Air Pollutants, Occupational ; Fuzzy Logic ; Occupational Exposure ; prevention & control ; Occupational Health Services ; Paint ; Risk Assessment

Catheter Ablation ; adverse effects ; Heart Septum ; surgery ; Humans

Objective To observe the effects of AMPK activation on cancer cell apoptosis and to investigate the underlying mechanism and its significance to cancer therapy.Methods Human cervical cancer HeLa-S3 cells were divided into six groups:DMSO control,5 μmol/L CD437,CD437+Compound C,CD437+AICAR,Compound C,AICAR.Apoptosis rates in every groups induced by CD437 and other factors were determined by FACS and confirmed with clave Caspase-3 expression by Western blot analysis.Finally,apoptosis rates were detected in HeLa-S3 cells induced by the above factors after wt-LKB1 reintroduction by plasmid transfection.Results FACS results showed that treatment with 5 μmol/L CD437 for 8 hours could induce Hela-S3 cells apoptosis by (87.42±5.95) ％.Co-incubation with 20 μmol/L compound C enhanced apoptotic effect of CD437 by (86.42±5.95) ％,while co-incubation with 2 mmol/L AICAR markedly reduced cell apoptosis induced by CD437 [(51.04±8.26) ％] (P ＜ 0.05).Re-introduction of wt-LKB1 by plasmid transfection attenuated the protective effect of AICAR in CD437 induced apoptosis.Conclusion Endogeneous AMPK activation exerts a protective effect in HeLa-S3 ceils.This effect could be attenuated by re-introduction of wt-LKB1.

Objective To investigate the imaging features of Budd-Chiari syndrome(BCS) on 64 slice spiral computed tomography(64SCT) and the diagnostic value of 64SCT for BCS.Methods Twenty-nine patients diagnosed as BCS by 64SCT were retrospectively included into this study and all the patients were researched by digital substraction angiography(DSA).Two abdominal radiologists analyzed the CT imaging features of BCS,paying attention to the vascular lesion,the morphology abnormality of the liver and the degree of portal hypertension,with review of DSA findings.Results ①The accuracy of 64SCT for BCS was 93.1%(27/29),and there were 2 false positive cases and no false negative case.The accuracy of 64SCT for those patients with thrombosis of inferior vena cava(IVC) and(or) hepatic vein(HV) was high as compared to those with stenosis of IVC and(or) HV.②The morphology abnormality of the liver included hepatomegaly(24 cases),low attenuation(27 cases) and inhomogeneous pattern of parenchymal contrast enhancement(5 patients in arterial phase and 19 patients in portal vein phase).③The images of all the patients showed the features of portal hypertension.Conclusion The accuracy of 64SCT for BCS is satisfactory and the false negative is seldom.The 64SCT could accurately display the morphology abnormality of the liver and the compensatory circulation in BCS patients.For those patients with stenosis of IVC and(or) HV,however,the diagnostic power of 64SCT is limited.

Objective To evaluate the feasibility of automatic tube current modulation(ATCM) in craniocervical artery CT angiography with 256-slice CT,while maintaining the image quality.Methods Sixty patients undergoing craniocervical artery CTA were enrolled into this study,and were divided into conventional tube current group(250 mAs,group A,n=30) and low tube current group(ATCM,group B,n=30).The signal-to-noise ratio(SNR),contrast-to noise ratio(CNR),volume CT dose index(CTDIvol) and effective dose(ED) were evaluated respectively.Results The mean image SNR was(37.47?9.39) and(36.85?7.96) for group A and group B respectively,and was not significantly different(P=0.79).The mean image CNR was(32.13?9.06) and(31.86?7.66) for group A and group B respectively,and was not significantly different(P=0.90).The CT volume dose index(CTDI) was 16.5 mGy and 410.55?0.94mGy for group A and group B respectively,and was significantly different(P

