12E7 Antigen ; Adult ; Antigens, CD ; metabolism ; Cell Adhesion Molecules ; metabolism ; Chondrosarcoma, Mesenchymal ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Hemangiopericytoma ; pathology ; Humans ; Lymphoma ; pathology ; Male ; Nasal Cavity ; Neuroectodermal Tumors, Primitive ; pathology ; Nose Neoplasms ; metabolism ; pathology ; surgery ; Vimentin ; metabolism ; Young Adult

Objective To investigate clinicopathological features of endometriosis-associated epithelial ovarian carcinoma.Methods Retrospective follow-up study,clinicopathological data from patients with ovarian epithelial carcinoma were retrieved,analyzed and compared.Among the 727 cases,34 were found to originate from endometriosis (group A),33 were found to have co-existing ovarian endometriosis (group B),and the remaining 660 had no ovarian endometriosis at all (group C).Result Seven hundred and twenty-seven epithelial ovarian carcinoma patients were identified and their ehnicopathological data retrieved.Sixty-seven (9.2%) of these cases were found to have coexisting endometriosis.The frequency of malignant tumors arising from ovarian endometriosis in this case series was estimated to be 0.87% (34/3890).The mean (standard deviation) age in groups A,B,and C were(47.2±1.3),(47.8±1.2),(51.2±0.4) years,respectively,with patients in group C being significantly older (P = 0.013).Patients with coexisting ovarian endometriosis were mostly diagnosed at stage Ⅰ (P = 0.000)and having subtype of clear-cell (P =0.000),while other patients were mostly diagnosed at stage Ⅲ (P =0.001),and having subtype of serous carcinoma (P =0.000).The estrogen receptor (ER) positivity was significantly lower in groups A and B than that in group C (22.2%,31.6% vs 43.9%;P =0.018),but the difference in positivity of progestogen receptor among the three groups did not reach statistical significance (22.2%,15.8% vs 35.5%;P =0.082).While the five-year overall survival rate for all patients was 55.6%,significant difference in overall survival among the three groups was found 78.9%,92.8%,51.9%,respectively,for groups A,B and C (P =0.000).Conclusion Patients of endometriosis-associated epithelial ovarian carcinoma,especially patients with tumors arising from endometriosis,were found to be younger,having a significant lower stage and a better survival,and were mostly diagnosed with the subtype of clear-cell.

Objective To explore the effects of combination of capecitabine and docetaxel for the treatment on advanced breast cancer and their influence on tumor biomarkers.Methods The 75 cases with advanced breast cancer were randomly recruited from December 2010 to December 2015 in our hospital,and they were divided into the observation group (38 cases) and control group (37 cases) according to the admission time,the patients in control group were treated with docetaxel,while patients in the observation group were treated with combination of capecitabine and docetaxel,the clinical efficacy before and after treatment was observed,and the changes of serum CEA,glucose CA125,CA15-3 and adverse reaction were compared between two groups.Results The effective rate (RR) of the control group was 40.54% (15/37),and the disease control rate (DCR) was 67.57% (25/37).The RR was 63.16% (24/38) and DCR was 86.84% (33/38)in the observation group.There was significant difference between the two groups (P<0.05).No statistically significant difference was observed between the two groups on the the levels of serum tumor biomarker,CEA,CA125 and CA15-3 before treatment (P>0.05);After treatment,the levels of CEA,CA125 and CA15-3 in both two groups were significantly lower than those before treatment (P<0.05);Moreover,after treatment,the observation group of three tumor biomarkers were significantly lower than those of the control group (P<0.05).Conclusion Combination of capecitabine and docetaxel in the treatment of advanced breast cancer can reduce the serum tumor biomarkers significantly,attenuate the side effects,and the patients are in the good tolerance,it can be widely recommended in clinical use.

OBJECTIVE:To discuss the contents and importance of clinical pharmaceutical care.METHODS:Our experiences in carrying out clinical pharmaceutical care in our hospital were analyzed through exemplification.RESULTS & CONCLUSION:Clinical pharmaceutical care can help improve the medical quality and reduce medical risks.Pharmacist system should be established in hospital to support the work of clinical pharmacists.

There are a lot of medical colleges which carry out foreign student medical education in our country. On the basis of clinical theory situation and problems of foreign students medical education,Xuzhou Medical College implemented a number of reform measures and achieved good teaching effect.These measures included writing new syllabus of oversea students and reforming multiform teaching methods and the pattern of examination and evaluation for clinical theory.

