Objective To study the stress resistance of Rhodiola under freezing injury stress. Methods With R.kirilowii, R.crenulata, R.Algida and R.fastigiata as the experimental materials, the effects of freezing injury stress on emerging time, duration of seedling emergence, emergence rate, surviving rate, the activity of SOD and POD were observed by field test and experimental detection. Results Before seeding, the effect of freezing injury stress on R.kirilowii was the largest, and that on R.fastigiata was the least. When injury stress happened during seedling stage, surviving rate, increase of SOD and POD activities of R.kirilowii was the least, indicating the freezing injury was severe. R.fastigiata was the best in those indexes. The stress resistance of Rhodiola was as follows:R.fastigiata > R.crenulata > R.Algida > R.kirilowii. Conclusion R. fastigiata and R.crenulata are good choice in introduction and cultivation of Rhodiola in Gannan area of Gansu province, and R.fastigiata is the best.

Encephalocele ; Humans ; Infant, Newborn ; Meningocele ; Nasopharynx

Bartter Syndrome ; complications ; physiopathology ; Hoarseness ; etiology ; Humans ; Infant ; Male

Objective To investigate the feasibility of using ultrasound‐mediated destruction of microbubbles ( US+ MB) to enhance the transplantation of endothelial progenitor cells ( EPCs) to confer chronic allograft vasculopathy (CAV) .Methods Bone marrow derived mononuclear cells were isolated and induced in vitro . The abdominal aorta transplantation was performed . Four groups were divided:control group without treatment (group A) ,injection with saline (group B) ,injection with EPCs (group C) ,group D ( US+MB+EPCs) was injected with EPCs and US was applied to MB prior to the infusion . All rats were killed during 8 weeks after transplantation to enable histological examination;SDF‐1α expression was detected by immunohistochemistry ,the expression of SDF‐1αand TNF‐αin the grafted aortas were detected with RT‐PCR . Results When 8 weeks after EPCs transplantation ,there was a significant improvement in aortic intima of Group D compared with Group B and C ,respectively ( P <0 .05) . In addition ,treatment of Group D significantly increased the expression of SDF‐1αand reduced the expression of TNF‐αin the grafted aortas . Conclusions US‐mediated MB destruction prior to EPCs transplantation into the grafted aortas can improves the effectiveness of endothelial repair and delay the progress of CAV .

Female ; Humans ; Infant, Newborn ; Kidney Neoplasms ; Twins, Monozygotic ; Wilms Tumor

Background and purpose: Urokinase-type plasminogen activator receptor is related to invasion and metastasis of tumor. Inhibition of uPAR expression in tumor cells results in reducing its metastasis. This study was aimed to construct an expression vector with short hairpin RNA (shRNA) of uPAR, which could pave the way for RNAi-mediated tongue squamous cell carcinoma therapy. Methods: Genome sequences of uPAR gene were retrieved from Genhank and cDNA was designed to code expression of shRNA for uPAR gene. The cDNA was synthesized and inserted into the eukaryotic expression vector pWH1, and the recombinant pWH1-uPAR expression vector was identified by enzyme cutting method. Then, pWH1-uPAR expression vector was transfected into tongue squamous cell carcinoma Ts cells by Lipofectomine 2000. At last, the expression of uPAR in Ts cells transfected with pWH1-uPAR expression vector was observed by RT-PCR, immunocytochemistry staining and Western blot. MTT assay was performed to measure the proliferation of Ts cell. Results: The uPAR shRNA eukaryotic expression vector was successfully constructed. Compared with Ts cells and Ts cells transfected with plasmid pWH1, the Ts cells transfected with pWHI-uPAR expression vector showed a lower mRNA and protein expression of uPAR. The inhibition rate of proliferation was 32.9% of Ts cells by transfected with pWHl- uPAR. Conclusion: The constructed uPAR shR.NA expression vector could inhibit the expression of uPAR in tongue squamous cell carcinoma, which may be helpful for further research on the function of uPAR and provide effective methods for therapy of tongue squamous cell carcinoma.

Objective To estimate the quality of HIV 1/2 antibody labeled ELISA Kit made in China by indirect method and dual antigen sandwich method. Methods Eight kits were divided into 4 groups according to the manufacturers (indirect method and dual antigen sandwich method for the same manufacturer in one group) and were used for the determination of 100 serum samples. The positive and suspected positive results were affirmed by Western blot analysis. Results The sensibility, efficacy ratio and NPV range of indirect method were 97.97%～100%, 93.49%～98.04% and 97.05%～100%, respectively, but 2 kits failed to detect the HIV positive sample. The sensibility, efficacy ratio and NPV range of dual antigen sandwich method were 100%, 96.15%～100% and 100% without mis diagnosis. Conclusion Dual antigen sandwich method is better than indirect method in some indexes of quality.

Objective To screen for serum protein differentially expressed between women whose fetuses had congenital heart defects(CHD) and women who had normal fetuses. Methods Serum samples were collected from pregnant women whose fetuses had CHD and those whose fe?tuses had no CHD,including a CHD group of 40 women and a control group of 10 women. The CHD group included 4 subgroups as follows:tetralo?gy of Fallot,ventricular septal defects,persistent truncus arteriosus,and a mixture of relatively rare types of CHD(n=10 each). Samples in the same group were pooled to obtain equal amounts of proteins ,and the iTRAQ proteomic approach was used to identify and quantify the proteins dif?ferentially expressed among these groups. Results We successfully identified 606 proteins,among which 47 showed at least a 1.5?fold difference between the CHD and control groups. Among the 47 proteins,23 and 24 were upregulated and downregulated,respectively. Conclusion Several proteins associated with CHD could be identified by using the iTRAQ proteomic approach ,and various proteins were involved in the pathogenesis of CHD in this study.

Objective To explore the clinical feature,diagnosis,therapy and prognosis of primary cardiac lymphoma.Methods One case of primary diffuse large B-cell lymphoma of the heart was studied.The clinical feature,pathogenesis,diagnosis,therapy and prognosis of primary cardiac lymphoma were further investigated through literatures review.Results Patient,female,64 years old,mainly clinical feature with the activities of chest tightness,gas tight,echocardiogram showed right atrial lesions,ECG of sick sinus syndrome.Right atrial tumor excision by pathological examination confirmed the diagnosis of diffuse large B cell lymphoma(DLBCL),treated with R-CHOP (rituximab,cyclophosphamide,vinorelbine,pirarubicin,prednisone) chemotherapy,cardiac pacemaker implantation,the patient had received 3 cycles of chemotherapy currently,and was at the intermission of chemotherapy.Conclusion Primary cardiac lymphoma is extremely rare.Its pathogenesis remained to be unclear.With non-specific clinical manifestations,the diagnosis is mainly confirmed by histopathological and immunohistochemical staining.It is sensitive to chemotherapy generally,but the special location of the tumor leads to poor prognosis.

OBJECTIVE:To establish a HPLC method for determining the content of tinidazole in Yintal cleaning solu?tion.METHODS:Nova-Pak C 18 column was used.The mobile phase was methanol-water-glacial acetic acid(22∶78∶0.1). Internal standard was metronidazole.The UV detection wavelength was310nm.RESULTS:There was a good linerarity within the concentration range of20～100?g/ml of tinidazole in Yintai solution(r=0.9999).The average recovery was101.2%(n=5,RSD=1.16%).CONCLUSION:This method is simple,fast and accurate.