Objective:To study and promote the quality standard for Shuangbai Quyu powder. Methods:A microscopic identifi-cation method was performed to identify Phellodendri amurensis cortex, Rhei radix et rhizoma, Platycladi cacumen and Andrographis herba. TLC was performed to identify Phellodendri amurensis cortex, Andrographis herba and Rhei radix et rhizoma. HPLC was used to determine the content of berberine hydrochloride in Phellodendri amurensis cortex. and the lower limit was determined. Results: The microscopic identification method was simple. The spots in TLC were distinct without any interference from the negative coutrol. Ber-berine hydrochloride had a good linear range of 0. 010-0. 400 μg(r=0. 999 9). The average recovery was 99. 51%, and RSD was 0. 31% (n=6). Conclusion:The method can be used for the quality control of Shuangbai Quyu powder effectively,which is beneficial to the quality standard promotion of hospital preparations.

Objective:To observe the expression of B7-H4 in experimental autoimmune myocarditis (EAM).Methods:BALB/c mice were randomly divided into 2 groups:the control group and the experimental group.The mice of experimental group were injected with myosin to establish EAM models , while the mice of control group were injected with complete Freund 's adjuvant and normal saline.All the mice were killed separately at the 14th,21st,30th and 45th day for lymphocyte proliferation assay ,hematoxylin-eosin staining,immunohistochemical staining and real-time PCR.Results:The inflammation infiltration of heart was most serious at the 14th and 21st day,then it was gradually relieved with time;the results of lymphocyte proliferation assay and real-time PCR were similar to that of the inflammation infiltration of heart ,which were in high level at the 14th and 21st day,and they were both higher than that of the control group ( P<0.05 );B7-H4 protein were only detected in the experimental group ,and it was constantly expressed during the whole experiment on the endothelium of heart with myocarditis.Conclusion:B7-H4 participates in the progress of EAM ,and it may be a new way of studying the mechanism of myocarditis.

Objective To assess the outcome of liver transplantation in patients over sixty years of age,in order to attempt to expand the indications of liver transplantation. Methods From August,2000 to January,2002, the clinical data of 36 patients who underwent liver transplantation in our department were analysed retrospectively. Of the 36 cases, the data (operating time, the length of hospitalization, rejection rate and ICU stay days after operation) of 5 patients whose age were ≥60 years (elderly group) and the data of 31 patients whose age were under 60 years (

Objective To investigate the application value of the Perclose Proglide vascular devices in the thoracic endovascular aortic repair (TEVAR) of aortic dissection.Methods Retrospective analysis of 106 patients who underwent TEVAR for Standford B type aortic dissection were performed.The femoral lumen was measured by CTA be fore,1 month and 1 year after TEVAR.Results A total of 223 Perclose Proglide vascular closure devices were used in the 106 patients,including 97 patients with 2 devices,7 patients with 3 devices,2 patients with 4 devices.The puncture femoral artery diameters had no significant differences between before and 1 month,1 year after TEVAR (all P ＞0.05).Conclusion Per close Proglide vascular closure devices can be effectively and safely used in the TEVAR,which has little influence on the femoral artery diameter,and is worth to be applied in the clinics extensively.

Objective To observe the changes in hypersplenism after orthotopic liver transplantation(OLT) and investigate the effect of OLT on hypersplenism. Methods Based on the clinical data of 14 eligible OLT patients operated on in our hospital during two and a half years, an analysis of the pre operative values of the thickness of the spleen, blood WBC and PLT count was made,and the postoperative pattern of changes of portal flow velocity was observed. Results Blood WBC and PLT count returned to normal 1 month after the operation, and the thickness of spleen reduced about 17.0% in the first month , but had no additional change at 1 year later. Portal flow velocity increased significantly 1 month after operation , then decreased slowly in the first year. Portal flow velocity , blood WBC and PLT count were all significantly related to the thickness of the spleen. Conclusions The high velocity of portal flow after OLT was mostly attributed to increased flow from the splenic vein; the main cause of the decrease in the level of blood WBC and PLT in hypersplenism before operation is augmentation of splenic volume; the recuperation of hypersplenism after OLT relies on the extent of reversion of splenic volume. It is not necessary to perform splenectomy in patients with hypersplenism when they receive OLT.