Sinica 2004;39(9):673-676
AIMTo explore the possible role of p21, cyclin E and cyclin-dependent kinase 2 (CDK2) in the protection of ginsenoside Rg1 against tert-butylhydroperoxide (t-BHP)-induced senescence in WI-38 cells.
METHODSThe cellular ultrastructure, cytometric assay and beta-galactosidase (beta-gal) cytochemistry staining were used to evaluate cell senescence. The levels of of p21, cyclin E and CDK2 protein were detected by Western blot.
RESULTSPretreatment with Rg1 significantly attenuated t-BHP-induced senescence in WI-38 cells. Simultaneously, compared with cells treated with t-BHP alone, Rg1 pretreatment markedly decreased the level of p21 protein and increased the levels of CDK2 and cyclin E.
CONCLUSIONp21, cyclin E and CDK2 may be involved in the process of ginsenoside Rg1 protection against t-BHP-induced senescence in WI-38 cells.
CDC2-CDC28 Kinases ; metabolism ; Cell Line ; Cellular Senescence ; drug effects ; Cyclin E ; metabolism ; Cyclin-Dependent Kinase 2 ; Fibroblasts ; cytology ; metabolism ; Ginsenosides ; isolation & purification ; pharmacology ; Humans ; Panax ; chemistry ; Plants, Medicinal ; chemistry ; Proto-Oncogene Proteins p21(ras) ; metabolism ; tert-Butylhydroperoxide ; antagonists & inhibitors