Objective To evaluate the relationship between the amount of grafted cells and the incidence of acute graft versus host disease (aGVHD) after haploid hematopoietic stem cell transplantation (haplo-HSCT). Methods Data of 68 patients who underwent haplo-HSCT from Jan 2009 to Dec 2013 were analyzed retrospectively. Influences of different factors on the incidence of Ⅲ-Ⅳ degree of aGVHD after HSCT were evaluated. Results 68 patients including 42 males and 26 females were 5/10-9/10 HLA match with 19 father donors, 24 mother donors, 16 sibling donors and 9 children donors. 51 patients not suffered Ⅲ-Ⅳdegree of aGVHD included 32 males and 19 females with the mean age of 20 years old (5-55 years old). 17 patients sufferedⅢ-Ⅳdegree of aGVHD including 10 males and 7 females with the mean age of 23 years old (5-54 years old). There were no significant differences in the amount of the grafted mononuclear cells (MNC) and CD34+cells, and the white blood cell counts (WBC) and platelet count (Plt) recovered time between two groups (P>0.05). However, MNC number was related to CD34+cell number (P<0.05) and WBC recover time (P<0.05), and the CD34+cells number was related to WBC and Plt recover time (P< 0.05). Conclusion The incidence of Ⅲ-Ⅳ degree of aGVHD is unrelated to the amount of grafted MNC, and CD34+cells.

OBJECTIVE: To explore the effection of the pulmonary function of patients of chronic rhinosinusitis (CRS) with asthma which treated with endoscopic sinus surgery (ESS) based comprehensive treatment. METHOD: There were 50 cases of chronic rhinosinusitis with asthma whom met the study criteria. 35 cases enrolled in the tri al group, which treated with endoscopic sinus surgery, and routine perioperative tratment. Another 15 cases as control group which underwent conservative treatment. Both groups underwent the rule treatment of asthma. The main monitoring indexes, which included visual analogue scale (VAS) score, endoscopic Lund-Kennedy score, control of asthma symptoms, the pulmonary function which involved forced expiratory volume in first second (FEV1), forced vital capacity (FVC), the ratio of forced expiratory volume in first second and forced vital capacity (FEV1/FVC) and peak expiratory flow (PEF), were measured in the patients of each groups before surgery, follow-up for 1 year and 3-year. RESULT: Our study found that the VAS score of CRS with asthma was significantly negatively correlated with FEV1 and PEF (P < 0.05), endoscopic Lund-Kennedy score was significantly negatively correlated with PEF (P < 0.05); After the trial group underwent ESS based comprehensive treatment, the improvement of VAS score and endoscopic Lund-Kennedy score of postoperative compared with preoperative and the same period in the control group were significantly (P < 0.05). The difference of the postoperative asthma control rate of trial group after 1 year and after 3 years, respectively, compared with the same period control group were statistically significant (P < 0.05). The preoperative FEV1, FVC, FEV1/FVC and PEF of trial group compared with preoperative were significantly (P < 0.05). Even the difference of them compared with the same period control group were significantly (P < 0.05), except the FVC in the follow-up 3 years (P = 0.088). CONCLUSION The CRS may aggravate asthma symptoms and affect negatively the pulmonary function, and poor asthma control or aggravate may exacerbate the CRS in the course of CRS with asthma patient. With ESS based on combined therapy, it can improve the condition of CRS significantly and improve the control of asthma symptoms and pulmonary function else.
Adolescent ; Adult ; Asthma ; complications ; Chronic Disease ; Endoscopy ; Female ; Humans ; Male ; Middle Aged ; Nose ; surgery ; Pulmonary Ventilation ; physiology ; Rhinitis ; complications ; surgery ; Sinusitis ; complications ; surgery ; Young Adult