As the manual muscle testing is not sensitive enough to reflect the change of muscle strength, more and more people begin to use isokinetic dynamometry and handheld dynamometer for quantitative tests of muscle strength measurement. This paper briefly introduced the history of clinical approaches of muscle strength measurement, compared the advantages and disadvantages of various quantitative mus-cle strength testing, on which the specific techniques of clinical muscle strength measurement were discussed.

The study is aimed to explore a rapid method to extract DNA from fried Chinese medicinal products. The alkaline lysis buffer was made of sodium hydroxide, 1% PVP and 1% TritonX-100 and Tris-HCl solution was neutralized, through heat cracking and neutralization two step to extract DNA from processed and prepared products of traditional Chinese medicine. Then universal primes were used to amplify PCR products for fired Chinese medicinal materials. The results indicated the optimized alkaline lysis method for extracting DNA is quick and easy. Extracting of the different processed Sophora japonica of DNA concentration was (420.61 ± 123.91) g x L(-1). Using 5% Chelex-100 resin purification can improve the DNA concentration. Our results showed that the optimized alkaline lysis method is suitable for Chinese medicinal materials for quickly DNA extraction.
Alkalies ; chemistry ; Chemical Fractionation ; methods ; DNA, Plant ; genetics ; isolation & purification ; Hydrolysis ; Plants, Medicinal ; chemistry ; classification ; genetics ; Polymerase Chain Reaction ; Sophora ; chemistry ; classification ; genetics

Objective To retrospectively analyze the lower extremity deep vein thrombosis prevention in patients with spinal cord injury (SCI). Methods A total of 115 SCI patients in our department from April to May, 2015 were included. The clinical symptoms, lower limb deep vein ultrasonic testing, laboratory examination were collected to analyze the occurrence, prevention measures, the thrombus location and management of deep venous thrombosis (DVT) in lower limbs. Results Forty-three patients had thromboprophylaxis in other hospitals before admission, and 105 patients in our department after admission, in which, nine cases were with clinical symptoms in other hospitals and three cases in our department. No pulmonary embolism occurred in them. There was no significant difference in most laboratory index-es between patients with DVT and without DVT in lower limbs (P>0.05). Five patients were with DVT in lower limbs in 43 patients who had thromboprophylaxis, and four cases in 72 patients who did not have thromboprophylaxis. No relationship was found between thrombo-prophylaxis and DVT in lower limbs (χ2=0.663, P=0.415). Five patients were with DVT in lower limbs in 53 patients with complete SCI, and four cases in 59 patients with incomplete SCI. No relationship was found between the severity of SCI and DVT in lower limbs in other hospitals (χ2=0.028, P=0.867). Conclusion DVT in lower limbs could be also occurred in patients who accepted thromboprophylaxis. Labo-ratory indexes are inadequate for the prediction and diagnosis specificity of DVT in lower limbs.

Study design Survey of expert opinion, feedback and final consensus. Objective To describe the development and the variables included in the International Spinal Cord Injury (SCI) Spinal Interventions and Surgical Procedures Basic Data set. Setting International working group. Methods A committee of experts was established to select and define data elements. The data set was then disseminated to the appropriate committees and organizations for comments. All suggested revisions were considered and both the International Spinal Cord Society and the American Spinal Injury Association endorsed the final version. Results The data set consists of nine variables: (1) Intervention/ Procedure Date and start time (2) Non-surgical bed rest and external immobilization, (3) Spinal intervention-closed manipulation and/or reduction of spinal elements, (4) Surgical procedure-approach, (5) Date and time of the completion of the intervention or surgical closure; (6) Surgical procedure-open reduction, (7) Surgical procedure-direct decompression of neural elements, and (8 and 9) Surgical procedure- stabilization and fusion (spinal segment number and level). All variables are coded using numbers or characters. Each spinal intervention and procedure is coded (variables 1 through 7) and the spinal segment level is described (variables 8 and 9). Sample clinical cases were developed to illustrate how to complete it. Conclusion The International SCI Spinal Interventions and Surgical Procedures Basic Data Set was developed to facilitate comparisons of spinal interventions and surgical procedures among studies, centers and countries.

There is autonomic nerve dysfunction after cervical spinal cord injury. Fluctuation of blood pressure and heart rate was found as orthostatic hypotension and bradycardia.