Objective To analyze the drug resistance status of mycobacterium tuberculosis in patients with double immunization of human immunodeficiency virus (HIV) and tuberculosis (TB) by phage bioassay (PhaB),and to optimize the control strategy.Methods One hundred and twelve cases of HIV/TB infected patients.in Chongqing Ninth People's Hospital were treated with PhaB method,and the drug susceptibility testing results were compared with 208 cases of simple pulmonary tuberculosis patients.Results The anti-tuberculosis drug resistance rate of HIV/TB patients was lower than that of simple pulmonary tuberculosis patients.The resistance rates of 5 common anti-tuberculosis drugs in HIV/TB patients were 7.14％ of isoniazid (INH),7.14％ of pyrazinamide (PZA),5.36 ％ of rifampicin(RFP) streptomycin(SM),and 4.46 ％ of ethambutol (EMB),compared with simple pulmonary tuberculosis(resistance rates of RFP were 17.31％,IN H 13.46 ％,PZA 11.54 ％,EMB 10.58 ％,SM 9.62 ％),RFP resistance rate of HIV/TB infected patients was lower(P＜0.05).There was no significant difference between two groups in the other four anti-tuberculosis drug(P＞0.05).The coincidence rate with the absolute concentration method were INH 96.4％,RFP 98.2％,PZA 96.4％,EMB 93.8％ and SM 96.4％,respectively.Conclusion The resistance rate of mycobacterium tuberculosis to RFP in patients with HIV/TB infection in this region is lower than that in patients with common pulmonary tuberculosis,which is related to the good medication compliance of these patients.PhaB has the characteristic of fast,simple,without special equipment,it can be used as a rapid screening of mycobacterium tuberculosis drug resistance method.

OBJECTIVETo set up a feasible method for detection of objective genes from paraffin-embedded tissues of oral leukoplakia (OLK).

METHODSTwenty-five pieces of formalin-fixed, paraffin-embedded tissues of OSCC were selected and ATM gene was detected respectively by 3 methods: the microdissection-nested PCR method, proteinase K-PCR method and conventional phenol-chloroform-PCR method. The positivity rates were compared statistically.

RESULTSThe positivity rates of these 3 methods were 84%, 52% and 64% respectively. Significant difference was found in positivity rate between the microdissection-nested PCR method and the proteinase K-PCR method (P = 0.032).

CONCLUSIONSThe microdissection-nested PCR method merits recommendation because it is more efficient, easy to perform and has the advantage of less sample amount.

Ataxia Telangiectasia Mutated Proteins ; Cell Cycle Proteins ; genetics ; DNA-Binding Proteins ; genetics ; Humans ; Leukoplakia, Oral ; genetics ; Paraffin Embedding ; Polymerase Chain Reaction ; methods ; Protein-Serine-Threonine Kinases ; genetics ; Tumor Suppressor Proteins ; genetics

Objective To report the experience of lung transplantation with size reduced graft lung.Methods Four cases receiving lung transplantation with size reduced graft lung were analyzed retrospectively.In case 1,left lung transplantation combined with contra-hteral lung volume mduction.In case 2,right lung transplantation Wag individually performed with partially msecfion of upper lobe of graft lung.In case 3.bilateral sequential lung transplantation wag performed using graft lung with partially resection of bilateral upper lobes.In the remained ease,bilateral sequential lung tansplantation was performed using graft lung with resection of right lower lobe.Results All the size reduced graft lungs had good functions during the peri-operation period.Case 1 and case 2 still survived without obvious complication.Case 3 experienced temporary air leak on the 5th day postoperation and cured by water seal drainage but died of abrupt bronchorrhea due to aspergillus infection on the 32th day postoperation.The last cage experienced smoothly recovery excepted fatal virus pneumonia 2 months postopemtion.Conclusion Size reduced graft lungs can be successfully used for transplantation.

OBJECTIVETo investigate individualized one-staged correction of alveolar cleft and lip and nasal deformities secondary to lip cleft.

METHODSThe alveolar cleft and lip and nasal deformities secondary to lip cleft were corrected in one stage.

RESULTSFrom 2004 to 2007, 37 cases were treated. 33 patients were treated successfully with primary healing in bony recipient area. Cancellous bone exposure happened in 3 cases. The wounds healed after debridement and drainage. The cosmetic results were satisfactory.

CONCLUSIONSOne-staged correction of alveolar cleft and the lip and nasal deformities secondary to lip cleft can achieve good results.

Adolescent ; Alveolar Process ; abnormalities ; Child ; Cleft Lip ; complications ; surgery ; Cleft Palate ; complications ; surgery ; Female ; Humans ; Male ; Nose ; abnormalities ; Nose Deformities, Acquired ; surgery

OBJECTIVETo explore a new method of establishing HepG2 cell model of steatosis and observe the expression and significance of nuclear factor erythroid-2p45-related factor 2(Nrf2)/antioxidative response element (ARE) pathway related factors in HepG2 cells of steatosis.

METHODSHepG2 cells were induced with DMEM containing 25% fetal bovine serum, 0.1% MCT/LCT Fat Emulsion and 0.1 mmol/L free fatty acid (FFA) at different stages and the control group cells were cultured with normal DMEM medium. After the cell models were successfully established, lipid droplets in cytoplasm were observed with Oil Red 0 staining, and the triglyceride (TG) accumulation in HepG2 cells were tested by biochemical assay. Intracellular reactive oxygen species (ROS) concentration were detected by flow cytometry. Nitric oxide (NO), superoxide dismutase(SOD), malonyldialdehyde(MDA) and glutathione peroxidase(GSH-Px) were tested by biological reagent kit, while the protein expression of nuclear factor erythroid-2p45-related factor 2(Nrf2), heme oxygenase-1 (HO-1) and

NAD(P)Hquinone oxidoreductase-1(NQO1) were analyzed by Western blot.

RESULTSCompared with that in the control group, red cytoplasmic lipid droplets were visible in model group; TG,ROS, NO, MDA concentration (P < 0.05, P < 0.01) and the protein expression of Nrf2, HO-1 and NQO1 (P < 0.05, P < 0.01)were significantly higher in model group, while SOD, GSH-Px concentration reduced significantly (P < 0.01).

CONCLUSIONThe in vitro cell model of steatosis and oxidative stress was successfully established. The activation of Nrf2/ARE pathway related factors maybe relevant to the overreaction of oxidative stress in HepG2 cells of steatosis.

Antioxidant Response Elements ; Culture Media ; Fatty Acids, Nonesterified ; Fatty Liver ; metabolism ; GA-Binding Protein Transcription Factor ; Glutathione Peroxidase ; metabolism ; Heme Oxygenase-1 ; metabolism ; Hep G2 Cells ; Humans ; Malondialdehyde ; metabolism ; NAD(P)H Dehydrogenase (Quinone) ; metabolism ; NF-E2-Related Factor 2 ; metabolism ; Nitric Oxide ; metabolism ; Oxidative Stress ; Reactive Oxygen Species ; metabolism ; Superoxide Dismutase ; metabolism ; Triglycerides ; metabolism

OBJECTIVETo explore the correlation between angiographically-defined coronary heart disease (CHD) and chronic periodontitis (CP).

METHODS277 cases with CHD (case group) and another 238 with no agiographic evidence of CHD (control group) were compared on their traditional cardiovascular risk factors, as: oral health status and probing depth, clinical attachment level, gingival recession, as well as number of missing teeth. Other related risk factors of CHD were included in a stepwise logistic regression analysis.

RESULTSData from univariate analysis showed that there was significant difference in CP, plasma triglyceride, high density lipoprotein cholesterol (HDL-C), fast glucose, white blood count, hypertension and smoking between patients with CHD and those with out CHD (P < 0.01 - 0.001). Multiple factorial logistic regression analysis showed that CP, hypertension, diabetes mellitus, HDL-C were strongly correlated with the incidence of CHD. Significant dosage-effective response was also found in the relationship between CP and CHD (P < 0.001). Further studies also suggested that the severity of CP paralleled the severity of CHD.

CONCLUSIONThe results indicated that CP might serve as an independent risk factor of CHD and significantly enhance the risk and severity of CHD. Our study suggested that the elimination of probable risk factors in oral cavity was indispensable during the process of the prevention of CHD in order to prevent acute coronary events.

Angiography ; Biomarkers ; blood ; Case-Control Studies ; Chronic Disease ; Coronary Disease ; epidemiology ; Humans ; Oral Health ; Periodontitis ; epidemiology ; Risk Factors

OBJECTIVETo explore the semen quality of the workers exposed to the xenoestrogen bisphenol A (BPA) was explored.

METHODSA cross-sectional study of 20 BPA exposed and 16 control workers with similar age, physical activities was performed. Tests included quantifying BPA in blood samples and investigating the quantity and quality of semen. Semen parameters were determined with the method recommended by WHO.

RESULTS94.4% exposed workers were found BPA in blood, and the median was 101.94 microg/L. However, only 18.8% control subjects were found BPA in blood, and the median level was 0 microg/L. The sperm density of exposed workers [(68.65 +/- 44.00) x 10(6)/ml] was significantly lower than that of control [(118.56 +/- 98.36) x 10(6)/ml]. Relationship analysis showed the positive relationships (r = 0.44, P < 0.01) between the sperm with quick forward progression and BPA level in blood, negative relationships between the percentage of normal sperm and BPA level in blood (r = -0.62, P = 0.01).

CONCLUSIONBPA could affect the sperm density, and may influence the semen quality. More research should be performed on the effect and the mechanism of BPA on man.

Benzhydryl Compounds ; Chi-Square Distribution ; Cross-Sectional Studies ; Humans ; Male ; Occupational Exposure ; adverse effects ; Phenols ; adverse effects ; Pilot Projects ; Semen ; drug effects ; Semen Analysis

OBJECTIVETo observe the effects of electroacupuncture (EA) on the apoptosis of brain tissue cells and the expression of Caspase-3 in the rats with cerebral-cardiac syndrome (CCS).

METHODSA total of 70 healthy SD rats were selected. Ten was randomly recruited as the sham-operation group, and the rest were used for CCS model preparation. Thirty successfully modeled rats were divided into the model group, the EA group, and the non-EA group, 10 in each group. The model was prepared using injecting collagenase + heparin into the caudate nucleus. Equal volume of 0.9% sodium chloride injection was injected to rats' caudate nucleus in the sham-operation group. EA was started on the 1st day of modeling. Shuigou (GV26), Fengfu (GV16), Neiguan (PC6), and Xinshu (BL15) were needled in the EA group. Four points in the hips were needled in the non-EA group. The EA needling lasted for 20 min each time, once daily, for 3 successive times. No EA was administered to the sham-operation group or the model group. The apoptosis of brain tissue around the hematoma and the expression of Caspase-3 were detected using TUNEL and immunochemical assay.

RESULTSTUNEL cells could be occasionally seen with fewer Caspase-3 expression in the sham-operation group. More TUNEL positive cells appeared in the tissue around the hematoma of the model group with a large amount of Caspase-3 expression. The TUNEL positive cells and Caspase-3 expression were obviously less in the EA group than in the model group and the non-EA group (P < 0.01).

CONCLUSIONSEA could inhibit the apoptosis of brain tissue cells in CCS rats. Its mechanisms might be associated with down-regulating the Caspase-3 expression of the brain tissue around the hematoma.

Animals ; Apoptosis ; Brain ; metabolism ; Caspase 3 ; metabolism ; Cerebral Hemorrhage ; complications ; metabolism ; pathology ; Electroacupuncture ; Heart Diseases ; etiology ; metabolism ; pathology ; Rats ; Rats, Sprague-Dawley